bcodmo_dataset_953999_v1 eng UTF8 dataset service Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org pointOfContact 2026-04-16 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) 2 column row [16S microbiome data for artificial reef sponges and seawater] - 16S microbiome metadata collected from shallow artificial reef sponges and seawater in the Florida Keys, USA from Apr 2021 to Aug 2021 (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) 2026-04-16 creation erddap.bco-dmo.org bcodmo_dataset_953999_v1 BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/953999 information web browser Background Information Background information from the source information originator Sponges are a dominant component of coral reefs worldwide and in the Caribbean, where their biomass exceeds that of reef-building corals. For almost a quarter century, the success of sponges in the Caribbean has been linked to their filter-feeding ability. However, recent work demonstrated that coexisting sponges on Caribbean reefs host unique communities of bacteria that might allow sponges to access multiple pools of nutrients that are not available to other organisms. In this project, the investigators will test the hypothesis that ecologically dominant sponge species in the Caribbean have unique metabolic strategies that are mediated by their associations with microbes that live within the sponge body. In this dataset, we present the 16S rRNA microbiome NCBI accession and sample collection metadata for an artificial reef experiment where sponges of 10 species were placed on this temporary reef from April to August of 2021 and sampled using VacuSIP. VacuSIP methods capture incurrent (In) and excurrent (Ex) water from each sponge specimen. Incurrent represents the bacteria that are available for the sponge to consume via filter feeding and excurrent represents the bacteria that remain once sponges have consumed their preferred taxa. Additionally, we have provided microbiome metadata for the host sponges for several of these paired In/Ex samples. See the related dataset, NCBI Bioproject PRJNA1179970, for all sequence data. Microbiome data was generated using protocols from the Earth Microbiome project and sequencing was conducted on an Illumina MiSeq at Middle Tennessee State University. The data available at NCBI represents raw sequencing data, and no quality checks or sequencing filtering has been done on the uploaded sequences. BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/953999 information web browser Background Information Background information from the source information pointOfContact earth science atmosphere ocean biosphere biology environment theme BCO-DMO project latitude longitude theme CF The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO largerWorkCitation project eng geoscientificInformation 1 -81.39122 -81.39122 24.56779 24.56779 [16S microbiome data for artificial reef sponges and seawater] - 16S microbiome metadata collected from shallow artificial reef sponges and seawater in the Florida Keys, USA from Apr 2021 to Aug 2021 (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) 2026-04-16 creation BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/953999 information web browser Background Information Background information from the source information originator Sponges are a dominant component of coral reefs worldwide and in the Caribbean, where their biomass exceeds that of reef-building corals. For almost a quarter century, the success of sponges in the Caribbean has been linked to their filter-feeding ability. However, recent work demonstrated that coexisting sponges on Caribbean reefs host unique communities of bacteria that might allow sponges to access multiple pools of nutrients that are not available to other organisms. In this project, the investigators will test the hypothesis that ecologically dominant sponge species in the Caribbean have unique metabolic strategies that are mediated by their associations with microbes that live within the sponge body. In this dataset, we present the 16S rRNA microbiome NCBI accession and sample collection metadata for an artificial reef experiment where sponges of 10 species were placed on this temporary reef from April to August of 2021 and sampled using VacuSIP. VacuSIP methods capture incurrent (In) and excurrent (Ex) water from each sponge specimen. Incurrent represents the bacteria that are available for the sponge to consume via filter feeding and excurrent represents the bacteria that remain once sponges have consumed their preferred taxa. Additionally, we have provided microbiome metadata for the host sponges for several of these paired In/Ex samples. See the related dataset, NCBI Bioproject PRJNA1179970, for all sequence data. Microbiome data was generated using protocols from the Earth Microbiome project and sequencing was conducted on an Illumina MiSeq at Middle Tennessee State University. The data available at NCBI represents raw sequencing data, and no quality checks or sequencing filtering has been done on the uploaded sequences. ERDDAP tabledap 1 -81.39122 -81.39122 24.56779 24.56779 tight ERDDAPtabledapDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_953999_v1 ERDDAP:tabledap ERDDAP-tabledap ERDDAP's tabledap service (a flavor of OPeNDAP) for tabular (sequence) data. Add different extensions (e.g., .html, .graph, .das, .dds) to the base URL for different purposes. download [16S microbiome data for artificial reef sponges and seawater] - 16S microbiome metadata collected from shallow artificial reef sponges and seawater in the Florida Keys, USA from Apr 2021 to Aug 2021 (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) 2026-04-16 creation BCO-DMO info@bco-dmo.org https://www.bco-dmo.org/dataset/953999 information web browser Background Information Background information from the source information originator Sponges are a dominant component of coral reefs worldwide and in the Caribbean, where their biomass exceeds that of reef-building corals. For almost a quarter century, the success of sponges in the Caribbean has been linked to their filter-feeding ability. However, recent work demonstrated that coexisting sponges on Caribbean reefs host unique communities of bacteria that might allow sponges to access multiple pools of nutrients that are not available to other organisms. In this project, the investigators will test the hypothesis that ecologically dominant sponge species in the Caribbean have unique metabolic strategies that are mediated by their associations with microbes that live within the sponge body. In this dataset, we present the 16S rRNA microbiome NCBI accession and sample collection metadata for an artificial reef experiment where sponges of 10 species were placed on this temporary reef from April to August of 2021 and sampled using VacuSIP. VacuSIP methods capture incurrent (In) and excurrent (Ex) water from each sponge specimen. Incurrent represents the bacteria that are available for the sponge to consume via filter feeding and excurrent represents the bacteria that remain once sponges have consumed their preferred taxa. Additionally, we have provided microbiome metadata for the host sponges for several of these paired In/Ex samples. See the related dataset, NCBI Bioproject PRJNA1179970, for all sequence data. Microbiome data was generated using protocols from the Earth Microbiome project and sequencing was conducted on an Illumina MiSeq at Middle Tennessee State University. The data available at NCBI represents raw sequencing data, and no quality checks or sequencing filtering has been done on the uploaded sequences. OPeNDAP 1 -81.39122 -81.39122 24.56779 24.56779 tight OPeNDAPDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_953999_v1 OPeNDAP:OPeNDAP OPeNDAP An OPeNDAP service for tabular (sequence) data. Add different extensions (e.g., .html, .das, .dds) to the base URL for different purposes. download physicalMeasurement collection_date String Collection_date accession String Accession sample_name String Sample_name species_ab String Species_ab sample_type String Sample_type organism String Organism AphiaID String Aphiaid LSID String Lsid isolation_source String Isolation_source geo_loc_name String Geo_loc_name data_day int Data_day seq_attempt String Seq_attempt Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org distributor OPeNDAP DAP/2.0 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_953999_v1.html order Data Subset Form ERDDAP's version of the OPeNDAP .html web page for this dataset. Specify a subset of the dataset and download the data via OPeNDAP or in many different file types. download https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_953999_v1.graph order Make-A-Graph Form ERDDAP's Make-A-Graph .html web page for this dataset. Create an image with a map or graph of a subset of the data. mapDigital This record was created from dataset metadata by ERDDAP Version 2.22