[Nereocystis geographic patterns in physiology and microbiome] - Nereocystis luetkeana geographic patterns in physiology and microbiome from samples collected from 2019 to 2022 at nine sites spanning more than 200 kilometers in Washington state (The role of microbes in driving productivity and carbon fixation in seaweeds)

Project: BCO-DMO Name: BCO-DMO Email: info@bco-dmo.org Institution: BCO-DMO InfoURL: https://osprey.bco-dmo.org/dataset/1000920 Source URL: (local files) [Nereocystis geographic patterns in physiology and microbiome] - Nereocystis luetkeana geographic patterns in physiology and microbiome from samples collected from 2019 to 2022 at nine sites spanning more than 200 kilometers in Washington state (The role of microbes in driving productivity and carbon fixation in seaweeds) Unknown tabular digital data Woods Hole, MA, USA ERDDAP, version 2.22, at BCO-DMO https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_1000920_v1.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_1000920_v1.graph BCO-DMO Canopy kelp are foundational species in coastal ecosystems and host diverse bacterial communities. We used these data to test the association between bull kelp (Nereocystis luetkeana) host traits, blade-associated bacterial taxa and seawater environmental features across nine sites spanning more than 200 kilometers in Washington state. The data collection spans the outer coast site Tatoosh Island, at the entrance of the Strait of Juan de Fuca. Data collection extended eastward to include sites within the Strait of Juan de Fuca and central and southern Puget Sound. Kelp traits and seawater features that were measured are contained within this dataset and include carbon fixation and DOC release, morphometrics of kelp, and nutrient content. Seawater features that were measured were nutrients, sea surface temperature, and salinity in the surrounding water column. Microbial community data based on 16S rRNA amplicon sequencing of kelp blades across all sites are archived at the National Center for Biotechnology Information (NCBI) under BioProject number PRJNA1301461. Bacterial taxa showed differentiation among sites, and blade-associated bacterial densities were higher at the outer coast site compared with the most inland site. Yet, 11 bacterial genera were present in at least 80% of the samples; these taxa probably serve as core members of the N. luetkeana microbiome and show both positive and negative correlations with host health and environmental features. The data indicated strong interrelationships between kelp traits, seawater features and bacterial community composition with implications for the health of this highly productive foundational species in coastal ecosystems. This dataset supports the 2025 Pfister, et al. paper published in Royal Society Open Science (DOI: 10.1098/rsos.250637). Unknown https://osprey.bco-dmo.org/dataset/1000920 Unknown Unknown ground condition Complete As needed -124.7338 -122.3983 48.39369 47.16652 Uncontrolled earth science atmosphere ocean biosphere biology environment Uncontrolled latitude longitude None. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO BCO-DMO Unknown Mailing and Physical Address

Unknown

Unknown Unknown Unknown Unknown Unknown info@bco-dmo.org https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_1000920_v1.graph Web page to make a customized map or graph of the data HTML Unknown Unknown Unknown Unknown Unknown Unknown Point Point BCO-DMO Adam Shepherd Technical Director Mailing and Physical Address

266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org ERDDAP, version 2.22: get metadata; download data; make graphs and maps. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. .asc 1 View OPeNDAP-style ISO-8859-1 comma-separated text. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#ASCII_Service https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_1000920_v1.html .csv 1 Download a ISO-8859-1 comma-separated text table (line 1: names; line 2: units; ISO 8601 times). https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_1000920_v1.html .csvp 1 Download a ISO-8859-1 .csv file with line 1: name (units). 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266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org Content Standard for Digital Geospatial Metadata FGDC-STD-012-2002 universal time None. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information.