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Dataset Title:  Elemental carbon and nitrogen data for Skeletonema species as analyzed in
Anderson and Rynearson, 2020
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Institution:  BCO-DMO   (Dataset ID: bcodmo_dataset_780386)
Information:  Summary ? | License ? | ISO 19115 | Metadata | Background (external link) | Files | Make a graph
 
Variable ?   Optional
Constraint #1 ?
Optional
Constraint #2 ?
   Minimum ?
 
   Maximum ?
 
 Species (unitless) ?          "S. marinoi"    "S. pseudocostatum"
 Strain (unitless) ?          "SmarA"    "SpseE"
 GenBank (unitless) ?          "MH673580"    "MH673594"
 Collection_date (unitless) ?          "2015-03-03"    "2016-06-13"
 latitude (Isolation Lat, degrees_north) ?          41.47    41.47
  < slider >
 longitude (Isolation Lon, degrees_east) ?          -71.39    -71.39
  < slider >
 Isolation_Temperature (degrees C) ?          -0.94    22.91
 Temperature (degrees C) ?          4    33
 meanC (picomol carbon per cell) ?          0.512    1.887
 seC (picomol carbon per cell) ?          0.012    0.174
 meanN (picomol nitrogen per cell) ?          0.073    0.263
 seN (picomol nitrogen per cell) ?          0.003    0.024
 meanCN (unitless) ?          3.854    9.949
 seCN (unitless) ?          0.044    0.66
 meanV (cubic micrometers (um3)) ?          43.4    235.2
 seV (cubic micrometers (um3)) ?          4.0    20.5
 mean_cd (femtomol/cubic micrometers (fmol um-3)) ?          1.688    7.441
 se_cd (femtomol/cubic micrometers (fmol um-3)) ?          0.292    1.118
 mean_nd (femtomol/cubic micrometers (fmol um-3)) ?          0.062    1.157
 se_nd (femtomol/cubic micrometers (fmol um-3)) ?          0.017    0.11
 
Server-side Functions ?
 distinct() ?
? ("Hover here to see a list of options. Click on an option to select it.Hover here to see a list of options. Click on an option to select it.Hover here to see a list of options. Click on an option to select it.Hover here to see a list of options. Click on an option to select it.Hover here to see a list of options. Click on an option to select it.")

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The Dataset Attribute Structure (.das) for this Dataset

Attributes {
 s {
  Species {
    String bcodmo_name "species";
    String description "Species";
    String long_name "Species";
    String units "unitless";
  }
  Strain {
    String bcodmo_name "sample_descrip";
    String description "Strain";
    String long_name "Strain";
    String units "unitless";
  }
  GenBank {
    String bcodmo_name "accession number";
    String description "GenBank Accession Number associated with each strain";
    String long_name "Gen Bank";
    String units "unitless";
  }
  Collection_date {
    String bcodmo_name "date";
    String description "Date of collection from the environment; formatted as yyyy-mm-dd";
    String long_name "Collection Date";
    String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/";
    String source_name "Collection_date";
    String time_precision "1970-01-01";
    String units "unitless";
  }
  latitude {
    String _CoordinateAxisType "Lat";
    Float64 _FillValue NaN;
    Float64 actual_range 41.47, 41.47;
    String axis "Y";
    String bcodmo_name "latitude";
    String description "Latitude of strain isolation; north is positive";
    String ioos_category "Location";
    String long_name "Isolation Lat";
    String nerc_identifier "https://vocab.nerc.ac.uk/collection/P09/current/LATX/";
    String standard_name "latitude";
    String units "degrees_north";
  }
  longitude {
    String _CoordinateAxisType "Lon";
    Float64 _FillValue NaN;
    Float64 actual_range -71.39, -71.39;
    String axis "X";
    String bcodmo_name "longitude";
    String description "Longitude of strain isolation; east is positive";
    String ioos_category "Location";
    String long_name "Isolation Lon";
    String nerc_identifier "https://vocab.nerc.ac.uk/collection/P09/current/LONX/";
    String standard_name "longitude";
    String units "degrees_east";
  }
  Isolation_Temperature {
    Float32 _FillValue NaN;
    Float32 actual_range -0.94, 22.91;
    String bcodmo_name "temp_ss";
    String description "Sea surface temperature (SST) at time and position of isolation";
    String long_name "Isolation Temperature";
    String units "degrees C";
  }
  Temperature {
    Byte _FillValue 127;
    Byte actual_range 4, 33;
    String bcodmo_name "temp_incub";
    String description "Experimental temperature at which measurements were recorded";
    String long_name "Temperature";
    String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/";
    String units "degrees C";
  }
  meanC {
    Float32 _FillValue NaN;
    Float32 actual_range 0.512, 1.887;
    String bcodmo_name "C";
    String description "Mean carbon per cell";
    String long_name "Mean C";
    String units "picomol carbon per cell";
  }
  seC {
    Float32 _FillValue NaN;
    Float32 actual_range 0.012, 0.174;
    String bcodmo_name "C";
    String description "Standard error of carbon per cell";
    String long_name "Se C";
    String units "picomol carbon per cell";
  }
  meanN {
    Float32 _FillValue NaN;
    Float32 actual_range 0.073, 0.263;
    String bcodmo_name "N";
    String description "Mean nitrogen per cell";
    String long_name "Mean N";
    String units "picomol nitrogen per cell";
  }
  seN {
    Float32 _FillValue NaN;
    Float32 actual_range 0.003, 0.024;
    String bcodmo_name "N";
    String description "Standard error of nitrogen per cell";
    String long_name "Se N";
    String units "picomol nitrogen per cell";
  }
  meanCN {
    Float32 _FillValue NaN;
    Float32 actual_range 3.854, 9.949;
    String bcodmo_name "C_to_N";
    String description "Average molar ratio of carbon to nitrogen per cell";
    String long_name "Mean CN";
    String units "unitless";
  }
  seCN {
    Float32 _FillValue NaN;
    Float32 actual_range 0.044, 0.66;
    String bcodmo_name "C_to_N";
    String description "Standard error of molar carbon to nitrogen per cell";
    String long_name "Se CN";
    String units "unitless";
  }
  meanV {
    Float32 _FillValue NaN;
    Float32 actual_range 43.4, 235.2;
    String bcodmo_name "volume";
    String description "Mean cell volume";
    String long_name "Mean V";
    String units "cubic micrometers (um3)";
  }
  seV {
    Float32 _FillValue NaN;
    Float32 actual_range 4.0, 20.5;
    String bcodmo_name "volume";
    String description "Cell volume standard error";
    String long_name "Se V";
    String units "cubic micrometers (um3)";
  }
  mean_cd {
    Float32 _FillValue NaN;
    Float32 actual_range 1.688, 7.441;
    String bcodmo_name "C";
    String description "Mean carbon density per cell";
    String long_name "Mean Cd";
    String units "femtomol/cubic micrometers (fmol um-3)";
  }
  se_cd {
    Float32 _FillValue NaN;
    Float32 actual_range 0.292, 1.118;
    String bcodmo_name "C";
    String description "Carbon density per cell standard error";
    String long_name "Se Cd";
    String units "femtomol/cubic micrometers (fmol um-3)";
  }
  mean_nd {
    Float32 _FillValue NaN;
    Float32 actual_range 0.062, 1.157;
    String bcodmo_name "N";
    String description "Mean nitrogen density per cell";
    String long_name "Mean Nd";
    String units "femtomol/cubic micrometers (fmol um-3)";
  }
  se_nd {
    Float32 _FillValue NaN;
    Float32 actual_range 0.017, 0.11;
    String bcodmo_name "N";
    String description "Nitrogen density per cell standard error";
    String long_name "Se Nd";
    String units "femtomol/cubic micrometers (fmol um-3)";
  }
 }
  NC_GLOBAL {
    String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson";
    String acquisition_description 
"Complete methods are outlined in Anderson and Rynearson, 2020, in press.
 
Elemental Analyses: From each culture and during exponential growth phase, >1
x 107 cells were filtered in triplicate onto precombusted 25-mm GF/F filters,
and rinsed with 10 ml F/2 media. Blanks were made to correct for any elemental
contribution from filters or media. Filters were dried at 60 degrees C for 24
hrs. The mass of the filter, total volume filtered, and cell counts were
utilized to determine the number of cells analyzed. Elemental composition was
assessed on an elemental analyzer.
 
Cell volume: Linear measurements of height and diameter were recorded for 30
live cells from each strain and used to calculate cell volume using the volume
of a cylinder (Montagnes and Franklin 2001).
 
All data processing was carried out in R 3.4.1(R-Core-Team 2015).";
    String awards_0_award_nid "712795";
    String awards_0_award_number "OCE-1638834";
    String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1638834";
    String awards_0_funder_name "NSF Division of Ocean Sciences";
    String awards_0_funding_acronym "NSF OCE";
    String awards_0_funding_source_nid "355";
    String awards_0_program_manager "Michael E. Sieracki";
    String awards_0_program_manager_nid "50446";
    String cdm_data_type "Other";
    String comment 
"Elemental data for Skeletonema species 
   PI's: T. Rynearson, S. Anderson (URI) 
   version date: 2019-10-30 
   Data analyzed in Anderson & Rynearson, 2019";
    String Conventions "COARDS, CF-1.6, ACDD-1.3";
    String creator_email "info@bco-dmo.org";
    String creator_name "BCO-DMO";
    String creator_type "institution";
    String creator_url "https://www.bco-dmo.org/";
    String data_source "extract_data_as_tsv version 2.3  19 Dec 2019";
    String date_created "2019-10-31T17:16:57Z";
    String date_modified "2020-02-03T19:55:19Z";
    String defaultDataQuery "&amp;time&lt;now";
    String doi "10.1575/1912/bco-dmo.780386.1";
    Float64 Easternmost_Easting -71.39;
    Float64 geospatial_lat_max 41.47;
    Float64 geospatial_lat_min 41.47;
    String geospatial_lat_units "degrees_north";
    Float64 geospatial_lon_max -71.39;
    Float64 geospatial_lon_min -71.39;
    String geospatial_lon_units "degrees_east";
    String history 
"2020-08-10T08:35:25Z (local files)
2020-08-10T08:35:25Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_780386.html";
    String infoUrl "https://www.bco-dmo.org/dataset/780386";
    String institution "BCO-DMO";
    String instruments_0_acronym "CHN_EA";
    String instruments_0_dataset_instrument_description "Used for elemental analyses.";
    String instruments_0_dataset_instrument_nid "780393";
    String instruments_0_description "A CHN Elemental Analyzer is used for the determination of carbon, hydrogen, and  nitrogen content in organic and other types of materials, including  solids, liquids, volatile, and viscous samples.";
    String instruments_0_instrument_name "CHN Elemental Analyzer";
    String instruments_0_instrument_nid "625";
    String instruments_0_supplied_name "Elemental analyzer (CE-440, Exeter Analytical, North Chelmsford, MA)";
    String instruments_1_dataset_instrument_description "Used t measure cell volume.";
    String instruments_1_dataset_instrument_nid "780394";
    String instruments_1_description "Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a \"light microscope\".";
    String instruments_1_instrument_external_identifier "https://vocab.nerc.ac.uk/collection/L05/current/LAB05/";
    String instruments_1_instrument_name "Microscope-Optical";
    String instruments_1_instrument_nid "708";
    String instruments_1_supplied_name "Eclipse E800 microscope (Nikon, Tokyo, Japan)";
    String keywords "bank, bco, bco-dmo, biological, chemical, collection, data, dataset, date, dmo, erddap, gen, GenBank, isolation, Isolation_Lat, Isolation_Lon, Isolation_Temperature, management, mean, mean_cd, mean_nd, meanC, meanCN, meanN, meanV, oceanography, office, preliminary, se_cd, se_nd, seC, seCN, seN, seV, species, strain, temperature, time, v";
    String license "https://www.bco-dmo.org/dataset/780386/license";
    String metadata_source "https://www.bco-dmo.org/api/dataset/780386";
    Float64 Northernmost_Northing 41.47;
    String param_mapping "{'780386': {'Isolation_Lon': 'flag - longitude', 'Isolation_Lat': 'flag - latitude'}}";
    String parameter_source "https://www.bco-dmo.org/mapserver/dataset/780386/parameters";
    String people_0_affiliation "University of Rhode Island";
    String people_0_affiliation_acronym "URI-GSO";
    String people_0_person_name "Tatiana Rynearson";
    String people_0_person_nid "511706";
    String people_0_role "Principal Investigator";
    String people_0_role_type "originator";
    String people_1_affiliation "University of Rhode Island";
    String people_1_affiliation_acronym "URI-GSO";
    String people_1_person_name "Stephanie Anderson";
    String people_1_person_nid "775001";
    String people_1_role "Contact";
    String people_1_role_type "related";
    String people_2_affiliation "Woods Hole Oceanographic Institution";
    String people_2_affiliation_acronym "WHOI BCO-DMO";
    String people_2_person_name "Nancy Copley";
    String people_2_person_nid "50396";
    String people_2_role "BCO-DMO Data Manager";
    String people_2_role_type "related";
    String project "Phytoplankton Community Responses";
    String projects_0_acronym "Phytoplankton Community Responses";
    String projects_0_description 
"NSF Award Abstract:
Photosynthetic marine microbes, phytoplankton, contribute half of global primary production, form the base of most aquatic food webs and are major players in global biogeochemical cycles. Understanding their community composition is important because it affects higher trophic levels, the cycling of energy and elements and is sensitive to global environmental change. This project will investigate how phytoplankton communities respond to two major global change stressors in aquatic systems: warming and changes in nutrient availability. The researchers will work in two marine systems with a long history of environmental monitoring, the temperate Narragansett Bay estuary in Rhode Island and a subtropical North Atlantic site near Bermuda. They will use field sampling and laboratory experiments with multiple species and varieties of phytoplankton to assess the diversity in their responses to different temperatures under high and low nutrient concentrations. If the diversity of responses is high within species, then that species may have a better chance to adapt to rising temperatures and persist in the future. Some species may already be able to grow at high temperatures; consequently, they may become more abundant as the ocean warms. The researchers will incorporate this response information in mathematical models to predict how phytoplankton assemblages would reorganize under future climate scenarios. Graduate students and postdoctoral associates will be trained in diverse scientific approaches and techniques such as shipboard sampling, laboratory experiments, genomic analyses and mathematical modeling. The results of the project will be incorporated into K-12 teaching, including an advanced placement environmental science class for underrepresented minorities in Los Angeles, data exercises for rural schools in Michigan and disseminated to the public through an environmental journalism institute based in Rhode Island.
Predicting how ecological communities will respond to a changing environment requires knowledge of genetic, phylogenetic and functional diversity within and across species. This project will investigate how the interaction of phylogenetic, genetic and functional diversity in thermal traits within and across a broad range of species determines the responses of marine phytoplankton communities to rising temperature and changing nutrient regimes. High genetic and functional diversity within a species may allow evolutionary adaptation of that species to warming. If the phylogenetic and functional diversity is higher across species, species sorting and ecological community reorganization is likely. Different marine sites may have a different balance of genetic and functional diversity within and across species and, thus, different contribution of evolutionary and ecological responses to changing climate. The research will be conducted at two long-term time series sites in the Atlantic Ocean, the Narragansett Bay Long-Term Plankton Time Series and the Bermuda Atlantic Time Series (BATS) station. The goal is to assess intra- and inter-specific genetic and functional diversity in thermal responses at contrasting nutrient concentrations for a representative range of species in communities at the two sites in different seasons, and use this information to parameterize eco-evolutionary models embedded into biogeochemical ocean models to predict responses of phytoplankton communities to projected rising temperatures under realistic nutrient conditions. Model predictions will be informed by and tested with field data, including the long-term data series available for both sites and in community temperature manipulation experiments. This project will provide novel information on existing intraspecific genetic and functional thermal diversity for many ecologically and biogeochemically important phytoplankton species, estimate generation of new genetic and functional diversity in evolution experiments, and develop and parameterize novel eco-evolutionary models interfaced with ocean biogeochemical models to predict future phytoplankton community structure. The project will also characterize the interaction of two major global change stressors, warming and changing nutrient concentrations, as they affect phytoplankton diversity at functional, genetic, and phylogenetic levels. In addition, the project will develop novel modeling methodology that will be broadly applicable to understanding how other types of complex ecological communities may adapt to a rapidly warming world.";
    String projects_0_end_date "2020-09";
    String projects_0_geolocation "Narragansett Bay, RI and Bermuda, Bermuda Atlantic Time-series Study (BATS)";
    String projects_0_name "Dimensions: Collaborative Research: Genetic, functional and phylogenetic diversity determines marine phytoplankton community responses to changing temperature and nutrients";
    String projects_0_project_nid "712787";
    String projects_0_start_date "2016-10";
    String publisher_name "Biological and Chemical Oceanographic Data Management Office (BCO-DMO)";
    String publisher_type "institution";
    String sourceUrl "(local files)";
    Float64 Southernmost_Northing 41.47;
    String standard_name_vocabulary "CF Standard Name Table v55";
    String summary "Elemental concentrations for Skeletonema species (n=2), as analyzed in Anderson and Rynearson, 2020. This dataset includes carbon, nitrogen, and volume measurements for S. marinoi (n=3) and S. pseudocostatum (n=4) evaluated at the thermal minimum, maximum, and optimum for each strain. Strains were isolated from Narragansett Bay, Rhode Island.";
    String title "Elemental carbon and nitrogen data for Skeletonema species as analyzed in Anderson and Rynearson, 2020";
    String version "1";
    Float64 Westernmost_Easting -71.39;
    String xml_source "osprey2erddap.update_xml() v1.3";
  }
}

 

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