bcodmo_dataset_936224_v1 eng UTF8 dataset service Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org pointOfContact 2026-04-24 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) 4 column row vertical temporal [Virus production at a hydrothermal vent site] - Virus and prokaryote abundances from experiments conducted with samples collected at a hydrothermal vent site by ROV SuBastian during R/V Falkor (too) expedition FKt230627 along the East Pacific Rise in July of 2023 (The Underworld of Hydrothermal Vents) 2026-04-24 creation erddap.bco-dmo.org bcodmo_dataset_936224_v1 BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/936224 information web browser Background Information Background information from the source information originator We used the virus-dilution technique to quantify lytic virus production at nine sites across vent, sub-vent, and non-vent habitats along the East Pacific Rise during expedition FKt230627 aboard R/V Falkor(too). This technique leverages the density-dependent nature of viral infection, preventing new infections by dilution. Samples were collected via ROV SuBastian, sequentially filtered to remove larger particles, and concentrated using tangential-flow filtration. Duplicate incubations were set up with prokaryotic concentrate and virus-free water, pressurized to 250 bar, and incubated in the dark at in situ temperatures for 30 hours. Subsamples were taken every six hours for enumeration of prokaryotes and viruses via flow cytometry. Parallel experiments were conducted at surface pressure to assess the impact of pressure on virus production rates. The methodology ensures observed increases in viral abundance are due to pre-existing infections. The experiments were conducted aboard R/V Falkor(too) between 5-22 July 2023 by Tinkara Tinta and Nicole Krause. Prokaryotes and viruses were enumerated flow-cytometrically back in the lab by Christian Winter. BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/936224 information web browser Background Information Background information from the source information pointOfContact earth science atmosphere ocean biosphere biology environment theme BCO-DMO project latitude longitude depth time theme CF The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO largerWorkCitation project eng geoscientificInformation 1 -104.2919 -104.2916 9.839953 9.840335 seconds 2023-07-06T00:45:00Z 2023-07-23T04:25:00Z -2518.14 -2505.64 [Virus production at a hydrothermal vent site] - Virus and prokaryote abundances from experiments conducted with samples collected at a hydrothermal vent site by ROV SuBastian during R/V Falkor (too) expedition FKt230627 along the East Pacific Rise in July of 2023 (The Underworld of Hydrothermal Vents) 2026-04-24 creation BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/936224 information web browser Background Information Background information from the source information originator We used the virus-dilution technique to quantify lytic virus production at nine sites across vent, sub-vent, and non-vent habitats along the East Pacific Rise during expedition FKt230627 aboard R/V Falkor(too). This technique leverages the density-dependent nature of viral infection, preventing new infections by dilution. Samples were collected via ROV SuBastian, sequentially filtered to remove larger particles, and concentrated using tangential-flow filtration. Duplicate incubations were set up with prokaryotic concentrate and virus-free water, pressurized to 250 bar, and incubated in the dark at in situ temperatures for 30 hours. Subsamples were taken every six hours for enumeration of prokaryotes and viruses via flow cytometry. Parallel experiments were conducted at surface pressure to assess the impact of pressure on virus production rates. The methodology ensures observed increases in viral abundance are due to pre-existing infections. The experiments were conducted aboard R/V Falkor(too) between 5-22 July 2023 by Tinkara Tinta and Nicole Krause. Prokaryotes and viruses were enumerated flow-cytometrically back in the lab by Christian Winter. ERDDAP tabledap 1 -104.2919 -104.2916 9.839953 9.840335 seconds 2023-07-06T00:45:00Z 2023-07-23T04:25:00Z -2518.14 -2505.64 tight ERDDAPtabledapDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_936224_v1 ERDDAP:tabledap ERDDAP-tabledap ERDDAP's tabledap service (a flavor of OPeNDAP) for tabular (sequence) data. Add different extensions (e.g., .html, .graph, .das, .dds) to the base URL for different purposes. download [Virus production at a hydrothermal vent site] - Virus and prokaryote abundances from experiments conducted with samples collected at a hydrothermal vent site by ROV SuBastian during R/V Falkor (too) expedition FKt230627 along the East Pacific Rise in July of 2023 (The Underworld of Hydrothermal Vents) 2026-04-24 creation BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/936224 information web browser Background Information Background information from the source information originator We used the virus-dilution technique to quantify lytic virus production at nine sites across vent, sub-vent, and non-vent habitats along the East Pacific Rise during expedition FKt230627 aboard R/V Falkor(too). This technique leverages the density-dependent nature of viral infection, preventing new infections by dilution. Samples were collected via ROV SuBastian, sequentially filtered to remove larger particles, and concentrated using tangential-flow filtration. Duplicate incubations were set up with prokaryotic concentrate and virus-free water, pressurized to 250 bar, and incubated in the dark at in situ temperatures for 30 hours. Subsamples were taken every six hours for enumeration of prokaryotes and viruses via flow cytometry. Parallel experiments were conducted at surface pressure to assess the impact of pressure on virus production rates. The methodology ensures observed increases in viral abundance are due to pre-existing infections. The experiments were conducted aboard R/V Falkor(too) between 5-22 July 2023 by Tinkara Tinta and Nicole Krause. Prokaryotes and viruses were enumerated flow-cytometrically back in the lab by Christian Winter. OPeNDAP 1 -104.2919 -104.2916 9.839953 9.840335 seconds 2023-07-06T00:45:00Z 2023-07-23T04:25:00Z -2518.14 -2505.64 tight OPeNDAPDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_936224_v1 OPeNDAP:OPeNDAP OPeNDAP An OPeNDAP service for tabular (sequence) data. Add different extensions (e.g., .html, .das, .dds) to the base URL for different purposes. download physicalMeasurement dive String Dive sample_id String Sample_id subsample_id String Subsample_id habitat String Habitat temperature float Temperature incubated_at_250bar String Incubated_at_250bar date_local String Date_local time_local String Time_local time double Iso_datetime_utc prokaryotes float Prokaryotes viruses float Viruses Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org distributor OPeNDAP DAP/2.0 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_936224_v1.html order Data Subset Form ERDDAP's version of the OPeNDAP .html web page for this dataset. Specify a subset of the dataset and download the data via OPeNDAP or in many different file types. download https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_936224_v1.graph order Make-A-Graph Form ERDDAP's Make-A-Graph .html web page for this dataset. Create an image with a map or graph of a subset of the data. mapDigital This record was created from dataset metadata by ERDDAP Version 2.22