[Carb microarray analysis Atlantic 2019] - Carbohydrate microarray (epitope) analyses of POM-derived carbohydrates in the Western North Atlantic Ocean in May 2019 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation)

Project: BCO-DMO Name: BCO-DMO Email: info@bco-dmo.org Institution: BCO-DMO InfoURL: https://osprey.bco-dmo.org/dataset/985786 Source URL: (local files) [Carb microarray analysis Atlantic 2019] - Carbohydrate microarray (epitope) analyses of POM-derived carbohydrates in the Western North Atlantic Ocean in May 2019 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation) Unknown tabular digital data Woods Hole, MA, USA ERDDAP, version 2.22, at BCO-DMO https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_985786_v1.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_985786_v1.graph BCO-DMO Marine dissolved organic carbon (DOC) is one of the largest actively-cycling carbon reservoirs on earth—comparable in magnitude to atmospheric CO2 (Hansell 2013)—and thus is an essential component of the global carbon cycle. DOC has a multitude of sources, including phytoplankton productivity, grazing, excretion, solubilization from particulate organic matter (POM), viral lysis, and riverine input; the major DOC sink in the ocean is consumption by heterotrophic microbial communities (Carlson & Hansell 2015). Measurement of bulk DOC characteristics such as 14C age and molecular size have demonstrated that the high molecular weight (HMW) DOC fraction is generally younger and more biologically reactive than the low molecular weight (LMW) fraction (e.g. Guo et al. 1996; Walker et al. 2016). We know that a substantial fraction of HMW DOC consists of carbohydrates, including neutral sugars, and that its concentration is lower in the deep ocean than in the upper mesopelagic/surface ocean (Benner & Amon 2015). Characterization of the HMW DOC fraction has primarily used chemical measurements that provide information about monomeric constituents (Benner et al. 1992; Kaiser & Benner 2009), but yield no information on the order in which such constituents are linked together, or about the 3D structure of the intact HMW DOC. Beyond these observations and measurements, however, the specific factors controlling the rate, location, and extent to which DOC is transformed and remineralized by heterotrophic microbial communities in the ocean are still not well understood. A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). To better contextualize this hydrolysis, bulk water characterization of the deep chlorophyll maximum (DCM), oxygen minimum zone (OMZ), and bottom waters used in our mesocosm incubation experiments was performed. This dataset includes the measurement of carbohydrate microarray (epitope) analyses of POM-derived carbohydrates from bulk waters collected in the western North Atlantic Ocean aboard R/V Endeavor (EN638) during May 2019. Note that the carbohydrate microarray data are only semiquantitative; while comparisons can be made for the abundance of a given epitope between stations and depths, the signal intensity cannot be used to compare signals of different epitopes, since the binding affinity for individual epitopes differs between antibodies. Note also that analyses were performed for all sampled depths at all stations. There was, however, no positive signal at depths that are shown as "0" in this dataset. Unknown https://osprey.bco-dmo.org/dataset/985786 20190515 20190525 ground condition Complete As needed -75.67819 -53.3949 42.83954 34.50102 Uncontrolled earth science atmosphere ocean biosphere biology environment Uncontrolled latitude longitude time depth None. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO BCO-DMO Unknown Mailing and Physical Address

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Unknown Unknown Unknown Unknown Unknown info@bco-dmo.org https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_985786_v1.graph Web page to make a customized map or graph of the data HTML Unknown Unknown Unknown Unknown Unknown Unknown Point Point BCO-DMO Adam Shepherd Technical Director Mailing and Physical Address

266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org ERDDAP, version 2.22: get metadata; download data; make graphs and maps. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. 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266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org Content Standard for Digital Geospatial Metadata FGDC-STD-012-2002 universal time None. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information.