bcodmo_dataset_908514_v1 eng UTF8 dataset service Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org pointOfContact 2026-04-17 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) 2 column row [Neocalanus flemingeri oocyte production] - Oocytes formation in post-diapause Neocalanus flemingeri females from the R/V Sikuliaq and the R/V Tiglax in the Northern Gulf of Alaska from 2019-06-30 to 2019-09-13 (Collaborative Proposal: Optimizing Recruitment of Neocalanus copepods through Strategic Timing of Reproduction and Growth in the Gulf of Alaska) 2026-04-17 creation erddap.bco-dmo.org bcodmo_dataset_908514_v1 BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/908514 information web browser Background Information Background information from the source information originator These data are from a study on the formation of oocytes in post-diapause Neocalanus flemingeri females collected from depth in Prince William Sound in the Gulf of Alaska. Collections were made during two NGA LTER cruises. After sorting, females were incubated in flasks and removed for experimental incubations and imaging. Oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2'-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU labeled cells peaking at 72 hours following diapause termination. Cells labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. By limiting DNA replication to the initial phase, the females effectively separate oocyte production from oocyte provisioning. BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/908514 information web browser Background Information Background information from the source information pointOfContact earth science atmosphere ocean biosphere biology environment theme BCO-DMO project latitude longitude theme CF The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO largerWorkCitation project eng geoscientificInformation 1 -147.987 -147.803 60.278 60.535 [Neocalanus flemingeri oocyte production] - Oocytes formation in post-diapause Neocalanus flemingeri females from the R/V Sikuliaq and the R/V Tiglax in the Northern Gulf of Alaska from 2019-06-30 to 2019-09-13 (Collaborative Proposal: Optimizing Recruitment of Neocalanus copepods through Strategic Timing of Reproduction and Growth in the Gulf of Alaska) 2026-04-17 creation BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/908514 information web browser Background Information Background information from the source information originator These data are from a study on the formation of oocytes in post-diapause Neocalanus flemingeri females collected from depth in Prince William Sound in the Gulf of Alaska. Collections were made during two NGA LTER cruises. After sorting, females were incubated in flasks and removed for experimental incubations and imaging. Oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2'-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU labeled cells peaking at 72 hours following diapause termination. Cells labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. By limiting DNA replication to the initial phase, the females effectively separate oocyte production from oocyte provisioning. ERDDAP tabledap 1 -147.987 -147.803 60.278 60.535 tight ERDDAPtabledapDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908514_v1 ERDDAP:tabledap ERDDAP-tabledap ERDDAP's tabledap service (a flavor of OPeNDAP) for tabular (sequence) data. Add different extensions (e.g., .html, .graph, .das, .dds) to the base URL for different purposes. download [Neocalanus flemingeri oocyte production] - Oocytes formation in post-diapause Neocalanus flemingeri females from the R/V Sikuliaq and the R/V Tiglax in the Northern Gulf of Alaska from 2019-06-30 to 2019-09-13 (Collaborative Proposal: Optimizing Recruitment of Neocalanus copepods through Strategic Timing of Reproduction and Growth in the Gulf of Alaska) 2026-04-17 creation BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/908514 information web browser Background Information Background information from the source information originator These data are from a study on the formation of oocytes in post-diapause Neocalanus flemingeri females collected from depth in Prince William Sound in the Gulf of Alaska. Collections were made during two NGA LTER cruises. After sorting, females were incubated in flasks and removed for experimental incubations and imaging. Oocyte production by post-diapause females that involved DNA replication in the ovary and oviducts was examined using incubation in 5-Ethynyl-2'-deoxyuridine (EdU). Both oogonia and oocytes incorporated EdU, with the number of EdU labeled cells peaking at 72 hours following diapause termination. Cells labeling with EdU remained high for two weeks, decreasing thereafter with no labeling detected by four weeks post diapause, and three to four weeks before spawning of the first clutch of eggs. By limiting DNA replication to the initial phase, the females effectively separate oocyte production from oocyte provisioning. OPeNDAP 1 -147.987 -147.803 60.278 60.535 tight OPeNDAPDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908514_v1 OPeNDAP:OPeNDAP OPeNDAP An OPeNDAP service for tabular (sequence) data. Add different extensions (e.g., .html, .das, .dds) to the base URL for different purposes. download physicalMeasurement Station String Station Date_collected String Date_collected Collection_month String Collection_month Species String Species Sex String Sex EdU_sample_ID_number String Edu_sample_id_number Amount_of_EdU float Amount_of_edu Incubation_date_at_start String Incubation_date_at_start Length_of_EdU_incubation_in_hours int Length_of_edu_incubation_in_hours Time_point_in_days float Time_point_in_days Number_of_cells_replicating int Number_of_cells_replicating Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org distributor OPeNDAP DAP/2.0 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908514_v1.html order Data Subset Form ERDDAP's version of the OPeNDAP .html web page for this dataset. Specify a subset of the dataset and download the data via OPeNDAP or in many different file types. download https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_908514_v1.graph order Make-A-Graph Form ERDDAP's Make-A-Graph .html web page for this dataset. Create an image with a map or graph of a subset of the data. mapDigital This record was created from dataset metadata by ERDDAP Version 2.22