http://lod.bco-dmo.org/id/dataset/763873
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-03-28
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Sample log for HADESK bacterial biomarker phospholipid fatty acid content from sediment cores collected on R/V Thomas G. Thompson cruise TN309, May 2014
2019-03-28
publication
2019-03-28
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-17
publication
https://doi.org/10.1575/1912/bco-dmo.763873.1
Timothy M. Shank
Woods Hole Oceanographic Institution
principalInvestigator
Jeffrey C. Drazen
University of Hawaii at Manoa
principalInvestigator
Paul Yancey
Whitman College
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Shank, T., Drazen, J., Yancey, P. (2019) Sample log for HADESK bacterial biomarker phospholipid fatty acid content from sediment cores collected on R/V Thomas G. Thompson cruise TN309, May 2014. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-03-28 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.763873.1 [access date]
HADES-K bacterial biomarker phospholipid fatty acid (PLFA) Dataset Description: <p>This dataset contains the sample log for HADESK bacterial biomarker phospholipid fatty acid (PLFA) content from sediment push cores. The same samples were also used for the organic matter (OM) analysis.&nbsp; Samples were taken in the Kermadec Trench in the Southwest Pacific, 4000 to ~10,000m from the RV/ Thomas G. Thompson during cruise TN309, May 2014.</p> Methods and Sampling: <p>Push cores (6.35 cm diameter) of sediment were collected in situ using the manipulator arm of the Hybrid Remotely Operated Vehicle Nereus on the margin of the Kermadec Trench (6013m) and along the main trench axis (7137 to 9177m) at roughly 1000m increments.</p>
<p>Cores were sectioned at 1cm intervals and sieved for the meiofaunal fraction.</p>
<p>Upon retrieval of sediment cores, the 0-1, 1-2, 2-3, 3-5, 5-10 cm depth horizons were sectioned and stored frozen (-80 °C) until analysis. Benthic bacterial biomass were calculated from sediment concentrations of the bacterial biomarker phospholipid fatty acids (PLFAs) i14:0, i15:0, ai15:0, i16:0. In brief, PLFAs were extracted and purified from known quantities of freeze-dried sediment samples and subsequently derivatized to yield fatty acid methyl esters (FAMEs). FAMEs were quantified using GC-FID (Agilent Technologies 6890N). Bacterial biomass was calculated using the well-established PLFA:C conversion factor of 0.056 gC PLFA/gC biomass.</p>
<p>Attempts were made to obtain 3 replicate cores at each depth horizon (every 1000 meters from 6000m to 10,000m.&nbsp; For logistical reasons with vehicle performance (and loss at 10,000), all of the replicates were not able to be collected.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1131620 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1131620
completed
Timothy M. Shank
Woods Hole Oceanographic Institution
5082893392
266 Woods Hole Road
WOODS HOLE
MA
02543-1041
USA
tshank@whoi.edu
pointOfContact
Jeffrey C. Drazen
University of Hawaii at Manoa
808-956-6567
Department of Oceanography, SOEST 1000 Pope Road
Honolulu
HI
96822
USA
jdrazen@hawaii.edu
pointOfContact
Paul Yancey
Whitman College
5095275926
345 Boyer Avenue Whitman College
Walla Walla
WA
993622067
USA
yancey@whitman.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sample
cruise_id
dive
site
station
core
NiWa_id
date_utc
horizon_core_cm
Fisons NA 1500 elemental analyzer
theme
None, User defined
sample identification
cruise id
dive_id
site
station
core id
date_utc
depth core
featureType
BCO-DMO Standard Parameters
HROV Nereus
Elemental Analyzer
Push Corer
instrument
BCO-DMO Standard Instruments
TN309
service
Deployment Activity
Kermadec Trench adjacent to New Zealand: approximately 37 12.75 S and 178 51.43 E to 31 51.29 S and 176 49.07 W
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Controls on Hadal Megafaunal Community Structure: a Systematic Examination of Pressure, Food Supply, and Topography
http://www.whoi.edu/hades/
Controls on Hadal Megafaunal Community Structure: a Systematic Examination of Pressure, Food Supply, and Topography
<p><em>Extracted from the NSF award abstract:</em></p>
<p>Severe technical challenges associated with the extremes of hydrostatic pressure have prevented major advances in hadal ecological studies, and relegated hadal systems to among the most poorly investigated habitats on Earth. Through this project, Hadal Ecosystems Studies (HADES) program, PIs will determine the composition and distribution of hadal species, the role of hadal pressures (piezolyte concentrations, enzyme function under pressure), food supply (distribution of POC with the abundance and biomass of trench organisms, and metabolic rates/energetic demand), and depth/topography (genetic divergence and spatial connectivity of populations) have on impacting deep-ocean community structure. This project will examine these factors using the world's first full-ocean depth hybrid remotely operated vehicle (HROV) in conjunction with the only full-ocean depth imaging lander (Hadal-Lander). This project will provide the first seafloor data and samples in one of the world's best, yet little known trenches- the Kermadec Trench (SW Pacific Ocean). Megafaunal community structure and the relationship between POC and benthic bacterial biomass will be examined as a function of depth and location by systematic high-definition imaging and sediment/faunal sampling transects from abyssal to full trench depths both along and perpendicular to the trench axis. Population genetic approaches will provide levels of genetic divergence and evolutionarily independent lineages to assess the role of depth and topography in trenches and their adjacent abyssal plain in promoting the formation of species. Physiological constraints will be investigated by examining in-situ respiration of selected fauna and tissue concentrations of such protein stabilizers as trimethylamine oxide (TMAO), and the structural adaptations of macromolecules. </p>
<p><em>Image of NEREUS Deployment Sites. [click on the image to view a larger version]</em><br /><a href="https://datadocs.bco-dmo.org/d3/data_docs/HADES/HADES_K_Deployments_20140517.png" target="_blank"><img alt="" src="https://datadocs.bco-dmo.org/d3/data_docs/HADES/HADES_K_Deployments_20140517.png" /></a></p>
HADES
largerWorkCitation
project
eng; USA
biota
oceans
Kermadec Trench adjacent to New Zealand: approximately 37 12.75 S and 178 51.43 E to 31 51.29 S and 176 49.07 W
-178.961227
-177.290253
-35.905159
-31.9307423
2014-05-02
2014-05-07
Kermadec Trench adjacent to New Zealand: approximately 37 12.75 S and 178 51.43 E to 31 51.29 S and 176 49.07 W
0
BCO-DMO catalogue of parameters from Sample log for HADESK bacterial biomarker phospholipid fatty acid content from sediment cores collected on R/V Thomas G. Thompson cruise TN309, May 2014
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/764010.rdf
Name: sample
Units: unitless
Description: sample identifier
http://lod.bco-dmo.org/id/dataset-parameter/764011.rdf
Name: cruise_id
Units: unitless
Description: cruise identifier
http://lod.bco-dmo.org/id/dataset-parameter/764012.rdf
Name: dive
Units: unitless
Description: dive identifier
http://lod.bco-dmo.org/id/dataset-parameter/764013.rdf
Name: site
Units: unitless
Description: site name
http://lod.bco-dmo.org/id/dataset-parameter/764014.rdf
Name: station
Units: unitless
Description: station identifier
http://lod.bco-dmo.org/id/dataset-parameter/764015.rdf
Name: core
Units: unitless
Description: core identifier
http://lod.bco-dmo.org/id/dataset-parameter/764016.rdf
Name: NiWa_id
Units: unitless
Description: National Institute for Water and Atmosphere identifier
http://lod.bco-dmo.org/id/dataset-parameter/764017.rdf
Name: date_utc
Units: unitless
Description: sample collection date in UTC
http://lod.bco-dmo.org/id/dataset-parameter/764018.rdf
Name: horizon_core_cm
Units: centimeters
Description: depth horizon in core
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3992
https://darchive.mblwhoilibrary.org/bitstream/1912/24273/1/dataset-763873_hades-k-bacterial-biomarker-phospholipid-fatty-acid-plfa__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.763873.1
download
onLine
dataset
<p>Push cores (6.35 cm diameter) of sediment were collected in situ using the manipulator arm of the Hybrid Remotely Operated Vehicle Nereus on the margin of the Kermadec Trench (6013m) and along the main trench axis (7137 to 9177m) at roughly 1000m increments.</p>
<p>Cores were sectioned at 1cm intervals and sieved for the meiofaunal fraction.</p>
<p>Upon retrieval of sediment cores, the 0-1, 1-2, 2-3, 3-5, 5-10 cm depth horizons were sectioned and stored frozen (-80 °C) until analysis. Benthic bacterial biomass were calculated from sediment concentrations of the bacterial biomarker phospholipid fatty acids (PLFAs) i14:0, i15:0, ai15:0, i16:0. In brief, PLFAs were extracted and purified from known quantities of freeze-dried sediment samples and subsequently derivatized to yield fatty acid methyl esters (FAMEs). FAMEs were quantified using GC-FID (Agilent Technologies 6890N). Bacterial biomass was calculated using the well-established PLFA:C conversion factor of 0.056 gC PLFA/gC biomass.</p>
<p>Attempts were made to obtain 3 replicate cores at each depth horizon (every 1000 meters from 6000m to 10,000m.&nbsp; For logistical reasons with vehicle performance (and loss at 10,000), all of the replicates were not able to be collected.</p>
Specified by the Principal Investigator(s)
<p><strong>BCO-DMO Processing Notes:</strong><br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- re-formatted date from m/d/yyyy to yyyy-mm-dd</p>
<p>&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: Instrument Name: HROV Nereus Instrument Short Name:HROV Nereus Instrument Description: Nereus is an efficient, multi-purpose “hybrid” vehicle that can explore and operate in the crushing pressures of the greatest ocean depths. An unmanned vehicle, Nereus operates in two complementary modes. It can swim freely as an autonomous underwater vehicle (AUV) to survey large areas of the depths, map the seafloor, and give scientists a broad overview. When Nereus locates something interesting, the vehicle’s support team can bring the vehicle back on board the ship and transforms it into a remotely operated vehicle (ROV) tethered to the ship via a micro-thin, fiber-optic cable. Through this tether, Nereus can transmit high-quality, real-time video images and receive commands from skilled pilots on the ship to collect samples or conduct experiments with a manipulator arm.
Technical specifications:
Weight on land: 2,800 kg
Payload capacity: 25 kg
Maximum speed: 3 knots
Batteries: rechargable lithium ion, 15 kilowatt hours in two pressure housings
Thrusters: 2 fore and aft, 2 vertical, 1 lateral (ROV mode) 2 fore and aft, 1 vertical (AUV mode)
Lights: variable output LED array, strobes
Manipulator arm: Kraft TeleRobotics 7-function hydraulic manipulator
Sonar: scanning sonar, forward look and profile, 675 KHz
Sensors: magnetometer, CTD (to measure conductivity, temperature, and depth)
Nereus supports a variety of science operations: Push coring, measuring heat flow, geotechnical and geochemical sensing, rock sampling and drilling, biological sampling, water sampling, high resolution acoustic bathymetry, and optical still and video imagery.
More information is available from the operator site at URL.
Fisons NA 1500 elemental analyzer
Fisons NA 1500 elemental analyzer
PI Supplied Instrument Name: Fisons NA 1500 elemental analyzer Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
PI Supplied Instrument Name: PI Supplied Instrument Description:Push cores were borrowed from the National Deep Submergence Facility to be able to standardize results. The push cores were 6.35cm diameter). Instrument Name: Push Corer Instrument Short Name: Instrument Description: Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.
Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/
Cruise: TN309
TN309
R/V Thomas G. Thompson
Community Standard Description
International Council for the Exploration of the Sea
R/V Thomas G. Thompson
vessel
TN309
Timothy M. Shank
Woods Hole Oceanographic Institution
R/V Thomas G. Thompson
Community Standard Description
International Council for the Exploration of the Sea
R/V Thomas G. Thompson
vessel