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| Dataset Title: | Equivalent spherical diameter of Heterosigma akashiwo grown at different temperatures 
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| Institution: | BCO-DMO (Dataset ID: bcodmo_dataset_783509) |
| Information: | Summary
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| ISO 19115
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Attributes {
s {
Culture {
String bcodmo_name "sample";
String description "culture identifier";
String long_name "Culture";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P02/current/ACYC/";
String units "unitless";
}
Temperature {
Byte _FillValue 127;
Byte actual_range 6, 31;
String bcodmo_name "temperature";
String description "temperature treatment";
String long_name "Temperature";
String nerc_identifier "https://vocab.nerc.ac.uk/collection/P01/current/TEMPP901/";
String units "degrees Celsius";
}
MeanESD {
Float32 _FillValue NaN;
Float32 actual_range 8.6, 18.4;
String bcodmo_name "diameter";
String description "Mean Equivalent Spherical Diameter for a culture at single observations";
String long_name "Mean ESD";
String units "micrometers";
}
}
NC_GLOBAL {
String access_formats ".htmlTable,.csv,.json,.mat,.nc,.tsv";
String acquisition_description
"Experimental setup
H. akashiwo (CCMP 3374) was isolated from Narragansett Bay June 10, 2010 when
in situ water temperature was 21.2\\u00b0C. After isolation the culture was
maintained at 15\\u00b0C. All experiments were conducted with cells that were
grown in autoclaved, 0.2 \\u00b5m sterile-filtered seawater (30-31 ppt) amended
with F/2 media without silica (Guillard 1975). All cultures were maintained
under a light intensity of 150 \\u00b5mol photons m-2 s-1 and a 12:12 h light:
dark cycle. Preliminary experiments to determine the characteristics of this
strains\\u2019 growth patterns were used to maintain cultures in exponential
phase by transferring cultures as needed every 4 to 10 days (depending on
growth temperature), resulting in cell densities of 500-24,000 cell
mL-1.\\u00a0 To avoid convolution of thermal response and effect of
unconditioned media just after transfer culture transfers were restricted from
1 day prior and 1 day post change in temperature (Grabski and Tukaj 2008 ).
Temperature treatments
Temperature was manipulated in the experiment through a series of sequential
temperature shifts. Beginning with a culture which had remained at 15 \\u00b0C
since collection, every four days a triplicate set of the most extreme,
current temperature treatments were split with one fraction retained at its
current treatment and one fraction shifted a temperature step outward (i.e.
further towards the temperature extremes).\\u00a0 Only the cultures growing at
the highest and lowest temperatures were split and transferred to new
temperatures, but all other cultures were continually maintained in
exponential growth phase.\\u00a0 Cultures maintained at 15 \\u00b0C throughout
the duration of the experiment served as an acclimated control, and as a
reference for the temporal consistency in acclimated rates. Including all
temperature steps and extrema, the nine treatments included: 6, 8, 10, 12, 18,
22, 25, 28, 31, and the control at 15\\u00b0C. It took 20 days to complete the
individual temperature shifts at 4-day intervals to reach the most extreme
temperature treatments.
Dedicated incubators were used for control (15\\u00b0C, Model 2015 Low
Temperature Incubator, VWR Scientific); 4, 6 (I-41LLVL, Percival Scientific);
8, 18, 22 (I-36LLVL, Percival Scientific); and 10 \\u00b0C (Environ Air, Holman
Engineering). Twenty-five, 28, and 31 \\u00b0C treatments were accomplished
with clear 10 L baths controlled by a coupled aquarium heater and thermostat
(Fluval, Tru Temp), housed in an illuminated incubator. Light intensity was
consistently controlled across temperature treatments.
Population and growth rate measurements
To quantify changes in cell size, population growth rate, and volumetric
growth rate, the abundance and size (Equivalent Spherical Diameter, ESD)
distribution of each culture were measured with a 100 \\u00b5m aperture on a
Beckman Coulter Multisizer 3 (Beckman Coulter, Brea California; Kim and
Menden-Deuer 2013). The default bin size was the instrument standard of 0.2
\\u00b5m. Each treatment set was measured daily for 15 days following the
initial transfer to the target temperature. Experiments were terminated after
15 days because the objective of these experiments was to establish the
response to relatively short-term temperature fluctuations. \\u00a0
Statistical analysis
Mean and standard deviation of size distribution and cell counts as measured
by the Coulter Counter were estimated from the raw data by a gaussian
distribution, fit with maximum likelihood estimation to the frequency of
particles greater than 8 \\u00b5m. Measurements that immediately followed
culture inoculation and those from the growth were omitted. Cell volumes were
estimated with equivalent spherical diameter (ESD) and spherical shape
approximation.
These experiments were conducted at the University of Rhode Island, Graduate
School of Oceanography in the laboratory of Dr. Susanne Menden-Deuer";
String awards_0_award_nid "739231";
String awards_0_award_number "OCE-1736635";
String awards_0_data_url "http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1736635";
String awards_0_funder_name "NSF Division of Ocean Sciences";
String awards_0_funding_acronym "NSF OCE";
String awards_0_funding_source_nid "355";
String awards_0_program_manager "David L. Garrison";
String awards_0_program_manager_nid "50534";
String cdm_data_type "Other";
String comment
"Cell size as ESD
Equivalent spherical diameters of Heterosigma akashiwo raised at mulitple temperatures
PI: S. Menden-Deuer (URI)
version date: 2020-01-06
replaces version 2019-12-04";
String Conventions "COARDS, CF-1.6, ACDD-1.3";
String creator_email "info@bco-dmo.org";
String creator_name "BCO-DMO";
String creator_type "institution";
String creator_url "https://www.bco-dmo.org/";
String data_source "extract_data_as_tsv version 2.3 19 Dec 2019";
String date_created "2019-12-04T19:43:45Z";
String date_modified "2020-01-09T14:54:16Z";
String defaultDataQuery "&time<now";
String doi "10.1575/1912/bco-dmo.783509.2";
String history
"2024-04-18T06:07:22Z (local files)
2024-04-18T06:07:22Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_783509.html";
String infoUrl "https://www.bco-dmo.org/dataset/783509";
String institution "BCO-DMO";
String instruments_0_dataset_instrument_description "Used to count cells.";
String instruments_0_dataset_instrument_nid "783514";
String instruments_0_description
"An apparatus for counting and sizing particles suspended in electrolytes. It is used for cells, bacteria, prokaryotic cells and virus particles. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions.
from https://en.wikipedia.org/wiki/Coulter_counter";
String instruments_0_instrument_name "Coulter Counter";
String instruments_0_instrument_nid "668847";
String instruments_0_supplied_name "Beckman Coulter Multisizer III Counter";
String keywords "bco, bco-dmo, biological, chemical, culture, data, dataset, dmo, erddap, esd, management, mean, MeanESD, oceanography, office, preliminary, temperature";
String license "https://www.bco-dmo.org/dataset/783509/license";
String metadata_source "https://www.bco-dmo.org/api/dataset/783509";
String param_mapping "{'783509': {}}";
String parameter_source "https://www.bco-dmo.org/mapserver/dataset/783509/parameters";
String people_0_affiliation "University of Rhode Island";
String people_0_affiliation_acronym "URI-GSO";
String people_0_person_name "Susanne Menden-Deuer";
String people_0_person_nid "739234";
String people_0_role "Principal Investigator";
String people_0_role_type "originator";
String people_1_affiliation "Woods Hole Oceanographic Institution";
String people_1_affiliation_acronym "WHOI BCO-DMO";
String people_1_person_name "Nancy Copley";
String people_1_person_nid "50396";
String people_1_role "BCO-DMO Data Manager";
String people_1_role_type "related";
String project "Planktonic Herbivore Temp Dependence";
String projects_0_acronym "Planktonic Herbivore Temp Dependence";
String projects_0_description
"NSF Award Abstract:
Plankton, single-celled organisms that inhabit the world's oceans are responsible for the generation of oxygen, cycling energy and matter between the atmosphere and the deep ocean and are the basis for virtually all seafood harvested. These life-giving functions critically depend on the relative rates at which plankton grow and get eaten. How temperature influences those rates is essential to understand plankton responses to environmental changes and ocean dynamics. It is well established that plankton grow faster when temperatures are higher however, whether feeding has a similar temperature dependence is unknown. That means oceanographers are missing key data required to build global predictive models. This project will fill essential knowledge gaps and measure physiological rates of singled celled zooplankton across temperature gradients representing the global ocean, from polar to tropical regions and throughout the seasonal cycle. Researchers will combine laboratory experiments with specimens taken from the coastal ocean (Narragansett Bay), which is exemplary in its strong seasonal temperature variations. These data will provide a clear picture of the production capacity and activity of plankton in a global and dynamic ocean. The project supports an early career scientist, as well as graduate and undergraduate students. Scientists will continue communicating their research to the public through large-scale outreach events, education at the high-school level, and engagement through online and other media. Moreover, researchers will continue collaborating with the Metcalf Institute for Marine & Environmental Reporting to support their Annual Science Immersion Workshop for Journalists and their ongoing work to disseminate research findings through web-based seminars.
Grazing is the single largest loss factor of marine primary production and thus affects a key transfer rate between global organic and inorganic matter pools. Remarkably, data for herbivorous protist growth and grazing rates at temperatures representative of the vast polar regions and during winter and spring periods are extremely sparse. By combining laboratory experiments with ground truthing fieldwork, this project alleviates a central knowledge gap in oceanography and delivers the empirical measurements necessary to derive algorithms to incorporate temperature dependence of heterotrophic protist growth and grazing rates into biogeochemical models. The extraordinary seasonal temperature fluctuations in a temperate coastal estuary (Narragansett Bay) are exploited to measure rates of heterotrophic protists isolated from different temperatures and seasons and to quantify the temperature and acclimation responses of these ecotypes. This project delivers data urgently needed to solve the conundrum of whether herbivorous growth and predation is depressed at low temperatures, implying low trophic transfer rates and high carbon export, or if predation proceeds at rates comparable to temperate systems with primary production largely lost to predation. Large temperature gradients in the global ocean mean that cross-biome and biogeochemical models are particularly sensitive to assumptions about the temperature dependence in modeled rate processes. Establishment of the dependence of heterotrophic plankton physiological rates (growth and grazing) to gradients of temperature, mimicking realistic conditions experienced by plankton in a changing ocean, is a key step towards integrating much needed biological information in biogeochemical modeling efforts. This project makes a significant contribution to linking ecological research with ecosystem models by providing empirically rooted algorithms of the temperature dependence of protistan herbivory and growth rates, key processes in the transformation of organic matter in global biogeochemical cycles and tools critically missing in ecosystem models.";
String projects_0_end_date "2020-08";
String projects_0_geolocation "Narragansett Bay";
String projects_0_name "Quantifying Temperature Dependence In Growth & Grazing Rates of Planktonic Herbivores";
String projects_0_project_nid "739232";
String projects_0_start_date "2017-09";
String publisher_name "Biological and Chemical Oceanographic Data Management Office (BCO-DMO)";
String publisher_type "institution";
String sourceUrl "(local files)";
String standard_name_vocabulary "CF Standard Name Table v55";
String summary "A key component of the effects of temperature on growth and production of phytoplankton is not only through changes in numerical abundance, but also biomass and proxies for biomass such as biovolume. To understand how temperature affected size and biovolumetric growth, repeated measurements of the mean size feature, equivalent spherical diameter (ESD), were recorded for each experimental replicate culture of Heterosigma akashiwo across a broad range of temperature treatments. These data were collected as part of the experiment designed to study the effect of changing temperature on phytoplankton growth rates.";
String title "Equivalent spherical diameter of Heterosigma akashiwo grown at different temperatures";
String version "2";
String xml_source "osprey2erddap.update_xml() v1.3";
}
}
Data Access Protocol (DAP) and its
selection constraints.
The URL specifies what you want: the dataset, a description of the graph or the subset of the data, and the file type for the response.
Tabledap request URLs must be in the form
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/datasetID.fileType{?query}
For example,
https://coastwatch.pfeg.noaa.gov/erddap/tabledap/pmelTaoDySst.htmlTable?longitude,latitude,time,station,wmo_platform_code,T_25&time>=2015-05-23T12:00:00Z&time<=2015-05-31T12:00:00Z
Thus, the query is often a comma-separated list of desired variable names,
followed by a collection of
constraints (e.g., variable<value),
each preceded by '&' (which is interpreted as "AND").
For details, see the tabledap Documentation.