bcodmo_dataset_954735_v1 eng UTF8 dataset service Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org pointOfContact 2026-04-16 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) 2 column row [Autotrophy, heterotrophy, and niche partitioning in Caribbean sponges] - Autotrophy, heterotrophy and niche partitioning in Caribbean sponges sampled June 9, 2019 on reef sites around Bocas del Toro Panama. (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) 2026-04-16 creation erddap.bco-dmo.org bcodmo_dataset_954735_v1 BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/954735 information web browser Background Information Background information from the source information originator Photosymbionts expand the metabolic capabilities of host sponges, but their potential role in mediating niche partitioning on crowded and oligotrophic coral reefs is unknown. To address this question, we conducted two ex situ isotope tracer experiments with ten of the most ecologically dominant sponge species in the Caribbean. This research was carried out in Bocas del Toro, Panama. To target autotrophic and heterotrophic nutrient acquisition by microbial symbionts, we incubated sponges in seawater laced with the inorganic compounds NaH13CO3 and Na15NO3 under both light and dark conditions. We also measured host sponge heterotrophic feeding rates by incubating the same species with 13C- and 15N-labeled bacterial cells. Sponge cells isolated from sponge species hosting photosymbionts were significantly more enriched in 13C and 15N from inorganic sources, and 72 % of the variation in 13C and 15N enrichment across samples was explained by sponge species identity. Dark enrichment of 13C was minimal, but all species were enriched in 15N in the dark due to heterotrophic microbial nitrogen assimilation. Sponges rapidly consumed bacterial cells, but there was substantial variation in heterotrophic feeding rates among sponge species. When considering all three resource pools (symbiont autotrophy, symbiont heterotrophy, and sponge heterotrophy) and both elements, sponge species identity accounted for over 80 % of variation among specimens; in addition, we observed a clear separation of sponge species along a continuum of heterotrophic feeding on particulate organic matter to autotrophic metabolism via photosymbionts. These data demonstrate that the combined influence of sponge and photosymbiont metabolism enable coexisting sponge species to exploit unique resource pools on Caribbean reefs. BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/954735 information web browser Background Information Background information from the source information pointOfContact earth science atmosphere ocean biosphere biology environment theme BCO-DMO project latitude longitude theme CF The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO largerWorkCitation project eng geoscientificInformation 1 -82.26245 -82.26245 9.349457 9.349457 [Autotrophy, heterotrophy, and niche partitioning in Caribbean sponges] - Autotrophy, heterotrophy and niche partitioning in Caribbean sponges sampled June 9, 2019 on reef sites around Bocas del Toro Panama. (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) 2026-04-16 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/954735 information web browser Background Information Background information from the source information originator Photosymbionts expand the metabolic capabilities of host sponges, but their potential role in mediating niche partitioning on crowded and oligotrophic coral reefs is unknown. To address this question, we conducted two ex situ isotope tracer experiments with ten of the most ecologically dominant sponge species in the Caribbean. This research was carried out in Bocas del Toro, Panama. To target autotrophic and heterotrophic nutrient acquisition by microbial symbionts, we incubated sponges in seawater laced with the inorganic compounds NaH13CO3 and Na15NO3 under both light and dark conditions. We also measured host sponge heterotrophic feeding rates by incubating the same species with 13C- and 15N-labeled bacterial cells. Sponge cells isolated from sponge species hosting photosymbionts were significantly more enriched in 13C and 15N from inorganic sources, and 72 % of the variation in 13C and 15N enrichment across samples was explained by sponge species identity. Dark enrichment of 13C was minimal, but all species were enriched in 15N in the dark due to heterotrophic microbial nitrogen assimilation. Sponges rapidly consumed bacterial cells, but there was substantial variation in heterotrophic feeding rates among sponge species. When considering all three resource pools (symbiont autotrophy, symbiont heterotrophy, and sponge heterotrophy) and both elements, sponge species identity accounted for over 80 % of variation among specimens; in addition, we observed a clear separation of sponge species along a continuum of heterotrophic feeding on particulate organic matter to autotrophic metabolism via photosymbionts. These data demonstrate that the combined influence of sponge and photosymbiont metabolism enable coexisting sponge species to exploit unique resource pools on Caribbean reefs. ERDDAP tabledap 1 -82.26245 -82.26245 9.349457 9.349457 tight ERDDAPtabledapDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_954735_v1 ERDDAP:tabledap ERDDAP-tabledap ERDDAP's tabledap service (a flavor of OPeNDAP) for tabular (sequence) data. Add different extensions (e.g., .html, .graph, .das, .dds) to the base URL for different purposes. download [Autotrophy, heterotrophy, and niche partitioning in Caribbean sponges] - Autotrophy, heterotrophy and niche partitioning in Caribbean sponges sampled June 9, 2019 on reef sites around Bocas del Toro Panama. (Collaborative Research: Investigations into microbially mediated ecological diversification in sponges) 2026-04-16 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/954735 information web browser Background Information Background information from the source information originator Photosymbionts expand the metabolic capabilities of host sponges, but their potential role in mediating niche partitioning on crowded and oligotrophic coral reefs is unknown. To address this question, we conducted two ex situ isotope tracer experiments with ten of the most ecologically dominant sponge species in the Caribbean. This research was carried out in Bocas del Toro, Panama. To target autotrophic and heterotrophic nutrient acquisition by microbial symbionts, we incubated sponges in seawater laced with the inorganic compounds NaH13CO3 and Na15NO3 under both light and dark conditions. We also measured host sponge heterotrophic feeding rates by incubating the same species with 13C- and 15N-labeled bacterial cells. Sponge cells isolated from sponge species hosting photosymbionts were significantly more enriched in 13C and 15N from inorganic sources, and 72 % of the variation in 13C and 15N enrichment across samples was explained by sponge species identity. Dark enrichment of 13C was minimal, but all species were enriched in 15N in the dark due to heterotrophic microbial nitrogen assimilation. Sponges rapidly consumed bacterial cells, but there was substantial variation in heterotrophic feeding rates among sponge species. When considering all three resource pools (symbiont autotrophy, symbiont heterotrophy, and sponge heterotrophy) and both elements, sponge species identity accounted for over 80 % of variation among specimens; in addition, we observed a clear separation of sponge species along a continuum of heterotrophic feeding on particulate organic matter to autotrophic metabolism via photosymbionts. These data demonstrate that the combined influence of sponge and photosymbiont metabolism enable coexisting sponge species to exploit unique resource pools on Caribbean reefs. OPeNDAP 1 -82.26245 -82.26245 9.349457 9.349457 tight OPeNDAPDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_954735_v1 OPeNDAP:OPeNDAP OPeNDAP An OPeNDAP service for tabular (sequence) data. Add different extensions (e.g., .html, .das, .dds) to the base URL for different purposes. download physicalMeasurement Species String Species Full_scientific_name String Full_scientific_name Date String Date Replicate int Replicate Fraction String Fraction Initial_d15N float Initial_d15n Initial_wt_percN float Initial_wt_percn Initial_Atm_perc15N float Initial_atm_perc15n Initial_d13C float Initial_d13c Initial_wt_percC float Initial_wt_percc Atm_perc13C float Atm_perc13c Dark_d15N float Dark_d15n Dark_wt_percN float Dark_wt_percn Dark_Atm_perc15N float Dark_atm_perc15n Dark_d13C float Dark_d13c Dark_wt_percC float Dark_wt_percc Dark_Atm_perc13C float Dark_atm_perc13c Dark_Change_in_d15N float Dark_change_in_d15n Dark_Change_in_d13C float Dark_change_in_d13c Light_d15N float Light_d15n Light_wt_percN float Light_wt_percn Light_Atm_perc15N float Light_atm_perc15n Light_d13C float Light_d13c Light_wt_percC float Light_wt_percc Light_Atm_perc13C float Light_atm_perc13c Light_Change_in_d15N float Light_change_in_d15n Light_Change_in_d13C float Light_change_in_d13c Light_photo_d15N float Light_photo_d15n light_photo_d13C float Light_photo_d13c Hetero_d15N float Hetero_d15n hetero_d13C float Hetero_d13c POM_heterotrophy_enriched_d13C float Pom_heterotrophy_enriched_d13c POM_heterotrophy_enriched_d15N float Pom_heterotrophy_enriched_d15n Heterotrophy_POM_change_in_d13c float Heterotrophy_pom_change_in_d13c Heterotrophy_POM_change_in_d15N float Heterotrophy_pom_change_in_d15n POM_T60_CR float Pom_t60_cr POM_T120_CR float Pom_t120_cr Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org distributor OPeNDAP DAP/2.0 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_954735_v1.html order Data Subset Form ERDDAP's version of the OPeNDAP .html web page for this dataset. Specify a subset of the dataset and download the data via OPeNDAP or in many different file types. download https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_954735_v1.graph order Make-A-Graph Form ERDDAP's Make-A-Graph .html web page for this dataset. Create an image with a map or graph of a subset of the data. mapDigital This record was created from dataset metadata by ERDDAP Version 2.22