http://lod.bco-dmo.org/id/dataset/778021
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-09-30
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Analysis of dissolved 15N2 gas standards by Membrane Inlet Mass Spectrometry
2019-10-02
publication
2019-10-02
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-01-06
publication
https://doi.org/10.1575/1912/bco-dmo.778021.1
Julie Granger
University of Connecticut
principalInvestigator
Annie Bourbonnais
University of Massachusetts Dartmouth
principalInvestigator
Samuel Wilson
University of Hawaii
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Granger, J., Bourbonnais, A., Wilson, S. (2019) Analysis of dissolved 15N2 gas standards by Membrane Inlet Mass Spectrometry. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-10-02 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.778021.1 [access date]
Analysis of dissolved 15N2 gas standards by Membrane Inlet Mass Spectrometry Dataset Description: <p>Analysis of dissolved 15N2 gas standards by Membrane Inlet Mass Spectrometry</p> Methods and Sampling: <p>We determined that the most reliable way to prepare dissolved 15N2 gas standards involves equilibration of the liquid phase with a headspace, rather than injection of a 15N2 gas aliquot into a sealed bottle without a headspace. Standards were prepared in a room with a relatively constant ambient temperature. Serial aliquots of 15N2 gas were each injected into the headspace of crimp-sealed 120 mL serum bottles containing 80 mL of air-equilibrated seawater (at room temperature) and a stir bar with an unpressurized air headspace. The vials were equilibrated for 72 hours on a stir plate (at low rpm) prior to analysis on the MIMS or IRMS.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1732246 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1732246
completed
Julie Granger
University of Connecticut
860-405-9094
Department of Marine Sciences 1080 Shenecossett Road
Groton
CT
06340
USA
julie.granger@uconn.edu
pointOfContact
Annie Bourbonnais
University of Massachusetts Dartmouth
abourbonnais@seoe.sc.edu
pointOfContact
Samuel Wilson
University of Hawaii
808-956-0573
1950 East-West Road C-MORE Hale
Honlulu
HI
96822
USA
stwilson@hawaii.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Bottle_vol
Sample
Injection_15N2
Expected_15N_At_pcnt
Time_of_analysis
m_z_28
m_z_29
m_z_30
m_z_32
m_z_40
N2_Ar
ratio_28_29
ratio_28_30
At_15N_pcnt
At_15N_pcnt_avg
Isotope Ratio Mass Spectrometer
Membrane Inlet Mass Spectrometer
theme
None, User defined
No BCO-DMO term
time of day
featureType
BCO-DMO Standard Parameters
Isotope-ratio Mass Spectrometer
Membrane Inlet Mass Spectrometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean
https://www.bco-dmo.org/project/772534
EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean
<p>NSF Award Abstract:<br />
The availability of nitrogen is required to support the growth and production of organisms living in the surface of our global ocean. This element can be scarce. To alleviate this scarcity, a special class of bacteria and archaea, called nitrogen fixers, can derive the nitrogen needed for growth from nitrogen gas. This project would carefully examine one specific method for measuring nitrogen fixation that has been used recently to suggest the occurrence of small amounts of nitrogen fixation in subsurface ocean waters. If these reports are verified, then a revision of our understanding of the marine nitrogen cycle may be needed. The Ocean Carbon and Biogeochemistry program will be used as a platform to develop community consensus for best practices in nitrogen fixation measurements and detection of diversity, activity, and abundances of the organisms responsible. In addition, a session will be organized in a future national/international conference to communicate with the broader scientific community while developing these best practices.</p>
<p>The goal of this study is to conduct a thorough examination of potential experimental and analytical errors inherent to the 15N2-tracer nitrogen fixation method, in tandem with comprehensive molecular measurements, in mesopelagic ocean waters. Samples will be collected and experimental work conducted on a cruise transect in the North Atlantic Ocean, followed by analytical work in the laboratory. The specific aims of this study are to (1) determine the minimum quantifiable rates of 15N2 fixation based on incubations of mesopelagic waters via characterization of sources of experimental and analytical error, and (2) seek evidence of presence and expression of nitrogen fixation genes via comprehensive molecular approaches on corresponding samples. The range of detectable rates and diazotroph activity from the measurements made in this study will be informative for the understanding of the importance of nitrogen fixation in the oceanic nitrogen budget.</p>
EAGER NitFix
largerWorkCitation
project
eng; USA
oceans
2019-10-02
North Atlantic Ocean, Pacific Ocean
0
BCO-DMO catalogue of parameters from Analysis of dissolved 15N2 gas standards by Membrane Inlet Mass Spectrometry
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/778031.rdf
Name: Bottle_vol
Units: mililiters (mL)
Description: volume of the bottle
http://lod.bco-dmo.org/id/dataset-parameter/778032.rdf
Name: Sample
Units: unitless
Description: type of sample
http://lod.bco-dmo.org/id/dataset-parameter/778033.rdf
Name: Injection_15N2
Units: mililiters (mL)
Description: amount of Injection of 15N2
http://lod.bco-dmo.org/id/dataset-parameter/778034.rdf
Name: Expected_15N_At_pcnt
Units: unitless
Description: expected 15N At %
http://lod.bco-dmo.org/id/dataset-parameter/778035.rdf
Name: Time_of_analysis
Units: unitless
Description: time of analysis in 24 hour format
http://lod.bco-dmo.org/id/dataset-parameter/778036.rdf
Name: m_z_28
Units: unitless
Description: mass-to-charge
http://lod.bco-dmo.org/id/dataset-parameter/778037.rdf
Name: m_z_29
Units: unitless
Description: mass-to-charge
http://lod.bco-dmo.org/id/dataset-parameter/778038.rdf
Name: m_z_30
Units: unitless
Description: mass-to-charge
http://lod.bco-dmo.org/id/dataset-parameter/778039.rdf
Name: m_z_32
Units: unitless
Description: mass-to-charge
http://lod.bco-dmo.org/id/dataset-parameter/778040.rdf
Name: m_z_40
Units: unitless
Description: mass-to-charge
http://lod.bco-dmo.org/id/dataset-parameter/778041.rdf
Name: N2_Ar
Units: unitless
Description: N2/Ar ratio
http://lod.bco-dmo.org/id/dataset-parameter/778042.rdf
Name: ratio_28_29
Units: unitless
Description: 28/29 ratio
http://lod.bco-dmo.org/id/dataset-parameter/778043.rdf
Name: ratio_28_30
Units: unitless
Description: 28/30 ratio
http://lod.bco-dmo.org/id/dataset-parameter/778044.rdf
Name: At_15N_pcnt
Units: unitless
Description: 15N At%
http://lod.bco-dmo.org/id/dataset-parameter/778045.rdf
Name: At_15N_pcnt_avg
Units: unitless
Description: 15N At% average
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
8766
https://darchive.mblwhoilibrary.org/bitstream/1912/25116/1/dataset-778021_dis15n2gas__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.778021.1
download
onLine
dataset
<p>We determined that the most reliable way to prepare dissolved 15N2 gas standards involves equilibration of the liquid phase with a headspace, rather than injection of a 15N2 gas aliquot into a sealed bottle without a headspace. Standards were prepared in a room with a relatively constant ambient temperature. Serial aliquots of 15N2 gas were each injected into the headspace of crimp-sealed 120 mL serum bottles containing 80 mL of air-equilibrated seawater (at room temperature) and a stir bar with an unpressurized air headspace. The vials were equilibrated for 72 hours on a stir plate (at low rpm) prior to analysis on the MIMS or IRMS.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing Notes:<br />
- table was extracted from original spreadsheet.<br />
-&nbsp;added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Isotope Ratio Mass Spectrometer
Isotope Ratio Mass Spectrometer
PI Supplied Instrument Name: Isotope Ratio Mass Spectrometer PI Supplied Instrument Description:continuous flow Delta V Isotope Ratio Mass Spectrometer (Smith et al. 2015), and continuous flow-GV Isoprime IRMS (Charoenpong et al., 2014) Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Membrane Inlet Mass Spectrometer
Membrane Inlet Mass Spectrometer
PI Supplied Instrument Name: Membrane Inlet Mass Spectrometer PI Supplied Instrument Description:Membrane Inlet Mass Spectrometer (Bay Instruments) Instrument Name: Membrane Inlet Mass Spectrometer Instrument Short Name:MIMS Instrument Description: Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.