Project: Regenerated Nitrogen Name: BCO-DMO Email: info@bco-dmo.org Institution: BCO-DMO InfoURL: https://www.bco-dmo.org/dataset/489420 Source URL: (local files) Unknown tabular digital data Woods Hole, MA, USA ERDDAP, version 2.22, at BCO-DMO https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.graph https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.subset Regenerated Nitrogen We evaluated the role of mussels by adding 15N-labeled NH4+ to an assemblage of tidepools where they were either present at natural abundance levels, or absent through manual removal. \u00a0The role of phototrophs was separately examined by conducting these experiments both during the day and at night. \u00a0The tidal height of pools varied between 1.2 to 1.5 m above Mean Lower Low Water (MLLW). Tidepools were thus isolated from each other as well as the nearshore environment during the low tide period when experiments were conducted. Each experiment included 4 to 5 mussel removal (MR) tidepools (since 2002) and 4 to 5 mussel control (MC) tidepools with natural mussel densities. \u00a0In June 2010, we performed daytime NH4+ tracer experiments and in August 2010 nighttime experiments using the same tidepools. The following year (July 2011) these experiments were repeated with the addition of bottle incubations (see below) to evaluate the effects of suspended tidepool components and extended sampling for 6 days after the initial 15N addition to test for long-term retention of NH4+. \u00a0During the 2011 experiments, unforeseen rain reduced the salinity in some pools by up to 51%, and we have attempted to correct for the expected dilution of NH4+ in our tidepool rate calculations. Because isotope enrichment levels were relatively low, we used the conventional delta notation instead of atom% to describe variations in 15N enrichment (where delta-15NH4+ = {(15N:14N sample \u00f7 15N:14N standard) -1}\u00d71000\u2030, where the standard is atmospheric N2. \u00a0Tracer labeled ammonium chloride (15NH4Cl) was added to the pools to approximate a 1000\u2030 enrichment in 2010 (doubling the 15N-NH4+ concentration) and a 2000\u2030 enrichment in 2011(tripling the 15N-NH4+ concentration). 15N natural abundance is only 0.365% and these tracer additions thus had a negligible effect on the overall NH4+ concentrations increasing them by only ~0.4% and ~0.8%, respectively. Tidepool volumes were estimated spectrophotometrically using varying concentrations of food dye (Pfister 1995). Together with estimates of NH4+ concentration (from 2009 data), we estimated the tracer addition required to achieve the targeted 15N enrichments. However, the actual initial enrichments varied substantially, 684.4 - 2406.4\u2030 in 2010 and 781.4 - 3880.2\u2030 in 2011, likely due to error in tidepool volume estimation and natural variations in initial NH4+ concentrations. Fortunately, we sampled immediately following each tracer addition allowing for the determination of the true initial 15N enrichment. \u00a0 Prior to tracer addition at ebb tide, 100 mL of tidepool water was syringe- filtered (Whatman GF/F) into separate HDPE bottles for natural abundance 15NH4+ and concentration determination. \u00a0To each pool, tracer 15NH4+ was then added and distributed by stirring with a stick. Water samples were immediately collected for measuring initial 15N enrichment and subsequently at 2, 4, and 6 hour intervals to determine isotope and concentration time courses. All water samples were frozen until analysis. Tidepool oxygen, pH, and temperature (Hach HQ4D) were also collected at ~ 2 h intervals throughout the experiment. In 2011 we also assessed the contribution of the suspended microbial community to NH4+ cycling by enclosing tidepool water in a 250 mL transparent polycarbonate incubation bottle. Following tracer addition, the bottle was filled, then left to float in the tidepool for the duration of the experiment. Samples from bottles were filtered as described both immediately after containment and at the end of the experiment (~6 h later). We assessed macroalgal contribution to NH4+ removal by transplanting two tidepool-dwelling algae species. \u00a0Prionitis sternbergii were sampled 2 weeks prior to the experiment for baseline natural abundance 15N values and transplanted into the pools with Z-Spar Epoxy (Pfister 2007). On the day of the experiment, the red-alga, Corallina vancouveriensis from a single source patch, was also sampled for 15N natural abundance, inserted into pieces of Styrofoam, and floated in each pool. At the end of each experiment (~6 h sampling point), we sampled tidepool particulate organic material (POM) by filtering through combusted GF/F filters until they clogged (~ 600 mL), comparing these samples with POM similarly sampled from the immediate nearshore. \u00a0Floating Corallina spp. samples were collected into clean Eppendorf tubes, and similar sized pieces of Prionitis spp. were collected from each pool into clean foil packets.\u00a0 We evaluated the extent of longer-term 15N tracer retention in 2011 by sampling tidepool water, POM and transplanted Prionitis 1, 3, and 6 days following tracer addition. We sampled at ebb tide and at again at slack water just prior to high tide on the first day after tracer addition (that is, 24 h later) and at slack water prior to high tide on Day 3 and 6.\u00a0 Relevant References: 2014\. Pather, S., C. A. Pfister, M. Altabet, D. M. Post. Ammonium cycling in the rocky intertidal: remineralization, removal and retention. Limnology and Oceanography 59:361-372.\u00a0[http://aslo.org/lo/toc/vol_59/issue_2/0361.htm](\\http://aslo.org/lo/toc/vol_59/issue_2/0361.html\\) DOI for this dataset:\u00a0The role of regenerated nitrogen for rocky shore productivity, Cape Flattery, Washington, 2010 & 2011.\u00a0Handle:\u00a0[http://hdl.handle.net/1912/6420](\\http://hdl.handle.net/1912/6420\\).\u00a0DOI:10.1575/1912/6420 Related Datasets: [filter tracer content](\\https://www.bco-dmo.org/dataset/489283\\) [natural abundance N and C filter content](\\http://www.bco- dmo.org/dataset/489310\\) [tidepool ammonium and mussels](\\https://www.bco-dmo.org/dataset/488860\\) [tidepool incubation ammonium](\\https://www.bco-dmo.org/dataset/489085\\) Unknown https://www.bco-dmo.org/dataset/489420 Unknown Unknown ground condition Complete As needed -124.57 -124.57 48.37 48.37 Uncontrolled bco bco-dmo biological C_pcent chemical d15 d15N data dataset date dmo erddap latitude longitude management N_pcent oceanography office pcent preliminary sample site species station treatment CF Standard Name Table v55 latitude longitude None. https://www.bco-dmo.org/dataset/489420/license BCO-DMO BCO-DMO Unknown Mailing and Physical Address

Unknown

Unknown Unknown Unknown Unknown Unknown info@bco-dmo.org https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.graph Web page to make a customized map or graph of the data HTML Unknown Unknown Unknown Unknown Unknown Unknown Point Point BCO-DMO Adam Shepherd Technical Director Mailing and Physical Address

266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org ERDDAP, version 2.22: get metadata; download data; make graphs and maps. https://www.bco-dmo.org/dataset/489420/license .asc 1 View OPeNDAP-style ISO-8859-1 comma-separated text. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#ASCII_Service https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .csv 1 Download a ISO-8859-1 comma-separated text table (line 1: names; line 2: units; ISO 8601 times). https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .csvp 1 Download a ISO-8859-1 .csv file with line 1: name (units). Times are ISO 8601 strings. https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .csv0 1 Download a ISO-8859-1 .csv file without column names or units. Times are ISO 8601 strings. https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .dataTable 1 A JSON file formatted for use with the Google Visualization client library (Google Charts). https://developers.google.com/chart/interactive/docs/reference#dataparam https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .das 1 View the dataset's metadata via an ISO-8859-1 OPeNDAP Dataset Attribute Structure (DAS). https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Dataset_Attribute_Structure https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .dds 1 View the dataset's structure via an ISO-8859-1 OPeNDAP Dataset Descriptor Structure (DDS). https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Dataset_Descriptor_Structure https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .dods 1 OPeNDAP clients use this to download the data in the DODS binary format. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Data_Transmission https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .esriCsv 1 Download a ISO_8859_1 .csv file for ESRI's ArcGIS 9.x and below (separate date and time columns). https://support.esri.com/technical-article/000012745 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .fgdc 1 View the dataset's UTF-8 FGDC .xml metadata. https://www.fgdc.gov/standards/projects/FGDC-standards-projects/metadata/base-metadata/index_html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .geoJson 1 Download longitude,latitude,otherColumns data as a UTF-8 GeoJSON .json file. http://wiki.geojson.org/Main_Page https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .graph 1 View a Make A Graph web page. https://coastwatch.pfeg.noaa.gov/erddap/tabledap/documentation.html#GraphicsCommands https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .help 1 View a web page with a description of tabledap. https://www.opendap.org/pdf/ESE-RFC-004v1.2.pdf https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .html 1 View an OPeNDAP-style HTML Data Access Form. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#WWW_Interface_Service https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .htmlTable 1 View a UTF-8 .html web page with the data in a table. Times are ISO 8601 strings. https://www.w3schools.com/html/html_tables.asp https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .iso19115 1 View the dataset's ISO 19115-2/19139 UTF-8 .xml metadata. https://en.wikipedia.org/wiki/Geospatial_metadata https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .itx 1 Download an ISO-8859-1 Igor Text File. Each response column becomes a wave. https://www.wavemetrics.net/doc/igorman/II-09%20Data%20Import%20Export.pdf https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .json 1 View a table-like UTF-8 JSON file (missing value = 'null'; times are ISO 8601 strings). https://www.json.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .jsonlCSV1 1 View a UTF-8 JSON Lines CSV file with column names on line 1 (mv = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .jsonlCSV 1 View a UTF-8 JSON Lines CSV file without column names (mv = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .jsonlKVP 1 View a UTF-8 JSON Lines file with Key:Value pairs (missing value = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .mat 1 Download a MATLAB binary file. https://www.mathworks.com/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .nc 1 Download a flat, table-like, NetCDF-3 binary file with COARDS/CF/ACDD metadata. https://www.unidata.ucar.edu/software/netcdf/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .ncHeader 1 View the UTF-8 header (the metadata) for the NetCDF-3 .nc file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .ncCF 1 Download a NetCDF-3 CF Discrete Sampling Geometries file (Contiguous Ragged Array). https://cfconventions.org/Data/cf-conventions/cf-conventions-1.8/cf-conventions.html#discrete-sampling-geometries https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .ncCFHeader 1 View the UTF-8 header (the metadata) for the .ncCF file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .ncCFMA 1 Download a NetCDF-3 CF Discrete Sampling Geometries file (Multidimensional Array). https://www.ncei.noaa.gov/netcdf-templates https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .ncCFMAHeader 1 View the UTF-8 header (the metadata) for the .ncCFMA file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .nccsv 1 Download a NetCDF-3-like 7-bit ASCII NCCSV .csv file with COARDS/CF/ACDD metadata. https://coastwatch.pfeg.noaa.gov/erddap/download/NCCSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .nccsvMetadata 1 View the dataset's metadata as the top half of a 7-bit ASCII NCCSV .csv file. https://coastwatch.pfeg.noaa.gov/erddap/download/NCCSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .ncoJson 1 Download a UTF-8 NCO lvl=2 JSON file with COARDS/CF/ACDD metadata. https://nco.sourceforge.net/nco.html#json https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .odvTxt 1 Download longitude,latitude,time,otherColumns as an ISO-8859-1 ODV Generic Spreadsheet File (.txt). https://odv.awi.de/en/documentation/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .subset 1 View an HTML form which uses faceted search to simplify picking subsets of the data. https://en.wikipedia.org/wiki/Faceted_search https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .tsv 1 Download a ISO-8859-1 tab-separated text table (line 1: names; line 2: units; ISO 8601 times). https://jkorpela.fi/TSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .tsvp 1 Download a ISO-8859-1 .tsv file with line 1: name (units). Times are ISO 8601 strings. https://jkorpela.fi/TSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .tsv0 1 Download a ISO-8859-1 .tsv file without column names or units. Times are ISO 8601 strings. https://jkorpela.fi/TSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .wav 1 Download a .wav audio file. All columns must be numeric and of the same type. https://en.wikipedia.org/wiki/WAV https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .xhtml 1 View a UTF-8 XHTML (XML) file with the data in a table. Times are ISO 8601 strings. https://www.w3schools.com/html/html_tables.asp https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.html .kml 1 View a .kml file, suitable for Google Earth. https://developers.google.com/kml/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.graph .smallPdf 1 View a small .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.graph .pdf 1 View a standard, medium-sized .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.graph .largePdf 1 View a large .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.graph .smallPng 1 View a small .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.graph .png 1 View a standard, medium-sized .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.graph .largePng 1 View a large .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.graph .transparentPng 1 View a .png image file (just the data, without axes, landmask, or legend). http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_489420.graph None 20260611 BCO-DMO Adam Shepherd Technical Director Mailing and Physical Address

266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org Content Standard for Digital Geospatial Metadata FGDC-STD-012-2002 universal time None. https://www.bco-dmo.org/dataset/489420/license