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Dataset Title: | [Synechococcus Growth on DOP Experiments - IVF] - Laboratory-cultured Synechococcus (WH8102 and WH5701) growth (vivo fluorescence) on dissolved organic phosphorus (DOP) from experiments between 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle) |
Institution: | BCO-DMO (Dataset ID: bcodmo_dataset_929212_v1) |
Information: | Summary | License | Metadata | Background | Files | Make a graph |
Attributes { s { synechococcus_strain { String long_name "Synechococcus_strain"; String units "unitless"; } experiment_number { Int32 actual_range 1, 2; String long_name "Experiment_number"; String units "unitless"; } time_day { Int32 actual_range 0, 14; String long_name "Time_day"; String units "days"; } DOP_substrate { String long_name "Dop_substrate"; String units "unitless"; } in_vivo_fluorescence_trip1 { Int32 actual_range 14, 387; String long_name "In_vivo_fluorescence_trip1"; String units "relative fluorescence units (RFU)"; } in_vivo_fluorescence_trip2 { Int32 actual_range 10, 403; String long_name "In_vivo_fluorescence_trip2"; String units "relative fluorescence units (RFU)"; } in_vivo_fluorescence_trip3 { Int32 actual_range 13, 461; String long_name "In_vivo_fluorescence_trip3"; String units "relative fluorescence units (RFU)"; } } NC_GLOBAL { String cdm_data_type "Other"; String Conventions "COARDS, CF-1.6, ACDD-1.3"; String creator_email "info@bco-dmo.org"; String creator_name "BCO-DMO"; String creator_url "https://www.bco-dmo.org/"; String doi "10.26008/1912/bco-dmo.929212.1"; String history "2024-11-13T21:34:31Z (local files) 2024-11-13T21:34:31Z https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_929212_v1.html"; String infoUrl "https://www.bco-dmo.org/dataset/929212"; String institution "BCO-DMO"; String license "The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information."; String sourceUrl "(local files)"; String summary "Laboratory culture Synechococcus (WH8102 and WH5701) growth on dissolved organic phosphorus (DOP). These data were collected as part of a study of \"Dissolved organic Phosphorus bond-class utilization by Synechococcus\" (Waggoner et al. 2024). Study Abstract: Dissolved organic phosphorus (DOP) contains compounds with phosphoester (P-O-C), phosphoanhydride (P-O-P), and phosphorus-carbon (P-C) bonds. Despite DOP's importance as a nutritional source for marine microorganisms, the bioavailability of each bond-class to the widespread cyanobacterium Synechococcus remains largely unknown. This study evaluates bond-class specific DOP utilization by cultures of an open ocean and a coastal ocean Synechococcus strain. Both strains exhibited comparable growth rates when provided phosphate, short-chain and long-chain polyphosphate (P-O-P), adenosine 5'-triphosphate (P-O-C and P-O-P), and glucose-6-phosphate (P-O-C) as the phosphorus source. However, growth rates on phosphomonoester adenosine 5'-monophosphate (P-O-C) and phosphodiester bis(4-methylumbelliferyl) phosphate (C-O-P-O-C) varied between strains, and neither strain grew on selected phosphonates. Consistent with the growth measurements, both strains preferentially hydrolyzed 3-polyphosphate, followed by adenosine 5'-triphosphate, and then adenosine 5'-monophosphate. The strains' exoproteome contained phosphorus hydrolases, which combined with enhanced cell-free hydrolysis of 3-polyphosphate and adenosine 5'-triphosphate under phosphate deficiency, suggests active mineralization of short-chain polyphosphate by Synechococcus' exoproteins. Synechococcus alkaline phosphatases presented broad substrate specificities, including activity towards short-chain polyphosphate, with varying affinities between the two strains. Collectively, these findings underscore the potentially significant role of compounds with phosphoanhydride bonds in Synechococcus phosphorus nutrition, thereby expanding our understanding of microbially-mediated DOP cycling in marine ecosystems."; String title "[Synechococcus Growth on DOP Experiments - IVF] - Laboratory-cultured Synechococcus (WH8102 and WH5701) growth (vivo fluorescence) on dissolved organic phosphorus (DOP) from experiments between 2018-2023 (Collaborative Research: Assessing the role of compound-specific phosphorus hydrolase transformations in the marine phosphorus cycle)"; } }
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