bcodmo_dataset_988643_v1
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UTF8
dataset
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Adam Shepherd
BCO-DMO
508-289-2772
266 Woods Hole Rd. MS#36
Woods Hole
MA
02543
USA
erddap@bco-dmo.org
pointOfContact
2026-08-30
ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
3
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[EN683 FLA-Bead Data and ULake] - Detection of polysaccharide enzymatic hydrolysis using a novel detection method from waters taken aboard the R/V Endeavor in the Western North Atlantic (EN683 in May and June, 2022), and from a freshwater lake in North Carolina in 2025 (Substrate structural complexity and abundance control distinct mechanisms of microbially-driven carbon cycling in the ocean)
2026-08-30
creation
erddap.bco-dmo.org
bcodmo_dataset_988643_v1
BCO-DMO
info@bco-dmo.org
https://osprey.bco-dmo.org/dataset/988643
information
web browser
Background Information
Background information from the source
information
originator
A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). However, the current method is extremely work-intensive, and results are usually acquired months after initial sampling. Having a proxy to determine real-time enzymatic activity would be useful for scientists doing field work because it would provide a guide as to when and where optimal conditions are located to conduct water column sampling. In an effort to improve field detection techniques, a novel and relatively rapid detection method was developed to detect the hydrolysis of fluorescently-labeled laminarin that is linked to epoxy-activated-agarose beads. Laminarin was used because it is commonly found throughout the surface water of the ocean (Becker et al. 2020). Our goal is to detect potential enzymatic activity within 48 hours using minimal lab equipment while maintaining an accuracy similar to the current standard method used in previous research. Furthermore, we hope the method will provide real-time information for scientists in the field.
This dataset includes the detection of polysaccharide enzymatic hydrolysis using a novel and relatively rapid detection method from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022, and from a local freshwater lake.
BCO-DMO
info@bco-dmo.org
https://osprey.bco-dmo.org/dataset/988643
information
web browser
Background Information
Background information from the source
information
pointOfContact
earth science
atmosphere
ocean
biosphere
biology
environment
theme
BCO-DMO
project
latitude
longitude
depth
theme
CF
The data may be used and redistributed for free but is not intended
for legal use, since it may contain inaccuracies. Neither the data
Contributor, ERD, NOAA, nor the United States Government, nor any
of their employees or contractors, makes any warranty, express or
implied, including warranties of merchantability and fitness for a
particular purpose, or assumes any legal liability for the accuracy,
completeness, or usefulness, of this information.
BCO-DMO
largerWorkCitation
project
eng
geoscientificInformation
1
-79.0935
-53.8678
33.74342
42.8701
-4092.0
-0.2
[EN683 FLA-Bead Data and ULake] - Detection of polysaccharide enzymatic hydrolysis using a novel detection method from waters taken aboard the R/V Endeavor in the Western North Atlantic (EN683 in May and June, 2022), and from a freshwater lake in North Carolina in 2025 (Substrate structural complexity and abundance control distinct mechanisms of microbially-driven carbon cycling in the ocean)
2026-08-30
creation
BCO-DMO
info@bco-dmo.org
https://osprey.bco-dmo.org/dataset/988643
information
web browser
Background Information
Background information from the source
information
originator
A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). However, the current method is extremely work-intensive, and results are usually acquired months after initial sampling. Having a proxy to determine real-time enzymatic activity would be useful for scientists doing field work because it would provide a guide as to when and where optimal conditions are located to conduct water column sampling. In an effort to improve field detection techniques, a novel and relatively rapid detection method was developed to detect the hydrolysis of fluorescently-labeled laminarin that is linked to epoxy-activated-agarose beads. Laminarin was used because it is commonly found throughout the surface water of the ocean (Becker et al. 2020). Our goal is to detect potential enzymatic activity within 48 hours using minimal lab equipment while maintaining an accuracy similar to the current standard method used in previous research. Furthermore, we hope the method will provide real-time information for scientists in the field.
This dataset includes the detection of polysaccharide enzymatic hydrolysis using a novel and relatively rapid detection method from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022, and from a local freshwater lake.
ERDDAP tabledap
1
-79.0935
-53.8678
33.74342
42.8701
-4092.0
-0.2
tight
ERDDAPtabledapDatasetQueryAndAccess
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1
ERDDAP:tabledap
ERDDAP-tabledap
ERDDAP's tabledap service (a flavor of OPeNDAP) for tabular (sequence) data. Add different extensions (e.g., .html, .graph, .das, .dds) to the base URL for different purposes.
download
[EN683 FLA-Bead Data and ULake] - Detection of polysaccharide enzymatic hydrolysis using a novel detection method from waters taken aboard the R/V Endeavor in the Western North Atlantic (EN683 in May and June, 2022), and from a freshwater lake in North Carolina in 2025 (Substrate structural complexity and abundance control distinct mechanisms of microbially-driven carbon cycling in the ocean)
2026-08-30
creation
BCO-DMO
info@bco-dmo.org
https://osprey.bco-dmo.org/dataset/988643
information
web browser
Background Information
Background information from the source
information
originator
A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). However, the current method is extremely work-intensive, and results are usually acquired months after initial sampling. Having a proxy to determine real-time enzymatic activity would be useful for scientists doing field work because it would provide a guide as to when and where optimal conditions are located to conduct water column sampling. In an effort to improve field detection techniques, a novel and relatively rapid detection method was developed to detect the hydrolysis of fluorescently-labeled laminarin that is linked to epoxy-activated-agarose beads. Laminarin was used because it is commonly found throughout the surface water of the ocean (Becker et al. 2020). Our goal is to detect potential enzymatic activity within 48 hours using minimal lab equipment while maintaining an accuracy similar to the current standard method used in previous research. Furthermore, we hope the method will provide real-time information for scientists in the field.
This dataset includes the detection of polysaccharide enzymatic hydrolysis using a novel and relatively rapid detection method from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022, and from a local freshwater lake.
OPeNDAP
1
-79.0935
-53.8678
33.74342
42.8701
-4092.0
-0.2
tight
OPeNDAPDatasetQueryAndAccess
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1
OPeNDAP:OPeNDAP
OPeNDAP
An OPeNDAP service for tabular (sequence) data. Add different extensions (e.g., .html, .das, .dds) to the base URL for different purposes.
download
physicalMeasurement
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Adam Shepherd
BCO-DMO
508-289-2772
266 Woods Hole Rd. MS#36
Woods Hole
MA
02543
USA
erddap@bco-dmo.org
distributor
OPeNDAP
DAP/2.0
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html
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Data Subset Form
ERDDAP's version of the OPeNDAP .html web page for this dataset. Specify a subset of the dataset and download the data via OPeNDAP or in many different file types.
download
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph
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Make-A-Graph Form
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mapDigital
This record was created from dataset metadata by ERDDAP Version 2.22