BCO-DMO ERDDAP
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Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL doi String 10.26008/1912/bco-dmo.929471.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/929471 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL license String The data may be used and redistributed for free but is not intended\nfor legal use, since it may contain inaccuracies. Neither the data\nContributor, ERD, NOAA, nor the United States Government, nor any\nof their employees or contractors, makes any warranty, express or\nimplied, including warranties of merchantability and fitness for a\nparticular purpose, or assumes any legal liability for the accuracy,\ncompleteness, or usefulness, of this information.
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL summary String Marine cyanobacterium Synechococcus (WH8102 and WH5701) laboratory culture in vivo fluorescence and flow cytometry cell counts following growth in high and low phosphate media, as part of the DOP hydrolysis experiments in a study of \"Dissolved organic phosphorus bond-class utilization by Synechococcus\" (Waggoner et al. 2024). \n\nStudy Abstract:\nDissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP's importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5'-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5'-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5'-triphosphate, and then adenosine 5'-monophosphate. The strains' exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus' exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems.
attribute NC_GLOBAL title String [Synechococcus DOP Hydrolysis Experiment - cell counts and IVF] - In vivo fluorescence and flow cytometry cell counts from dissolved organic phosphorus (DOP) hydrolysis experiments with marine cyanobacterium Synechococcus laboratory cultures (WH8102 and WH5701) from 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle)
variable synechococcus_strain String
attribute synechococcus_strain long_name String Synechococcus_strain
attribute synechococcus_strain units String unitless
variable time_day int
attribute time_day actual_range int 0, 24
attribute time_day long_name String Time_day
attribute time_day units String days
variable media_and_phosphate_level String
attribute media_and_phosphate_level long_name String Media_and_phosphate_level
attribute media_and_phosphate_level units String unitless
variable in_vivo_fluor_trip1 float
attribute in_vivo_fluor_trip1 actual_range float 16.5, 589.5
attribute in_vivo_fluor_trip1 long_name String In_vivo_fluor_trip1
attribute in_vivo_fluor_trip1 units String relative fluorescence units (RFU)
variable in_vivo_fluor_trip2 float
attribute in_vivo_fluor_trip2 actual_range float 16.5, 565.5
attribute in_vivo_fluor_trip2 long_name String In_vivo_fluor_trip2
attribute in_vivo_fluor_trip2 units String relative fluorescence units (RFU)
variable in_vivo_fluor_trip3 float
attribute in_vivo_fluor_trip3 actual_range float 16.5, 617.5
attribute in_vivo_fluor_trip3 long_name String In_vivo_fluor_trip3
attribute in_vivo_fluor_trip3 units String relative fluorescence units (RFU)
variable cell_counts_trip1 int
attribute cell_counts_trip1 actual_range int 22435, 309927090
attribute cell_counts_trip1 long_name String Cell_counts_trip1
attribute cell_counts_trip1 units String cells per milliliter (cells mL-1)
variable cell_counts_trip2 int
attribute cell_counts_trip2 actual_range int 44869, 409953052
attribute cell_counts_trip2 long_name String Cell_counts_trip2
attribute cell_counts_trip2 units String cells per milliliter (cells mL-1)
variable cell_counts_trip3 int
attribute cell_counts_trip3 actual_range int 33652, 340458870
attribute cell_counts_trip3 long_name String Cell_counts_trip3
attribute cell_counts_trip3 units String cells per milliliter (cells mL-1)

 
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