http://lod.bco-dmo.org/id/dataset/779425
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2019-10-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Oxygen metrics for retinal function in marine invertebrate larvae determined with electroretinograms
2019-10-23
publication
2019-10-23
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-11-01
publication
https://doi.org/10.1575/1912/bco-dmo.779425.1
Lisa A. Levin
University of California-San Diego
principalInvestigator
Nicholas Oesch
University of California-San Diego
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Levin, L., Oesch, N. (2019) Oxygen metrics for retinal function in marine invertebrate larvae determined with electroretinograms. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-10-23 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.779425.1 [access date]
Dataset Description: <p>Oxygen metrics for retinal function in marine invertebrate larvae determined with electroretinograms.</p>
<p>Please see additional datasets for this paper, including the “RetinalResponse TimeSeries” and the “Experiment Metrics” datasets.</p> Methods and Sampling: <p>Detailed methods can be found in McCormick, LR; Levin, LA; Oesch, NW. 2019. Vision is highly sensitive to oxygen availability in marine invertebrate larvae. Journal of Experimental Biology 222, jeb200899. doi:10.1242/jeb.200899. Data shown in Figure 1, bottom panel.</p>
<p>Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 μmol photons m<strong>−</strong>2 s<strong>−</strong>1 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in “normoxia” (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution. This dataset shows the three visual metrics that were calculated to quantify the effects of reduced pO2 on retinal function. V90, V50, and V10 were calculated for each individual larva as the oxygen where there was 90%, 50%, and 10% retinal function remaining, in respect to retinal responses in normoxia (surface-ocean oxygen levels).</p>
<p>Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829623 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1829623
onGoing
Lisa A. Levin
University of California-San Diego
858-534-3579
9500 Gilman Drive Mail Code: 0218
La Jolla
CA
92093-0218
USA
llevin@ucsd.edu
pointOfContact
Nicholas Oesch
University of California-San Diego
858-534-2991
9500 Gilman Dr.
La Jolla
CA
92093-0109
USA
noesch@mail.ucsd.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Species
Experiment_Name
Metric
O2_umol_l
O2_ml_l
O2_umol_kg
O2_pO2
theme
None, User defined
species
experiment id
No BCO-DMO term
dissolved Oxygen
featureType
BCO-DMO Standard Parameters
Oxygen Microelectrode Sensor
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae
https://www.bco-dmo.org/project/775843
Vision-mediated influence of low oxygen on the physiology and ecology of marine larvae
<p>NSF abstract:<br />
Oxygen is being lost in the ocean worldwide as a result of ocean warming and the input of nutrients from land. Vision requires a large amount of oxygen, and may be less effective or require more light when oxygen is in short supply. This is especially true for active marine animals with complex eyes and visual capabilities, including active arthropods (crabs), cephalopods (squid), and fish. The California coastal waters exhibit a sharp drop in oxygen and light with increasing water depth. This project examines how visual physiology and ecology in young (larval) highly visual marine animals respond to oxygen loss, with a focus on key fisheries and aquaculture species. Experiments and observations will test the hypothesis that oxygen stress will change the light required for these organisms to see effectively, influencing the water depths where they can live and survive. The project will provide interdisciplinary experiences to students and an early career scientist and inform both the public (through outreach at the Birch Aquarium at Scripps Institution of Oceanography) and policy makers about the effects of oxygen decline in the ocean.</p>
<p>Negative effects of oxygen loss on vision have been described for humans and other terrestrial organisms, but never in the marine environment, despite the large changes in oxygen that can occur with depth and over time in the ocean, and the high metabolic demand of visual systems. This project will test the effects of low oxygen on vision in 3 combinations of eye design and photo-transduction mechanisms: compound eye with rhabdomeric photoreceptors (arthropods), simple eye with rhabdomeric photoreceptors (cephalopods), and simple eye with ciliary photoreceptors (fish). A series of oxygen- and light-controlled laboratory experiments will be conducted on representative taxa of each group including the tuna crab, Pleuroncodes planipes; the market squid, Doryteuthis opalescens, and the white sea bass, Atractoscion nobilis. In vivo electrophysiology and behavioral phototaxis experiments will identify new oxygen metrics for visual physiology and function, and will be compared to metabolic thresholds determined in respiration experiments. Hydrographic data collected over 3 decades by the CalCOFI program in the Southern California Bight will be evaluated with respect to visual and metabolic limits to determine the consequences of oxygen variation on the critical luminoxyscape (range of oxygen and light conditions required for visual physiology and function in target species) boundary in each species. Findings for the three vision-based functional groups may test whether oxygen-limited visual responses offer an additional explanation for the shoaling of species distributions among highly visual pelagic taxa in low oxygen, and will help to focus future research efforts and better understand the stressors contributing to habitat compression with expanding oxygen loss in the ocean.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Vision under hypoxia
largerWorkCitation
project
eng; USA
oceans
2019-10-23
Southern California Bight, Northeast Pacific Ocean
0
BCO-DMO catalogue of parameters from Oxygen metrics for retinal function in marine invertebrate larvae determined with electroretinograms
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/780450.rdf
Name: Species
Units: unitless
Description: Species name.
http://lod.bco-dmo.org/id/dataset-parameter/780451.rdf
Name: Experiment_Name
Units: unitless
Description: Original experiment name that can be linked back to raw datafile collected in Igor.
http://lod.bco-dmo.org/id/dataset-parameter/780452.rdf
Name: Metric
Units: unitless
Description: One of three oxygen metrics for retinal function calculated in McCormick et al.
http://lod.bco-dmo.org/id/dataset-parameter/780453.rdf
Name: O2_umol_l
Units: micromole per liter (umol/L)
Description: Oxygen concentration in umol O2/L of the given metric.
http://lod.bco-dmo.org/id/dataset-parameter/780454.rdf
Name: O2_ml_l
Units: milliliter per liter (ml/l)
Description: Oxygen concentration in mL O2/L of the given metric.
http://lod.bco-dmo.org/id/dataset-parameter/780455.rdf
Name: O2_umol_kg
Units: micromole per kg (umol/kg)
Description: Oxygen concentration in umol O2/kg of the given metric.
http://lod.bco-dmo.org/id/dataset-parameter/780456.rdf
Name: O2_pO2
Units: kiloPascale (kPa)
Description: The partial pressure of oxygen of the given metric.
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
3745
https://darchive.mblwhoilibrary.org/bitstream/1912/24766/1/dataset-779425_oxygen-metrics__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.779425.1
download
onLine
dataset
<p>Detailed methods can be found in McCormick, LR; Levin, LA; Oesch, NW. 2019. Vision is highly sensitive to oxygen availability in marine invertebrate larvae. Journal of Experimental Biology 222, jeb200899. doi:10.1242/jeb.200899. Data shown in Figure 1, bottom panel.</p>
<p>Briefly, the time series test recorded electroretinogram (ERG) responses to a 1 s square step of light at a constant irradiance of 3.56 μmol photons m<strong>−</strong>2 s<strong>−</strong>1 repeated every 20 s, providing a nearly continuous measure of ERG response in a tethered, live larva during the experimental manipulation of partial pressure of oxygen (pO2). There was a constant flow of pH-buffered sterile seawater in the chamber where the larva was held, and after a brief period in “normoxia” (surface-ocean oxygen levels), the pO2 was decreased, and then held at a low pO2 before re-oxygenating the solution. This dataset shows the three visual metrics that were calculated to quantify the effects of reduced pO2 on retinal function. V90, V50, and V10 were calculated for each individual larva as the oxygen where there was 90%, 50%, and 10% retinal function remaining, in respect to retinal responses in normoxia (surface-ocean oxygen levels).</p>
<p>Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe.</p>
Specified by the Principal Investigator(s)
<p>All electrophysiology data was recorded and analyzed using Igor Pro 7 Software (Wavemetrics) using custom code. Oxygen data was analyzed using PreSens Measurement Studio 2. Post-processing analysis was completed in R Studio (version 3.3.3).</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
PI Supplied Instrument Name: PI Supplied Instrument Description:Oxygen was measured using a Microx4 (PreSens) oxygen meter and a Pst-7 oxygen optode probe. Instrument Name: Oxygen Microelectrode Sensor Instrument Short Name:O2 Microelectrode Instrument Description: Any microelectrode sensor that measures oxygen. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/351/