http://lod.bco-dmo.org/id/dataset/680935
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-02-02
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Quantitative PCR data from samples collected on the R/V Sarmiento de Gamboa cruise in the subtropical North Atlantic Ocean between January and March 2011 (Microbial associations in zooplankton project)
2017-02-02
publication
2017-02-02
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-04-04
publication
https://doi.org/10.1575/1912/bco-dmo.680935.1
Pia Moisander
University of Massachusetts Dartmouth
principalInvestigator
Mar Benavides
Université de Toulon
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Moisander, P., Benavides, M. (2017) Quantitative PCR data from samples collected on the R/V Sarmiento de Gamboa cruise in the subtropical North Atlantic Ocean between January and March 2011 (Microbial associations in zooplankton project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-02-02 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.680935.1 [access date]
Quantitative PCR targeting Trichodesmium, UCYN-A, UCYN-B, gamma-24774A11, and Het1, based on the nifH gene. Dataset Description: <p>Quantitative PCR targeting Trichodesmium, UCYN-A, UCYN-B, gamma-24774A11, and Het1, based on the nifH gene.</p> Methods and Sampling: <p>Samples were collected from Niskin bottles, filtered, and processed for DNA analyses. For detailed methods see Benavides et al. 2016.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1130495 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1130495
completed
Pia Moisander
University of Massachusetts Dartmouth
508-999-8222
Department of Biology 285 Old Westport Road
North Dartmouth
MA
02747
USA
pmoisander@umassd.edu
pointOfContact
Mar Benavides
Université de Toulon
Institute of Oceanography UM 110
Noumea
98848
New Caledonia
mar_lliure@hotmail.com
pointOfContact
asNeeded
Dataset Version: 1
Unknown
number
project
date
time
station
lat
lon
size_fraction
UCYN_A
Gamma_24774A11
group_B
Tricho
Het1
ISO_DateTime_UTC
PCR
theme
None, User defined
sample identification
project
date
time of day
station
latitude
longitude
filter_size
genetic material concentration
ISO_DateTime_UTC
featureType
BCO-DMO Standard Parameters
Thermal Cycler
instrument
BCO-DMO Standard Instruments
Malaspina_2011
service
Deployment Activity
Subtropical North Atlantic Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Microbial associations in zooplankton: significance for the marine nitrogen cycle
https://www.bco-dmo.org/project/564455
Microbial associations in zooplankton: significance for the marine nitrogen cycle
<p><em>Description from NSF award abstract:</em><br />
Nitrogen (N2) fixation is a key biogeochemical process providing new N for the marine ecosystem. Current estimates of the global rates suggest that the inputs and outputs of N are not in balance, leaving an apparent deficiency in N2 fixation in the oceanic N budget. Based on recovery of N2 fixation gene fragments, however, numerous potential N2-fixing microbes are present in the euphotic layer and below, yet evidence for their contribution to N2-fixation is lacking. The goal of this study is to identify and determine the importance of potentially diazotrophic, heterotrophic prokaryotes occupying a previously unstudied niche in the upper ocean.</p>
<p>This project PIs hypothesize that N2 fixation in zooplankton-associated microbial communities is an important source of new N in the open ocean contributing to the oceanic N budget. In oligotrophic, N-limited open ocean waters, zooplankton are faced with the same low N availability in their diets that limits growth of primary producers and heterotrophic microbes in the open ocean. Zooplankton guts would be a particularly suitable environment for heterotrophic N2-fixers as the nitrogenase enzyme is inhibited by oxygen. In many terrestrial insects, N2 fixation by gut microbes serves as a source of N. The aim of this study is to investigate whether similar associations occur in marine zooplankton that live on N-limited phytoplankton. The PIs will investigate specific associations between copepods from N-limited and non-N limited oceanic waters with microorganisms that are potentially or actively fixing N. They will also investigate the fate of this N to study whether the new N is incorporated into the zooplankton host. The main research objectives are to determine whether:</p>
<p>1. diazotrophic microflora exist in copepods and what is their seasonal and spatial variability,</p>
<p>2. microbial communities associated with copepods are actively fixing N2,</p>
<p>3. diazotroph communities contribute to the N nutrition of their zooplankton hosts.</p>
<p>Monthly samples will be collected in the oligotrophic Sargasso Sea, in association with BATS sampling cruises. In addition, experiments and field studies will be conducted in Bermuda during the summer. For comparison to a site that is not severely N limited, and for methods development, additional studies will be carried out in coastal Gulf of Maine waters. Selected copepod species and their fecal pellets will be analyzed for the presence of nifH genes and their expression using cloning and sequencing and Reverse Transcriptase-PCR. Quantitative PCR will be employed to investigate the seasonal distributions of dominant phylotypes. Anaerobic cultivation will be used for the enrichment of the gut microflora. Zooplankton will be incubated with 15N2 to quantify microbial N2 fixation activity and its fate of N through zooplankton overall 15N content and nanoSIMS determination of 15N localization. Products of this study will include novel microbial isolates or consortia, evidence regarding taxa-specific expression of N2 fixation in these microhabitats, and a first estimate for the importance of this process for the oceanic N budget.</p>
<p>This project addresses fundamental issues regarding the sources of oceanic nitrogen and will test hypotheses regarding previously overlooked diazotrophic niches. As open ocean productivity is frequently N limited, characterization of new sources of N will have global impacts.</p>
Microbial associations in zooplankton
largerWorkCitation
project
eng; USA
oceans
Subtropical North Atlantic Ocean
-65.7
-13.3
24.5
27.8
2011-01-28
2011-03-09
Sargasso Sea, Eastern and Western North Atlantic.
0
BCO-DMO catalogue of parameters from Quantitative PCR data from samples collected on the R/V Sarmiento de Gamboa cruise in the subtropical North Atlantic Ocean between January and March 2011 (Microbial associations in zooplankton project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/681467.rdf
Name: number
Units: unitless
Description: Sample ID number
http://lod.bco-dmo.org/id/dataset-parameter/681468.rdf
Name: project
Units: unitless
Description: PI issued project name
http://lod.bco-dmo.org/id/dataset-parameter/681469.rdf
Name: date
Units: unitless
Description: Date of sampling; YYYY/mm/dd
http://lod.bco-dmo.org/id/dataset-parameter/681470.rdf
Name: time
Units: unitless
Description: Time of sampling; HH:MM
http://lod.bco-dmo.org/id/dataset-parameter/681471.rdf
Name: station
Units: unitless
Description: Station where sampling occurred
http://lod.bco-dmo.org/id/dataset-parameter/681472.rdf
Name: lat
Units: decimal degrees
Description: Latitude; N is positive
http://lod.bco-dmo.org/id/dataset-parameter/681473.rdf
Name: lon
Units: decimal degrees
Description: Longitude; E is positive
http://lod.bco-dmo.org/id/dataset-parameter/681474.rdf
Name: size_fraction
Units: microns
Description: Filter size
http://lod.bco-dmo.org/id/dataset-parameter/681475.rdf
Name: UCYN_A
Units: genes per liter
Description: UCYN-A gene copy concentration
http://lod.bco-dmo.org/id/dataset-parameter/681476.rdf
Name: Gamma_24774A11
Units: genes per liter
Description: Gamma 24774A11 gene copy concentration
http://lod.bco-dmo.org/id/dataset-parameter/681477.rdf
Name: group_B
Units: genes per liter
Description: Group B gene copy concentration
http://lod.bco-dmo.org/id/dataset-parameter/681478.rdf
Name: Tricho
Units: genes per liter
Description: Tricho gene copy concentration
http://lod.bco-dmo.org/id/dataset-parameter/681479.rdf
Name: Het1
Units: genes per liter
Description: Het1 gene copy concentration
http://lod.bco-dmo.org/id/dataset-parameter/681480.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: Date/Time (UTC) ISO formatted
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
4880
https://darchive.mblwhoilibrary.org/bitstream/1912/23962/1/dataset-680935_quantitative-pcr-data__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.680935.1
download
onLine
dataset
<p>Samples were collected from Niskin bottles, filtered, and processed for DNA analyses. For detailed methods see Benavides et al. 2016.</p>
Specified by the Principal Investigator(s)
<p>For qPCR methods, see Benavides et al. 2016.</p>
<p><strong>BCO-DMO Data Processing Notes:</strong><br />
-Reformatted column names to comply with BCO-DMO standards<br />
-Added ISO_DateTime_UTC column<br />
-Filled in blank cells with nd<br />
-Reformatted date to YYYY/MM/DD</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PCR
PCR
PI Supplied Instrument Name: PCR PI Supplied Instrument Description:Used to process PCR samples Instrument Name: Thermal Cycler Instrument Short Name:Thermal Cycler Instrument Description: A thermal cycler or "thermocycler" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.
(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)
Cruise: Malaspina_2011
Malaspina_2011
R/V Sarmiento de Gamboa
vessel
Malaspina_2011
Pia Moisander
University of Massachusetts Dartmouth
R/V Sarmiento de Gamboa
vessel