http://lod.bco-dmo.org/id/dataset/664013
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2016-11-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Light-dark calcification rates of Pleurochrysis carterae analyzed at Bigelow Laboratory in 2013 (OA Copes Coccoliths project)
2016-11-04
publication
2016-11-04
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-04-18
publication
https://doi.org/10.1575/1912/bco-dmo.664013.1
William M. Balch
Bigelow Laboratory for Ocean Sciences
principalInvestigator
David Fields
Bigelow Laboratory for Ocean Sciences
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Balch, W., Fields, D. (2016) Light-dark calcification rates of Pleurochrysis carterae analyzed at Bigelow Laboratory in 2013 (OA Copes Coccoliths project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2016-11-04 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.664013.1 [access date]
Light-dark calcification rates of Pleurochrysis carterae. Dataset Description: <p>Laboratory experiment located at Bigelow Laboratory, East Boothbay, ME using <em>Pleurochrysis carterae</em> (NCMA strain 645), which was isolated from 41.525 deg N&nbsp;&nbsp;70.6736 deg W&nbsp;(Woods Hole, Massachusetts USA), but has been maintained in culture since 1958.</p> Methods and Sampling: <p><strong>Cultures:</strong>&nbsp;<em>Pleurochrysis </em><em>carterae</em>&nbsp;cultures were maintained in exponential growth phase under axenic conditions in semi-continuous batch culture using L1-Si media prepared on 0.2 um-filtered, UV-sterilized, autoclaved seawater.&nbsp; Cultures were acclimated to one of three&nbsp;pCO2&nbsp;treatments for &gt; 9 generations before experiments were performed.&nbsp; Cultures were maintained in an incubator at 16.5 +/- 0.5 deg C and 470 umol photons/m-2/s&nbsp;PAR.</p>
<p><strong>pCO2:</strong> Carbonate chemistry was manipulated by bubbling cultures and prepared media with 500 mL/min&nbsp;with 0.2 um-filtered 280, 380, or 750 ppm&nbsp;pCO2&nbsp;air.&nbsp; The&nbsp;pCO2&nbsp;levels of the treatment air were established using two mass flow controllers (Aalborg, Orangeburg, NY, USA) for each treatment to precisely mix in-house compressed air and pure CO2&nbsp;(Maine Oxy, Auburn, ME, USA).&nbsp; The in-house compressed air was stripped of CO2&nbsp;to less than 10 ppm CO2&nbsp;using a Puregas VCD CO2&nbsp;Adsorber (Puregas, LLC, Broomfield, CO, USA).&nbsp; The&nbsp;pCO2&nbsp;of the gas mixtures was stable to +/- 8 ppm.&nbsp;&nbsp;pCO2&nbsp;values of the cultures may be different than the target levels due to biological activity.</p>
<p><strong>Dissolution of existing coccoliths:&nbsp; </strong>Coccoliths were dissolved by 1.75 M HCl to drop the pH to 5.5 for 2 min.&nbsp; Following the dissolution (de-lithing), 1.75 M NaOH was added to bring the pH back to the respective starting pH.&nbsp; Dissolution of coccoliths was immediately confirmed by looking at the cells under cross-polarized light microscopy to verify the absence of birefringence indicative of CaCO3.&nbsp; Dissolution was further confirmed by filtering the acidified/neutralized sample onto a 0.4 um polycarbonate filter.&nbsp; Filters were mounted on stubs, sputter-coated with gold using a Denton Desk IV sputter coater (Denton Vacuum, Moorestown, NJ, USA), and imaged on a Zeiss Supra25 field emission scanning electron microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA).&nbsp; At least 15 cells per sample were imaged and the number of coccoliths/cell&nbsp;was manually counted to determine the number of coccoliths that remained after the acidification/neutralization dissolution step.</p>
<p><strong>24 h incubation in either light or dark conditions:&nbsp; </strong>To determine the number of coccoliths formed (as a proxy for calcification rate) in 24 h in either light or dark conditions, for each&nbsp;pCO2&nbsp;level, 15 mL of de-lithed culture were added to 8 scintillation vials.&nbsp; Three vials were ‘light’ replicates, three vials were ‘dark’ replicates, and two vials were poisoned with buffered formalin to serve as a ‘light’ blank and a ‘dark’ blank.&nbsp; Dark replicate and blank vials were covered in black aluminum foil and all vials were incubated together for 24 h in an incubator set at 16.5 +/- 0.5 deg C and 470 umol photons/m-2/s&nbsp;PAR on a 14-10 h light-dark cycle.&nbsp;The experiment was timed to start when the lights in the incubator turned on in the morning, thus the ‘light’ replicates were exposed to light for 14 of 24 h.&nbsp;</p>
<p><strong>Determination of attached coccoliths:&nbsp;</strong>&nbsp;Coccolith formation was assessed by counting the number of coccoliths formed during the incubation period.&nbsp; After the 24 h incubation period, each replicate and blank vial was filtered onto a 0.4 um polycarbonate filters.&nbsp; Filters were mounted on stubs, sputter-coated with gold using a Denton Desk IV sputter coater (Denton Vacuum, Moorestown, NJ, USA), and imaged on a Zeiss Supra25 field emission SEM (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA).&nbsp; At least 15 cells per replicate were imaged and the number of coccoliths/cell&nbsp;was manually counted.&nbsp; The counted coccoliths represented calcification during the 24 h incubation period and the average number of coccoliths per cell for each replicate and blank is reported.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1220068 Award URL: http://nsf.gov/awardsearch/showAward?AWD_ID=1220068
completed
William M. Balch
Bigelow Laboratory for Ocean Sciences
207-315-2567
60 Bigelow Drive
East Boothbay
ME
04544
USA
bbalch@bigelow.org
pointOfContact
David Fields
Bigelow Laboratory for Ocean Sciences
207-315-2567
60 Bigelow Dr.
East Boothbay
ME
04544
USA
dfields@bigelow.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
pCO2_treatment
sample
light_conditions
attached
attached_blankCorrected
mean_attached_blankCorrected
stdev_attached_blankCorrected
Incubator
Orion ROSS electrode
Zeiss Supra25 field emission SEM
Aalborg Mass Flow Controller
Puregas VCD CO2 Adsorber
Denton Desk IV sputter coater
theme
None, User defined
treatment
sample identification
PAR
cell_concentration
mean
standard deviation
featureType
BCO-DMO Standard Parameters
In-situ incubator
pH Sensor
Electron Microscope
Mass Flow Controller
CO2 Adsorber
Sputter Coater
instrument
BCO-DMO Standard Instruments
Balch_2013
service
Deployment Activity
Bigelow Laboratory
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=503477
Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES): Ocean Acidification (formerly CRI-OA)
NSF Climate Research Investment (CRI) activities that were initiated in 2010 are now included under Science, Engineering and Education for Sustainability NSF-Wide Investment (SEES). SEES is a portfolio of activities that highlights NSF's unique role in helping society address the challenge(s) of achieving sustainability. Detailed information about the SEES program is available from NSF (https://www.nsf.gov/funding/pgm_summ.jsp?pims_id=504707).
In recognition of the need for basic research concerning the nature, extent and impact of ocean acidification on oceanic environments in the past, present and future, the goal of the SEES: OA program is to understand (a) the chemistry and physical chemistry of ocean acidification; (b) how ocean acidification interacts with processes at the organismal level; and (c) how the earth system history informs our understanding of the effects of ocean acidification on the present day and future ocean.
Solicitations issued under this program:NSF 10-530, FY 2010-FY2011NSF 12-500, FY 2012NSF 12-600, FY 2013NSF 13-586, FY 2014
NSF 13-586 was the final solicitation that will be released for this program.
PI Meetings:1st U.S. Ocean Acidification PI Meeting(March 22-24, 2011, Woods Hole, MA)2nd U.S. Ocean Acidification PI Meeting(Sept. 18-20, 2013, Washington, DC)
3rd U.S. Ocean Acidification PI Meeting (June 9-11, 2015, Woods Hole, MA – Tentative)
NSF media releases for the Ocean Acidification Program:
Press Release 10-186 NSF Awards Grants to Study Effects of Ocean Acidification
Discovery Blue Mussels "Hang On" Along Rocky Shores: For How Long?
Discovery nsf.gov - National Science Foundation (NSF) Discoveries - Trouble in Paradise: Ocean Acidification This Way Comes - US National Science Foundation (NSF)
Press Release 12-179 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: Finding New Answers Through National Science Foundation Research Grants - US National Science Foundation (NSF)
Press Release 13-102 World Oceans Month Brings Mixed News for Oysters
Press Release 13-108 nsf.gov - National Science Foundation (NSF) News - Natural Underwater Springs Show How Coral Reefs Respond to Ocean Acidification - US National Science Foundation (NSF)
Press Release 13-148 Ocean acidification: Making new discoveries through National Science Foundation research grants
Press Release 13-148 - Video nsf.gov - News - Video - NSF Ocean Sciences Division Director David Conover answers questions about ocean acidification. - US National Science Foundation (NSF)
Press Release 14-010 nsf.gov - National Science Foundation (NSF) News - Palau's coral reefs surprisingly resistant to ocean acidification - US National Science Foundation (NSF)
Press Release 14-116 nsf.gov - National Science Foundation (NSF) News - Ocean Acidification: NSF awards $11.4 million in new grants to study effects on marine ecosystems - US National Science Foundation (NSF)
SEES-OA
largerWorkCitation
program
Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps
https://www.bco-dmo.org/project/514415
Effects of ocean acidification on Emiliania huxleyi and Calanus finmarchicus; insights into the oceanic alkalinity and biological carbon pumps
<p><em>(Extracted from the NSF award abstract)</em></p>
<p>Ocean acidification is one of the most pressing marine science issues of our time, with potential biological impacts spanning all marine phyla and potential societal impacts affecting man's relationship to the sea. Rising levels of atmospheric pCO2 are increasing the acidity of the world oceans. It is generally held that average surface ocean pH has already declined by 0.1 pH units relative to the pre-industrial level (Orr et al., 2005), and is projected to decrease 0.3 to 0.46 units by the end of this century, depending on CO2 emission scenarios (Caldeira and Wickett, 2005). The overall goal of this research is to parameterize how changes in pCO2 levels could alter the biological and alkalinity pumps of the world ocean. Specifically, the direct and indirect effects of ocean acidification will be examined within a simple, controlled predator/prey system containing a single prey phytoplankton species (the coccolithophore, Emiliania huxleyi) and a single predator (the oceanic metazoan grazer, Calanus finmarchicus). The experiments are designed to elucidate both direct effects (i.e. effects of ocean acidification on the individual organisms only) and interactive effects (i.e. effects on the combined predator/prey system). Interactive experiments with phytoplankton prey and zooplankton predator are a critical starting point for predicting the overall impact of ocean acidification in marine ecosystems. To meet these goals, a state-of-the-art facility will be constructed with growth chambers that are calibrated and have highly-controlled pH and alkalinity levels. The strength of this approach lies in meticulous calibration and redundant measurements that will be made to ensure that conditions within the chambers are well described and tightly monitored for DIC levels. Growth and calcification rates in coccolithophores and the developmental rates, morphological and behavioral effects on copepods will be measured. The PIC and POC in the algae and the excreted fecal pellets will be monitored for changes in the PIC/POC ratio, a key parameter for modeling feedback mechanisms for rising pCO2 levels. In addition, 14C experiments are planned to measure calcification rates in coccolithophores and dissolution rates as a result of grazing. These key experiments will verify closure in the mass balance of PIC, allowing the determination of actual dissolution rates of PIC within the guts of copepod grazers.</p>
OA_Copes_Coccoliths
largerWorkCitation
project
eng; USA
oceans
Bigelow Laboratory
2013-01-01
2013-12-31
Laboratory experiments; East Boothbay, Maine
0
BCO-DMO catalogue of parameters from Light-dark calcification rates of Pleurochrysis carterae analyzed at Bigelow Laboratory in 2013 (OA Copes Coccoliths project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/664032.rdf
Name: pCO2_treatment
Units: parts per million (ppm)
Description: The independent variable - one of three pCO2 levels (280 ppm, 380 ppm, or 750 ppm) These treatment levels are nominal values as they represent the target pCO2 for each treatment.
http://lod.bco-dmo.org/id/dataset-parameter/664033.rdf
Name: sample
Units: unitless
Description: For each pCO2 treatment there are three types of samples;
After de-lithing: These samples were taken immediately after the coccolithophores were acidified to dissolve their coccoliths and then neutralized to return the culture pH to the original pH. These samples were taken before the 24 h incubation period and therefore do not have a Light or Dark treatment.
Replicate: These represent the three replicates for each light condition and each CO2 treatment. These samples were taken after the cells incubated in either light or dark conditions for 24 h.
Blank: These samples were killed by the addition of formalin but were allowed to incubate in either light or dark conditions for 24 h.
http://lod.bco-dmo.org/id/dataset-parameter/664034.rdf
Name: light_conditions
Units: unitless
Description: This identifies whether the sample was incubated for 24 h in light (470 umol photons/m-2/s PAR) or dark (0 umol photons/m-2/s PAR) conditions.
http://lod.bco-dmo.org/id/dataset-parameter/664035.rdf
Name: attached
Units: coccoliths per cell
Description: This is the number of attached coccoliths observed per cell. This number represents an average of counts from at least 15 cells per replicate or sample.
http://lod.bco-dmo.org/id/dataset-parameter/664036.rdf
Name: attached_blankCorrected
Units: coccoliths per cell
Description: This is the number of attached coccoliths observed per cell with the number of coccoliths observed per cell from the blank samples subtracted to account for any coccoliths that might have remained after cells were de-lithed.
http://lod.bco-dmo.org/id/dataset-parameter/664037.rdf
Name: mean_attached_blankCorrected
Units: coccoliths per cell
Description: The average value for each set of three replicates.
http://lod.bco-dmo.org/id/dataset-parameter/664038.rdf
Name: stdev_attached_blankCorrected
Units: coccoliths per cell
Description: The standard deviation for each set of three replicates.
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1142
https://darchive.mblwhoilibrary.org/bitstream/1912/24045/1/dataset-664013_pleurochrysis-carterae-light-dark-calcification__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.664013.1
download
onLine
dataset
<p><strong>Cultures:</strong>&nbsp;<em>Pleurochrysis </em><em>carterae</em>&nbsp;cultures were maintained in exponential growth phase under axenic conditions in semi-continuous batch culture using L1-Si media prepared on 0.2 um-filtered, UV-sterilized, autoclaved seawater.&nbsp; Cultures were acclimated to one of three&nbsp;pCO2&nbsp;treatments for &gt; 9 generations before experiments were performed.&nbsp; Cultures were maintained in an incubator at 16.5 +/- 0.5 deg C and 470 umol photons/m-2/s&nbsp;PAR.</p>
<p><strong>pCO2:</strong> Carbonate chemistry was manipulated by bubbling cultures and prepared media with 500 mL/min&nbsp;with 0.2 um-filtered 280, 380, or 750 ppm&nbsp;pCO2&nbsp;air.&nbsp; The&nbsp;pCO2&nbsp;levels of the treatment air were established using two mass flow controllers (Aalborg, Orangeburg, NY, USA) for each treatment to precisely mix in-house compressed air and pure CO2&nbsp;(Maine Oxy, Auburn, ME, USA).&nbsp; The in-house compressed air was stripped of CO2&nbsp;to less than 10 ppm CO2&nbsp;using a Puregas VCD CO2&nbsp;Adsorber (Puregas, LLC, Broomfield, CO, USA).&nbsp; The&nbsp;pCO2&nbsp;of the gas mixtures was stable to +/- 8 ppm.&nbsp;&nbsp;pCO2&nbsp;values of the cultures may be different than the target levels due to biological activity.</p>
<p><strong>Dissolution of existing coccoliths:&nbsp; </strong>Coccoliths were dissolved by 1.75 M HCl to drop the pH to 5.5 for 2 min.&nbsp; Following the dissolution (de-lithing), 1.75 M NaOH was added to bring the pH back to the respective starting pH.&nbsp; Dissolution of coccoliths was immediately confirmed by looking at the cells under cross-polarized light microscopy to verify the absence of birefringence indicative of CaCO3.&nbsp; Dissolution was further confirmed by filtering the acidified/neutralized sample onto a 0.4 um polycarbonate filter.&nbsp; Filters were mounted on stubs, sputter-coated with gold using a Denton Desk IV sputter coater (Denton Vacuum, Moorestown, NJ, USA), and imaged on a Zeiss Supra25 field emission scanning electron microscope (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA).&nbsp; At least 15 cells per sample were imaged and the number of coccoliths/cell&nbsp;was manually counted to determine the number of coccoliths that remained after the acidification/neutralization dissolution step.</p>
<p><strong>24 h incubation in either light or dark conditions:&nbsp; </strong>To determine the number of coccoliths formed (as a proxy for calcification rate) in 24 h in either light or dark conditions, for each&nbsp;pCO2&nbsp;level, 15 mL of de-lithed culture were added to 8 scintillation vials.&nbsp; Three vials were ‘light’ replicates, three vials were ‘dark’ replicates, and two vials were poisoned with buffered formalin to serve as a ‘light’ blank and a ‘dark’ blank.&nbsp; Dark replicate and blank vials were covered in black aluminum foil and all vials were incubated together for 24 h in an incubator set at 16.5 +/- 0.5 deg C and 470 umol photons/m-2/s&nbsp;PAR on a 14-10 h light-dark cycle.&nbsp;The experiment was timed to start when the lights in the incubator turned on in the morning, thus the ‘light’ replicates were exposed to light for 14 of 24 h.&nbsp;</p>
<p><strong>Determination of attached coccoliths:&nbsp;</strong>&nbsp;Coccolith formation was assessed by counting the number of coccoliths formed during the incubation period.&nbsp; After the 24 h incubation period, each replicate and blank vial was filtered onto a 0.4 um polycarbonate filters.&nbsp; Filters were mounted on stubs, sputter-coated with gold using a Denton Desk IV sputter coater (Denton Vacuum, Moorestown, NJ, USA), and imaged on a Zeiss Supra25 field emission SEM (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA).&nbsp; At least 15 cells per replicate were imaged and the number of coccoliths/cell&nbsp;was manually counted.&nbsp; The counted coccoliths represented calcification during the 24 h incubation period and the average number of coccoliths per cell for each replicate and blank is reported.</p>
Specified by the Principal Investigator(s)
<p><strong>Blank correction: </strong>For each replicate, the number of coccoliths per cell determined from the corresponding blank sample (same&nbsp;pCO2&nbsp;and light conditions) was subtracted from the number of coccoliths per cell determined from the replicate.&nbsp; If the&nbsp;blank-correction&nbsp;resulted in a negative value, the value was set to 0 coccoliths per cell.</p>
<p><strong>Verification of de-lithing:&nbsp; </strong>The number of coccoliths per cell immediately after the de-lithing was not explicitly used in any calculations.&nbsp; However, because so few liths were formed in the ‘dark’ replicates, we performed a 2-way ANOVA to compare the number of coccoliths per cell immediately after the de-lithing with the number of coccoliths per cell in the dark incubation treatments.&nbsp; This test showed that there were significantly more coccoliths per cell for the dark incubation replicates, relative to the samples collected immediately after the de-lithing, which supports the claim that there was calcification in the dark.&nbsp; The results of this statistical test are presented in our paper, so those values are not included in the dataset.&nbsp;&nbsp;</p>
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added underscores and removed spaces and units from column names<br />
- changed column names to comply with BCO-DMO standards.<br />
- replaced all "n/a" with "nd," and filled in blank cells with "nd"</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Incubator
Incubator
PI Supplied Instrument Name: Incubator PI Supplied Instrument Description:Dark replicate and blank vials were covered in black aluminum foil and all vials were incubated together for 24 h in an incubator set at 16.5 +/- 0.5 deg C and 470 umol photons/m-2/s PAR on a 14-10 h light-dark cycle. Instrument Name: In-situ incubator Instrument Short Name:in-situ incubator Instrument Description: A device on a ship or in the laboratory that holds water samples under controlled conditions of temperature and possibly illumination. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/82/
Orion ROSS electrode
Orion ROSS electrode
PI Supplied Instrument Name: Orion ROSS electrode PI Supplied Instrument Description:Orion ROSS electrode was connected to an Orion Star A211 Benchtop pH meter (ThermoFisher Scientific, Waltham, MA, USA) Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions.
The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).
Zeiss Supra25 field emission SEM
Zeiss Supra25 field emission SEM
PI Supplied Instrument Name: Zeiss Supra25 field emission SEM PI Supplied Instrument Description:Carl Zeiss Microscopy, LLC, Thornwood, NY, USA Instrument Name: Electron Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of electrons behaving as waves. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB07/
Aalborg Mass Flow Controller
Aalborg Mass Flow Controller
PI Supplied Instrument Name: Aalborg Mass Flow Controller PI Supplied Instrument Description:Indicate and control set flow rates of gases. Manufactured in Orangeburg, NY USA. Instrument Name: Mass Flow Controller Instrument Short Name:MFC Instrument Description: Mass Flow Controller (MFC) - A device used to measure and control the flow of fluids and gases
Puregas VCD CO2 Adsorber
Puregas VCD CO2 Adsorber
PI Supplied Instrument Name: Puregas VCD CO2 Adsorber PI Supplied Instrument Description:Instrument stripped compressed air of CO2 Instrument Name: CO2 Adsorber Instrument Short Name:CO2 Adsorber Instrument Description: CO2 Adsorber - an instrument designed to remove CO2 and moisture from compressed air.
Denton Desk IV sputter coater
Denton Desk IV sputter coater
PI Supplied Instrument Name: Denton Desk IV sputter coater PI Supplied Instrument Description:Filters were mounted on stubs, sputter-coated with gold using a Denton Desk IV sputter coater (Denton Vacuum, Moorestown, NJ, USA). Instrument Name: Sputter Coater Instrument Short Name:Sputter Coater Instrument Description: Sputter coating is the standard method for preparing non-conducting or poorly conducting specimens prior to observation in a scanning electron microscope (SEM)
Deployment: Balch_2013
Balch_2013
lab Bigelow
laboratory
Balch_2013
William M. Balch
Bigelow Laboratory for Ocean Sciences
lab Bigelow
laboratory