http://lod.bco-dmo.org/id/dataset/723957
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-01-17
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Potential denitrification and N2 fixation from slurry assays from Little Lagoon, Alabama collected from 2012-2013
2018-01-16
publication
2018-01-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-15
publication
https://doi.org/10.1575/1912/bco-dmo.723957.1
Behzad Mortazavi
National Science Foundation
principalInvestigator
William C. Burnett
Florida State University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Mortazavi, B., Burnett, W. (2018) Potential denitrification and N2 fixation from slurry assays from Little Lagoon, Alabama collected from 2012-2013. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-01-16 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.723957.1 [access date]
DNF and Nitrogen fixation data. Dataset Description: <p>Potential denitrification and N2 fixation from slurry assays from Little Lagoon, Alabama.</p> Methods and Sampling: <p>Little Lagoon is a shallow coastal lagoon that is tidally connected to the Gulf of Mexico but has no riverine inputs. The water in the lagoon is replenished solely from precipitation and groundwater inputs primarily on the East end (Su et al. 2012). Because of the rapid development in Baldwin County, a large amount of NO3- enters the Little Lagoon system through SGD (Murgulet &amp; Tick 2008). In this region, there can be rapid changes in the depth to groundwater (Fig. 4.1 inset) and episodic SGD inputs to the lagoon (Su et al.2013). Within the lagoon, three sites were selected (East, Mouth, and West) to represent the gradient that exists across the lagoon from the input of groundwater. Sites were sampled on a near-monthly basis from February 2012 to February 2013.</p>
<p><strong>Potential denitrification and N2 fixation from slurry assays</strong></p>
<p>The top 50 mm of sediment at each site were collected in duplicate with a large core (95 mm ID) and homogenized. Potential denitrification rates were measured following the acetylene inhibition technique (Sørensen 1978) but may underestimate denitrification supported by coupled nitrification-denitrification, as this method inhibits nitrification. To triplicate serum vials, approximately 20 g of sediments and filtered (0.7 micron) site water were added at various treatments (control and N addition (100 μM and 500 μM KNO3 -). Samples were sealed with a butyl rubber stopper, capped and flushed with N2 gas for 10 minutes. After the addition of C2H2 (10% v/v) and a 1-hour incubation, headspace gas samples were injected into evacuated 12 ml Exetainer vials and N2O production was quantified with a Shimadzu GC-2014 with an electron capture detector (GC-ECD) within 24 hours.</p>
<p>Potential N2 fixation rates were measured as ethylene (C2H4) production from acetylene (C2H2) reduction (Welsh et al. 1996) in triplicate from slurry assays containing 20 g of homogenized sediment and filtered (0.7 micron) site water. Rates of N2 fixation by sulfate reducing bacteria (SRB) were determined after the addition of sodium molybdate as a specific inhibitor of the sulfate reduction process (Hardy et al. 1973, Capone 1993). After C2H4 analysis on a Shimadzu gas chromatograph (GC-2014) with flame ionization detection (GC-FID), production rates of C2H4 were converted to potential N2 fixation rates using a C2H2:N2 reduction ratio of 3:1 (Capone 1993).</p>
<p><strong>Additional methodology can be found in:</strong></p>
<p>Bernard, Rebecca &amp; Mortazavi, Behzad &amp; A. Kleinhuizen, Alice. (2015). Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates NO3− reduction pathways in an anthropogenically impacted sub-tropical coastal lagoon. Biogeochemistry. 125. 47-64.&nbsp;<a href="https://link.springer.com/article/10.1007%2Fs10533-015-0111-6" target="_blank">10.1007/s10533-015-0111-6</a>.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0962008 Award URL: http://nsf.gov/awardsearch/showAward?AWD_ID=0962008
completed
Behzad Mortazavi
National Science Foundation
251-861-2141 X2189
University of Alabama, Dauphin Island Sea Laboratory 101 Bienville Blvd
Dauphin Island
AL
36528
bmortazavi@ua.edu
pointOfContact
William C. Burnett
Florida State University
850-644-6703
Department of Earth, Ocean and Atmospheric Sciences Florida State University
Tallahassee
FL
32206
USA
wburnett@fsu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Year
Date
Value_Description
Mouth
Mouth_SE
West
West_SE
East
East_SE
theme
None, User defined
No BCO-DMO term
date
Nitrogen
featureType
BCO-DMO Standard Parameters
LittleLagoon
service
Deployment Activity
Little Lagoon, Alabama
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Groundwater Discharge, Benthic Coupling and Microalgal Community Structure in a Shallow Coastal Lagoon
https://www.bco-dmo.org/project/491318
Groundwater Discharge, Benthic Coupling and Microalgal Community Structure in a Shallow Coastal Lagoon
<p> </p>
<p>This project investigated the link between submarine groundwater discharge (SGD) and microalgal dynamics in Little Lagoon, Alabama. In contrast to most near-shore environments, it is fully accessible; has no riverine inputs; and is large enough to display ecological diversity (c. 14x 0.75 km) yet small enough to be comprehensively sampled on appropriate temporal and spatial scales. The PIs have previously demonstrated that the lagoon is a hot-spot for toxic blooms of the diatom <em>Pseudo-nitzchia spp.</em> that are correlated with discharge from the surficial aquifer. This project assessed variability in SGD, the dependence of benthic nutrient fluxes on microphytobenthos (MPB) abundance and productivity, and the response of the phytoplankton to nutrient enrichment and dilution. The work integrated multiple temporal and spatial scales and demonstrated both the relative importance of SGD vs. benthic recycling as a source of nutrients, and the role of SGD in structuring the microalgal community. (<em>paraphrased from Award abstract</em>)</p>
LittleLagoonGroundwater
largerWorkCitation
project
eng; USA
oceans
Little Lagoon, Alabama
-87.773756
-87.773756
30.241929
30.241929
2012-01-01
2013-12-31
southern Alabama, east of Mobile
0
BCO-DMO catalogue of parameters from Potential denitrification and N2 fixation from slurry assays from Little Lagoon, Alabama collected from 2012-2013
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/724271.rdf
Name: Year
Units: unitless
Description: Year ID that samples were taken
http://lod.bco-dmo.org/id/dataset-parameter/724272.rdf
Name: Date
Units: unitless
Description: Month and day that samples were taken; MMM-DD
http://lod.bco-dmo.org/id/dataset-parameter/724273.rdf
Name: Value_Description
Units: unitless
Description: Description of the measurment taken; description includes relevant units for each sample taken
http://lod.bco-dmo.org/id/dataset-parameter/724274.rdf
Name: Mouth
Units: nanomol of nitrogen per gram per hour
Description: Denitrification and Nitrogen fixation values collected at the site Mouth; location of site is 30.243683, -87.738407
http://lod.bco-dmo.org/id/dataset-parameter/724275.rdf
Name: Mouth_SE
Units: nanomol of nitrogen per gram per hour
Description: Standard error of the values collected at the site Mouth
http://lod.bco-dmo.org/id/dataset-parameter/724276.rdf
Name: West
Units: nanomol of nitrogen per gram per hour
Description: Denitrification and Nitrogen fixation values collected at the site West; location of site is 30.247181, -87.767856
http://lod.bco-dmo.org/id/dataset-parameter/724277.rdf
Name: West_SE
Units: nanomol of nitrogen per gram per hour
Description: Standard error of the values collected at the site West
http://lod.bco-dmo.org/id/dataset-parameter/724278.rdf
Name: East
Units: nanomol of nitrogen per gram per hour
Description: Denitrification and Nitrogen fixation values collected at the site East; location of site is 30.253347, -87.724729
http://lod.bco-dmo.org/id/dataset-parameter/724279.rdf
Name: East_SE
Units: nanomol of nitrogen per gram per hour
Description: Standard error of the values collected at the site East
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
580
https://darchive.mblwhoilibrary.org/bitstream/1912/23834/1/dataset-723957_denitrification-and-nitrogen-fixation__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.723957.1
download
onLine
dataset
<p>Little Lagoon is a shallow coastal lagoon that is tidally connected to the Gulf of Mexico but has no riverine inputs. The water in the lagoon is replenished solely from precipitation and groundwater inputs primarily on the East end (Su et al. 2012). Because of the rapid development in Baldwin County, a large amount of NO3- enters the Little Lagoon system through SGD (Murgulet &amp; Tick 2008). In this region, there can be rapid changes in the depth to groundwater (Fig. 4.1 inset) and episodic SGD inputs to the lagoon (Su et al.2013). Within the lagoon, three sites were selected (East, Mouth, and West) to represent the gradient that exists across the lagoon from the input of groundwater. Sites were sampled on a near-monthly basis from February 2012 to February 2013.</p>
<p><strong>Potential denitrification and N2 fixation from slurry assays</strong></p>
<p>The top 50 mm of sediment at each site were collected in duplicate with a large core (95 mm ID) and homogenized. Potential denitrification rates were measured following the acetylene inhibition technique (Sørensen 1978) but may underestimate denitrification supported by coupled nitrification-denitrification, as this method inhibits nitrification. To triplicate serum vials, approximately 20 g of sediments and filtered (0.7 micron) site water were added at various treatments (control and N addition (100 μM and 500 μM KNO3 -). Samples were sealed with a butyl rubber stopper, capped and flushed with N2 gas for 10 minutes. After the addition of C2H2 (10% v/v) and a 1-hour incubation, headspace gas samples were injected into evacuated 12 ml Exetainer vials and N2O production was quantified with a Shimadzu GC-2014 with an electron capture detector (GC-ECD) within 24 hours.</p>
<p>Potential N2 fixation rates were measured as ethylene (C2H4) production from acetylene (C2H2) reduction (Welsh et al. 1996) in triplicate from slurry assays containing 20 g of homogenized sediment and filtered (0.7 micron) site water. Rates of N2 fixation by sulfate reducing bacteria (SRB) were determined after the addition of sodium molybdate as a specific inhibitor of the sulfate reduction process (Hardy et al. 1973, Capone 1993). After C2H4 analysis on a Shimadzu gas chromatograph (GC-2014) with flame ionization detection (GC-FID), production rates of C2H4 were converted to potential N2 fixation rates using a C2H2:N2 reduction ratio of 3:1 (Capone 1993).</p>
<p><strong>Additional methodology can be found in:</strong></p>
<p>Bernard, Rebecca &amp; Mortazavi, Behzad &amp; A. Kleinhuizen, Alice. (2015). Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates NO3− reduction pathways in an anthropogenically impacted sub-tropical coastal lagoon. Biogeochemistry. 125. 47-64.&nbsp;<a href="https://link.springer.com/article/10.1007%2Fs10533-015-0111-6" target="_blank">10.1007/s10533-015-0111-6</a>.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Data were flagged as below detection limits if no measurable rates were returned after calculations. See equations in methodology section of:</p>
<p>Bernard, Rebecca &amp; Mortazavi, Behzad &amp; A. Kleinhuizen, Alice. (2015). Dissimilatory nitrate reduction to ammonium (DNRA) seasonally dominates NO3− reduction pathways in an anthropogenically impacted sub-tropical coastal lagoon. Biogeochemistry. 125. 47-64.&nbsp;<a href="https://link.springer.com/article/10.1007%2Fs10533-015-0111-6" target="_blank">10.1007/s10533-015-0111-6</a>.&nbsp;</p>
<p><strong>Statistical Analysis</strong></p>
<p>To test the seasonal flux variability between sites in Little Lagoon, two-way ANOVAs with site and date as independent variables were performed. When data could not be transformed to meet ANOVA assumptions, Wilcoxon/Kruskal-Wallis nonparametric tests were used. When significant differences occurred, Tukey HSD or Steel-Dwass post hoc tests were used to determine significant interactions. A Principal component analysis (PCA) was conducted on all biogeochemical parameters to identify underlying multivariate components that may be influencing N fluxes. Spearman’s rho correlation analysis was used to examine the relationship between the principal components and fluxes. Statistical significance of the data set was determined at α=0.05 and error is reported as standard error. All statistical analyses were performed in SAS JMP 10 (SAS Institute Inc.).</p>
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>- Data reorganized into one table under one set of column names<br />
- Units removed from column names<br />
- Column names reformatted to meet BCO-DMO standards<br />
- Information captured in original column names entered under column Value_Description<br />
- Created column Year to describe to capture the metadata in the file name</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Deployment: LittleLagoon
LittleLagoon
SmallBoat_FSU
stationary vessel
LittleLagoon
William C. Burnett
Florida State University
SmallBoat_FSU
stationary vessel