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Dataset Title:	[cal_iso] - Calibration of the Isotope Ratio Mass Spectrometer vs. aliquots of 15N2 gas diluted in air then helium (EAGER: Collaborative Research: Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean)
Institution:	BCO-DMO (Dataset ID: bcodmo_dataset_778000)
Information:	Summary \| License \| ISO 19115 \| Metadata \| Background \| Files \| Make a graph

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Variable	Minimum	Maximum
comment (unitless)	"NaN"	"air"
expected (unitless)	0.00134689	10.0
measured (unitless)	0.00329868	10.1

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The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  comment {
    String bcodmo_name "comment";
    String description "sample type";
    String long_name "Comment";
    String units "unitless";
  }
  expected {
    Float32 _FillValue NaN;
    Float32 actual_range 0.00134689, 10.0;
    String bcodmo_name "unknown";
    String description "Expceted 15N2 air He dil (m/z 30/28 ratio x 100)";
    String long_name "Expected";
    String units "unitless";
  }
  measured {
    Float32 _FillValue NaN;
    Float32 actual_range 0.00329868, 10.1;
    String bcodmo_name "unknown";
    String description "measured  m/z 30/28 ratio x 100 on GC-IRMS";
    String long_name "Measured";
    String units "unitless";
  }
 }
  NC_GLOBAL {
    String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv";
    String acquisition_description 
"A serial dilution of 98-99% 15N2 gas stock (Cambridge Isotope Laboratories,
Lot #I-21065) in air and helium returned the expected signal (i.e., a slope
not significantly different than unity) on a continuous flow Delta V Advantage
Plus IRMS (Smith et al. 2015), demonstrating the accuracy of the IRMS over a
wide range of 15N2 abundances.";
    String awards_0_award_nid "772538";
    String awards_0_award_number "OCE-1732246";
    String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1732246";
    String awards_0_funder_name "NSF Division of Ocean Sciences";
    String awards_0_funding_acronym "NSF OCE";
    String awards_0_funding_source_nid "355";
    String awards_0_program_manager "Henrietta N Edmonds";
    String awards_0_program_manager_nid "51517";
    String cdm_data_type "Other";
    String comment 
"Calibration of the Isotope Ratio Mass Spectrometer vs. aliquots of 15N2 gas diluted in air then helium 
  PI: Julie Granger 
  Version: 2019-09-30";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_type "institution";
    String creator_url "https://www.bco-dmo.org/";
    String data_source "extract_data_as_tsv version 2.3  19 Dec 2019";
    String date_created "2019-09-30T13:13:59Z";
    String date_modified "2019-10-02T18:47:08Z";
    String defaultDataQuery "&amp;time&lt;now";
    String doi "10.1575/1912/bco-dmo.778000.1";
    String history 
"2024-11-21T09:14:24Z (local files)
2024-11-21T09:14:24Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_778000.html";
    String infoUrl "https://www.bco-dmo.org/dataset/778000";
    String institution "BCO-DMO";
    String instruments_0_acronym "IR Mass Spec";
    String instruments_0_dataset_instrument_description "continuous flow Delta V Isotope Ratio Mass Spectrometer (Smith et al. 2015), and continuous flow-GV Isoprime IRMS (Charoenpong et al., 2014)";
    String instruments_0_dataset_instrument_nid "778016";
    String instruments_0_description "The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).";
    String instruments_0_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB16/";
    String instruments_0_instrument_name "Isotope-ratio Mass Spectrometer";
    String instruments_0_instrument_nid "469";
    String instruments_0_supplied_name "Isotope Ratio Mass Spectrometer";
    String instruments_1_acronym "MIMS";
    String instruments_1_dataset_instrument_description "Membrane Inlet Mass Spectrometer (Bay Instruments)";
    String instruments_1_dataset_instrument_nid "778015";
    String instruments_1_description "Membrane-introduction mass spectrometry (MIMS) is a method of introducing analytes into the mass spectrometer's vacuum chamber via a semipermeable membrane.";
    String instruments_1_instrument_name "Membrane Inlet Mass Spectrometer";
    String instruments_1_instrument_nid "661606";
    String instruments_1_supplied_name "Membrane Inlet Mass Spectrometer";
    String keywords "bco, bco-dmo, biological, chemical, comment, data, dataset, dmo, erddap, expected, management, measured, oceanography, office, preliminary";
    String license "https://www.bco-dmo.org/dataset/778000/license";
    String metadata_source "https://www.bco-dmo.org/api/dataset/778000";
    String param_mapping "{'778000': {}}";
    String parameter_source "https://www.bco-dmo.org/mapserver/dataset/778000/parameters";
    String people_0_affiliation "University of Connecticut";
    String people_0_affiliation_acronym "UConn";
    String people_0_person_name "Julie Granger";
    String people_0_person_nid "528937";
    String people_0_role "Principal Investigator";
    String people_0_role_type "originator";
    String people_1_affiliation "University of Massachusetts Dartmouth";
    String people_1_affiliation_acronym "UMass Dartmouth";
    String people_1_person_name "Annie Bourbonnais";
    String people_1_person_nid "778011";
    String people_1_role "Co-Principal Investigator";
    String people_1_role_type "originator";
    String people_2_affiliation "University of Hawaii";
    String people_2_person_name "Samuel Wilson";
    String people_2_person_nid "51733";
    String people_2_role "Co-Principal Investigator";
    String people_2_role_type "originator";
    String people_3_affiliation "Woods Hole Oceanographic Institution";
    String people_3_affiliation_acronym "WHOI BCO-DMO";
    String people_3_person_name "Mathew Biddle";
    String people_3_person_nid "708682";
    String people_3_role "BCO-DMO Data Manager";
    String people_3_role_type "related";
    String project "EAGER NitFix";
    String projects_0_acronym "EAGER NitFix";
    String projects_0_description 
"NSF Award Abstract:
The availability of nitrogen is required to support the growth and production of organisms living in the surface of our global ocean. This element can be scarce. To alleviate this scarcity, a special class of bacteria and archaea, called nitrogen fixers, can derive the nitrogen needed for growth from nitrogen gas. This project would carefully examine one specific method for measuring nitrogen fixation that has been used recently to suggest the occurrence of small amounts of nitrogen fixation in subsurface ocean waters. If these reports are verified, then a revision of our understanding of the marine nitrogen cycle may be needed. The Ocean Carbon and Biogeochemistry program will be used as a platform to develop community consensus for best practices in nitrogen fixation measurements and detection of diversity, activity, and abundances of the organisms responsible. In addition, a session will be organized in a future national/international conference to communicate with the broader scientific community while developing these best practices.
The goal of this study is to conduct a thorough examination of potential experimental and analytical errors inherent to the 15N2-tracer nitrogen fixation method, in tandem with comprehensive molecular measurements, in mesopelagic ocean waters. Samples will be collected and experimental work conducted on a cruise transect in the North Atlantic Ocean, followed by analytical work in the laboratory. The specific aims of this study are to (1) determine the minimum quantifiable rates of 15N2 fixation based on incubations of mesopelagic waters via characterization of sources of experimental and analytical error, and (2) seek evidence of presence and expression of nitrogen fixation genes via comprehensive molecular approaches on corresponding samples. The range of detectable rates and diazotroph activity from the measurements made in this study will be informative for the understanding of the importance of nitrogen fixation in the oceanic nitrogen budget.";
    String projects_0_end_date "2018-10";
    String projects_0_geolocation "North Atlantic Ocean, Pacific Ocean";
    String projects_0_name "EAGER:  Collaborative Research:  Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean";
    String projects_0_project_nid "772534";
    String projects_0_start_date "2017-05";
    String publisher_name "Biological and Chemical Oceanographic Data Management Office (BCO-DMO)";
    String publisher_type "institution";
    String sourceUrl "(local files)";
    String standard_name_vocabulary "CF Standard Name Table v55";
    String summary "In order to verify that measurements of the 15N2 atom% of dissolved N2 gas samples effectuated by Membrane Inlet Mass Spectrometry are accurate, we compared these to corresponding measurements of parallel samples on a Delta V Isotope Ratio Mass Spectrometer (IRMS). To this end, we first ensured that the IRMS returns accurate 15N2 atom% values, by measuring the 15N2 atom% of a serial dilutions of a 15N2 gas stock in air and helium. This calibration is documented herein, showing that IRMS measurements in the experimental range were accurate, as expected";
    String title "[cal_iso] - Calibration of the Isotope Ratio Mass Spectrometer vs. aliquots of 15N2 gas diluted in air then helium (EAGER:  Collaborative Research:  Detection limit in marine nitrogen fixation measurements - Constraints of rates from the mesopelagic ocean)";
    String version "1";
    String xml_source "osprey2erddap.update_xml() v1.3";
  }
}

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