http://lod.bco-dmo.org/id/dataset/739162
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-06-22
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Determining the effects of prey combination on larval Elacatinus colini standard length and survival.
2018-06-22
publication
2018-06-22
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-07
publication
https://doi.org/10.1575/1912/bco-dmo.739162.1
Peter Buston
Boston University
principalInvestigator
John Majoris
Boston University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Buston, P., Majoris, J. (2018) Determining the effects of prey combination on larval Elacatinus colini standard length and survival. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-06-22 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.739162.1 [access date]
Determining the effects of prey combination on larval Elacatinus colini standard length and survival. Dataset Description: <p>Determining the effects of prey density and combination on the standard length and survival of larval Elacatinus lori and E. colini.</p> Methods and Sampling: <p>Rotifer density experiment: To determine optimal rotifer density for newly hatched E. lori and E. colini, survival and growth of larvae were evaluated under 4 different rotifer density treatments: 0 (unfed control), 10, 15, and 20 rotifers ml-1. Twelve, 6.5-L rearing bins were set up for each species, allowing for 3 replicates per density treatment. On the day of hatch (0 dph), 25 larvae were transferred to each rearing bin. Rotifer density treatments were assigned to bins at the start of trials using a complete randomized block design. Following daily water exchange, each rearing bin was dosed with the assigned rotifer density. There was no significant difference in water quality parameters among rotifer density treatments (all Kruskal-Wallis tests, p&gt; 0.05). On day 6, all surviving larvae were collected from the rearing bins, counted and photographed using a dissection microscope. The photographs of larvae were used to compare larval size (SL) among rotifer density treatment. Artemia density experiment: To determine the optimal density of Artemia for culturing E. lori and E. colini larvae, the survival and growth of larvae were evaluated under 4 density treatments: 0 (unfed control), 3, 6, and 9 Artemia ml-1. A pilot experiment indicated that &gt;40% of E. colini larvae began consuming Artemia nauplii at 6 dph. Therefore, for each species, larvae from a single clutch were reared communally in a 38-L rearing bin and fed 15 rotifers ml-1 from 0 – 6 dph. On day 6, surviving larvae were distributed evenly among twelve, 6.5-L rearing bins (3 bins per Artemia density treatment). Due to differential survival to day 6, the number of larvae distributed among the rearing bins varied by species (E. lori: n=20 larvae bin-1; E. colini: n=14 larvae bin-1). Artemia density treatments were assigned to bins at the start of trials using a complete randomized block design. Following daily water exchange, each bin was dosed with rotifers (15 ml-1) and the assigned Artemia density. The photographs of larvae were used to compare larval size (SL) among Artemia density treatments. Plankton, Rotifers and Artemia Experiment: To determine the suitability of wild caught plankton for rearing larvae in the lab in Belize, the growth and survival of E. colini larvae fed a combination of rotifers and Artemia (RA) was compared with larvae fed solely on wild caught plankton (P). Prey combination treatments were assigned to bins at the start of trials using a complete randomized block design. On the day of hatch (0 dph), 25 larvae were transferred to each of six, 6.5-L rearing bins (3 bins per prey combination). Rotifers (15 ml-1) or plankton (≤10 ml-1) were fed to larvae beginning at 0 dph. However, Artemia (3 ml-1) were not included in the RA diet until 6 dph. Due to natural variation in the quantity of plankton collected in the field each evening, the average density of plankton fed to larvae was 5.3 ± 3.8 prey ml-1 (mean ± SD). Following daily water exchange, each rearing bin was dosed with the assigned prey combination. Water quality parameters were not significantly different between prey treatments (all Wilcoxon Rank-sum tests, p &gt; 0.05). On day 14, all remaining larvae were counted and photographed. The photographs of larvae were used to compare larval size (SL) among prey treatments.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1459546 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1459546
completed
Peter Buston
Boston University
617-358-5412
Department of Biology 5 Cummington Mall
Boston
MA
02215
USA
buston@bu.edu
pointOfContact
John Majoris
Boston University
617-358-6968
Department of Biology and Marine Program, BU 5 Cummington Mall
Boston
MA
02215
USA
jmajoris@bu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
date
sp
batch_id
treat
density
combination
bin_id
larva_id
surv
TL1
TL2
TL3
tl_avg
SL1
SL2
SL3
sl_avg
BD1
BD2
BD3
bd_avg
Custom designed swimming flume
theme
None, User defined
date
species
sample description
treatment
density
sample identification
length
featureType
BCO-DMO Standard Parameters
Swimming Flume
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: The Role of Larval Orientation Behavior in Determining Population Connectivity
https://www.bco-dmo.org/project/651265
Collaborative Research: The Role of Larval Orientation Behavior in Determining Population Connectivity
<p><em>Description from NSF award abstract:</em><br />
Understanding how far young fish move away from their parents is a major goal of marine ecology because this dispersal can make connections between distinct populations and thus influence population size and dynamics. Understanding the drivers of population dynamics is, in turn, essential for effective fisheries management. Marine ecologists have used two different approaches to understand how fish populations are connected: genetic methods that measure connectivity and oceanographic models that predict connectivity. There is, however, a mismatch between the predictions of oceanographic models and the observations of genetic methods. It is thought that this mismatch is caused by the behavior of the young, or larval, fish. The objective of this research is to study the orientation capabilities of larval fish in the wild throughout development and under a variety of environmental conditions to see if the gap between observations and predictions of population connectivity can be resolved. The project will have broader impacts in three key areas: integration of research and teaching by training young scientists at multiple levels; broadening participation of undergraduates from underrepresented groups; and wide dissemination of results through development of a website with information and resources in English and Spanish.</p>
<p>The overall objective of the research is to investigate the role of larval orientation behavior throughout ontogeny in determining population connectivity. This will be done using the neon goby, Elacatinus lori, as a model system in Belize. The choice of study system is motivated by the fact that direct genetic methods have already been used to describe the complete dispersal kernel for this species, and these observations indicate that dispersal is less extensive than predicted by a high-resolution biophysical model; E. lori can be reared in the lab from hatching to settlement providing a reliable source of larvae of all ages for proposed experiments; and a new, proven behavioral observation platform, the Drifting In Situ Chamber (DISC), allows measurements of larval orientation behavior in open water. The project has three specific objectives: to understand ontogenetic changes in larval orientation capabilities by correlating larval orientation behavior with developmental sensory anatomy; to analyze variation in the precision of larval orientation in different environmental contexts through ontogeny; and to test alternative hypotheses for the goal of larval orientation behavior, i.e., to determine where larvae are heading as they develop.</p>
Elacatinus Dispersal II
largerWorkCitation
project
eng; USA
oceans
-88.0815
-88.0815
16.815333
16.815333
2015-10-11
2015-11-30
Belizean Barrier Reef System
0
BCO-DMO catalogue of parameters from Determining the effects of prey combination on larval Elacatinus colini standard length and survival.
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/739243.rdf
Name: date
Units: unitless
Description: Date of swim trial; yyyy/mm/dd
http://lod.bco-dmo.org/id/dataset-parameter/739244.rdf
Name: sp
Units: unitless
Description: Reef fish species
http://lod.bco-dmo.org/id/dataset-parameter/739245.rdf
Name: batch_id
Units: unitless
Description: Identifies the clutch or batch of larvae
http://lod.bco-dmo.org/id/dataset-parameter/739246.rdf
Name: treat
Units: unitless
Description: Prey type treatment: Artemia; Rotifers; or a combination of plankton, Rotifers, and Artemia (PRA).
http://lod.bco-dmo.org/id/dataset-parameter/739247.rdf
Name: density
Units: prey per mililiter
Description: Prey density treatments: 0 = unfed control; 3; 6; 9
http://lod.bco-dmo.org/id/dataset-parameter/739248.rdf
Name: combination
Units: unitless
Description: Prey combination
http://lod.bco-dmo.org/id/dataset-parameter/739249.rdf
Name: bin_id
Units: unitless
Description: Rearing bin ID (1 -24)
http://lod.bco-dmo.org/id/dataset-parameter/739250.rdf
Name: larva_id
Units: unitless
Description: Larva ID
http://lod.bco-dmo.org/id/dataset-parameter/739251.rdf
Name: surv
Units: unitless
Description: Survival (1 = larva survived; 0 = larva perished)
http://lod.bco-dmo.org/id/dataset-parameter/739252.rdf
Name: TL1
Units: milimeter
Description: Total length measurements of each larva
http://lod.bco-dmo.org/id/dataset-parameter/739253.rdf
Name: TL2
Units: milimeter
Description: Total length measurements of each larva
http://lod.bco-dmo.org/id/dataset-parameter/739254.rdf
Name: TL3
Units: milimeter
Description: Total length measurements of each larva
http://lod.bco-dmo.org/id/dataset-parameter/739255.rdf
Name: tl_avg
Units: milimeter
Description: Average of the 3 total length measurements of a larva
http://lod.bco-dmo.org/id/dataset-parameter/739256.rdf
Name: SL1
Units: milimeter
Description: Standard length measurements of each larva
http://lod.bco-dmo.org/id/dataset-parameter/739257.rdf
Name: SL2
Units: milimeter
Description: Standard length measurements of each larva
http://lod.bco-dmo.org/id/dataset-parameter/739258.rdf
Name: SL3
Units: milimeter
Description: Standard length measurements of each larva
http://lod.bco-dmo.org/id/dataset-parameter/739259.rdf
Name: sl_avg
Units: milimeter
Description: Average of the 3 standard length measurements of a larva
http://lod.bco-dmo.org/id/dataset-parameter/739260.rdf
Name: BD1
Units: milimeter
Description: Body depth measurements of each larva
http://lod.bco-dmo.org/id/dataset-parameter/739261.rdf
Name: BD2
Units: milimeter
Description: Body depth measurements of each larva
http://lod.bco-dmo.org/id/dataset-parameter/739262.rdf
Name: BD3
Units: milimeter
Description: Body depth measurements of each larva
http://lod.bco-dmo.org/id/dataset-parameter/739263.rdf
Name: bd_avg
Units: milimeter
Description: Average of the 3 body depth measurements of a larva
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
118662
https://darchive.mblwhoilibrary.org/bitstream/1912/24225/1/dataset-739162_aquaculture-treatments__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.739162.1
download
onLine
dataset
<p>Rotifer density experiment: To determine optimal rotifer density for newly hatched E. lori and E. colini, survival and growth of larvae were evaluated under 4 different rotifer density treatments: 0 (unfed control), 10, 15, and 20 rotifers ml-1. Twelve, 6.5-L rearing bins were set up for each species, allowing for 3 replicates per density treatment. On the day of hatch (0 dph), 25 larvae were transferred to each rearing bin. Rotifer density treatments were assigned to bins at the start of trials using a complete randomized block design. Following daily water exchange, each rearing bin was dosed with the assigned rotifer density. There was no significant difference in water quality parameters among rotifer density treatments (all Kruskal-Wallis tests, p&gt; 0.05). On day 6, all surviving larvae were collected from the rearing bins, counted and photographed using a dissection microscope. The photographs of larvae were used to compare larval size (SL) among rotifer density treatment. Artemia density experiment: To determine the optimal density of Artemia for culturing E. lori and E. colini larvae, the survival and growth of larvae were evaluated under 4 density treatments: 0 (unfed control), 3, 6, and 9 Artemia ml-1. A pilot experiment indicated that &gt;40% of E. colini larvae began consuming Artemia nauplii at 6 dph. Therefore, for each species, larvae from a single clutch were reared communally in a 38-L rearing bin and fed 15 rotifers ml-1 from 0 – 6 dph. On day 6, surviving larvae were distributed evenly among twelve, 6.5-L rearing bins (3 bins per Artemia density treatment). Due to differential survival to day 6, the number of larvae distributed among the rearing bins varied by species (E. lori: n=20 larvae bin-1; E. colini: n=14 larvae bin-1). Artemia density treatments were assigned to bins at the start of trials using a complete randomized block design. Following daily water exchange, each bin was dosed with rotifers (15 ml-1) and the assigned Artemia density. The photographs of larvae were used to compare larval size (SL) among Artemia density treatments. Plankton, Rotifers and Artemia Experiment: To determine the suitability of wild caught plankton for rearing larvae in the lab in Belize, the growth and survival of E. colini larvae fed a combination of rotifers and Artemia (RA) was compared with larvae fed solely on wild caught plankton (P). Prey combination treatments were assigned to bins at the start of trials using a complete randomized block design. On the day of hatch (0 dph), 25 larvae were transferred to each of six, 6.5-L rearing bins (3 bins per prey combination). Rotifers (15 ml-1) or plankton (≤10 ml-1) were fed to larvae beginning at 0 dph. However, Artemia (3 ml-1) were not included in the RA diet until 6 dph. Due to natural variation in the quantity of plankton collected in the field each evening, the average density of plankton fed to larvae was 5.3 ± 3.8 prey ml-1 (mean ± SD). Following daily water exchange, each rearing bin was dosed with the assigned prey combination. Water quality parameters were not significantly different between prey treatments (all Wilcoxon Rank-sum tests, p &gt; 0.05). On day 14, all remaining larvae were counted and photographed. The photographs of larvae were used to compare larval size (SL) among prey treatments.</p>
Specified by the Principal Investigator(s)
<p>R version 3.2.3</p>
<p><strong>BCO-DMO Data Processing Notes:</strong></p>
<p>-replaced "." with nd -replaced species codes in sp column with full species name -reformatted column names to comply with BCO-DMO standards -reformatted date to yyyy/mm/dd -combined rotifer, artemia, and PRA files</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Custom designed swimming flume
Custom designed swimming flume
PI Supplied Instrument Name: Custom designed swimming flume PI Supplied Instrument Description:Used to analyze fish swimming behavior Instrument Name: Swimming Flume Instrument Short Name: Instrument Description: A tool used to analyze and quantify fish swimming behavior, physiology, and performance.