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Dataset Title:  Presence and absence of iron and light-related functional genes collected on
the Gould (LMG1411) cruise in the Western Antarctica Peninsula during
2014 (Polar Transcriptomes project)
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Institution:  BCO-DMO   (Dataset ID: bcodmo_dataset_665407)
Information:  Summary ? | License ? | ISO 19115 | Metadata | Background (external link) | Files | Make a graph
 
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Constraint #2 ?
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 species (unitless) ?          "Actinocyclus_actin..."    "Thalassiosira_anta..."
 description (unitless) ?          "High-affinity_Fe_u..."    "Urea_cycle"
 protein (unitless) ?          "AOX"    "Rhodopsin"
 RPKM (unitless) ?          2    45293
 evalue (unitless) ?          -3.0E-6    0.0
 KO_num (unitless) ?          "K00366"    "NaN"
 accession_link (unitless) ?          "    "NaN"
 
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The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  species {
    String bcodmo_name "species";
    String description "Species analyzed";
    String long_name "Species";
    String units "unitless";
  }
  description {
    String bcodmo_name "sample_descrip";
    String description "Category of genes of interest";
    String long_name "Description";
    String units "unitless";
  }
  protein {
    String bcodmo_name "sample";
    String description "Gene name";
    String long_name "Protein";
    String nerc_identifier "https://vocab.nerc.ac.uk/collection/P02/current/ACYC/";
    String units "unitless";
  }
  RPKM {
    Int32 _FillValue 2147483647;
    Int32 actual_range 2, 45293;
    String bcodmo_name "unknown";
    String description "Presence of a gene is denoted with semi-qualitative RPKM (Reads Per Kilobase of transcript per Million) values";
    String long_name "RPKM";
    String units "unitless";
  }
  evalue {
    Float64 _FillValue NaN;
    Float64 actual_range -3.0e-6, 0.0;
    String bcodmo_name "unknown";
    String description "E-values";
    String long_name "Evalue";
    String units "unitless";
  }
  KO_num {
    String bcodmo_name "accession number";
    String description "Listed for genes that had a homolog in the KEGG database";
    String long_name "KO Num";
    String units "unitless";
  }
  accession_link {
    String bcodmo_name "accession number";
    String description "Accession link for K0 number";
    String long_name "Accession Link";
    String units "unitless";
  }
 }
  NC_GLOBAL {
    String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv";
    String acquisition_description 
"Nine species of diatoms were isolated from the Western Antarctic Peninsula
along the PalmerLTER sampling grid in 2013 and 2014. Isolations were performed
using an Olympus CKX41 inverted microscope by single cell isolation with a
micropipette (Anderson 2005). Diatom species were identified by morphological
characterization and 18S rRNA gene (rDNA) sequencing. DNA was extracted with
the DNeasy Plant Mini Kit according to the manufacturer\\u2019s protocols
(Qiagen). Amplification of the nuclear 18S rDNA region was achieved with
standard PCR protocols using eukaryotic-specific, universal 18S forward and
reverse primers. Primer sequences were obtained from Medlin et al. (1982). The
length of the region amplified is approximately 1800 base pairs (bp
).\\u00a0Pseudo-nitzschia\\u00a0species are often difficult to identify by their
18S rDNA sequence, therefore, additional support of the taxonomic
identification of\\u00a0P.\\u00a0subcurvata\\u00a0was provided through sequencing
of the 18S-ITS1-5.8S regions. Amplification of this region was performed with
the 18SF-euk and 5.8SR_euk primers of Hubbard et al. (2008). PCR products were
purified using either QIAquick PCR Purification Kit (Qiagen) or ExoSAP-IT
(Affymetrix) and sequenced by Sanger DNA sequencing (Genewiz). Sequences were
edited using Geneious Pro software
([http://www.geneious.com](\\\\\"http://www.geneious.com\\\\\"), Kearse et al.,
2012) and BLASTn sequence homology searches were performed against the NCBI
nucleotide non-redundant (nr) database to determine species with a cutoff
identity of 98%.
 
Diatom phylogenetic analysis was performed with Geneious Pro and included 71
additional diatom 18S rDNA sequences from publically available genomes and
transcriptomes, including those in the MMETSP database. Diatom sequences were
trimmed to the same length and aligned with MUSCLE (Edgar 2004). A
phylogenetic tree was created in Mega with the Maximum-likelihood method of
tree reconstruction, the Jukes-Cantor genetic distance model (Jukes and Cantor
1969), and 100 bootstrap replicates.
 
Isolates were maintained at 4 deg C in constant irradiance at intensities of
either 10\\u00a0umol\\u00a0photons m-2\\u00a0s-1\\u00a0(low light) or
90\\u00a0umol\\u00a0photons m-2\\u00a0s-1\\u00a0(growth saturating light) and with
media containing high and low iron concentrations. Cultures were grown in the
synthetic seawater medium, AQUIL, enriched with filter sterilized vitamin and
trace metal ion buffer containing 100\\u00a0umol\\u00a0L-1\\u00a0EDTA. The growth
media also contained 300 \\u03bcmol L-1\\u00a0nitrate,
200\\u00a0umol\\u00a0L-1\\u00a0silicic acid and
20\\u00a0umol\\u00a0L-1\\u00a0phosphate. Premixed Fe-EDTA (1:1) was added
separately for total iron concentrations of either 1370 nmol L-1\\u00a0or 3.1
nmol L-1. Cultures were grown in acid-washed 28 mL polycarbonate centrifuge
tubes (Nalgene) and maintained in exponential phase by dilution. Specific
growth rates of successive transfers were calculated from the linear
regression of the natural\\u00a0log of\\u00a0in
vivo\\u00a0chlorophyll\\u00a0a\\u00a0fluorescence using a Turner 10-AU
fluorometer (Brand et al. 1981).\\u00a0
 
Photophysiological parameters were measured with a Fluorescence Induction
Relaxation System (FIRe) (Satatlantic). Samples were dark acclimated for at
least 10 minutes and measurements were taken of each culture for
photosynthetic efficiency (Fv:Fm), and functional absorption cross-section of
PSII (oPSII\\u00a0[A2\\u00a0quanta-1]). FIRe parameters were set to measure
single turnover flash of PSII reaction centers (single closure event) with a
sample delay of 100, and a total of 50 samples (Gorbunov and Falkowski 2004).
 
\\u00a0";
    String awards_0_award_nid "653228";
    String awards_0_award_number "PLR-1341479";
    String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1341479";
    String awards_0_funder_name "NSF Division of Ocean Sciences";
    String awards_0_funding_acronym "NSF OCE";
    String awards_0_funding_source_nid "355";
    String awards_0_program_manager "Dr Chris H. Fritsen";
    String awards_0_program_manager_nid "50502";
    String cdm_data_type "Other";
    String comment 
"Presence and Absence Data 
  Adrian Marchetti, PI 
  Version 11 October 2016";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_type "institution";
    String creator_url "https://www.bco-dmo.org/";
    String data_source "extract_data_as_tsv version 2.3  19 Dec 2019";
    String date_created "2016-11-21T20:13:31Z";
    String date_modified "2019-04-17T20:51:30Z";
    String defaultDataQuery "&time<now";
    String doi "10.1575/1912/bco-dmo.665407.1";
    String history 
"2022-08-17T10:55:48Z (local files)
2022-08-17T10:55:48Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_665407.html";
    String infoUrl "https://www.bco-dmo.org/dataset/665407";
    String institution "BCO-DMO";
    String instruments_0_acronym "Inverted Microscope";
    String instruments_0_dataset_instrument_description "Used to perform isolations";
    String instruments_0_dataset_instrument_nid "665414";
    String instruments_0_description 
"An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).

Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g. a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. Inverted microscopes are also used in micromanipulation applications where space above the specimen is required for manipulator mechanisms and the microtools they hold, and in metallurgical applications where polished samples can be placed on top of the stage and viewed from underneath using reflecting objectives.

The stage on an inverted microscope is usually fixed, and focus is adjusted by moving the objective lens along a vertical axis to bring it closer to or further from the specimen. The focus mechanism typically has a dual concentric knob for coarse and fine adjustment. Depending on the size of the microscope, four to six objective lenses of different magnifications may be fitted to a rotating turret known as a nosepiece. These microscopes may also be fitted with accessories for fitting still and video cameras, fluorescence illumination, confocal scanning and many other applications.";
    String instruments_0_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB05/";
    String instruments_0_instrument_name "Inverted Microscope";
    String instruments_0_instrument_nid "675";
    String instruments_0_supplied_name "Olympus CKX41";
    String instruments_1_acronym "Bioanalyzer";
    String instruments_1_dataset_instrument_description "Used to determine RNA integrity";
    String instruments_1_dataset_instrument_nid "665417";
    String instruments_1_description "A Bioanalyzer is a laboratory instrument that provides the sizing and quantification of DNA, RNA, and proteins. One example is the Agilent Bioanalyzer 2100.";
    String instruments_1_instrument_name "Bioanalyzer";
    String instruments_1_instrument_nid "626182";
    String instruments_1_supplied_name "Agilent Bioanalyzer 2100";
    String keywords "accession, accession_link, bco, bco-dmo, biological, chemical, data, dataset, description, dmo, erddap, evalue, KO_num, link, management, num, oceanography, office, preliminary, protein, rpkm, species";
    String license "https://www.bco-dmo.org/dataset/665407/license";
    String metadata_source "https://www.bco-dmo.org/api/dataset/665407";
    String param_mapping "{'665407': {}}";
    String parameter_source "https://www.bco-dmo.org/mapserver/dataset/665407/parameters";
    String people_0_affiliation "University of North Carolina at Chapel Hill";
    String people_0_affiliation_acronym "UNC-Chapel Hill";
    String people_0_person_name "Adrian Marchetti";
    String people_0_person_nid "527120";
    String people_0_role "Principal Investigator";
    String people_0_role_type "originator";
    String people_1_affiliation "University of North Carolina at Chapel Hill";
    String people_1_affiliation_acronym "UNC-Chapel Hill";
    String people_1_person_name "Adrian Marchetti";
    String people_1_person_nid "527120";
    String people_1_role "Contact";
    String people_1_role_type "related";
    String people_2_affiliation "Woods Hole Oceanographic Institution";
    String people_2_affiliation_acronym "WHOI BCO-DMO";
    String people_2_person_name "Hannah Ake";
    String people_2_person_nid "650173";
    String people_2_role "BCO-DMO Data Manager";
    String people_2_role_type "related";
    String project "Polar_Transcriptomes";
    String projects_0_acronym "Polar_Transcriptomes";
    String projects_0_description 
"The Southern Ocean surrounding Antarctica is changing rapidly in response to Earth's warming climate. These changes will undoubtedly influence communities of primary producers (the organisms at the base of the food chain, particularly plant-like organisms using sunlight for energy) by altering conditions that influence their growth and composition. Because primary producers such as phytoplankton play an important role in global biogeochemical cycling, it is essential to understand how they will respond to changes in their environment. The growth of phytoplankton in certain regions of the Southern Ocean is constrained by steep gradients in chemical and physical properties that vary in both space and time. Light and iron have been identified as key variables influencing phytoplankton abundance and distribution within Antarctic waters. Microscopic algae known as diatoms are dominant members of the phytoplankton and sea ice communities, accounting for significant proportions of primary production. The overall objective of this project is to identify the molecular bases for the physiological responses of polar diatoms to varying light and iron conditions. The project should provide a means of evaluating the extent these factors regulate diatom growth and influence net community productivity in Antarctic waters. The project will also further the NSF goals of making scientific discoveries available to the general public and of training new generations of scientists. It will facilitate the teaching and learning of polar-related topics by translating the research objectives into readily accessible educational materials for middle-school students. This project will also provide funding to enable a graduate student and several undergraduate students to be trained in the techniques and perspectives of modern biology.
Although numerous studies have investigated how polar diatoms are affected by varying light and iron, the cellular mechanisms leading to their distinct physiological responses remain unknown. Using comparative transcriptomics, the expression patterns of key genes and metabolic pathways in several ecologically important polar diatoms recently isolated from Antarctic waters and grown under varying iron and irradiance conditions will be examined. In addition, molecular indicators for iron and light limitation will be developed within these polar diatoms through the identification of iron- and light-responsive genes -- the expression patterns of which can be used to determine their physiological status. Upon verification in laboratory cultures, these indicators will be utilized by way of metatranscriptomic sequencing to examine iron and light limitation in natural diatom assemblages collected along environmental gradients in Western Antarctic Peninsula waters. In order to fully understand the role phytoplankton play in Southern Ocean biogeochemical cycles, dependable methods that provide a means of elucidating the physiological status of phytoplankton at any given time and location are essential.";
    String projects_0_end_date "2017-07";
    String projects_0_geolocation "Antarctica";
    String projects_0_name "Iron and Light Limitation in Ecologically Important Polar Diatoms: Comparative Transcriptomics and Development of Molecular Indicators";
    String projects_0_project_nid "653229";
    String projects_0_project_website "http://www.nsf.gov/awardsearch/showAward?AWD_ID=1341479";
    String projects_0_start_date "2014-08";
    String publisher_name "Biological and Chemical Oceanographic Data Management Office (BCO-DMO)";
    String publisher_type "institution";
    String sourceUrl "(local files)";
    String standard_name_vocabulary "CF Standard Name Table v55";
    String summary "Presence and absence of iron and light-related functional genes collected on the Gould (LMG1411) cruise in the Western Antarctica Peninsula during 2014 (Polar Transcriptomes project)";
    String title "Presence and absence of iron and light-related functional genes collected on the Gould (LMG1411) cruise in the Western Antarctica Peninsula during 2014 (Polar Transcriptomes project)";
    String version "1";
    String xml_source "osprey2erddap.update_xml() v1.3";
  }
}

 

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