Accessing BCO-DMO data
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BCO-DMO ERDDAP
Accessing BCO-DMO data |
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| qPCR_Parameters | Probe | Forward_primer | Reverse_primer | Cycling_conditions | Efficiency | Limit_of_Detection_template | Limit_of_Detection_sample | Number_plates_run | Reference |
|---|---|---|---|---|---|---|---|---|---|
| unitless | unitless | unitless | unitless | unitless | unitless | copies per microliter of template | copies per liter of sample | count | unitless |
| Bacterial 16S rRNA | TM1389F; CTTGTACACACCGCCCGTC | BACT1369F; CGGTGAATACGTTCYCGG | PROK1492R; GGWTACCTTGTTACGACTT | 94 C 2 m 35 cycles of: 94 C 15 s; 56 C 1 m; Acquisition at 56 C | 102.0 | 9.9 | 920-7000 | 3 | Suzuki et al. 2000 |
| Thaumarchaeal 16S rRNA | TM519AR; TTACCGCGGCGGCTGGCAC | GI_334F; AGATGGGTACTGAGACACGGAC | GI_554R; CTGTAGGCCCAATAATCATCCT | 94 C 2 m 40 cycles of: 94 C 15 s; 59 C 1 m; Acquisition at 59 C | 93.3 | 4.4 | 420-3200 | 2 | Suzuki et al. 2000 |
| WCA amoA | WCA-amoA-P; ACTCCGCCGAACAGTATCA | WCA-amoA-F; ACACCAGTTTGGCTWCCDTCAGC | WCA-amoA-R; TCAGCCACHGTGATCAAATTG | 95 C 10 m 40 cycles of: 95 C 15 s; 56 C 1 m; Acquisition at 56 C | 98.4 | 2.8 | 270-1100 | 3 | Mosier and Francis 2011 |
| WCB amoA | WCB-amoA-P; CCAAAGAATATYAGCGARTG | Arch-amoAFB; CATCCRATGTGGATTCCATCDTG | WCB-amoA-R; AAYGCAGTTTCTAGYGGATC | 95 C 10 m 40 cycles of: 95 C 15 s; 56 C 1 m; Acquisition at 56 C | 95.3 | 8.2 | 780-3100 | 5 | Mosier and Francis 2011; Beman et al. 2008 |
| Nitrospina 16S rRNA | NitSSU_130F; GGGTGAGTAACACGTGAATAA | NitSSU_282R; TCAGGCCGGCTAAMCA | 94 C 15 m 50 cycles of: 84 C 15 s; 57.5 C 15 s; 72 C 30 s; Acquisition at 80 C | 92.8 | 3.8 | 360-2700 | 2 | Mincer et al. 2007 |