http://lod.bco-dmo.org/id/dataset/732177
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-03-27
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
2018-03-22
publication
2018-03-22
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-05
publication
https://doi.org/10.1575/1912/bco-dmo.732177.1
Janet E. Kubler
California State University Northridge
principalInvestigator
Steve Dudgeon
California State University Northridge
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Kubler, J., Dudgeon, S. (2018) Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-03-22 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.732177.1 [access date]
Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability Dataset Description: <p>This dataset reports the carbonate chemistry along with temperature, salinity and pH&nbsp;of Ulva lactuca&nbsp;grown in closed culture pots at varying pCO2 levels.</p>
<p><strong>Related Datasets:</strong><br />
<a href="https://www.bco-dmo.org/dataset/732438">Ulva: Chl a</a>: Chlorophyll a per unit biomass in Ulva lactuca under ocean acidification (OA) conditions (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732564">Ulva: CHN and stable isotopes</a>: Stable isotope ratios and mass of carbon and nitrogen in Ulva cells under ocean acidification conditions (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732600">Ulva: Growth</a>: Growth rates of Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732493">Ulva: pH and temperature time-series</a>: Time-series at 10 minute sampling interval of pH and temperature in Ulva culture pots (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732464">Ulva: pH Drift</a>: Carbonate chemistry over a time course with Ulva in pH drift experiments (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732625">Ulva: Photosynthesis and respiration</a>: Rates of photosynthesis and respiration by Ulva exposed to different average and variability of pCO2 (Seaweed OA Resilience project)<br />
<a href="https://www.bco-dmo.org/dataset/732587">Ulva: seawater delta13C</a>: Stable isotope ratio and concentration of carbon in seawater from Ulva OA experiments (Seaweed OA Resilience project)</p> Methods and Sampling: <p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at 15° C under saturating illumination of ~550 µmoles photons/m^2/s on a 12:12 L:D cycle. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.</p>
<p>Carbonate chemistry parameters were measured by sampling pH and total alkalinity (TA) of water samples. Seawater samples from each culture pot during all 3 trials were collected 2-3 times per week in 50 ml Falcon tubes to monitor the average and variability of experimental treatment conditions. Water samples were measured usually within 1-2 hours of sample collection. pH was determined using the m-cresol indicator dye method in a spectrophotometer (Dickson et al. 2007). TA samples were analyzed by potentiometric titration coupled to a pH electrode calibrated using certified reference material (CRM) from the Dickson laboratory at Scripps Oceanographic Institute and the pH electrode calibrated using TRIS buffer (Dickson et al. 2007). TA and carbonate parameters were calculated from potentiometric titration data and spectrophotometric pH data.</p>
<p>Carbonate chemistry parameters (CO2 concentration, CO2 partial pressure, CO2 fugacity, HCO3, CO3, DIC, Omega Aragonite, Omega Calcite) and total scale pH at in situ temperature were calculated using the seacarb package (V3.0.14) in R (Lavigne et al. 2011).</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1316198 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1316198
completed
Janet E. Kubler
California State University Northridge
(818) 677-7322
Dept. of Biology, CSU-Northridge 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
janet.kubler@csun.edu
pointOfContact
Steve Dudgeon
California State University Northridge
(818) 677-7322
Dept. of Biology, CSU-Northridge 18111 Nordhoff Street
Northridge
CA
91330-8303
USA
steve.dudgeon@csun.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Culture_Pot_ID
Exp_Trial
Date
days_diff
pCO2weights
wt_avg_pCO2
wt_sd_pCO2
Sal_in
Temp_in
pH25
pHin
CO2
pCO2
fCO2
HCO3
CO3
DIC
ALK
OmegaAragonite
OmegaCalcite
Shimadzu UV-2450 UV-visible spectrophotometer
Thermo Fisher Orion Star 329
Mettler Toledo T50 equipped with Rondolino
Qubit Systems Mass Flow Controllers (MFC)
Temperature control chiller: Aqua Logic Cyclone Chiller
theme
None, User defined
sample identification
replicate
date_local
days
No BCO-DMO term
Partial pressure of CO2
salinity
water temperature
pH
total Carbon Dioxide in seawater
fugacity of CO2
bicarbonate concentration [HCO3]-
Carbonate concentration [CO3]2-
dissolved inorganic Carbon
total alkalinity (TA)
Aragonite Saturation State
Calcite Saturation State
featureType
BCO-DMO Standard Parameters
UV Spectrophotometer-Shimadzu
Multi Parameter Bench Meter
Automatic titrator
Mass Flow Controller
Aquarium chiller
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:NSF 10-530, FY 2010-FY2011NSF 12-500, FY 2012NSF 12-600, FY 2013NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
https://www.bco-dmo.org/project/2275
Ocean Acidification: Scope for Resilience to Ocean Acidification in Macroalgae
<p>Benthic macroalgae contribute to intensely productive near shore ecosystems and little is known about the potential effects of ocean acidification on non-calcifying macroalgae. Kübler and Dudgeon will test hypotheses about two macroalgae, <em>Ulva</em> spp. and <em>Plocamium cartilagineum</em>, which, for different reasons, are hypothesized to be more productive and undergo ecological expansions under predicted changes in ocean chemistry. They have designed laboratory culture-based experiments to quantify the scope for response to ocean acidification in <em>Plocamium</em>, which relies solely on diffusive uptake of CO2, and populations of<em> Ulva</em> spp., which have an inducible concentrating mechanism (CCM). The investigators will culture these algae in media equilibrated at 8 different pCO2 levels ranging from 380 to 940 ppm to address three key hypotheses. The first is that macroalgae (such as Plocamium cartilagineum) that are not able to acquire inorganic carbon in changed form will benefit, in terms of photosynthetic and growth rates, from ocean acidification. There is little existing data to support this common assumption. The second hypothesis is that enhanced growth of Ulva sp. under OA will result from the energetic savings from down regulating the CCM, rather than from enhanced photosynthesis per se. Their approach will detect existing genetic variation for adaptive plasticity. The third key hypothesis to be addressed in short-term culture experiments is that there will be a significant interaction between ocean acidification and nitrogen limited growth of<em> Ulva</em> spp., which are indicator species of eutrophication. Kübler and Dudgeon will be able to quantify the individual effects of ocean acidification and nitrogenous nutrient addition on <em>Ulva</em> spp. and also, the synergistic effects, which will inevitably apply in many highly productive, shallow coastal areas. The three hypotheses being addressed have been broadly identified as urgent needs in our growing understanding of the impacts of ocean acidification.</p>
Seaweed OA Resilience
largerWorkCitation
project
eng; USA
biota
oceans
-119
-118
33
34
2015-05-11
2015-07-27
Temperate coastal waters of the USA (30 - 45 N latitude, -66 to -88 W and -117 to -125 W longitude)
0
BCO-DMO catalogue of parameters from Carbonate chemistry of Ulva lactuca culture pots testing the effects of pCO2 variability (Seaweed OA Resilience project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/732418.rdf
Name: Culture_Pot_ID
Units: unitless
Description: Identification number of culture
http://lod.bco-dmo.org/id/dataset-parameter/732419.rdf
Name: Exp_Trial
Units: unitless
Description: Trial consisting of a range of average of pCO2 levels crossed with either or low or high variability around the average pCO2 effect
http://lod.bco-dmo.org/id/dataset-parameter/732420.rdf
Name: Date
Units: unitless
Description: Date formatted as yyyy-mm-dd
http://lod.bco-dmo.org/id/dataset-parameter/732421.rdf
Name: days_diff
Units: days
Description: Number of days between present and subsequent seawater samples to measure carbonate chemistry parameters
http://lod.bco-dmo.org/id/dataset-parameter/732422.rdf
Name: pCO2weights
Units: microatmosphere days (µatm.days)
Description: Product of days_diff and pCO2: used to generated weighted estimates of the average and standard deviation carbonate chemistry parameters for each culture pot in each experimental trial
http://lod.bco-dmo.org/id/dataset-parameter/732423.rdf
Name: wt_avg_pCO2
Units: microatmospheres (µatm)
Description: Weighted average of pCO2 partial pressure in seawater tanks
http://lod.bco-dmo.org/id/dataset-parameter/732424.rdf
Name: wt_sd_pCO2
Units: microatmospheres (µatm)
Description: Weighted standard deviation of pCO2 partial pressure in seawater tanks
http://lod.bco-dmo.org/id/dataset-parameter/732425.rdf
Name: Sal_in
Units: parts per thousand (ppt)
Description: Salinity in situ
http://lod.bco-dmo.org/id/dataset-parameter/732426.rdf
Name: Temp_in
Units: degrees Celsius
Description: Temperature in situ
http://lod.bco-dmo.org/id/dataset-parameter/732427.rdf
Name: pH25
Units: unitless
Description: pH in Total scale at 25 deg C
http://lod.bco-dmo.org/id/dataset-parameter/732428.rdf
Name: pHin
Units: unitless
Description: pH-Total scale in situ temperature
http://lod.bco-dmo.org/id/dataset-parameter/732429.rdf
Name: CO2
Units: micromoles/kilogram
Description: Carbon dioxide concentration
http://lod.bco-dmo.org/id/dataset-parameter/732430.rdf
Name: pCO2
Units: microatmospheres (µatm)
Description: CO2 partial pressure
http://lod.bco-dmo.org/id/dataset-parameter/732431.rdf
Name: fCO2
Units: microatmospheres (µatm)
Description: CO2 fugacity
http://lod.bco-dmo.org/id/dataset-parameter/732432.rdf
Name: HCO3
Units: micromoles/kilogram
Description: Bicarbonate ion concentration
http://lod.bco-dmo.org/id/dataset-parameter/732433.rdf
Name: CO3
Units: micromoles/kilogram
Description: Carbonate ion concentration
http://lod.bco-dmo.org/id/dataset-parameter/732434.rdf
Name: DIC
Units: micromoles/kilogram
Description: Total dissolved inorganic carbon
http://lod.bco-dmo.org/id/dataset-parameter/732435.rdf
Name: ALK
Units: micromoles/kilogram
Description: Total Alkalinity measured
http://lod.bco-dmo.org/id/dataset-parameter/732436.rdf
Name: OmegaAragonite
Units: unitless
Description: Aragonite saturation state
http://lod.bco-dmo.org/id/dataset-parameter/732437.rdf
Name: OmegaCalcite
Units: unitless
Description: Calcite Saturation State
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
26582
https://darchive.mblwhoilibrary.org/bitstream/1912/24202/1/dataset-732177_ulva-carbonate-chemistry-pco2__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.732177.1
download
onLine
dataset
<p>Culture pots were placed in large thermally insulated coolers in a temperature-controlled water bath at 15° C under saturating illumination of ~550 µmoles photons/m^2/s on a 12:12 L:D cycle. pCO2 treatments were supplied to closed culture pots by use of a gas mixing system combining Nitrogen, Oxygen and Carbon Dioxide to specific CO2 partial pressures, 20.9% oxygen and the balance being Nitrogen.</p>
<p>Carbonate chemistry parameters were measured by sampling pH and total alkalinity (TA) of water samples. Seawater samples from each culture pot during all 3 trials were collected 2-3 times per week in 50 ml Falcon tubes to monitor the average and variability of experimental treatment conditions. Water samples were measured usually within 1-2 hours of sample collection. pH was determined using the m-cresol indicator dye method in a spectrophotometer (Dickson et al. 2007). TA samples were analyzed by potentiometric titration coupled to a pH electrode calibrated using certified reference material (CRM) from the Dickson laboratory at Scripps Oceanographic Institute and the pH electrode calibrated using TRIS buffer (Dickson et al. 2007). TA and carbonate parameters were calculated from potentiometric titration data and spectrophotometric pH data.</p>
<p>Carbonate chemistry parameters (CO2 concentration, CO2 partial pressure, CO2 fugacity, HCO3, CO3, DIC, Omega Aragonite, Omega Calcite) and total scale pH at in situ temperature were calculated using the seacarb package (V3.0.14) in R (Lavigne et al. 2011).</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added a conventional header with dataset&nbsp;name and description, PI names, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- reformatted date from m/d/yyyy to yyyy-mm-dd</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Shimadzu UV-2450 UV-visible spectrophotometer
Shimadzu UV-2450 UV-visible spectrophotometer
PI Supplied Instrument Name: Shimadzu UV-2450 UV-visible spectrophotometer PI Supplied Instrument Description:Used to measure pH total scale at 25C. Instrument Name: UV Spectrophotometer-Shimadzu Instrument Short Name:UV Spectrophotometer-Shimadzu Instrument Description: The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Thermo Fisher Orion Star 329
Thermo Fisher Orion Star 329
PI Supplied Instrument Name: Thermo Fisher Orion Star 329 PI Supplied Instrument Description:Used to measure pH, temperature and dissolved oxygen. Instrument Name: Multi Parameter Bench Meter Instrument Short Name:Multi Parameter Bench Meter Instrument Description: An analytical instrument that can measure multiple parameters, such as pH, EC, TDS, DO and Temperature with one device.
Mettler Toledo T50 equipped with Rondolino
Mettler Toledo T50 equipped with Rondolino
PI Supplied Instrument Name: Mettler Toledo T50 equipped with Rondolino PI Supplied Instrument Description:Used to measure total alkalinity. Instrument Name: Automatic titrator Instrument Short Name:Automatic titrator Instrument Description: Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Qubit Systems Mass Flow Controllers (MFC)
Qubit Systems Mass Flow Controllers (MFC)
PI Supplied Instrument Name: Qubit Systems Mass Flow Controllers (MFC) PI Supplied Instrument Description:Settings: Nitrogen, 1 L/min; Oxygen, 250 ml/min; CO2, 2 ml/min. Instrument Name: Mass Flow Controller Instrument Short Name:MFC Instrument Description: Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
Temperature control chiller: Aqua Logic Cyclone Chiller
Temperature control chiller: Aqua Logic Cyclone Chiller
PI Supplied Instrument Name: Temperature control chiller: Aqua Logic Cyclone Chiller Instrument Name: Aquarium chiller Instrument Short Name:Aquarium chiller Instrument Description: Immersible or in-line liquid cooling device, usually with temperature control.