http://lod.bco-dmo.org/id/dataset/741192
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-07-24
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Lipid and glycogen content of A. pisonii eggs from three habitats along the Florida eastern coast, 2016
2018-07-16
publication
2018-07-16
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-07-16
publication
https://doi.org/10.26008/1912/bco-dmo.741192.1
Dr Blaine D. Griffen
University of South Carolina
principalInvestigator
Zachary J. Cannizzo
University of South Carolina
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Griffen, B., Cannizzo, Z. (2020) Lipid and glycogen content of A. pisonii eggs from three habitats along the Florida eastern coast, 2016. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-07-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.741192.1 [access date]
Egg lipid and glycogen content of A. pisonii from three habitats Dataset Description: <p>This dataset includes calculations of the lipid and glycogen content of&nbsp;eggs of mangrove tree crabs, A. pisonii, from three habitats: mangrove, saltmarsh and mangrove collected along the eastern Florida coast in 2016.</p> Methods and Sampling: <p>We collected 10 ovigerous females with stage-1 non-eyed eggs by hand and from each habitat (mangrove, salt marsh, dock) during the week preceding the full moon of each of five consecutive months throughout the <em>A. pisonii </em>reproductive season. Crabs were immediately placed on dry ice and stored at -80<sup>o</sup>C until dissection at which time the size and gut-width were determined and the whole egg clutch was carefully removed from the pleopods. The egg clutch was then freeze-fried and stored at -80<sup>o</sup>C until analysis.</p>
<p>We used a modified Folch Extraction to extract the lipids from a subset (~20-40mg) of each clutch.</p>
<p>We used a subset (~10mg) of each clutch to compare the glycogen content of eggs between habitats. We did so using a Sigma-Aldrich Glycogen Assay Kit MAK016 and determined the glycogen concentration as a percentage of egg mass through colorimetry per the manufacturer instructions.</p>
<p>Mass of extracted lipids was calculated by subtracting the mass of the vial in which lipids was extracted from the combined mass of the lipids and the vial.</p>
<p>Glycogen concentration was calculated using colorimetry per the manufacturer instructions.</p>
<p>Locations, Florida East Coast:<br />
Round Island Park: 27o33'33"N 80o19'53"W<br />
Pepper Park: 27o29'42'N 80o18'12"W<br />
North Causeway Park: 27o28'28"N 80o19'12"W<br />
Oslo Road: 27o35'14"N 80o21'55"W<br />
Anastasia State Park: 29o52'40"N 81o16'32"W<br />
Guana-Tolomato-Matanzas NERR: 30o0'49"N 81o20'42"W<br />
Vilano Inlet: 29o55'16"N 81o17'57"W<br />
Palm Valley/Nocatee Canoe Launch: 30o07'57"N 81o23'08"W<br />
St. Augustine Yacht Club: 29o53'09"N 81o17'08"W<br />
Boating Club Road: 29o56'34"N 81o18'31"W</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1129166 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1129166
completed
Dr Blaine D. Griffen
University of South Carolina
801-422-5930
Provo
UT
84602
USA
blaine_griffen@byu.edu
pointOfContact
Zachary J. Cannizzo
University of South Carolina
815-353-1457
2524 Kiawah Ave.
Columbia
SC
29205
zac.cannizzo@gmail.com
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Habitat
Site
ISO_Date
ID
CW
GW
GW_CW
Egg_W
F_Vial
Vial_Lipid
Lipid
Lipid_wt
Lipid_pct
Glycogen_W
Glycogen_Conc
Glycogen_pct
Parr semimicro bomb calorimeter
theme
None, User defined
site description
site
date
individual
width
No BCO-DMO term
weight
lipids in biota
featureType
BCO-DMO Standard Parameters
scale
bomb calorimeter
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Linking Variation in Metabolic Processes as a Key to Prediction
https://www.bco-dmo.org/project/562104
Linking Variation in Metabolic Processes as a Key to Prediction
<p><em>Description from NSF award abstract:</em><br />
A major goal of biological and ecological sciences is to understand natural systems well enough to predict how species and populations will respond to a rapidly changing world (i.e., climate change, habitat loss, etc.). A population under any conditions will grow, shrink, or disappear altogether depending on how efficiently individuals consume resources (food), utilize that food metabolically, and eventually reproduce. However, making accurate predictions based on these metabolic processes is complicated by the realities that each species has different resource requirements and that no two individuals within a species are exactly alike. Rather, individuals vary and this variation, both within and across species, is central to many ecological and evolutionary processes. Developing the ability to predict responses of biological systems to a changing world therefore requires a mechanistic understanding of variation. The goal of this project is to improve this mechanistic understanding by examining variation within a metabolic context across a range of species that have a spectrum of commonly-seen resource requirements. Further, the work capitalizes on a unique biological characteristic of this group of species that allows control and manipulation of individual reproduction, facilitating experimental study of the mechanistic links between variation in individual consumption, metabolism, and reproduction. The foundation this research is a combination of field measurements and laboratory experiments using both well-established and newly-developed techniques to quantify these links. The result will be a quantitative framework to predict how individuals will respond reproductively to changes in resource use. Because of the close link between individual reproduction and population dynamics, this research will contribute substantially to predictions in population dynamics under realistic conditions where individuals use more than a single resource, and improve the prediction of responses to current and future ecological changes.</p>
<p><strong>The following publications and data resulted from this project:</strong></p>
<p>Belgrad, B. and B. Griffen. 2016. Predator-prey interactions mediated by prey personality and predator identity.<em>Proc. Roy. Soc. B</em>: In Review. [2016-01-20]<br /><a href="http://www.bco-dmo.org/dataset/635735">P. herbstii mortality data</a>: Mortality of crabs when exposed to either a single blue crab, toadfish, or no predator for a week<br /><a href="http://www.bco-dmo.org/dataset/635805">P. herbstii personality data</a>: Refuge use of crabs when exposed to predator odor cues from either blue crabs, toadfish, or control of no cue<br /><a href="http://www.bco-dmo.org/dataset/635833">P. herbstii predator behavior data</a>: Refuge use and mobility of blue crabs and toadfish while in mesocosms for a week - behavior measured during two days.</p>
<p>Belgrad, B. and B. Griffen. 2016. The influence of dietary shifts on fitness of the blue crab, <em>Callinectes sapidus</em>. <em>PloS One. DOI: <a href="http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145481" target="_blank">10.1371/journal.pone.0145481</a>.</em><br /><a href="http://www.bco-dmo.org/dataset/630014">Blue crab activity</a>: Activity of crabs fed different diets over a summer<br /><a href="http://www.bco-dmo.org/dataset/629934">Blue crab egg size</a>: Volume of eggs for crabs fed different diets<br /><a href="http://www.bco-dmo.org/dataset/629856">Blue crab hepatopancreas index (HSI)</a>: Weight of hepatopancreas for crabs fed different diets<br /><a href="http://www.bco-dmo.org/dataset/629878">Blue crab hepatopancreas lipid content</a>: Hepatopancreas lipid content of crabs fed different diets<br /><a href="http://www.bco-dmo.org/dataset/629830">Blue crab reproductive tissue analysis (GSI)</a>: Gonadosomatic index of blue crabs on various diets<br /><a href="http://www.bco-dmo.org/dataset/629965">Blue crab survival</a>: Blue crab survival data during the dietary study</p>
<p>Knotts ER, Griffen BD. 2016. Individual movement rates are sufficient to determine and maintain dynamic spatial positioning within <em>Uca pugilator</em> herds. <em>Behavioral Ecology and Sociobiology</em> 70:639-646<br /><a href="http://www.bco-dmo.org/dataset/642465" target="_blank">Uca pugilator: behavior change with carapace marking</a>: Search space behavior due to carapace treatment (control, nail polish, and food dye)<br /><a href="http://www.bco-dmo.org/dataset/642057" target="_blank">Uca pugilator: field spatial position</a>: Assessment of individual's position within a herd at 3 min. intervals; for proportion of time found at edge of herd<br /><a href="http://www.bco-dmo.org/dataset/642081" target="_blank">Uca pugilator: herd position proportion</a>: Individual's proportion of time spent in an edge/alone position among a herd<br /><a href="http://www.bco-dmo.org/dataset/642447" target="_blank">Uca pugilator: search space distribution</a>: Search space that crabs traveled; to evaluate the sample's distribution of exploratory behavior</p>
<p>Belgrad, B. and B. Griffen. 2015. Rhizocephalan infection modifies host food consumption by reducing host activity levels. <em>Journal of Experimental Marine Biology and Ecology</em>. 466: 70-75.<br /><a href="http://www.bco-dmo.org/dataset/638671">E. depressus digestion time</a> : Time taken for food to pass through gut of flat-backed mud crabs infected by a parasite<br /><a href="http://www.bco-dmo.org/dataset/638697">E. depressus metabolism</a>: Respiration rate of infected/uninfected flat-backed mud crabs<br /><a href="http://www.bco-dmo.org/dataset/638726">E. depressus reaction time to prey</a>: Time taken for infected/uninfected flat-backed mud crabs to react to the presence of prey</p>
<p>Blakeslee, A.M., C.L. Keogh, A.E. Fowler, B. Griffen. 2015. Assessing the effects of trematode infection on invasive green crabs in eastern North America. <em>PLOS One</em> 10(6): e0128674.(<a href="http://dmoserv3.bco-dmo.org/data_docs/Variation_Metabolic_Processes/Blakeslee_etal_PlosOne_2015.pdf" target="_blank">pdf</a>)<br /><a href="http://www.bco-dmo.org/dataset/642488" target="_blank">Carcinus: hemocyte density</a>: Counts of circulating hemocyte density in Carcinus maenas<br /><a href="http://www.bco-dmo.org/dataset/642804" target="_blank">Carcinus: parasites physiology behavior</a>: Behavior and physiology of Carcinus maenas infected with trematode parasite</p>
<p>Griffen BD, Norelli AP (2015) Spatially variable habitat quality contributes to within-population variation in reproductive success. <em>Ecology and Evolution</em> 5:1474-1483.<br /><a href="http://www.bco-dmo.org/dataset/648291">P. herbstii diet: sampling site characteristics (Eco-Evo 2015)</a><br /><a href="http://www.bco-dmo.org/dataset/648300">P. herbstii diet: body measurements (Eco-Evo 2015)</a><br /><a href="http://www.bco-dmo.org/dataset/648281">P. herbstii diet & reproduction (Eco-Evo 2015)<br />
P. herbstii: collection sites (Ecol-Evol 2015)</a></p>
<p>Griffen BD, Riley ME (2015) Potential impacts of invasive crabs on one life history strategy of native rock crabs in the Gulf of Maine. Biological Invasions 17:2533-2544.<br /><a href="http://www.bco-dmo.org/dataset/644899">Cancer consumption and reproduction (Bio.Inv. 2015)</a>: Lab experiment linking dietary consumption and reproduction</p>
<p>Griffen BD, Vogel M, Goulding L, Hartman R (2015) Energetic effects of diet choice by invasive Asian shore crabs: implications for persistence when prey are scarce. <em>Marine Ecology Progress Series</em> 522:181-192.<br /><a href="http://www.bco-dmo.org/dataset/648239">Hemigrapsus diet 1 (MEPS 2015)</a><br /><a href="http://www.bco-dmo.org/dataset/648272">Hemigrapsus diet 2 (MEPS 2015)</a></p>
<p>Hogan and Griffen (2014). The Dietary And Reproductive Consequences Of Fishery-Related Claw Removal For The Stone Crab <em>Menippe</em> Spp. Journal of Shellfish Research, Vol. 33, No. 3, 795–804.<br /><a href="http://www.bco-dmo.org/dataset/638569">Stone crab: 052012-DietChoiceExp1</a>: Prey choice for 2-clawed and 1-clawed Stone Crabs (Menippe spp.)<br /><a href="http://www.bco-dmo.org/dataset/638622">Stone crab: 052012-LongTermConsumption</a>: Long-term consuption for 2-clawed and 1-clawed Stone Crabs (Menippe spp.), summer of 2012<br /><a href="http://www.bco-dmo.org/dataset/638602">Stone crab: 062013-DietChoiceExp2</a>: Prey choice for 2-clawed and 1-clawed Stone Crabs (Menippe spp.)<br /><a href="http://www.bco-dmo.org/dataset/638645">Stone crab: 062013-PreySizeSelection</a>: Prey Size selection ranking for 2-clawed and 1-clawed Stone Crabs (Menippe spp.)</p>
<p>Riley M, Johnston CA, Feller IC, and Griffen B. 2014. Range expansion of <em>Aratus pisonii </em>(mangrove tree crab) into novel vegetative habitats. <em>Southeastern Naturalist </em>13(4): 43-38<br /><a href="http://www.bco-dmo.org/dataset/639205">A. pisonii: range expansion</a>: Aratus pisonii survey in native mangrove and novel salt marsh habitats</p>
<p>Riley M, Vogel M, Griffen B. 2014. Fitness-associated consequences of an omnivorous diet for the mangrove tree crab <em>Aratus pisonii</em>. <em>Aquatic Biology </em>20:35-43, DOI: 10.3354/ab00543<br /><a href="http://www.bco-dmo.org/dataset/639095">A. pisonii: fitness and diet</a>: Impact of diet variation on physiological and reproductive condition of A. pisonii</p>
<p>Toscano BJ, Newsome B, Griffen BD (2014) Parasite modification of predator functional response. Oecologia 175:345-352b<br /><a href="http://www.bco-dmo.org/dataset/638907">E. depressus - parasite and feeding (Oecologia, 2014)</a>: Feeding with and without parasitic barnacle infection<br /><a href="http://www.bco-dmo.org/dataset/638930">E. depressus - parasite and prey handling (Oecologia, 2014)</a>: Food handling with and without parasitic barnacle infection<br /><a href="http://www.bco-dmo.org/dataset/638956">E. depressus - parasite study - field survey (Oecologia, 2014)</a>: Parasitised field survey</p>
<p>Toscano BJ, Griffen BD (2014) Trait-mediated functional responses: predator behavioural type mediates prey consumption.<em>Journal of Animal Ecology</em> 83:1469-1477<br /><a href="http://www.bco-dmo.org/dataset/638884">P. herbstii - activity and feeding (JAE, 2014)</a>: Activity level and feeding with and without predator cue</p>
<p>Toscano BJ, Gatto J, Griffen BD (2014) Effects of predation threat on repeatability of individual crab behavior revealed by mark recapture. <em>Behavioral Ecology and Sociobiology</em> 68:519-527<br /><a href="http://www.bco-dmo.org/dataset/638853">P. herbstii - recapture behavior (BESB, 2014)</a>: Mud crabs refuge use and activity level - initial measurements<br /><a href="http://www.bco-dmo.org/dataset/638825">P. herbstii - refuge use (BESB, 2014)</a>: Effect of predation threat on repeatability of individual crab behavior revealed by mark-recapture</p>
<p>Griffen BD, Altman I, Bess BM, Hurley J, Penfield A (2012) The role of foraging in the success of invasive species. Biological Invasions. 14:2545-2558<br /><a href="http://www.bco-dmo.org/dataset/644932">Hemigrapsus seasonal diet (Bio.Inv. 2012)</a>: Percent herbivory and gut fullness for Hemigrapsus sanguineus at different times of year</p>
<p>Griffen BD, Toscano B, Gatto J (2012) The role of intraspecific trait variation in mediating indirect interactions. Ecology 93:1935-1943<br /><a href="http://www.bco-dmo.org/dataset/644846">P. herbstii refuge use (Ecology, 2012)</a>: Proportion of time that Panopeus herbstii spent using refuge habitats in a lab experiment<br /><a href="http://www.bco-dmo.org/dataset/644883">P. herbstii: Field personality distribution (Ecology, 2012)</a>: Field distribution of personality types in the mud crab Panopeus herbstii relative to tidal height<br /><a href="http://www.bco-dmo.org/dataset/644867">P. herbstii: Trait mediated indirect effect (Ecology, 2012)</a>: Influence of refuge use by the mud crab Panopeus herbstii on consumption of bivalves</p>
<p>Riley ME, Griffen BD (2017) Habitat-specific differences alter traditional biogeographic patterns of life history in a climate-change induced range expansion. PLOS One 12(5):e0176263<br /><a href="http://www.bco-dmo.org/dataset/639282">A. pisonii: egg size</a>: Comparing egg size in Aratus pisonii populations from mangrove and salt marsh habitats<br /><a href="http://www.bco-dmo.org/dataset/639229">A. pisonii: fecundity</a>: Determining fecundity of Aratus pisonii populations in mangrove and salt marsh habitats<br /><a href="http://www.bco-dmo.org/dataset/639320">A. pisonii: larval starvation resistance</a>: Comparing larval quality in Aratus pisonii populations from mangrove and salt marsh habitats<br /><a href="http://www.bco-dmo.org/dataset/639250">A. pisonii: latitudinal body size</a>: Survey examining latitudinal body size patterns in Aratus pisonii<br /><a href="http://www.bco-dmo.org/dataset/639301">A. pisonii: predation</a>: Comparing predation pressure on Aratus pisonii in mangrove and salt marsh habitats<br /><a href="http://www.bco-dmo.org/dataset/639267">A. pisonii: reproductive effort</a>: Survey comparing Aratus pisonii reproductive effort in native and novel habitats<br /><a href="http://www.bco-dmo.org/dataset/639335">A. pisonii: herbivory</a>: Relationship between leaf herbivory, tree characteristics, and refuge availability<br /><a href="http://www.bco-dmo.org/dataset/639360">A. pisonii: mangrove tree survey</a>: Mangrove tree distribution and characteristics in a dwarf mangrove system</p>
<p>Cannizzo ZJ, Dixon SR & Griffen BD (2018). An anthropogenic habitat within a suboptimal colonized ecosystem provides improved conditions for a range-shifting species. Ecology and Evolution, 8(3):1524-1533.<br /><a href="https://www.bco-dmo.org/dataset/740014">A. pisonii: behavior</a>: Proportion of time the mangrove tree crab Aratus pisonii spent in different behaviors related to diet and energy storage<br /><a href="https://www.bco-dmo.org/dataset/740137">A. pisonii: dock-marsh thermal</a>: Thermal readings from under a dock and in a nearby salt marsh<br /><a href="https://www.bco-dmo.org/dataset/741059">A. pisonii: sun-shade</a>: Proportion of time that mangrove tree crab Aratus pisonii spent in sun and shade in three habitats, 2015-2016.<br /><a href="https://www.bco-dmo.org/dataset/741032">A. pisonii: thermal picture</a>: Thermal condition of A. pisonii in three habitats: under dock, mangroves, saltmarsh</p>
Variation in Metabolic Processes
largerWorkCitation
project
eng; USA
biota
oceans
-81.38556
-80.28611
27.43
30.1325
2016-06-14
2016-10-13
0
BCO-DMO catalogue of parameters from Lipid and glycogen content of A. pisonii eggs from three habitats along the Florida eastern coast, 2016
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/741202.rdf
Name: Habitat
Units: unitless
Description: The habitat where the crab was collected and observed.
http://lod.bco-dmo.org/id/dataset-parameter/741203.rdf
Name: Site
Units: unitless
Description: Site of observation/collection. RI= Round Island Park; PP=Pepper Park; NC=North Causeway Park; Oslo=Oslo Road; ANA=Anastasia State Park; GTM= Guana-Tolomato-Matanzas NERR; VM=Vilano Inlet; PV= Palm Valley/Nocatee Canoe Launch; YC=St. Augustine Yacht Club; BC=Boating Club Road
http://lod.bco-dmo.org/id/dataset-parameter/741204.rdf
Name: ISO_Date
Units: unitless
Description: Date in ISO format (yyyy-mm-dd)
http://lod.bco-dmo.org/id/dataset-parameter/741205.rdf
Name: ID
Units: unitless
Description: individual ID number given to each crab
http://lod.bco-dmo.org/id/dataset-parameter/741206.rdf
Name: CW
Units: millimeters (mm)
Description: Size of crab; measured as carapace-width
http://lod.bco-dmo.org/id/dataset-parameter/741207.rdf
Name: GW
Units: millimeters (mm)
Description: Gut width measured as the width of cardiac stomach.
http://lod.bco-dmo.org/id/dataset-parameter/741208.rdf
Name: GW_CW
Units: unitless
Description: gut-width:carapace-width ratio
http://lod.bco-dmo.org/id/dataset-parameter/741209.rdf
Name: Egg_W
Units: grams
Description: Dry mass of eggs from which lipid were extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741210.rdf
Name: F_Vial
Units: grams
Description: Mass of final vial in-which extracted lipids was weighed.
http://lod.bco-dmo.org/id/dataset-parameter/741211.rdf
Name: Vial_Lipid
Units: grams
Description: Mass of extracted lipids in vial.
http://lod.bco-dmo.org/id/dataset-parameter/741212.rdf
Name: Lipid
Units: grams
Description: Mass of extracted lipids.
http://lod.bco-dmo.org/id/dataset-parameter/741213.rdf
Name: Lipid_wt
Units: unitless
Description: Proportion of lipid mass to mass of eggs from which the lipids were extracted
http://lod.bco-dmo.org/id/dataset-parameter/741214.rdf
Name: Lipid_pct
Units: unitless
Description: Concentration of egg lipids in mass-percent of eggs from which lipids were extracted
http://lod.bco-dmo.org/id/dataset-parameter/741215.rdf
Name: Glycogen_W
Units: milligrams
Description: Mass of eggs from which glycogen was extracted.
http://lod.bco-dmo.org/id/dataset-parameter/741216.rdf
Name: Glycogen_Conc
Units: unitless
Description: Proportion of glycogen mass to mass of eggs from which the glycogen was extracted
http://lod.bco-dmo.org/id/dataset-parameter/741217.rdf
Name: Glycogen_pct
Units: unitless
Description: Concentration of egg glycogen in mass-percent of eggs from which glycogen was extracted
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
16590
https://darchive.mblwhoilibrary.org/bitstream/1912/25972/1/dataset-741192_pisonii-egg-lipid-and-glycogen__v1.tsv
download
https://doi.org/10.26008/1912/bco-dmo.741192.1
download
onLine
dataset
<p>We collected 10 ovigerous females with stage-1 non-eyed eggs by hand and from each habitat (mangrove, salt marsh, dock) during the week preceding the full moon of each of five consecutive months throughout the <em>A. pisonii </em>reproductive season. Crabs were immediately placed on dry ice and stored at -80<sup>o</sup>C until dissection at which time the size and gut-width were determined and the whole egg clutch was carefully removed from the pleopods. The egg clutch was then freeze-fried and stored at -80<sup>o</sup>C until analysis.</p>
<p>We used a modified Folch Extraction to extract the lipids from a subset (~20-40mg) of each clutch.</p>
<p>We used a subset (~10mg) of each clutch to compare the glycogen content of eggs between habitats. We did so using a Sigma-Aldrich Glycogen Assay Kit MAK016 and determined the glycogen concentration as a percentage of egg mass through colorimetry per the manufacturer instructions.</p>
<p>Mass of extracted lipids was calculated by subtracting the mass of the vial in which lipids was extracted from the combined mass of the lipids and the vial.</p>
<p>Glycogen concentration was calculated using colorimetry per the manufacturer instructions.</p>
<p>Locations, Florida East Coast:<br />
Round Island Park: 27o33'33"N 80o19'53"W<br />
Pepper Park: 27o29'42'N 80o18'12"W<br />
North Causeway Park: 27o28'28"N 80o19'12"W<br />
Oslo Road: 27o35'14"N 80o21'55"W<br />
Anastasia State Park: 29o52'40"N 81o16'32"W<br />
Guana-Tolomato-Matanzas NERR: 30o0'49"N 81o20'42"W<br />
Vilano Inlet: 29o55'16"N 81o17'57"W<br />
Palm Valley/Nocatee Canoe Launch: 30o07'57"N 81o23'08"W<br />
St. Augustine Yacht Club: 29o53'09"N 81o17'08"W<br />
Boating Club Road: 29o56'34"N 81o18'31"W</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
- added ISO_Date columns; removed Month (number) and Date (d-Mon) column from display<br />
-&nbsp;reduced decimal precision of: GW_CW from 9 to 3 places; Lipid_wt, Glycogen_Conc, Glycogen_pct (9 to 5)<br />
- changed Glycogen_perc to Glycogen_pct to match Lipid_pct</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Used to measure wet and dry weights of eggs Instrument Name: scale Instrument Short Name:scale Instrument Description: An instrument used to measure weight or mass. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB13/
Parr semimicro bomb calorimeter
Parr semimicro bomb calorimeter
PI Supplied Instrument Name: Parr semimicro bomb calorimeter PI Supplied Instrument Description:Used to determine the energy content of the eggs. Instrument Name: bomb calorimeter Instrument Short Name: Instrument Description: A device for determining heats of combustion by igniting a sample in a high pressure of oxygen in a sealed vessel and measuring the resulting rise in temperature.