BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.929459.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/929459 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended\nfor legal use, since it may contain inaccuracies. Neither the data\nContributor, ERD, NOAA, nor the United States Government, nor any\nof their employees or contractors, makes any warranty, express or\nimplied, including warranties of merchantability and fitness for a\nparticular purpose, or assumes any legal liability for the accuracy,\ncompleteness, or usefulness, of this information.
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL summary String The affinity of Synechococcus (WH8102 and WH5701) alkaline phosphatases for different DOP model substrates was examined in laboratory experiments through its ability to inhibit the hydrolysis of the fluorogenic substrate 4-methylumbelliferyl phosphate (MUF-P). These data were collected as part of a study of \"Dissolved organic phosphorus bond-class utilization by Synechococcus\" (Waggoner et al., 2024).\n \nStudy Abstract:\nDissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP's importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5'-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5'-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5'-triphosphate, and then adenosine 5'-monophosphate. The strains' exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus' exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.
attribute NC_GLOBAL title String [Synechococcus DOP Displacement Experiment] - Laboratory-cultured Synechococcus (WH8102 and WH5701) MUF-P hydrolysis inhibition by dissolved organic phosphorus (DOP) from experiments between 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle)
variable synechococcus_strain String
attribute synechococcus_strain long_name String Synechococcus_strain
attribute synechococcus_strain units String unitless
variable MUF_P_conc float
attribute MUF_P_conc actual_range float 0.5, 0.5
attribute MUF_P_conc long_name String Muf_p_conc
attribute MUF_P_conc units String micromoles per liter (umol L-1)
variable DOP_conc int
attribute DOP_conc actual_range int 0, 100
attribute DOP_conc long_name String Dop_conc
attribute DOP_conc units String micromoles per liter (umol L-1)
variable DOP_substrate String
attribute DOP_substrate long_name String Dop_substrate
attribute DOP_substrate units String unitless
variable MUFP_hydrolysis_trip1 float
attribute MUFP_hydrolysis_trip1 actual_range float 0.0, 193.0
attribute MUFP_hydrolysis_trip1 long_name String Mufp_hydrolysis_trip1
attribute MUFP_hydrolysis_trip1 units String percentage (%)
variable MUFP_hydrolysis_trip2 float
attribute MUFP_hydrolysis_trip2 actual_range float 28.0, 182.0
attribute MUFP_hydrolysis_trip2 long_name String Mufp_hydrolysis_trip2
attribute MUFP_hydrolysis_trip2 units String percentage (%)
variable MUFP_hydrolysis_trip3 float
attribute MUFP_hydrolysis_trip3 actual_range float 20.0, 184.0
attribute MUFP_hydrolysis_trip3 long_name String Mufp_hydrolysis_trip3
attribute MUFP_hydrolysis_trip3 units String percentage (%)

 
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