http://lod.bco-dmo.org/id/dataset/750823
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2018-12-04
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia
2018-12-06
publication
2018-12-06
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-03-15
publication
https://doi.org/10.1575/1912/bco-dmo.750823.1
Matthew D. Johnson
Woods Hole Oceanographic Institution
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Johnson, M. (2018) Data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2018-12-06 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.750823.1 [access date]
Data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia Dataset Description: <p>This dataset contains data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia.</p> Methods and Sampling: <p>Prorocentrum minimum culturing: All cultures were maintained routinely in F/2-Si in 32 PSU seawater, at 18C and 14:10 light:dark cycle at 50 uE (u = micro). All cultures were transferred once every two weeks.</p>
<p>At each time point, 2 ml of cells were removed from experimental culture flasks and preserved with gluteraldehyde (1% final concentration) and stored at 4C until used to make microscopy slides. To make slides, 1 ml of preserved sample was filtered onto a black 2 um nucleopore polycarbonate filter, and then mounted on a glass microscope slide with fluorescence grade immersion oil. Slides were then counted using fluorescence microscopy&nbsp;and stored at -20C.</p>
<p>Culturing and experimental methods can be found in Johnson 2015.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1436169 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1436169
completed
Matthew D. Johnson
Woods Hole Oceanographic Institution
508-289-2584
266 Woods Hole Rd. Watson Building 217, MS #52
Woods Hole
MA
02543
USA
mattjohnson@whoi.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Prey
Strain
treatment
rep
cells
cells_feeding
pcnt_cells_feeding
GFI
GFI_per_cell
OFI
OFI_per_cell
theme
None, User defined
treatment
sample identification
replicate
count
featureType
BCO-DMO Standard Parameters
Fluorescence Microscope
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Exploring the physiological and ecological basis of mixotrophy in marine food webs
https://www.bco-dmo.org/project/615830
Exploring the physiological and ecological basis of mixotrophy in marine food webs
<p>Marine phytoplankton are responsible for about half of global primary production despite being seasonally or chronically nutrient limited. To cope with this, many phytoplankton supplement their nutritional needs through mixotrophy, which involves feeding on bacteria or other algae. These microscopic Venus Fly Traps of the ocean are major players in marine microbial food webs, yet we know so little about when they feed and how their eating is balanced with photosynthesis. This research will shed light on how environmental and cellular factors control mixotrophy, and how mixotrophy and photosynthesis are integrated in the overall metabolism. While understanding the ecological role of mixotrophy in ocean food webs is center to this work, results from this study will also shed light on the evolution of mixotrophy by identifying potential tradeoffs between feeding and photosynthesis.</p>
<p>Mixotrophy refers to species that combine some level of phagotrophy and phototrophy, and represents a diverse array of ecological interactions and cellular and metabolic adaptations. While often perceived as an exception to the norm, mixotrophy is commonplace in marine food webs, affording phytoplankton greater ecological fitness during periods of low or limiting nutrients while stabilizing food webs. Many mixotrophs have a low chlorophyll: carbon ratio, which tends to make them poor phototrophic competitors. In turn, feeding allows these species to achieve maximum growth while in some cases also eliminating their competitors. Other mixotrophs are strong phototrophic competitors, and only feed when severely nutrient limited. This research will determine the cellular and environmental factors that lead to feeding by marine phytoplankton, and how the contrasting metabolisms of heterotrophy and photosynthesis are integrated within a cell. This research will involve laboratory-based experiments on model dinoflagellate and chrysophyte cultures. Using microscopy, physiology, proteomics and metabolomics approaches, this work will test hypotheses about the ultimate causes and consequences of mixotrophy. The major objectives are to determine 1) environmental controls for inducing mixotrophy, 2) the role of prey quality on predator selection, 3) cellular and molecular controls of mixotrophy, and 4) nutrient assimilation and integrated metabolism. Using these various research approaches, this work will produce a comprehensive view of several mixotrophs and provide new insights into cellular, ecological, and evolutionary aspects of mixotrophy. Results from this research will improve our understanding of the physiological and ecological role of mixotrophy in marine phytoplankton, and provide much needed molecular markers for studying this process in both the laboratory and field.</p>
Mixo Foodwebs
largerWorkCitation
project
eng; USA
biota
oceans
2016-10-01
2016-10-31
laboratory: Woods Hole, Mass. USA
0
BCO-DMO catalogue of parameters from Data from an experiment that measured the occurrence of feeding among 8 Prorocentrum minimum strains on fluorescently labeled bacteria or the cryptophyte Teleaulax amphioxeia
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/750830.rdf
Name: Prey
Units: unitless
Description: Either fluorescently labeled bacteria (FLB) or the cryptophyte Teleaulax amphioxeia (CRYPT) was used
http://lod.bco-dmo.org/id/dataset-parameter/750831.rdf
Name: Strain
Units: unitless
Description: Prorocentrum minimum culture strain name
http://lod.bco-dmo.org/id/dataset-parameter/750832.rdf
Name: treatment
Units: unitless
Description: Treatments are as follows: LOG1 is 3 days growing in F/2 in log phase; LOG2 is 7 days growing in F/2 in log phase; STAT1 is 14 days growing in F/2 in stationary phase; STAT2 is 21 days growing in F/2 in stationary phase; P1 is 3 days of growing under phosphorous starvation (F/2-P); P2 is 7 days of growing under phosphorous starvation (F/2-P); P3 is 14 days of growing under phosphorous starvation (F/2-P); P4 is 21 days of growing under phosphorous starvation (F/2-P)
http://lod.bco-dmo.org/id/dataset-parameter/750833.rdf
Name: rep
Units: unitless
Description: Replicate for each strain (treatment) n=3
http://lod.bco-dmo.org/id/dataset-parameter/750834.rdf
Name: cells
Units: unitless
Description: Number of P. minimum cells counted
http://lod.bco-dmo.org/id/dataset-parameter/750835.rdf
Name: cells_feeding
Units: unitless
Description: Number of P. minimum cells with ingested fluorescently labeled prey
http://lod.bco-dmo.org/id/dataset-parameter/750836.rdf
Name: pcnt_cells_feeding
Units: unitless
Description: The percentage of cells feeding
http://lod.bco-dmo.org/id/dataset-parameter/750837.rdf
Name: GFI
Units: unitless
Description: Number of green fluorescent inclusions (GFIs); GFIs are food vacuoles of ingested FLB
http://lod.bco-dmo.org/id/dataset-parameter/750838.rdf
Name: GFI_per_cell
Units: unitless
Description: Number of GFIs per P. minimum cell
http://lod.bco-dmo.org/id/dataset-parameter/750839.rdf
Name: OFI
Units: unitless
Description: Number of orange fluorescent inclusions (OFIs); OFIs are food vacuoles from ingesting phycoerythrin-containing cryptophyte prey
http://lod.bco-dmo.org/id/dataset-parameter/750840.rdf
Name: OFI_per_cell
Units: unitless
Description: Number of OFIs per P. minimum cell
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
11376
https://darchive.mblwhoilibrary.org/bitstream/1912/23811/1/dataset-750823_pmin-strain-feeding-2__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.750823.1
download
onLine
dataset
<p>Prorocentrum minimum culturing: All cultures were maintained routinely in F/2-Si in 32 PSU seawater, at 18C and 14:10 light:dark cycle at 50 uE (u = micro). All cultures were transferred once every two weeks.</p>
<p>At each time point, 2 ml of cells were removed from experimental culture flasks and preserved with gluteraldehyde (1% final concentration) and stored at 4C until used to make microscopy slides. To make slides, 1 ml of preserved sample was filtered onto a black 2 um nucleopore polycarbonate filter, and then mounted on a glass microscope slide with fluorescence grade immersion oil. Slides were then counted using fluorescence microscopy&nbsp;and stored at -20C.</p>
<p>Culturing and experimental methods can be found in Johnson 2015.</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing:<br />
-&nbsp;modified parameter names;<br />
- filled empty cells with "nd" (no data);<br />
- combined data from two spreadsheets into one.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/