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| Dataset Title: | [Series 3A: photophysiology] - Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - photophysiology measurements (Collaborative Research: Effects of multiple stressors on Marine Phytoplankton) 
|
| Institution: | BCO-DMO (Dataset ID: bcodmo_dataset_771461) |
| Information: | Summary
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| ISO 19115
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Attributes {
s {
CO2_expt {
Int16 _FillValue 32767;
Int16 actual_range 410, 1000;
String bcodmo_name "brief_desc";
String description "Indicates the experiment CO2 level: 410/750/1000 ppm";
String long_name "CO2 Expt";
String units "unitless";
}
Phase {
String bcodmo_name "sample_descrip";
String description "Indicates whether the sample was collected during the acclimation phase or the experiment phase of the experiment.";
String long_name "Phase";
String units "unitless";
}
CO2 {
Int16 _FillValue 32767;
Int16 actual_range 410, 1000;
String bcodmo_name "pCO2";
String description "Indicates the concentration of CO2 in the CO2-Air mix that was bubbled through the samples over the course of the experiment";
String long_name "CO2";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/";
String units "parts per million (ppm)";
}
Temperature {
String bcodmo_name "temperature";
String description "Indicates the temperature at which the samples were incubated.";
String long_name "Temperature";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/";
String units "degrees Celsius";
}
Day {
String bcodmo_name "days";
String description "Indicates the timepoint (day) of sampling. D0 = day 0; D1 = day 1; etc.";
String long_name "Day";
String units "unitless";
}
Replicate {
Byte _FillValue 127;
String _Unsigned "false";
Byte actual_range 1, 3;
String bcodmo_name "replicate";
String description "Indicates replication within a treatment. \"NA\" indicates \"not applicable\"";
String long_name "Replicate";
String units "unitless";
}
Irradiance {
String bcodmo_name "treatment";
String description "Irradiance level: SOL = sub-optimum light; OL = optimum light; EL = extreme light";
String long_name "Irradiance";
String units "unitless";
}
Fo {
Int16 _FillValue 32767;
Int16 actual_range 95, 23180;
String bcodmo_name "fluorescence";
String description "minimum fluorescence in dark-adapted state.";
String long_name "Fo";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Fm {
Int32 _FillValue 2147483647;
Int32 actual_range 124, 67785;
String bcodmo_name "fluorescence";
String description "the maximum fluorescence in dark-adapted state; measured during the first saturation flash after dark adaptation";
String long_name "FM";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Fm_L1 {
Int32 _FillValue 2147483647;
Int32 actual_range 121, 67753;
String bcodmo_name "fluorescence";
String description "The first measurement of the maximum fluorescence following exposure to actinic light at 10 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FM L1";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Fm_L2 {
Int32 _FillValue 2147483647;
Int32 actual_range 125, 69931;
String bcodmo_name "fluorescence";
String description "The second measurement of the maximum fluorescence following exposure to actinic light at 20 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FM L2";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Fm_L3 {
Int32 _FillValue 2147483647;
Int32 actual_range 89, 71719;
String bcodmo_name "fluorescence";
String description "The third measurement of the maximum fluorescence following exposure to actinic light at 50 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FM L3";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Fm_L4 {
Int32 _FillValue 2147483647;
Int32 actual_range 83, 73475;
String bcodmo_name "fluorescence";
String description "The fourth measurement of the maximum fluorescence following exposure to actinic light at 100 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FM L4";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Fm_L5 {
Int32 _FillValue 2147483647;
Int32 actual_range -306, 56017;
String bcodmo_name "fluorescence";
String description "The fifth measurement of the maximum fluorescence following exposure to actinic light at 300 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FM L5";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Fm_L6 {
Int32 _FillValue 2147483647;
Int32 actual_range -424, 39501;
String bcodmo_name "fluorescence";
String description "The sixth measurement of the maximum fluorescence following exposure to actinic light at 500 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FM L6";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Fm_L7 {
Int16 _FillValue 32767;
Int16 actual_range -124, 27504;
String bcodmo_name "fluorescence";
String description "The seventh measurement of the maximum fluorescence following exposure to actinic light at 1000 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FM L7";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Ft_L1 {
Int16 _FillValue 32767;
Int16 actual_range 115, 31243;
String bcodmo_name "fluorescence";
String description "The first measurement of the maximum fluorescence following exposure to actinic light at 10 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FT L1";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Ft_L2 {
Int16 _FillValue 32767;
Int16 actual_range 114, 31795;
String bcodmo_name "fluorescence";
String description "The second measurement of the maximum fluorescence following exposure to actinic light at 20 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FT L2";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Ft_L3 {
Int32 _FillValue 2147483647;
Int32 actual_range 133, 34266;
String bcodmo_name "fluorescence";
String description "The third measurement of the maximum fluorescence following exposure to actinic light at 50 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FT L3";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Ft_L4 {
Int32 _FillValue 2147483647;
Int32 actual_range 134, 37225;
String bcodmo_name "fluorescence";
String description "The fourth measurement of the maximum fluorescence following exposure to actinic light at 100 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FT L4";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Ft_L5 {
Int32 _FillValue 2147483647;
Int32 actual_range 139, 38038;
String bcodmo_name "fluorescence";
String description "The fifth measurement of the maximum fluorescence following exposure to actinic light at 300 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FT L5";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Ft_L6 {
Int16 _FillValue 32767;
Int16 actual_range 125, 31015;
String bcodmo_name "fluorescence";
String description "The sixth measurement of the maximum fluorescence following exposure to actinic light at 500 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FT L6";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
Ft_L7 {
Int16 _FillValue 32767;
Int16 actual_range 127, 24350;
String bcodmo_name "fluorescence";
String description "The seventh measurement of the maximum fluorescence following exposure to actinic light at 1000 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "FT L7";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/CPHLPM01/";
String units "RFU (Relative Fluorscence Units)";
}
QY_max {
Float32 _FillValue NaN;
Float32 actual_range -2.02, 0.74;
String bcodmo_name "Fv2Fm";
String description "The maximum Quantum yield. A measure of the Photosystem II efficiency. In a dark-adapted sample this is equivalent to Fv/Fm. In a light-adapted sample it is equivalent to Fv’/Fm’.";
String long_name "QY Max";
String units "unitless";
}
QY_L1 {
Float32 _FillValue NaN;
Float32 actual_range -4.72, 0.62;
String bcodmo_name "Fv2Fm";
String description "The first measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 10 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "QY L1";
String units "unitless";
}
QY_L2 {
Float32 _FillValue NaN;
Float32 actual_range -4.34, 0.62;
String bcodmo_name "Fv2Fm";
String description "The second measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 20 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "QY L2";
String units "unitless";
}
QY_L3 {
Float32 _FillValue NaN;
Float32 actual_range -6.46, 0.58;
String bcodmo_name "Fv2Fm";
String description "The third measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 50 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "QY L3";
String units "unitless";
}
QY_L4 {
Float32 _FillValue NaN;
Float32 actual_range -7.19, 0.54;
String bcodmo_name "Fv2Fm";
String description "The fourth measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 100 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "QY L4";
String units "unitless";
}
QY_L5 {
Float32 _FillValue NaN;
Float32 actual_range 0.05, 4.4;
String bcodmo_name "Fv2Fm";
String description "The fifth measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 300 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "QY L5";
String units "unitless";
}
QY_L6 {
Float32 _FillValue NaN;
Float32 actual_range 0.07, 3.37;
String bcodmo_name "Fv2Fm";
String description "The sixth measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 500 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "QY L6";
String units "unitless";
}
QY_L7 {
Float32 _FillValue NaN;
Float32 actual_range -0.07, 6.0;
String bcodmo_name "Fv2Fm";
String description "The seventh measurement of the instantenous photosystem II quantum yield following exposure to actinic light at 1000 micro-mol photons·m-2·sec-1 for 60 seconds (L1 indicates the first measurement in the \"light\" phase)";
String long_name "QY L7";
String units "unitless";
}
}
NC_GLOBAL {
String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv";
String acquisition_description
"Three CO2 concentrations were tested: 410 ppm, 750 ppm, and 1000 ppm
respectively. For each CO2 concentration, four temperatures were tested: 15
degrees-C, 20 degrees-C, 25 degrees-C, and 30 degrees-C. Within each
temperature, three light levels were tested: a sub-optimum light (SOL)
intensity of 60 umol photons \\u00b7 m-2 \\u00b7 s-1, an optimum light (OL)
intensity of 400 umol photons \\u00b7 m-2 \\u00b7 s-1 and an extreme light (EL)
intensity of 800 umol photons \\u00b7 m-2 \\u00b7 s-1. All lights were set at a
12 h day: 12 h dark cycle. For logistical reasons, experiments were partially
conducted in series, with all light treatments at two temperatures (either 15
degrees-C and 25 degrees-C or 20 degrees-C and 30 degrees-C) running
simultaneously. This was repeated for each CO2 concentration.
Experiments were conducted in Multicultivator MC-1000 OD units (Photon Systems
Instruments, Drasov, Czech Republic). Each unit consists of eight 85 ml test-
tubes immersed in a thermostated water bath, each independently illuminated by
an array of cool white LEDs set at specific intensity and timing. A 0.2um
filtered CO2-air mix (Praxair Distribution Inc.) was bubbled through sterile
artificial seawater, and the humidified gas mix was supplied to each tube via
gentle sparging through a 2um stainless steel diffuser. Flow rates were
gradually increased over the course of the incubation to compensate for the
DIC uptake of actively growing cells, and ranged from <0.04 Liters per minute
(LPM) at the start of the incubations to 0.08 LPM in each tube after 2 days.
For each CO2 and temperature level, replication was achieved by incubating
three tubes at sub-optimum light intensities, two tubes at optimum light
intensity, and three tubes at extreme light intensities. Each experiment was
split into two phases: An acclimation phase spanning 4 days, was used to
acclimate cultures to their new environment. Pre-acclimated, exponentially-
growing cultures were then inoculated into fresh media and incubated through a
3-day experimental phase during which assessments of growth, photophysiology,
and nutrient cycling were carried out daily. All sampling started 5 hours into
the daily light cycle to minimize the effects of diurnal cycles.
Experiments were conducted with artificial seawater (ASW) prepared using
previously described methods (Kester et. al 1967), and enriched with nitrate
(NO3), phosphate (PO4), silicic acid (Si[OH]4), at levels ensuring that the
cultures would remain nutrient-replete over the course of the experiment.
Trace metals and vitamins were added as in f/2 (Guillard 1975). The expected
DIC concentration and pH of the growth media was determined for the different
pCO2 and temperatures using the CO2SYS calculator (Pierrot et al. 2006), with
constants from Mehrbach et al. (1973, refit by Dickson & Millero 1987), and
inputs of temperature, salinity, total alkalinity (2376.5 umol \\u00b7 kg-1),
pCO2, phosphate, and silicic acid. DIC levels in ASW at the start of each
phase of the experiments were manipulated by the addition of NaHCO3, and was
then maintained by bubbling a CO2-Air mix through the cultures over the course
of the experiments. The pH of the growth media was measured
spectrophometrically using the m-cresol purple method (Dickson 1993), and
adjusted using 0.1N HCl or 0.1M NaOH. The media was distributed into 75 ml
aliquots and each aliquot was inoculated with 5 ml of the T. pseudonana CCMP
1014 (TP1014) stock culture at the start of the experiments.
Photophysiology:
Photophysiology was assessed daily using a handheld Pulse Amplitude
Modulated (PAM) fluorometer (AquaPen-C AP-C 100, Photon System Instruments,
Czech Republic). A sample was collected from each light treatment for each, 5
hours after the start of the daily light cycle, and placed in the dark for a
minimum of 30 minutes prior to measurements. The dark-adapted sample was used
to generate light curves that provide measurements of in-vivo chlorophyll
autofluorescence (F0), the maximum quantum yield (QYmax or Fv/Fm), and
relative photosynthesis rates based on PSII quantum yields at varying light
intensities - using the instrument\\u2019s LC3 protocol. The LC3 protocol
involves measurements of baseline and maximal fluorescence over seven
60-second phases, with each phase representing a light intensity from 10 to
1000 \\u03bcmol photons m-2 \\u00b7 s-1.\\u00a0 Blue light (455 nm) was used as
actinic light in these experiments, and measurements were made at measuring
illumination (f-pulse) intensity of 0.03 \\u03bcmol photons m-2 \\u00b7 s-1, and
saturating (F-pulse) illumination of 2100\\u2009micro-mol photons m-2 \\u00b7
s-1, and actinic illumination (A-pulse) controlled by the instrument's
protocol were set at 10, 20, 50, 100, 300, 500, and 1000 micro-mol photons m-2
\\u00b7 s-1 (for each 60-second phase).";
String awards_0_award_nid "654346";
String awards_0_award_number "OCE-1538602";
String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1538602";
String awards_0_funder_name "NSF Division of Ocean Sciences";
String awards_0_funding_acronym "NSF OCE";
String awards_0_funding_source_nid "355";
String awards_0_program_manager "Michael E. Sieracki";
String awards_0_program_manager_nid "50446";
String cdm_data_type "Other";
String comment
"Series 3A-3: Multiple stressor experiments on T. pseudonana (CCMP1014): photophysiology
PI: U. Passow, N. D'Souza (UCSB), E. Laws (LSU)
version date: 2019-06-17";
String Conventions "COARDS, CF-1.6, ACDD-1.3";
String creator_email "info@bco-dmo.org";
String creator_name "BCO-DMO";
String creator_type "institution";
String creator_url "https://www.bco-dmo.org/";
String data_source "extract_data_as_tsv version 2.3 19 Dec 2019";
String dataset_current_state "Final and no updates";
String date_created "2019-06-20T13:24:12Z";
String date_modified "2020-06-29T19:08:12Z";
String defaultDataQuery "&time<now";
String doi "10.26008/1912/bco-dmo.771461.1";
String history
"2024-12-03T17:17:18Z (local files)
2024-12-03T17:17:18Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_771461.html";
String infoUrl "https://www.bco-dmo.org/dataset/771461";
String institution "BCO-DMO";
String instruments_0_acronym "Fluorometer";
String instruments_0_dataset_instrument_description "Used for assessment of photochemistry.";
String instruments_0_dataset_instrument_nid "771562";
String instruments_0_description "A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.";
String instruments_0_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/113/";
String instruments_0_instrument_name "Fluorometer";
String instruments_0_instrument_nid "484";
String instruments_0_supplied_name "hand-held Aquapen-C AP-C 100 (Photon Systems Instruments)";
String instruments_1_acronym "Spectrophotometer";
String instruments_1_dataset_instrument_description "Used to measure pH.";
String instruments_1_dataset_instrument_nid "771470";
String instruments_1_description "An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples.";
String instruments_1_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB20/";
String instruments_1_instrument_name "Spectrophotometer";
String instruments_1_instrument_nid "707";
String instruments_1_supplied_name "Genesys 10SVIS spectrophotometer";
String instruments_2_dataset_instrument_description "Used for incubation of TP1014 cultures.";
String instruments_2_dataset_instrument_nid "771469";
String instruments_2_description "An instrument used for the purpose of culturing small cells such as algae or bacteria. May provide temperature and light control and bubbled gas introduction.";
String instruments_2_instrument_name "Cell Cultivator";
String instruments_2_instrument_nid "714540";
String instruments_2_supplied_name "Multicultivator MC-1000 OD (Qubit Systems)";
String keywords "bco, bco-dmo, biological, carbon, carbon dioxide, chemical, co2, CO2_expt, data, dataset, day, dioxide, dmo, erddap, expt, Fm_L1, Fm_L2, Fm_L3, Fm_L4, Fm_L5, Fm_L6, Fm_L7, Ft_L1, Ft_L2, Ft_L3, Ft_L4, Ft_L5, Ft_L6, Ft_L7, irradiance, L2, L3, L4, management, max, oceanography, office, phase, preliminary, QY_L1, QY_L2, QY_L3, QY_L4, QY_L5, QY_L6, QY_L7, QY_max, replicate, temperature";
String license "https://www.bco-dmo.org/dataset/771461/license";
String metadata_source "https://www.bco-dmo.org/api/dataset/771461";
String param_mapping "{'771461': {}}";
String parameter_source "https://www.bco-dmo.org/mapserver/dataset/771461/parameters";
String people_0_affiliation "University of California-Santa Barbara";
String people_0_affiliation_acronym "UCSB-MSI";
String people_0_person_name "Uta Passow";
String people_0_person_nid "51317";
String people_0_role "Principal Investigator";
String people_0_role_type "originator";
String people_1_affiliation "Louisiana State University";
String people_1_affiliation_acronym "LSU-SC&E";
String people_1_person_name "Dr Edward Laws";
String people_1_person_nid "50767";
String people_1_role "Co-Principal Investigator";
String people_1_role_type "originator";
String people_2_affiliation "University of California-Santa Barbara";
String people_2_affiliation_acronym "UCSB-MSI";
String people_2_person_name "Nigel D'Souza";
String people_2_person_nid "748936";
String people_2_role "Scientist";
String people_2_role_type "originator";
String people_3_affiliation "University of California-Santa Barbara";
String people_3_affiliation_acronym "UCSB-MSI";
String people_3_person_name "Nigel D'Souza";
String people_3_person_nid "748936";
String people_3_role "Contact";
String people_3_role_type "related";
String people_4_affiliation "Woods Hole Oceanographic Institution";
String people_4_affiliation_acronym "WHOI BCO-DMO";
String people_4_person_name "Nancy Copley";
String people_4_person_nid "50396";
String people_4_role "BCO-DMO Data Manager";
String people_4_role_type "related";
String project "Stressors on Marine Phytoplankton";
String projects_0_acronym "Stressors on Marine Phytoplankton";
String projects_0_description
"The overarching goal of this project is to develop a framework for understanding the response of phytoplankton to multiple environmental stresses. Marine phytoplankton, which are tiny algae, produce as much oxygen as terrestrial plants and provide food, directly or indirectly, to all marine animals. Their productivity is thus important both for global elemental cycles of oxygen and carbon, as well as for the productivity of the ocean. Globally the productivity of marine phytoplankton appears to be changing, but while we have some understanding of the response of phytoplankton to shifts in one environmental parameter at a time, like temperature, there is very little knowledge of their response to simultaneous changes in several parameters. Increased atmospheric carbon dioxide concentrations result in both ocean acidification and increased surface water temperatures. The latter in turn leads to greater ocean stratification and associated changes in light exposure and nutrient availability for the plankton. Recently it has become apparent that the response of phytoplankton to simultaneous changes in these growth parameters is not additive. For example, the effect of ocean acidification may be severe at one temperature-light combination and negligible at another. The researchers of this project will carry out experiments that will provide a theoretical understanding of the relevant interactions so that the impact of climate change on marine phytoplankton can be predicted in an informed way. This project will engage high schools students through training of a teacher and the development of a teaching unit. Undergraduate and graduate students will work directly on the research. A cartoon journalist will create a cartoon story on the research results to translate the findings to a broader general public audience.
Each phytoplankton species has the capability to acclimatize to changes in temperature, light, pCO2, and nutrient availability - at least within a finite range. However, the response of phytoplankton to multiple simultaneous stressors is frequently complex, because the effects on physiological responses are interactive. To date, no datasets exist for even a single species that could fully test the assumptions and implications of existing models of phytoplankton acclimation to multiple environmental stressors. The investigators will combine modeling analysis with laboratory experiments to investigate the combined influences of changes in pCO2, temperature, light, and nitrate availability on phytoplankton growth using cultures of open ocean and coastal diatom strains (Thalassiosira pseudonana) and an open ocean cyanobacteria species (Synechococcus sp.). The planned experiments represent ideal case studies of the complex and interactive effects of environmental conditions on organisms, and results will provide the basis for predictive modeling of the response of phytoplankton taxa to multiple environmental stresses.";
String projects_0_end_date "2018-09";
String projects_0_name "Collaborative Research: Effects of multiple stressors on Marine Phytoplankton";
String projects_0_project_nid "654347";
String projects_0_start_date "2015-10";
String publisher_name "Biological and Chemical Oceanographic Data Management Office (BCO-DMO)";
String publisher_type "institution";
String sourceUrl "(local files)";
String standard_name_vocabulary "CF Standard Name Table v55";
String summary "The experiments were designed to test the combined effects of CO2, temperatures, and light on the growth of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements of photophysiology using the Light curve (LC3) protocol of the Aquapen-C AP-C 100 fluorometer.";
String title "[Series 3A: photophysiology] - Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - photophysiology measurements (Collaborative Research: Effects of multiple stressors on Marine Phytoplankton)";
String version "1";
String xml_source "osprey2erddap.update_xml() v1.5";
}
}
Data Access Protocol (DAP) and its
selection constraints.
The URL specifies what you want: the dataset, a description of the graph or the subset of the data, and the file type for the response.
Tabledap request URLs must be in the form
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/datasetID.fileType{?query}
For example,
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/pmelTaoDySst.htmlTable?longitude,latitude,time,station,wmo_platform_code,T_25&time>=2015-05-23T12:00:00Z&time<=2015-05-31T12:00:00Z
Thus, the query is often a comma-separated list of desired variable names,
followed by a collection of
constraints (e.g., variable<value),
each preceded by '&' (which is interpreted as "AND").
For details, see the tabledap Documentation.