http://lod.bco-dmo.org/id/dataset/712377
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2017-08-08
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Acropora cervicornis growth rates under different pH and temperature treatments from experiments at Summerland Key, Florida in September of 2016
2017-10-05
publication
2017-10-05
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-10-18
publication
https://doi.org/10.1575/1912/bco-dmo.712377.1
Erinn Muller
Mote Marine Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Muller, E. (2017) Acropora cervicornis growth rates under different pH and temperature treatments from experiments at Summerland Key, Florida in September of 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2017-10-05 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.712377.1 [access date]
calcification rates of Acropora cervicornis Dataset Description: <p>This dataset contains Acropora cervicornis calcification data from experiments conducted in tanks at&nbsp;Summerland Key, Florida&nbsp;(24.6616,-81.4538) between 2016-09-02 and 2016-09-10 with corals from a&nbsp;nursery located near Looe Key Reef&nbsp;(24.5636, -81.2786).</p> Methods and Sampling: <p>Physiological Methods</p>
<p>Photosynthesis, respiration, and calcification measurements were performed on each fragment using 300 mL temperature-controlled respirometry chambers filled with seawater from the treatment aquaria that was continuously stirred with a magnetic stir bar. &nbsp;The chambers were used to assess the rates of respiration (Rd) in the dark and rates of photosynthesis (Pn) and calcification in the light. &nbsp;Light was supplied by a series of blue and red LEDs with adjustable intensity (150 uMol quanta m-2 sec-1). &nbsp;Water samples were taken from each chamber prior to a cycle and also at the end of both dark and light incubations (60 minutes each) for measurements of pHT (pH on the total scale) and total alkalinity (AT) as described in Martin and Gattuso (2009). &nbsp;</p>
<p>Calcification - Total alkalinity (TA) values were measured using an automatic potentiometric titrator (Metrohm 807 Titrando, Riverview, FL) to the second end point of a 15.3-g accurately weighed seawater sample. &nbsp;Total alkalinity values were then computed using the Gran equation (DOE, 1994) with pH values lower than 3.9 for creating the Gran plot. The pH electrodes (Metrohm 807 Titrando) were calibrated daily as described above. &nbsp;The acid titrant concentration was 0.05N HCl (JT Baker, Phillipsburg, NJ). &nbsp;Alkalinity was calculated using the first derivative of the curve for the evaluation of the exact end point. &nbsp;Standards for total seawater alkalinity and provided by Dickson were run daily (Dickson, 2007). &nbsp;The differences between duplicate samples and standards were less than 5 uEq kg-1 (for calibration of the titrator, differences were measured between triplicate samples). &nbsp;Water samples were analyzed immediately or stored in darkness at 4C and processed within 24 hours of collection. &nbsp;</p>
<p>Dickson AG, Sabine CL, and Christian JR (2007) Guide to best practices for ocean CO2 measurements: PICES Special Publication. 3, 191 p.</p>
<p>Martin S and Gattuso J-P (2009) Response of Mediterranean coralline algae to ocean acidification and elevated temperature. Glob Change Biol 15:2089-2100.&nbsp;<br />
Marubini F and Thake B (1999) Bicarbonate addition promotes coral growth. Limnol and Oceanogr 44: 716-720.</p>
<p>Riebesell U, Fabry VJ, Hansson L, and Gattuso JP (2010) Guide to best practices for ocean acidification research and data reporting. European Commission, European Research Area. Brussels. 258 p</p>
<p>&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1452538 Award URL: http://www.nsf.gov/awardsearch/showAward?AWD_ID=1452538&HistoricalAwards=false
completed
Erinn Muller
Mote Marine Laboratory
941-388-4441 x 310
1600 Ken Thompson Parkway
Sarasota
FL
34241
USA
emuller@mote.org
pointOfContact
asNeeded
Dataset Version: 1
Unknown
pH
Temp
Tank
Chamber
Genotype
Cycle
Calcification
Date
YSI Pro 2030
Mettler Toledo SevenGo Pro
Metrohm 807 Titrando
theme
None, User defined
pH
water temperature
tank
treatment
sample description
calcification
date
featureType
BCO-DMO Standard Parameters
YSI Professional Plus Multi-Parameter Probe
pH Sensor
Automatic titrator
instrument
BCO-DMO Standard Instruments
Muller_Looe_Key_Reef_Acropora
service
Deployment Activity
Looe Key Reef
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys
https://www.bco-dmo.org/project/642850
CAREER: Applying phenotypic variability to identify resilient Acropora cervicornis genotypes in the Florida Keys
<p>Caribbean staghorn coral was one of the most common corals within reefs of the Florida Keys several decades ago. Over the last 40 years disease, bleaching, overfishing and habitat degradation caused a 95% reduction of the population. Staghorn coral is now listed as threatened under the U.S. Endangered Species Act of 1973. Within the past few years, millions of dollars have been invested for the purpose of restoring the population of staghorn coral within Florida and the U.S. Virgin Islands. Significant effort has been placed on maintaining and propagating corals of known genotypes within coral nurseries for the purpose of outplanting. However, little is known about the individual genotypes that are currently being outplanted from nurseries onto coral reefs. Are the genotypes being used for outplanting resilient enough to survive the three major stressors affecting the population in the Florida Keys: disease, high water temperatures, and ocean acidification? The research within the present study will be the first step in answering this critically important question. The funded project will additionally develop a research-based afterschool program with K-12 students in the Florida Keys and U.S. Virgin Islands that emphasizes an inquiry-based curriculum, STEM research activities, and peer-to-peer mentoring. The information from the present study will help scientists predict the likelihood of species persistence within the lower Florida Keys under future climate-change and ocean-acidification scenarios. Results of this research will also help guide restoration efforts throughout Florida and the Caribbean, and lead to more informative, science-based restoration activities.</p>
<p>Acropora cervicornis dominated shallow-water reefs within the Florida Keys for at least the last half a million years, but the population has recently declined due to multiple stressors. Understanding the current population level of resilience to three major threats - disease outbreaks, high water temperatures, and ocean acidification conditions - is critical for the preservation of this threatened species. Results from the present study will answer the primary research question: will representative genotypes from the lower Florida Keys provide enough phenotypic variation for this threatened species to survive in the future? The present proposal will couple controlled laboratory challenge experiments with field data and modeling applications, and collaborate with local educators to fulfill five objectives: 1) identify A. cervicornis genotypes resistant to disease, 2) identify A. cervicornis genotypes resilient to high water temperature and ocean acidification conditions, 3) quantify how high water temperature and ocean acidification conditions impact disease dynamics on A. cervicornis; 4) determine tradeoffs in life-history traits because of resilience factors; and 5) apply a trait-based model, which will predict genotypic structure of a population under different environmental scenarios.</p>
Resilient Acerv
largerWorkCitation
project
eng; USA
biota
Looe Key Reef
-81.453789
-81.2786
24.5636
24.661603
2016-09-02
2016-09-10
Florida Keys, Summerland Key, FL 24.563595°, -81.278572°
0
BCO-DMO catalogue of parameters from Acropora cervicornis growth rates under different pH and temperature treatments from experiments at Summerland Key, Florida in September of 2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/712432.rdf
Name: pH
Units: unitless
Description: Treatment pH level; ambient = 8.1 pH; hCO2 = 7.7 pH
http://lod.bco-dmo.org/id/dataset-parameter/712433.rdf
Name: Temp
Units: Celsius
Description: Treatment temperature level
http://lod.bco-dmo.org/id/dataset-parameter/712434.rdf
Name: Tank
Units: unitless
Description: Tank number that held the particular coral fragment
http://lod.bco-dmo.org/id/dataset-parameter/712435.rdf
Name: Chamber
Units: unitless
Description: Chamber number that held the coral fragment during the light and dark cycle
http://lod.bco-dmo.org/id/dataset-parameter/712436.rdf
Name: Genotype
Units: unitless
Description: Genotype number of the coral animal for each fragment
http://lod.bco-dmo.org/id/dataset-parameter/712437.rdf
Name: Cycle
Units: unitless
Description: Characterizes whether the coral was exposed to light or held in the dark prior to final measurements
http://lod.bco-dmo.org/id/dataset-parameter/712438.rdf
Name: Calcification
Units: micromoles of calcium carbonate per centimeter squred per hour (CaCO3/cm2/h)
Description: Rate at which the coral is utilizing calcium carbonate for skeletal growth
http://lod.bco-dmo.org/id/dataset-parameter/716209.rdf
Name: Date
Units: unitless
Description: Date in format yyyy-mm-dd
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
21620
https://darchive.mblwhoilibrary.org/bitstream/1912/24701/1/dataset-712377_acropora-cervicornis-calcification-rates__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.712377.1
download
onLine
dataset
<p>Physiological Methods</p>
<p>Photosynthesis, respiration, and calcification measurements were performed on each fragment using 300 mL temperature-controlled respirometry chambers filled with seawater from the treatment aquaria that was continuously stirred with a magnetic stir bar. &nbsp;The chambers were used to assess the rates of respiration (Rd) in the dark and rates of photosynthesis (Pn) and calcification in the light. &nbsp;Light was supplied by a series of blue and red LEDs with adjustable intensity (150 uMol quanta m-2 sec-1). &nbsp;Water samples were taken from each chamber prior to a cycle and also at the end of both dark and light incubations (60 minutes each) for measurements of pHT (pH on the total scale) and total alkalinity (AT) as described in Martin and Gattuso (2009). &nbsp;</p>
<p>Calcification - Total alkalinity (TA) values were measured using an automatic potentiometric titrator (Metrohm 807 Titrando, Riverview, FL) to the second end point of a 15.3-g accurately weighed seawater sample. &nbsp;Total alkalinity values were then computed using the Gran equation (DOE, 1994) with pH values lower than 3.9 for creating the Gran plot. The pH electrodes (Metrohm 807 Titrando) were calibrated daily as described above. &nbsp;The acid titrant concentration was 0.05N HCl (JT Baker, Phillipsburg, NJ). &nbsp;Alkalinity was calculated using the first derivative of the curve for the evaluation of the exact end point. &nbsp;Standards for total seawater alkalinity and provided by Dickson were run daily (Dickson, 2007). &nbsp;The differences between duplicate samples and standards were less than 5 uEq kg-1 (for calibration of the titrator, differences were measured between triplicate samples). &nbsp;Water samples were analyzed immediately or stored in darkness at 4C and processed within 24 hours of collection. &nbsp;</p>
<p>Dickson AG, Sabine CL, and Christian JR (2007) Guide to best practices for ocean CO2 measurements: PICES Special Publication. 3, 191 p.</p>
<p>Martin S and Gattuso J-P (2009) Response of Mediterranean coralline algae to ocean acidification and elevated temperature. Glob Change Biol 15:2089-2100.&nbsp;<br />
Marubini F and Thake B (1999) Bicarbonate addition promotes coral growth. Limnol and Oceanogr 44: 716-720.</p>
<p>Riebesell U, Fabry VJ, Hansson L, and Gattuso JP (2010) Guide to best practices for ocean acidification research and data reporting. European Commission, European Research Area. Brussels. 258 p</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Calcification values were calculated using the alkalinity anomaly method (Riebesell et al., 2010).&nbsp; Calcification rates were normalized to surface area.</p>
<p>Riebesell U, Fabry VJ, Hansson L, and Gattuso JP (2010) Guide to best practices for ocean acidification research and data reporting. European Commission, European Research Area. Brussels. 258 p.</p>
<p><strong>BCO-DMO Data Manager Processing Notes:</strong><br />
* added a conventional header with dataset name, PI name, version date<br />
* modified parameter names to conform with BCO-DMO naming conventions<br />
* calcification values rounded to three decimal places</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
YSI Pro 2030
YSI Pro 2030
PI Supplied Instrument Name: YSI Pro 2030 PI Supplied Instrument Description:temperature measured with YSI Pro 2030 Instrument Name: YSI Professional Plus Multi-Parameter Probe Instrument Short Name:YSI ProPlus Instrument Description: The YSI Professional Plus handheld multiparameter meter provides for the measurement of a variety of combinations for dissolved oxygen, conductivity, specific conductance, salinity, resistivity, total dissolved solids (TDS), pH, ORP, pH/ORP combination, ammonium (ammonia), nitrate, chloride and temperature. More information from the manufacturer.
Mettler Toledo SevenGo Pro
Mettler Toledo SevenGo Pro
PI Supplied Instrument Name: Mettler Toledo SevenGo Pro Instrument Name: pH Sensor Instrument Short Name:pH Sensor Instrument Description: An instrument that measures the hydrogen ion activity in solutions.
The overall concentration of hydrogen ions is inversely related to its pH. The pH scale ranges from 0 to 14 and indicates whether acidic (more H+) or basic (less H+).
Metrohm 807 Titrando
Metrohm 807 Titrando
PI Supplied Instrument Name: Metrohm 807 Titrando Instrument Name: Automatic titrator Instrument Short Name:Automatic titrator Instrument Description: Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/
Deployment: Muller_Looe_Key_Reef_Acropora
Muller_Looe_Key_Reef_Acropora
Mote Offshore Coral Nursery
island
Muller_Looe_Key_Reef_Acropora
Erinn Muller
Mote Marine Laboratory
Mote Offshore Coral Nursery
island