Attributes { s { Hours { Float32 _FillValue NaN; Float32 actual_range 0.0, 64.58; String bcodmo_name "duration"; String description "Time since phyto feeding start"; String long_name "Hours"; String units "hours"; } Phyto_15N { Byte _FillValue 127; Byte actual_range 45, 45; String bcodmo_name "unknown"; String description "15N enrichment of phytoplankton at% 15N"; String long_name "Phyto 15 N"; String units "percent"; } O1_15N2 { Float32 _FillValue NaN; Float32 actual_range 0.5, 23.3; String bcodmo_name "N"; String description "Oyster mecosm1 15N enrichment of dissolved N2"; String long_name "O1 15 N2"; String units "permil (/ml)"; } O1_N2O { Float32 _FillValue NaN; Float32 actual_range 19.3, 39.8; String bcodmo_name "Nitrous Oxide"; String description "Oyster mecosm1 Aqueous N2O concentration"; String long_name "O1 N2 O"; String units "nanomole (nM)"; } O2_15N2 { Float32 _FillValue NaN; Float32 actual_range 0.4, 44.7; String bcodmo_name "N"; String description "Oyster mecosm 2 15N enrichment of dissolved N2"; String long_name "O2 15 N2"; String units "permil (/ml)"; } O2_N2O { Float32 _FillValue NaN; Float32 actual_range 30.1, 48.8; String bcodmo_name "Nitrous Oxide"; String description "Oyster mecosm 2 Aqueous N2O concentration"; String long_name "O2 N2 O"; String units "nanomole (nM)"; } O3_15N2 { Float32 _FillValue NaN; Float32 actual_range 0.4, 34.3; String bcodmo_name "N"; String description "Oyster mecosm 3 15N enrichment of dissolved N2"; String long_name "O3 15 N2"; String units "permil (/ml)"; } O3_N2O { Float32 _FillValue NaN; Float32 actual_range 14.7, 34.0; String bcodmo_name "Nitrous Oxide"; String description "Oyster mecosm 3 Aqueous N2O concentration"; String long_name "O3 N2 O"; String units "nanomole (nM)"; } C1_15N2 { Float32 _FillValue NaN; Float32 actual_range 0.4, 25.0; String bcodmo_name "N"; String description "Control mecosm 1 15N enrichment of dissolved N2"; String long_name "C1 15 N2"; String units "permil (/ml)"; } C1_N2O { Float32 _FillValue NaN; Float32 actual_range 14.6, 37.2; String bcodmo_name "Nitrous Oxide"; String description "Control mecosm 1 Aqueous N2O concentration"; String long_name "C1 N2 O"; String units "nanomole (nM)"; } C2_15N2 { Float32 _FillValue NaN; Float32 actual_range 0.2, 24.7; String bcodmo_name "N"; String description "Control mecosm 2 15N enrichment of dissolved N2"; String long_name "C2 15 N2"; String units "permil (/ml)"; } C2_N2O { Float32 _FillValue NaN; Float32 actual_range 15.5, 30.9; String bcodmo_name "Nitrous Oxide"; String description "Control mecosm 2 Aqueous N2O concentration"; String long_name "C2 N2 O"; String units "nanomole (nM)"; } C3_15N2 { Float32 _FillValue NaN; Float32 actual_range 0.3, 20.6; String bcodmo_name "N"; String description "Control mecosm 3 15N enrichment of dissolved N2"; String long_name "C3 15 N2"; String units "permil (/ml)"; } C3_N2O { Float32 _FillValue NaN; Float32 actual_range 29.2, 67.2; String bcodmo_name "Nitrous Oxide"; String description "Control mecosm 3 Aqueous N2O concentration"; String long_name "C3 N2 O"; String units "nanomole (nM)"; } } NC_GLOBAL { String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv"; String acquisition_description "15N labeled phytoplankton was continuously pumped for three days into 30.5 cm diameter mesocosms containing\\u00a0 10 cm of sediment and 20 liters of overlying water. Three mesocosms contained oysters twelve 7-cm long oysters (O1, O2, O2) and three mesocosms had no oysters and served as controls (C1,C2,C3). Mesoscosms were flow through with a 0.3 day residence time and open to the atmosphere. N2O and\\u00a015N2\\u00a0were measured during the phytoplankton addition period. Water samples for N2O analysis were collected with a peristaltic pump through a syringe needle directly into 12 ml exetainer that had been flushed with N2 and preserved with KOH to a pH above 12. Approximately six ml sample was collected. N2O concentrations in the headspace were measured on a GC- ECD.\\u00a0 Water samples for 15N2 samples were collected with a peristaltic pump through a syringe needle directly into 30 ml serum bottles that had been flushed with He and preserved with KOH to a pH above 12. \\u00a0 Approximately eight ml sample was collected. 15N2 was analyzed by GC - Isotope Ratio Mass Spectrometry (IRMS). 15N-Phyto sample was collected by GFF 0.7um filtration of phytoplankton stock solution."; String awards_0_award_nid "568863"; String awards_0_award_number "OCE-1233372"; String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1233372"; String awards_0_funder_name "NSF Division of Ocean Sciences"; String awards_0_funding_acronym "NSF OCE"; String awards_0_funding_source_nid "355"; String awards_0_program_manager "David L. Garrison"; String awards_0_program_manager_nid "50534"; String cdm_data_type "Other"; String comment "Open Mesocosm Oyster Experiment 15N2 and N2O PI: Craig Tobias Co-PI: Bongkuen Song, Michael Piehler, Mark Brush Version: 20171231"; String Conventions "COARDS, CF-1.6, ACDD-1.3"; String creator_email "info@bco-dmo.org"; String creator_name "BCO-DMO"; String creator_type "institution"; String creator_url "https://www.bco-dmo.org/"; String data_source "extract_data_as_tsv version 2.3 19 Dec 2019"; String date_created "2018-01-05T18:50:20Z"; String date_modified "2019-05-07T17:57:33Z"; String defaultDataQuery "&time<now"; String doi "10.1575/1912/bco-dmo.722433.1"; String history "2024-03-29T14:12:34Z (local files) 2024-03-29T14:12:34Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_722433.das"; String infoUrl "https://www.bco-dmo.org/dataset/722433"; String institution "BCO-DMO"; String instruments_0_acronym "IR Mass Spec"; String instruments_0_dataset_instrument_description "15N2 was measured on a Thermo Delta V IRMS fitted with a Gas Bench II interface following separation from O2 and Ar on a mol sieve 5A column."; String instruments_0_dataset_instrument_nid "722450"; String instruments_0_description "The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer)."; String instruments_0_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB16/"; String instruments_0_instrument_name "Isotope-ratio Mass Spectrometer"; String instruments_0_instrument_nid "469"; String instruments_0_supplied_name "Thermo Delta V IRMS"; String instruments_1_acronym "Gas Chromatograph"; String instruments_1_dataset_instrument_description "N2O was measured on a Agilent 7890B GC with a Poropak Column."; String instruments_1_dataset_instrument_nid "722452"; String instruments_1_description "Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)"; String instruments_1_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB02/"; String instruments_1_instrument_name "Gas Chromatograph"; String instruments_1_instrument_nid "661"; String instruments_1_supplied_name "Agilent 7890B GC with a Poropak Column"; String instruments_2_dataset_instrument_description "15N Phyto sample  was analyzed on the IRMS coupled to a Costech Elemental Analyzer."; String instruments_2_dataset_instrument_nid "722451"; String instruments_2_description "Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material."; String instruments_2_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB01/"; String instruments_2_instrument_name "Elemental Analyzer"; String instruments_2_instrument_nid "546339"; String instruments_2_supplied_name "Costech Elemental Analyzer"; String keywords "bco, bco-dmo, biological, C1_15N2, C1_N2O, C2_15N2, C2_N2O, C3_15N2, C3_N2O, chemical, data, dataset, dmo, erddap, hours, management, O1_15N2, O1_N2O, O2, O2_15N2, O2_N2O, O3_15N2, O3_N2O, oceanography, office, oxygen, phyto, Phyto_15N, preliminary"; String license "https://www.bco-dmo.org/dataset/722433/license"; String metadata_source "https://www.bco-dmo.org/api/dataset/722433"; String param_mapping "{'722433': {}}"; String parameter_source "https://www.bco-dmo.org/mapserver/dataset/722433/parameters"; String people_0_affiliation "University of Connecticut"; String people_0_affiliation_acronym "UConn - Avery Point"; String people_0_person_name "Craig Tobias"; String people_0_person_nid "51731"; String people_0_role "Principal Investigator"; String people_0_role_type "originator"; String people_1_affiliation "Virginia Institute of Marine Science"; String people_1_affiliation_acronym "VIMS"; String people_1_person_name "Mark J. Brush"; String people_1_person_nid "568861"; String people_1_role "Co-Principal Investigator"; String people_1_role_type "originator"; String people_2_affiliation "University of North Carolina at Chapel Hill"; String people_2_affiliation_acronym "UNC-Chapel Hill-IMS"; String people_2_person_name "Michael F. Piehler"; String people_2_person_nid "522931"; String people_2_role "Co-Principal Investigator"; String people_2_role_type "originator"; String people_3_affiliation "Virginia Institute of Marine Science"; String people_3_affiliation_acronym "VIMS"; String people_3_person_name "Bongkeun Song"; String people_3_person_nid "51729"; String people_3_role "Co-Principal Investigator"; String people_3_role_type "originator"; String people_4_affiliation "University of Connecticut"; String people_4_affiliation_acronym "UConn - Avery Point"; String people_4_person_name "Craig Tobias"; String people_4_person_nid "51731"; String people_4_role "Contact"; String people_4_role_type "related"; String people_5_affiliation "Woods Hole Oceanographic Institution"; String people_5_affiliation_acronym "WHOI BCO-DMO"; String people_5_person_name "Mathew Biddle"; String people_5_person_nid "708682"; String people_5_role "BCO-DMO Data Manager"; String people_5_role_type "related"; String project "Oyster Reef N2O Emission"; String projects_0_acronym "Oyster Reef N2O Emission"; String projects_0_description "Extracted from the NSF award abstract: Oyster reefs are biogeochemical hot spots and prominent estuarine habitats that provide disproportionate ecological function. Suspension-feeding eastern oysters, Crassostrea virginica, are capable of improving water quality and diminishing eutrophication by filtering nutrients and particles from the water and depositing them in the sediments. Remineralization of these deposits may enhance sedimentary denitrification that facilitates nitrogen removal in tidal estuaries. However, the scientific underpinning of oyster reef function has been challenged in various studies. In addition, recent studies of filter feeding invertebrates reported the production of nitrous oxide (N2O), a greenhouse gas, as an end product of incomplete denitrification by gut microbes. C. virginica could be another source of N2O flux from intertidal habitats. Preliminary work indicated substantial N2O production from individual oysters. The estimated N2O production from high density oyster reefs may exceed the N2O flux measured from some estuaries. With the new discovery of N2O emission and uncertainty regarding eutrophication control, the ecological value of oyster reef restoration may become equivocal. This project will quantify N2O fluxes to understand the factors controlling N2O emission from oyster reefs. Sedimentary N processes will be examined to develop an oyster reef N model to estimate N2O emission from tidal creek estuaries relative to other N cycling processes. The PIs hypothesize that intertidal oyster reefs are a substantial source of N2O emission from estuarine ecosystems and the magnitude of emission may be linked to water quality. If substantial N2O flux from oyster reefs is validated, ecological benefits of oyster reef restoration should be reevaluated. This interdisciplinary research team includes a microbial ecologist, a biogeochemist, an ecologist and an ecosystem modeler. They will utilize stable isotope and molecular microbiological techniques to quantify oyster N2O production, elucidate microbial sources of N2O emission from oysters and sediments, and estimate seasonal variation of N2O fluxes from oyster reefs. Measurements from this study will be integrated into a coupled oyster bioenergetics-sediment biogeochemistry model to compare system level rates of N cycling on oyster reefs as a function of oyster density and water quality. Modeling results will be used to assess the relative trade-­offs of oyster restoration associated with N cycling. They expect to deliver the following end products:1) estimation of annual N2O flux from oyster reefs as an additional source of greenhouse gases from estuaries, 2) a better understanding of the environmental and microbial factors influencing N2O and N2 fluxes in tidal estuaries, 3) transformative knowledge for the effect of oyster restoration on water quality enhancement and ecosystem function, 4) direct guidance for oyster restoration projects whose goals include water quality enhancement, and 5) a modeling tool for use in research and restoration planning."; String projects_0_end_date "2015-08"; String projects_0_name "Microbial Regulation of Greenhouse Gas N2O Emission from Intertidal Oyster Reefs"; String projects_0_project_nid "527289"; String projects_0_start_date "2012-12"; String publisher_name "Biological and Chemical Oceanographic Data Management Office (BCO-DMO)"; String publisher_type "institution"; String sourceUrl "(local files)"; String standard_name_vocabulary "CF Standard Name Table v55"; String subsetVariables "Phyto_15N"; String summary "Open Mesocosm Oyster 15N2 and N2O"; String title "Open Mesocosm Oyster 15N2 and N2O"; String version "1"; String xml_source "osprey2erddap.update_xml() v1.3"; } }