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Dataset Title:	[Concentrations of phospholipid-linked fatty acids (PLFAs)] - Concentrations of phospholipid-linked fatty acids (PLFAs) in the surface sediments of three marsh ponds in PIE-LTER (Rowley, MA) from 2014. (Eutrophication Effects on Sediment Metabolism and Benthic Algal-bacterial Coupling: An Application of Novel Techniques in a LTER Estuary)
Institution:	BCO-DMO (Dataset ID: bcodmo_dataset_738160)
Information:	Summary \| License \| ISO 19115 \| Metadata \| Background \| Data Access Form \| Files

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Anyone can use ERDDAPs Slide Sorter to build a personal web page that displays graphs with the latest data (or other images or HTML content), each in its own, draggable slide.

The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  Pond {
    Byte _FillValue 127;
    String _Unsigned "false";
    Byte actual_range 1, 3;
    String bcodmo_name "Site_ID";
    String description "Site number";
    String long_name "Pond";
    String units "unitless";
  }
  Week {
    Byte _FillValue 127;
    String _Unsigned "false";
    Byte actual_range 1, 11;
    String bcodmo_name "time_point";
    String description "Week number";
    String long_name "Week";
    String units "unitless";
  }
  Season {
    String bcodmo_name "season";
    String description "Season at time of sampling";
    String long_name "Season";
    String units "unitless";
  }
  Tide {
    String bcodmo_name "unknown";
    String description "Tide type";
    String long_name "Tide";
    String units "unitless";
  }
  Sampling_Date {
    String bcodmo_name "date";
    String description "Sampling date; yyyy/mm/dd";
    String long_name "Sampling Date";
    String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/";
    String source_name "Sampling_Date";
    String time_precision "1970-01-01";
    String units "unitless";
  }
  C12_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.08, 0.45;
    String bcodmo_name "unknown";
    String description "Concentration C12 0";
    String long_name "C12 0";
    String units "per mil";
  }
  iso_C13 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.04, 0.16;
    String bcodmo_name "unknown";
    String description "Concentration iso C13";
    String long_name "Iso C13";
    String units "per mil";
  }
  anteiso_C13 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.05, 0.13;
    String bcodmo_name "unknown";
    String description "Concentration anteiso C13";
    String long_name "Anteiso C13";
    String units "per mil";
  }
  C13_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.01, 0.17;
    String bcodmo_name "unknown";
    String description "Concentration C13 0";
    String long_name "C13 0";
    String units "per mil";
  }
  iso_C14 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.57, 1.23;
    String bcodmo_name "unknown";
    String description "Concentration iso C14";
    String long_name "Iso C14";
    String units "per mil";
  }
  C14_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 2.19, 3.51;
    String bcodmo_name "unknown";
    String description "Concentration C14 0";
    String long_name "C14 0";
    String units "per mil";
  }
  iso_C15 {
    Float32 _FillValue NaN;
    Float32 actual_range 2.21, 4.13;
    String bcodmo_name "unknown";
    String description "Concentration iso C15";
    String long_name "Iso C15";
    String units "per mil";
  }
  anteiso_C15 {
    Float32 _FillValue NaN;
    Float32 actual_range 2.5, 5.4;
    String bcodmo_name "unknown";
    String description "Concentration anteiso C15";
    String long_name "Anteiso C15";
    String units "per mil";
  }
  C15_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 1.24, 3.06;
    String bcodmo_name "unknown";
    String description "Concentration C15 0";
    String long_name "C15 0";
    String units "per mil";
  }
  iso_C16 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 2.01;
    String bcodmo_name "unknown";
    String description "Concentration iso C16";
    String long_name "Iso C16";
    String units "per mil";
  }
  iso_16_1w9c {
    Float32 _FillValue NaN;
    Float32 actual_range 9.21, 13.6;
    String bcodmo_name "unknown";
    String description "Concentration iso 16 1w9c";
    String long_name "Iso 16 1w9c";
    String units "per mil";
  }
  iso_16_1w9t {
    Float32 _FillValue NaN;
    Float32 actual_range 1.29, 2.14;
    String bcodmo_name "unknown";
    String description "Concentration iso 16 1w9t";
    String long_name "Iso 16 1w9t";
    String units "per mil";
  }
  iso_16_1w7 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.51, 1.42;
    String bcodmo_name "unknown";
    String description "Concentration iso 16 1w7";
    String long_name "Iso 16 1w7";
    String units "per mil";
  }
  C16_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 17.29, 23.12;
    String bcodmo_name "unknown";
    String description "Concentration C16 0";
    String long_name "C16 0";
    String units "per mil";
  }
  methyl_10_C16 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.82, 1.63;
    String bcodmo_name "unknown";
    String description "Concentration 10 methyl C16";
    String long_name "Methyl 10 C16";
    String units "per mil";
  }
  iso_C17 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 1.35;
    String bcodmo_name "unknown";
    String description "Concentration C17";
    String long_name "Iso C17";
    String units "per mil";
  }
  anteiso_C17 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 1.62;
    String bcodmo_name "unknown";
    String description "Concentration anteiso C17";
    String long_name "Anteiso C17";
    String units "per mil";
  }
  C17_1 {
    Float32 _FillValue NaN;
    Float32 actual_range 1.49, 3.85;
    String bcodmo_name "unknown";
    String description "Concentration C17 1";
    String long_name "C17 1";
    String units "per mil";
  }
  C17_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 1.08, 1.75;
    String bcodmo_name "unknown";
    String description "Concentration C17 0";
    String long_name "C17 0";
    String units "per mil";
  }
  C18_4 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 2.24;
    String bcodmo_name "unknown";
    String description "Concentration C18 4";
    String long_name "C18 4";
    String units "per mil";
  }
  C18_2 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.54, 2.11;
    String bcodmo_name "unknown";
    String description "Concentration C18 2";
    String long_name "C18 2";
    String units "per mil";
  }
  C18_1 {
    Float32 _FillValue NaN;
    Float32 actual_range 17.76, 32.1;
    String bcodmo_name "unknown";
    String description "Concentration C18 1";
    String long_name "C18 1";
    String units "per mil";
  }
  C18_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 2.51, 4.04;
    String bcodmo_name "unknown";
    String description "Concentration C18 0";
    String long_name "C18 0";
    String units "per mil";
  }
  iso_C19_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 0.46;
    String bcodmo_name "unknown";
    String description "Concentration iso C19 0";
    String long_name "Iso C19 0";
    String units "per mil";
  }
  C19_1 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.12, 3.04;
    String bcodmo_name "unknown";
    String description "Concentration C19 1";
    String long_name "C19 1";
    String units "per mil";
  }
  C20_4w6 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.46, 2.36;
    String bcodmo_name "unknown";
    String description "Concentration C20 4w6";
    String long_name "C20 4W6";
    String units "per mil";
  }
  C20_5w3 {
    Float32 _FillValue NaN;
    Float32 actual_range 1.13, 5.18;
    String bcodmo_name "unknown";
    String description "Concentration C20 5w3";
    String long_name "C20 5W3";
    String units "per mil";
  }
  C20_3 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 0.27;
    String bcodmo_name "unknown";
    String description "Concentration C20 3";
    String long_name "C20 3";
    String units "per mil";
  }
  C20_2 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 0.53;
    String bcodmo_name "unknown";
    String description "Concentration C20 2";
    String long_name "C20 2";
    String units "per mil";
  }
  C20_1 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.51, 1.72;
    String bcodmo_name "unknown";
    String description "Concentration C20 1";
    String long_name "C20 1";
    String units "per mil";
  }
  C20_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.5, 1.62;
    String bcodmo_name "unknown";
    String description "Concentration C20 0";
    String long_name "C20 0";
    String units "per mil";
  }
  C22_6w6 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.08, 0.51;
    String bcodmo_name "unknown";
    String description "Concentration C22 6w6";
    String long_name "C22 6W6";
    String units "per mil";
  }
  C22_6w3 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.62, 2.7;
    String bcodmo_name "unknown";
    String description "Concentration C22 6w3";
    String long_name "C22 6W3";
    String units "per mil";
  }
  C22_5w6 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.12, 0.47;
    String bcodmo_name "unknown";
    String description "Concentration C22 5w6";
    String long_name "C22 5W6";
    String units "per mil";
  }
  C22_5w3 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.14, 0.71;
    String bcodmo_name "unknown";
    String description "Concentration C22 5w3";
    String long_name "C22 5W3";
    String units "per mil";
  }
  C22_1 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 0.39;
    String bcodmo_name "unknown";
    String description "Concentration C22 1";
    String long_name "C22 1";
    String units "per mil";
  }
  C22_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.4, 0.84;
    String bcodmo_name "unknown";
    String description "Concentration C22 0";
    String long_name "C22 0";
    String units "per mil";
  }
  C24_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.8, 1.92;
    String bcodmo_name "unknown";
    String description "Concentration C24 0";
    String long_name "C24 0";
    String units "per mil";
  }
  C25_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.12, 0.66;
    String bcodmo_name "unknown";
    String description "Concentration C25 0";
    String long_name "C25 0";
    String units "per mil";
  }
  C26_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.42, 1.25;
    String bcodmo_name "unknown";
    String description "Concentration C26 0";
    String long_name "C26 0";
    String units "per mil";
  }
  C28_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.17, 0.87;
    String bcodmo_name "unknown";
    String description "Concentration C28 0";
    String long_name "C28 0";
    String units "per mil";
  }
  C30_0 {
    Float32 _FillValue NaN;
    Float32 actual_range 0.0, 0.67;
    String bcodmo_name "unknown";
    String description "Concentration C30 0";
    String long_name "C30 0";
    String units "per mil";
  }
 }
  NC_GLOBAL {
    String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv";
    String acquisition_description 
"The three ponds are located in the high marsh (1.43-1.46 m above North
American Vertical Datum of 1988) of the Plum Island Ecosystems \\u2013 Long
Term Ecological Research (PIE-LTER) site. Surface sediments were collected
weekly for TOC, TN, \\u03b413C, and \\u03b415N (June 25-August 13 and November
11-25, 2014). Cores (5 cm diameter x 2 cm deep) were collected from three 1 m2
quadrats placed at random locations along two crisscrossing transects in each
pond. Sediments were combined in combusted glass vials to form composite
samples and stored (-80 \\u2103) until analysis. Lipid biomarker compounds were
extracted using a modified Bligh & Dyer (1959) method (Spivak and Reeve 2015).
Sediments were extracted with a methanol : chloroform : phosphate buffer
saline mixture (2:1:0.8, v:v:v) using a microwave-accelerated reaction system
(MARS6); samples were heated to 80\\u00b0C for 10 min with continuous stirring.
Samples were then partitioned and the organic phase removed. The total lipid
extract was concentrated under N2 and samples were eluted on silica gel
columns with chloroform, acetone (F1/2), and methanol (F3) (Guckert et al.
1985). The F3 (phospholipids) was dried under N2 and saponified with 0.5 M
NaOH at 70\\u00b0C for 4 h. Saponified samples were acidified and extracted 3
times with hexane. The extract was methylated with acidic methanol (95:5
methanol:HCl) and heated overnight at 70\\u00b0C to form fatty acid methyl
esters (FAME). Samples were analyzed with an Agilent 7890 gas chromatograph
with the effluent split ~70:30 between a 5975C mass spectrometer and a flame
ionization detector. Compounds were separated on an Agilent DB-5 ms column (60
m, 0.25 mm inner diameter, 0.25 \\u03bcm film). FAME concentrations were
quantified using methyl heneicosanoate as an internal standard. FAs are
designated A:B\\u03c9C, where A is the number of carbon atoms, B is the number
of double bonds, and C is the position of the first double bond from the
aliphatic \\u2018\\u03c9\\u2019 end of the molecule. Iso- and anteiso refer to
whether the methyl group of branched compounds is attached to the penultimate
or antepenultimate carbon atom.";
    String awards_0_award_nid "529582";
    String awards_0_award_number "OCE-1233678";
    String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1233678";
    String awards_0_funder_name "NSF Division of Ocean Sciences";
    String awards_0_funding_acronym "NSF OCE";
    String awards_0_funding_source_nid "355";
    String awards_0_program_manager "David L. Garrison";
    String awards_0_program_manager_nid "50534";
    String cdm_data_type "Other";
    String comment 
"Concentrations of PFLAs 
  A. Spivak, PI 
  Version June 5 2018";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_type "institution";
    String creator_url "https://www.bco-dmo.org/";
    String data_source "extract_data_as_tsv version 2.3  19 Dec 2019";
    String date_created "2018-06-05T20:13:17Z";
    String date_modified "2018-07-17T18:35:04Z";
    String defaultDataQuery "&amp;time&lt;now";
    String doi "10.1575/1912/bco-dmo.738160.1";
    String history 
"2024-11-08T05:43:04Z (local files)
2024-11-08T05:43:04Z https://erddap.bco-dmo.org/tabledap/bcodmo_dataset_738160.das";
    String infoUrl "https://www.bco-dmo.org/dataset/738160";
    String institution "BCO-DMO";
    String instruments_0_acronym "Gas Chromatograph";
    String instruments_0_dataset_instrument_description "Used to analyze samples";
    String instruments_0_dataset_instrument_nid "738354";
    String instruments_0_description "Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC)";
    String instruments_0_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB02/";
    String instruments_0_instrument_name "Gas Chromatograph";
    String instruments_0_instrument_nid "661";
    String instruments_0_supplied_name "Agilent 7890 gas chromatograph";
    String instruments_1_dataset_instrument_description "Used for sediment samples";
    String instruments_1_dataset_instrument_nid "738353";
    String instruments_1_description 
"Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.

Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/";
    String instruments_1_instrument_name "Push Corer";
    String instruments_1_instrument_nid "628287";
    String instruments_1_supplied_name "Corer";
    String keywords "1w7, 1w9c, 1w9t, 4w6, 5w3, 5w6, 6w3, 6w6, anteiso, anteiso_C13, anteiso_C15, anteiso_C17, bco, bco-dmo, biological, c12, C12_0, c13, C13_0, c14, C14_0, c15, C15_0, c16, C16_0, c17, C17_0, C17_1, c18, C18_0, C18_1, C18_2, C18_4, c19, C19_1, c20, C20_0, C20_1, C20_2, C20_3, C20_4w6, C20_5w3, c22, C22_0, C22_1, C22_5w3, C22_5w6, C22_6w3, C22_6w6, c24, C24_0, c25, C25_0, c26, C26_0, c28, C28_0, c30, C30_0, chemical, data, dataset, date, dmo, erddap, iso, iso_16_1w7, iso_16_1w9c, iso_16_1w9t, iso_C13, iso_C14, iso_C15, iso_C16, iso_C17, iso_C19_0, management, methyl, methyl_10_C16, oceanography, office, pond, preliminary, sampling, season, tide, time, week";
    String license "https://www.bco-dmo.org/dataset/738160/license";
    String metadata_source "https://www.bco-dmo.org/api/dataset/738160";
    String param_mapping "{'738160': {}}";
    String parameter_source "https://www.bco-dmo.org/mapserver/dataset/738160/parameters";
    String people_0_affiliation "Woods Hole Oceanographic Institution";
    String people_0_affiliation_acronym "WHOI";
    String people_0_person_name "Amanda Spivak";
    String people_0_person_nid "529580";
    String people_0_role "Principal Investigator";
    String people_0_role_type "originator";
    String people_1_affiliation "Woods Hole Oceanographic Institution";
    String people_1_affiliation_acronym "WHOI BCO-DMO";
    String people_1_person_name "Hannah Ake";
    String people_1_person_nid "650173";
    String people_1_role "BCO-DMO Data Manager";
    String people_1_role_type "related";
    String project "benthic_PP_at_TIDE";
    String projects_0_acronym "benthic_PP_at_TIDE";
    String projects_0_description 
"Extracted from the NSF award abstract:
This project will address how rates of benthic microalgal production respond to eutrophication and geomorphological changes in human-impacted tidal creeks. Excess nutrient loading increases benthic algal biomass and likely stimulates production rates but the magnitude of nutrient and geomorphological effects on rates of production is unknown. Will changes in benthic algal productivity affect algal-bacterial coupling? Furthermore, how is algal-bacterial coupling affected by geomorphological changes, which may be exacerbated by excess nutrient loading but can also occur in pristine marshes?
This project will take advantage of the infrastructure of the TIDE project, a long-term saltmarsh eutrophication experiment at the Plum Island Ecosystem - Long Term Ecological Research site in Northeastern Massachusetts. Specifically, the PIs will measure benthic metabolism and examine algal- bacterial coupling in fertilized and ambient nutrient tidal creeks in the first field season. The following field season, they will compare sediment metabolism and carbon dynamics on slumped tidal creek walls (i.e. areas where low marsh has collapsed into the tidal creek) to that on the bottom of tidal creeks. In both years, gross and net production will be determined using an innovative triple oxygen isotope technique and traditional dissolved oxygen and inorganic carbon flux measurements. Comparisons between these methods will be useful in informing studies of sediment metabolism. Lipid biomarkers will be used to characterize the sources of organic matter to creek sediments, and stable isotope analysis of bacterial specific biomarkers to identify the sources of organic carbon utilized by sediment bacteria. The biomarkers will reveal whether sediment bacteria use organic matter substrates, such as benthic microalgal carbon, selectively or in proportion to availability. Overall, results from the proposed study will provide important information about how sediment carbon dynamics in shallow tidal creeks respond to long term eutrophication. Furthermore, findings will enhance understanding of the role of tidal creeks in coastal biogeochemistry.";
    String projects_0_end_date "2015-08";
    String projects_0_geolocation "Plum Island Estuary, Rowley Massachusetts";
    String projects_0_name "Eutrophication Effects on Sediment Metabolism and Benthic Algal-bacterial Coupling: An Application of Novel Techniques in a LTER Estuary";
    String projects_0_project_nid "529583";
    String projects_0_start_date "2012-09";
    String publisher_name "Biological and Chemical Oceanographic Data Management Office (BCO-DMO)";
    String publisher_type "institution";
    String sourceUrl "(local files)";
    String standard_name_vocabulary "CF Standard Name Table v55";
    String summary "Concentrations of phospholipid-linked fatty acids (PLFAs) in the surface sediments of three marsh ponds in PIE-LTER (Rowley, MA). Data were collected over 11 weeks in the summer and fall of 2014.";
    String title "[Concentrations of phospholipid-linked fatty acids (PLFAs)] - Concentrations of phospholipid-linked fatty acids (PLFAs) in the surface sediments of three marsh ponds in PIE-LTER (Rowley, MA) from 2014. (Eutrophication Effects on Sediment Metabolism and Benthic Algal-bacterial Coupling: An Application of Novel Techniques in a LTER Estuary)";
    String version "1";
    String xml_source "osprey2erddap.update_xml() v1.3";
  }
}

Using tabledap to Request Data and Graphs from Tabular Datasets

tabledap lets you request a data subset, a graph, or a map from a tabular dataset (for example, buoy data), via a specially formed URL. tabledap uses the OPeNDAP (external link)

Data Access Protocol (DAP) (external link)

and its selection constraints (external link)

The URL specifies what you want: the dataset, a description of the graph or the subset of the data, and the file type for the response.

(easy) You can get data by using the dataset's Data Access Form or Subset form. They make the URL for you.
(easy) You can make a graph or map by using the dataset's Make A Graph form. It makes the URL for you.
(not hard) You can bypass the forms and get the data or make a graph or map by generating the URL by hand or with a computer program or script.

Tabledap request URLs must be in the form
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/datasetID.fileType{?query}
For example,
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/pmelTaoDySst.htmlTable?longitude,latitude,time,station,wmo_platform_code,T_25&time>=2015-05-23T12:00:00Z&time<=2015-05-31T12:00:00Z
Thus, the query is often a comma-separated list of desired variable names, followed by a collection of constraints (e.g., variable<value), each preceded by '&' (which is interpreted as "AND").

For details, see the tabledap Documentation.