Attributes { s { cruise_id { String bcodmo_name "cruise_id"; String description "cruise identification number"; String long_name "Cruise Id"; String units "unitless"; } cast { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 22; String bcodmo_name "cast"; String description "cast number"; String long_name "Cast"; String units "unitless"; } date { Int32 _FillValue 2147483647; Int32 actual_range 20100807, 20120909; String bcodmo_name "date"; String description "Date sampling began"; String long_name "Date"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/"; String units "unitless"; } year { Int16 _FillValue 32767; Int16 actual_range 2010, 2012; String bcodmo_name "year"; String description "Year of sample"; String long_name "Year"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/YEARXXXX/"; String units "unitless"; } month { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 10; String bcodmo_name "month"; String description "month of sample"; String long_name "Month"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/MNTHXXXX/"; String units "unitless"; } day { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 2, 25; String bcodmo_name "day"; String description "day of sample"; String long_name "Day"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/DAYXXXXX/"; String units "unitless"; } latitude { String _CoordinateAxisType "Lat"; Float64 _FillValue NaN; Float64 actual_range 21.446917, 24.0037; String axis "Y"; String bcodmo_name "latitude"; Float64 colorBarMaximum 90.0; Float64 colorBarMinimum -90.0; String description "latitude; negative denotes South"; String ioos_category "Location"; String long_name "Latitude"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P09/current/LATX/"; String standard_name "latitude"; String units "degrees_north"; } longitude { String _CoordinateAxisType "Lon"; Float64 _FillValue NaN; Float64 actual_range -158.311817, -157.96755; String axis "X"; String bcodmo_name "longitude"; Float64 colorBarMaximum 180.0; Float64 colorBarMinimum -180.0; String description "longitude; negative denotes West"; String ioos_category "Location"; String long_name "Longitude"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P09/current/LONX/"; String standard_name "longitude"; String units "degrees_east"; } depth { String _CoordinateAxisType "Height"; String _CoordinateZisPositive "down"; Float64 _FillValue NaN; Float64 actual_range 5.0, 25.0; String axis "Z"; String bcodmo_name "depth"; Float64 colorBarMaximum 8000.0; Float64 colorBarMinimum -8000.0; String colorBarPalette "TopographyDepth"; String description "depth from which sample was collected"; String ioos_category "Location"; String long_name "Depth"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P09/current/DEPH/"; String positive "down"; String standard_name "depth"; String units "m"; } PP_mean_gt_0pt2um { Float32 _FillValue NaN; Float32 actual_range 0.03, 0.6; String bcodmo_name "Primary Production"; String description "mean 14C-Primary Production rate from 0.2 micron filters"; String long_name "PP Mean Gt 0pt2um"; String units "micromol C/liter/day"; } PP_std_dev_gt_0pt2um { Float32 _FillValue NaN; Float32 actual_range 0.0, 0.28; String bcodmo_name "Primary Production"; String description "standard deviation of 14C-Primary Production rate from 0.2 micron filters"; String long_name "PP Std Dev Gt 0pt2um"; String units "micromol C/liter/day"; } PP_num_obs_gt_0pt2um { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of PP rate mean and standard deviation"; String long_name "PP Num Obs Gt 0pt2um"; String units "unitless"; } PP_mean_750uatm_pco2_gt_0pt2um { Float32 _FillValue NaN; Float32 actual_range 0.41, 0.46; String bcodmo_name "Primary Production"; String description "mean 14C-Primary Production rate from 0.2 micron filters, incubated at 750 microatm pCO2"; String long_name "PP Mean 750uatm Pco2 Gt 0pt2um"; String units "micromol C/liter/day"; } PP_std_dev_750uatm_pco2_gt_0pt2um { Float32 _FillValue NaN; Float32 actual_range 0.03, 0.05; String bcodmo_name "Primary Production"; String description "standard deviation of 14C-Primary Production rate from 0.2 micron filters, incubated at 750 microatm pCO2"; String long_name "PP Std Dev 750uatm Pco2 Gt 0pt2um"; String units "micromol C/liter/day"; } PP_num_obs_750uatm_pco2_gt_0pt2um { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 2, 2; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of PP rate mean and standard deviation"; String long_name "PP Num Obs 750uatm Pco2 Gt 0pt2um"; String units "unitless"; } PP_mean_1100uatm_pco2_gt_0pt2um { Float32 _FillValue NaN; Float32 actual_range 0.07, 0.63; String bcodmo_name "Primary Production"; String description "mean 14C-Primary Production rate from 0.2 micron filters incubated at 1100 microatm pCO2"; String long_name "PP Mean 1100uatm Pco2 Gt 0pt2um"; String units "micromol C/liter/day"; } PP_std_dev_1100uatm_pco2_gt_0pt2um { Float32 _FillValue NaN; Float32 actual_range 0.0, 0.41; String bcodmo_name "standard deviation"; String description "standard deviation of 14C-Primary Production rate from 0.2 micron filters incubated at 1100 microatm pCO2"; String long_name "PP Std Dev 1100uatm Pco2 Gt 0pt2um"; String units "micromol C/liter/day"; } PP_num_obs_1100uatm_pco2_gt_0pt2um { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of PP rate mean and standard deviation"; String long_name "PP Num Obs 1100uatm Pco2 Gt 0pt2um"; String units "unitless"; } dissolved_inorganic_carbon_mean { Float32 _FillValue NaN; Float32 actual_range 1974.55, 2198.1; String bcodmo_name "DIC"; String description "mean dissolved inorganic carbon of seawater used for experimental control conditions"; String long_name "Dissolved Inorganic Carbon Mean"; String units "micromol/kilogram seawater"; } dissolved_inorganic_carbon_stdev { Float32 _FillValue NaN; Float32 actual_range 0.25, 22.22; String bcodmo_name "standard deviation"; String description "standard deviation of dissolved inorganic carbon of seawater used for experimental control conditions"; String long_name "Dissolved Inorganic Carbon Stdev"; String units "micromol/kilogram seawater"; } dissolved_inorganic_carbon_num_obs { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of dissolved inorganic carbon"; String long_name "Dissolved Inorganic Carbon Num Obs"; String units "unitless"; } dissolved_inorganic_carbon_750uatm_pco2_mean { Float32 _FillValue NaN; Float32 actual_range 2130.66, 2130.95; String bcodmo_name "DIC"; String description "mean dissolved inorganic carbon of seawater used for experimental 750 microatm pCO2 treatments"; String long_name "Dissolved Inorganic Carbon 750uatm Pco2 Mean"; String units "micromol/kilogram seawater"; } dissolved_inorganic_carbon_750uatm_pco2_stdev { Float32 _FillValue NaN; Float32 actual_range 0.01, 1.79; String bcodmo_name "standard deviation"; String description "standard deviation of dissolved inorganic carbon of seawater used for experimental 750 microatm pCO2 treatments"; String long_name "Dissolved Inorganic Carbon 750uatm Pco2 Stdev"; String units "micromol/kilogram seawater"; } dissolved_inorganic_carbon_750uatm_pco2_num_obs { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 2, 2; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of dissolved inorganic carbon"; String long_name "Dissolved Inorganic Carbon 750uatm Pco2 Num Obs"; String units "unitless"; } dissolved_inorganic_carbon_1100uatm_pco2_mean { Float32 _FillValue NaN; Float32 actual_range 2000.92, 2244.73; String bcodmo_name "DIC"; String description "mean dissolved inorganic carbon of seawater used for experimental 1100 microatm pCO2 treatments"; String long_name "Dissolved Inorganic Carbon 1100uatm Pco2 Mean"; String units "micromol/kilogram seawater"; } dissolved_inorganic_carbon_1100uatm_pco2_stdev { Float32 _FillValue NaN; Float32 actual_range 0.19, 9.8; String bcodmo_name "standard deviation"; String description "standard deviation of dissolved inorganic carbon of seawater used for experimental 1100 microatm pCO2 treatments"; String long_name "Dissolved Inorganic Carbon 1100uatm Pco2 Stdev"; String units "micromol/kilogram seawater"; } dissolved_inorganic_carbon_1100uatm_pco2_num_obs { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of dissolved inorganic carbon"; String long_name "Dissolved Inorganic Carbon 1100uatm Pco2 Num Obs"; String units "unitless"; } total_alkalinity_mean { Float32 _FillValue NaN; Float32 actual_range 2291.13, 2348.7; String bcodmo_name "TALK"; String description "mean total alkalinity of seawater used for experimental control conditions"; String long_name "Total Alkalinity Mean"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/MDMAP014/"; String units "microequivalents/kilogram seawater"; } total_alkalinity_stdev { Float32 _FillValue NaN; Float32 actual_range 0.06, 27.27; String bcodmo_name "standard deviation"; String description "standard deviation of total alkalinity of seawater used for experimental control conditions"; String long_name "Total Alkalinity Stdev"; String units "microequivalents/kilogram seawater"; } total_alkalinity_num_obs { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used in total alkalinity"; String long_name "Total Alkalinity Num Obs"; String units "unitless"; } total_alkalinity_750uatm_pco2_mean { Float32 _FillValue NaN; Float32 actual_range 2314.03, 2319.76; String bcodmo_name "TALK"; String description "mean total alkalinity of seawater used for experimental 750 microatm pCO2 treatments"; String long_name "Total Alkalinity 750uatm Pco2 Mean"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/MDMAP014/"; String units "microequivalents/kilogram seawater"; } total_alkalinity_750uatm_pco2_stdev { Float32 _FillValue NaN; Float32 actual_range 0.51, 2.22; String bcodmo_name "standard deviation"; String description "standard deviation of total alkalinity of seawater used for experimental 750 microatm pCO2 treatments"; String long_name "Total Alkalinity 750uatm Pco2 Stdev"; String units "microequivalents/kilogram seawater"; } total_alkalinity_750uatm_pco2_num_obs { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 2, 2; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of total alkalinity"; String long_name "Total Alkalinity 750uatm Pco2 Num Obs"; String units "unitless"; } total_alkalinity_1100uatm_pco2_mean { Float32 _FillValue NaN; Float32 actual_range 2279.97, 2354.52; String bcodmo_name "TALK"; String description "mean total alkalinity of seawater used for experimental 1100 microatm pCO2 treatments"; String long_name "Total Alkalinity 1100uatm Pco2 Mean"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/MDMAP014/"; String units "microequivalents/kilogram seawater"; } total_alkalinity_1100uatm_pco2_stdev { Float32 _FillValue NaN; Float32 actual_range 0.26, 38.99; String bcodmo_name "standard deviation"; String description "standard deviation of total alkalinity of seawater used for experimental 1100 microatm pCO2 treatments"; String long_name "Total Alkalinity 1100uatm Pco2 Stdev"; String units "microequivalents/kilogram seawater"; } total_alkalinity_1100uatm_pco2_num_obs { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of total alkalinity"; String long_name "Total Alkalinity 1100uatm Pco2 Num Obs"; String units "unitless"; } chlorophyll { Float32 _FillValue NaN; Float32 actual_range 0.05, 0.28; String bcodmo_name "chlorophyll a"; Float64 colorBarMaximum 30.0; Float64 colorBarMinimum 0.03; String colorBarScale "Log"; String description "mean chlorophyll a in experimental controls"; String long_name "Concentration Of Chlorophyll In Sea Water"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/"; String units "micrograms/liter"; } chlorophyll_stdev { Float32 _FillValue NaN; Float32 actual_range 0.0, 0.03; String bcodmo_name "standard deviation"; Float64 colorBarMaximum 30.0; Float64 colorBarMinimum 0.03; String colorBarScale "Log"; String description "standard deviation of chlorophyll a in experimental controls"; String long_name "Concentration Of Chlorophyll In Sea Water"; String units "micrograms/liter"; } chlorophyll_num_obs { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; Float64 colorBarMaximum 30.0; Float64 colorBarMinimum 0.03; String colorBarScale "Log"; String description "number of samples used to calculate chlorophyll a mean and standard deviation"; String long_name "Concentration Of Chlorophyll In Sea Water"; String units "unitless"; } chlorophyll_750uatm_pco2 { Float32 _FillValue NaN; Float32 actual_range 0.0, 0.01; String bcodmo_name "chlorophyll a"; Float64 colorBarMaximum 30.0; Float64 colorBarMinimum 0.03; String colorBarScale "Log"; String description "mean chlorophyll a in 750 microatm pco2 treatments"; String long_name "Concentration Of Chlorophyll In Sea Water"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/"; String units "micrograms/liter"; } chlorophyll_750uatm_pco2_stdev { Float64 _FillValue NaN; String bcodmo_name "standard deviation"; Float64 colorBarMaximum 30.0; Float64 colorBarMinimum 0.03; String colorBarScale "Log"; String description "standard deviation of chlorophyll a in 750 microatm pco2 treatments"; String long_name "Concentration Of Chlorophyll In Sea Water"; String units "micrograms/liter"; } chlorophyll_750uatm_pco2_num_obs { Float32 _FillValue NaN; Float32 actual_range 1.0, 1.0; String bcodmo_name "numb_obs"; Float64 colorBarMaximum 30.0; Float64 colorBarMinimum 0.03; String colorBarScale "Log"; String description "number of samples used to calculate chlorophyll a mean and standard deviation"; String long_name "Concentration Of Chlorophyll In Sea Water"; String units "unitless"; } chorophyll_1100uatm_pco2 { Float32 _FillValue NaN; Float32 actual_range 0.06, 0.19; String bcodmo_name "chlorophyll a"; String description "mean chlorophyll a in 1100 microatm pco2 treatments"; String long_name "Chorophyll 1100uatm Pco2"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/"; String units "micrograms/liter"; } chorophyll_1100uatm_pco2_stdev { Float32 _FillValue NaN; Float32 actual_range 0.0, 0.02; String bcodmo_name "standard deviation"; String description "standard deviation of chlorophyll a in 1100 microatm pco2 treatments"; String long_name "Chorophyll 1100uatm Pco2 Stdev"; String units "micrograms/liter"; } chorophyll_1100uatm_pco2_num_obs { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used to calculate chlorophyll a mean and standard deviation"; String long_name "Chorophyll 1100uatm Pco2 Num Obs"; String units "unitless"; } leuc_3H_light_incorp_mean { Float32 _FillValue NaN; Float32 actual_range 9.24, 81.18; String bcodmo_name "leuc_incorp"; String description "mean 3H-Leucine (light incubated) incorporation rates"; String long_name "Leuc 3 H Light Incorp Mean"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/"; String units "picomol leucine/liter/hour"; } leuc_3H_light_incorp_stdev { Float32 _FillValue NaN; Float32 actual_range 0.54, 26.89; String bcodmo_name "standard deviation"; String description "standard deviation 3H-Leucine (light incubated) incorporation rates"; String long_name "Leuc 3 H Light Incorp Stdev"; String units "picomol leucine/liter/hour"; } leuc_3H_light_num_obs { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of 3H-leucine incorporation rate mean and standard deviation"; String long_name "Leuc 3 H Light Num Obs"; String units "unitless"; } leuc_3H_light_incorp_mean_750uatm_pco2 { Float32 _FillValue NaN; Float32 actual_range 26.24, 99.07; String bcodmo_name "leuc_incorp"; String description "mean 3H-Leucine (light incubated) incorporation rates at 750 microatm pCO2"; String long_name "Leuc 3 H Light Incorp Mean 750uatm Pco2"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/"; String units "picomol leucine/liter/hour"; } leuc_3H_light_incorp_std_dev_750uatm_pco2 { Float32 _FillValue NaN; Float32 actual_range 2.4, 13.34; String bcodmo_name "standard deviation"; String description "standard deviation 3H-Leucine (light incubated) incorporation rates at 750 microatm pCO2"; String long_name "Leuc 3 H Light Incorp Std Dev 750uatm Pco2"; String units "picomole leucine/liter/hour"; } leuc_3H_light_num_obs_750uatm_pco2 { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 2, 2; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of 3H-leucine incorporation rate mean and standard deviation"; String long_name "Leuc 3 H Light Num Obs 750uatm Pco2"; String units "unitless"; } leuc_3H_light_incorp_mean_1100uatm_pco2 { Float32 _FillValue NaN; Float32 actual_range 15.33, 76.99; String bcodmo_name "leuc_incorp"; String description "mean 3H-Leucine (light incubated) incorporation rates at 1100 microatm pCO2"; String long_name "Leuc 3 H Light Incorp Mean 1100uatm Pco2"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/"; String units "picomol leucine/liter/hour"; } leuc_3H_light_incorp_std_dev_1100uatm_pco2 { Float32 _FillValue NaN; Float32 actual_range 0.1, 20.39; String bcodmo_name "standard deviation"; String description "standard deviation 3H-Leucine (light incubated) incorporation rates at 1100 microatm pCO2"; String long_name "Leuc 3 H Light Incorp Std Dev 1100uatm Pco2"; String units "picomol leucine/liter/hour"; } leuc_3H_light_num_obs_1100uatm_pco2 { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of 3H-leucine incorporation rate mean and standard deviation"; String long_name "Leuc 3 H Light Num Obs 1100uatm Pco2"; String units "unitless"; } leuc_3H_dark_incorp_mean { Float32 _FillValue NaN; Float32 actual_range 7.46, 66.53; String bcodmo_name "leuc_incorp"; String description "Mean 3H-Leucine (dark incubated) incorporation rates"; String long_name "Leuc 3 H Dark Incorp Mean"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/"; String units "picomol leucine/liter/hour"; } leuc_3H_dark_incorp_std_dev { Float32 _FillValue NaN; Float32 actual_range 0.4, 17.45; String bcodmo_name "standard deviation"; String description "standard deviation 3H-Leucine (dark incubated) incorporation rates"; String long_name "Leuc 3 H Dark Incorp Std Dev"; String units "picomol leucine/liter/hour"; } leuc_3H_dark_num_obs { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of 3H-leucine incorporation rate mean and standard deviation"; String long_name "Leuc 3 H Dark Num Obs"; String units "unitless"; } leuc_3H_dark_incorp_mean_750uatm_pco2 { Float32 _FillValue NaN; Float32 actual_range 20.91, 90.33; String bcodmo_name "leuc_incorp"; String description "mean 3H-Leucine (dark incubated) incorporation rates at 750 microatm pCO2"; String long_name "Leuc 3 H Dark Incorp Mean 750uatm Pco2"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/"; String units "picomol leucine/liter/hour"; } leuc_3H_dark_incorp_std_dev_750uatm_pco2 { Float32 _FillValue NaN; Float32 actual_range 2.83, 11.4; String bcodmo_name "standard deviation"; String description "standard deviation 3H-Leucine dark incubated) incorporation rates at 750 microatm pCO2"; String long_name "Leuc 3 H Dark Incorp Std Dev 750uatm Pco2"; String units "picomol leucine/liter/hour"; } leuc_3H_dark_num_obs_750uatm_pco2 { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 2, 2; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of 3H-leucine incorporation rate mean and standard deviation"; String long_name "Leuc 3 H Dark Num Obs 750uatm Pco2"; String units "unitless"; } leuc_3H_dark_incorp_mean_1100uatm_pco2 { Float32 _FillValue NaN; Float32 actual_range 2.01, 76.99; String bcodmo_name "leuc_incorp"; String description "mean 3H-Leucine (dark incubated) incorporation rates at 1100 microatm pCO2"; String long_name "Leuc 3 H Dark Incorp Mean 1100uatm Pco2"; String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/UPLERIP4/"; String units "picomol leucine/liter/hour"; } leuc_3H_dark_incorp_std_dev_1100uatm_pco2 { Float32 _FillValue NaN; Float32 actual_range 0.38, 25.06; String bcodmo_name "standard deviation"; String description "standard deviation 3H-Leucine dark incubated) incorporation rates at 1100 microatm pCO2"; String long_name "Leuc 3 H Dark Incorp Std Dev 1100uatm Pco2"; String units "picomol leucine/liter/hour"; } leuc_3H_dark_num_obs_1100uatm_pco2 { Byte _FillValue 127; String _Unsigned "false"; Byte actual_range 1, 3; String bcodmo_name "numb_obs"; String description "number of samples used in calculation of 3H-leucine incorporation rate mean and standard deviation"; String long_name "Leuc 3 H Dark Num Obs 1100uatm Pco2"; String units "unitless"; } } NC_GLOBAL { String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson"; String acquisition_description "Rates of primary production were assessed using the 14C-bicarbonate incorporation technique. Rates of bacterial production were assessed using incorporation of 3H-leucine. Whole near-surface seawater was collected into acid-washed 20 L carboys. Control carboys were bubbled with air; treatment carboys were bubbled with a mixture of air and CO2, to increase the pCO2 to either ~750 or 1100 \\u00b5atm. Sampling of each time point was conducted before dawn, experiments lasted between 2 and 5 days. Water from each carboy was subsampled into acid washed 500 mL polycarbonate bottles for primary production rate measurements. To each bottle, was then added ~1.85 MBq 14C- bicarbonate. Water from each carboy was also collected in an opaque polyethylene amber bottles and then subsampled into six 1.5 mL microcentrifuge tubes for bacterial production rate measurements. Each tube was then inoculated with 3H-leucine to a final concentration of 20 nmol L-1. Three tubes from each carboy were incubated in the dark (in a opaque cloth bag) and three in the light. Time zero blanks were immediately subsampled from each amber bottle, by aliquoting 1.5 mL of seawater into 2 mL microcentrifuge tubes each containing 100 \\u00b5L of 100% TCA. Following addition of radioactive substrates, the primary production bottles and bacterial production tubes were placed in shaded (~50% irradiance) surface seawater-cooled incubators for the duration of the photoperiod. After sunset, the total radioactivity added to each primary production sample bottle was determined by subsampling 250 \\u00b5L aliquots of seawater into scintillation vials containing 500 \\u00b5L of \\u03b2-phenylethylamine. 100 mL from each 500 mL sample bottle was filtered at low vacuum (<50 mm Hg) onto 25 mm diameter, 0.2 porosity polycarbonate membrane filters. Filters were stored frozen in 20 mL scintillation vials until analysis. Analysis consisted of acidification via addition of 1 mL of 2 N hydrochloric acid, and passively venting at least 24 hours in a fume hood to remove all inorganic 14C. Addition of 10 mL Ultima Gold LLT liquid scintillation cocktail and counting on a Perkin Elmer 2600 liquid scintillation counter completed the primary production analysis. After sunset, 100 \\u00b5L of 100% TCA was added to each microcentrifuge tube. The microcentrifuge tubes were frozen (-20\\u00b0C) for subsequent processing, following the procedures described in Smith and Azam 1992. Samples for the determination of dissolved inorganic carbon and total alkalinity were collected from each carboy and analyzed according to the protocols of the Hawaii Ocean Time-series (Dore et al. 2009; Winn et al. 1998). DIC and TA samples were collected into precombusted 300 mL borosilicate bottles. Care was taken to avoid introduction of air bubbles into samples during filling; bottles were allowed to overflow three times during filling. Once filled, samples were immediately fixed with 100 \\u00b5L of a saturated solution of mercuric chloride; bottles were capped with a grease seal, and stored in the dark for later analysis. Samples for measurement of fluorometric chlorophyll\\u00a0a\\u00a0were collected according to the protocols of the Hawaii Ocean Time-series; analysis was performed following Letelier et al. (1996)."; String awards_0_award_nid "54679"; String awards_0_award_number "OCE-0850827"; String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0850827"; String awards_0_funder_name "NSF Division of Ocean Sciences"; String awards_0_funding_acronym "NSF OCE"; String awards_0_funding_source_nid "355"; String awards_0_program_manager "David L. Garrison"; String awards_0_program_manager_nid "50534"; String cdm_data_type "Other"; String comment "pCO2 manipulation experiments (experimental) 2010-2012 PI's: Church M., Letelier R., Viviani D. Dataset ID: 726342 Version: 1 Last updated: 2018-03-15"; String Conventions "COARDS, CF-1.6, ACDD-1.3"; String creator_email "info@bco-dmo.org"; String creator_name "BCO-DMO"; String creator_type "institution"; String creator_url "https://www.bco-dmo.org/"; String data_source "extract_data_as_tsv version 2.3 19 Dec 2019"; String date_created "2018-02-06T19:37:41Z"; String date_modified "2019-06-12T20:23:39Z"; String defaultDataQuery "&time<now"; String doi "10.1575/1912/bco-dmo.726342.1"; Float64 Easternmost_Easting -157.96755; Float64 geospatial_lat_max 24.0037; Float64 geospatial_lat_min 21.446917; String geospatial_lat_units "degrees_north"; Float64 geospatial_lon_max -157.96755; Float64 geospatial_lon_min -158.311817; String geospatial_lon_units "degrees_east"; Float64 geospatial_vertical_max 25.0; Float64 geospatial_vertical_min 5.0; String geospatial_vertical_positive "down"; String geospatial_vertical_units "m"; String history "2024-11-08T09:14:47Z (local files) 2024-11-08T09:14:47Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_726342.das"; String infoUrl "https://www.bco-dmo.org/dataset/726342"; String institution "BCO-DMO"; String instruments_0_acronym "CTD"; String instruments_0_dataset_instrument_nid "732002"; String instruments_0_description "The Conductivity, Temperature, Depth (CTD) unit is an integrated instrument package designed to measure the conductivity, temperature, and pressure (depth) of the water column. The instrument is lowered via cable through the water column and permits scientists observe the physical properties in real time via a conducting cable connecting the CTD to a deck unit and computer on the ship. The CTD is often configured with additional optional sensors including fluorometers, transmissometers and/or radiometers. It is often combined with a Rosette of water sampling bottles (e.g. Niskin, GO-FLO) for collecting discrete water samples during the cast. This instrument designation is used when specific make and model are not known."; String instruments_0_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/130/"; String instruments_0_instrument_name "CTD profiler"; String instruments_0_instrument_nid "417"; String instruments_1_acronym "LSC"; String instruments_1_dataset_instrument_nid "730942"; String instruments_1_description "Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used the quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples."; String instruments_1_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB21/"; String instruments_1_instrument_name "Liquid Scintillation Counter"; String instruments_1_instrument_nid "624"; String instruments_1_supplied_name "Perkin Elmer 2600 liquid scintillation counter"; String keywords "0pt2um, 1100uatm, 750uatm, alkalinity, bco, bco-dmo, biological, carbon, cast, chemical, chemistry, chlorophyll, chlorophyll_750uatm_pco2, chlorophyll_750uatm_pco2_num_obs, chlorophyll_750uatm_pco2_stdev, chlorophyll_num_obs, chlorophyll_stdev, chorophyll, chorophyll_1100uatm_pco2, chorophyll_1100uatm_pco2_num_obs, chorophyll_1100uatm_pco2_stdev, concentration, concentration_of_chlorophyll_in_sea_water, cruise, cruise_id, dark, data, dataset, date, day, depth, dev, deviation, dissolved, dissolved_inorganic_carbon_1100uatm_pco2_mean, dissolved_inorganic_carbon_1100uatm_pco2_num_obs, dissolved_inorganic_carbon_1100uatm_pco2_stdev, dissolved_inorganic_carbon_750uatm_pco2_mean, dissolved_inorganic_carbon_750uatm_pco2_num_obs, dissolved_inorganic_carbon_750uatm_pco2_stdev, dissolved_inorganic_carbon_mean, dissolved_inorganic_carbon_num_obs, dissolved_inorganic_carbon_stdev, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Chlorophyll, erddap, incorp, inorganic, latitude, leuc, leuc_3H_dark_incorp_mean, leuc_3H_dark_incorp_mean_1100uatm_pco2, leuc_3H_dark_incorp_mean_750uatm_pco2, leuc_3H_dark_incorp_std_dev, leuc_3H_dark_incorp_std_dev_1100uatm_pco2, leuc_3H_dark_incorp_std_dev_750uatm_pco2, leuc_3H_dark_num_obs, leuc_3H_dark_num_obs_1100uatm_pco2, leuc_3H_dark_num_obs_750uatm_pco2, leuc_3H_light_incorp_mean, leuc_3H_light_incorp_mean_1100uatm_pco2, leuc_3H_light_incorp_mean_750uatm_pco2, leuc_3H_light_incorp_std_dev_1100uatm_pco2, leuc_3H_light_incorp_std_dev_750uatm_pco2, leuc_3H_light_incorp_stdev, leuc_3H_light_num_obs, leuc_3H_light_num_obs_1100uatm_pco2, leuc_3H_light_num_obs_750uatm_pco2, light, longitude, management, mean, month, num, obs, observations, ocean, oceanography, oceans, office, pco2, PP_mean_1100uatm_pco2_gt_0pt2um, PP_mean_750uatm_pco2_gt_0pt2um, PP_mean_gt_0pt2um, PP_num_obs_1100uatm_pco2_gt_0pt2um, PP_num_obs_750uatm_pco2_gt_0pt2um, PP_num_obs_gt_0pt2um, PP_std_dev_1100uatm_pco2_gt_0pt2um, PP_std_dev_750uatm_pco2_gt_0pt2um, PP_std_dev_gt_0pt2um, preliminary, profiler, salinity, salinity-temperature-depth, science, sea, seawater, standard, standard deviation, std, stdev, temperature, total, total_alkalinity_1100uatm_pco2_mean, total_alkalinity_1100uatm_pco2_num_obs, total_alkalinity_1100uatm_pco2_stdev, total_alkalinity_750uatm_pco2_mean, total_alkalinity_750uatm_pco2_num_obs, total_alkalinity_750uatm_pco2_stdev, total_alkalinity_mean, total_alkalinity_num_obs, total_alkalinity_stdev, water, year"; String keywords_vocabulary "GCMD Science Keywords"; String license "https://www.bco-dmo.org/dataset/726342/license"; String metadata_source "https://www.bco-dmo.org/api/dataset/726342"; Float64 Northernmost_Northing 24.0037; String param_mapping "{'726342': {'lat': 'master - latitude', 'depth': 'flag - depth', 'lon': 'master - longitude'}}"; String parameter_source "https://www.bco-dmo.org/mapserver/dataset/726342/parameters"; String people_0_affiliation "University of Hawaii"; String people_0_person_name "Matthew J. Church"; String people_0_person_nid "50675"; String people_0_role "Principal Investigator"; String people_0_role_type "originator"; String people_1_affiliation "Oregon State University"; String people_1_affiliation_acronym "OSU-CEOAS"; String people_1_person_name "Dr Ricardo Letelier"; String people_1_person_nid "50769"; String people_1_role "Co-Principal Investigator"; String people_1_role_type "originator"; String people_2_affiliation "University of Hawaii"; String people_2_person_name "Donn Viviani"; String people_2_person_nid "727509"; String people_2_role "Contact"; String people_2_role_type "related"; String people_3_affiliation "Woods Hole Oceanographic Institution"; String people_3_affiliation_acronym "WHOI BCO-DMO"; String people_3_person_name "Megan Switzer"; String people_3_person_nid "708683"; String people_3_role "BCO-DMO Data Manager"; String people_3_role_type "related"; String project "DIAZOTROPHS-CO2"; String projects_0_acronym "DIAZOTROPHS-CO2"; String projects_0_description "The North Pacific Subtropical Gyre (NPSG) is the largest ocean ecosystem on Earth, playing a prominent role in global carbon cycling and forming an important reservoir of marine biodiversity. Nitrogen (N2) fixing bacteria (termed diazotrophs) provide a major source of new nitrogen to the oligotrophic waters of the NPSG, thereby exerting direct control on the carbon cycle. Oceanic uptake of CO2 causes long-term changes in the partial pressure of CO2 (pCO2) in the seawater of this ecosystem. Therefore, understanding how carbon system perturbations may influence ocean biogeochemistry is an important and timely undertaking. In this project, the investigators will examine how natural assemblages of N2 fixing microorganisms respond to perturbations in seawater carbon chemistry. Laboratory and field-based experiments will be placed in the context of monthly time series measurements on the activities and abundances of N2 fixing microorganism abundances. Together, the project will provide insight into the dependence of N2 fixing microorganism physiology on variations in CO2. The broad objectives of the research are: (1) Quantify the responses and consequences of changes in seawater pCO2 on the growth and community structure of naturally-occurring assemblages of ocean diazotrophs; (2) Identify why and how changes in seawater pCO2 influence the growth and carbon acquisition strategies of two model marine diazotrophs (Trichodesmium and Crocosphaera); and (3) Quantify temporal variability in diazotroph community structure and activities at Station ALOHA. This is a Collaborative Research award."; String projects_0_end_date "2012-06"; String projects_0_name "Oceanic diazotroph community structure and activities in a high carbon dioxide world"; String projects_0_project_nid "2102"; String projects_0_start_date "2009-07"; String publisher_name "Biological and Chemical Oceanographic Data Management Office (BCO-DMO)"; String publisher_type "institution"; String sourceUrl "(local files)"; Float64 Southernmost_Northing 21.446917; String standard_name_vocabulary "CF Standard Name Table v55"; String subsetVariables "chlorophyll_750uatm_pco2_stdev"; String summary "Rates of primary and bacterial production, and chlorophyll concentrations measured experimentally under ambient and elevated pCO2 (750 or 1100 \\u00b5atm) from Hawaii Ocean Time-series near Station ALOHA from 2010-2011."; String title "[Experimental pCO2 manipulation experiments 2010-2012] - Rates of primary and bacterial production, and chlorophyll concentrations measured experimentally under ambient and elevated pCO2 (750 or 1100 µatm) from Hawaii Ocean Time-series near Station ALOHA from 2010-2011. (Oceanic diazotroph community structure and activities in a high carbon dioxide world)"; String version "1"; Float64 Westernmost_Easting -158.311817; String xml_source "osprey2erddap.update_xml() v1.3"; } }