[AE2413 FlaPS bulk] - Polysaccharide hydrolase activities from water samples collected at various sites under varying hydrostatic pressures in the Western North Atlantic aboard R/V Atlantic Explorer cruise AE2413 in May 2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean)

Project: BCO-DMO Name: BCO-DMO Email: info@bco-dmo.org Institution: BCO-DMO InfoURL: https://osprey.bco-dmo.org/dataset/968956 Source URL: (local files) [AE2413 FlaPS bulk] - Polysaccharide hydrolase activities from water samples collected at various sites under varying hydrostatic pressures in the Western North Atlantic aboard R/V Atlantic Explorer cruise AE2413 in May 2024 (Collaborative Research: Pressure effects on microbially-catalyzed organic matter degradation in the deep ocean) Unknown tabular digital data Woods Hole, MA, USA ERDDAP, version 2.22, at BCO-DMO https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph BCO-DMO Heterotrophic bacteria and archaea (here: microbes) are critical drivers of the ocean's biogeochemical cycles, active throughout the depth of the ocean. Their capabilities and limitations help determine the rates and locations at which carbon and nutrients are regenerated, as well as the extent to which organic matter is preserved (Hedges 1992). In the deep ocean, at bathy- and abyssopelagic depths (ca. 1000-6000m), these communities are dependent upon the sinking flux of particulate organic matter (POM) from the surface ocean (Bergauer et al. 2018). This dependence means that heterotrophic microbial communities must produce the extracellular enzymes required to solubilize and hydrolyze high molecular weight (HMW) POM to sizes substrates suitable for cellular uptake. A recent global-scale investigation of deep-sea microbes in fact found that the genetic potential for exported (extracellular) enzymes among bacteria in deep waters was far greater than for communities in surface or mesopelagic waters (Zhao et al. 2020). We have new evidence that a substantial fraction of bacteria in bottom water from the North Atlantic Ocean use a specialized set of extracellular enzymes to rapidly take up HMW polysaccharides (Giljan et al. 2021), a substrate processing mechanism that would not be detected with the low molecular weight substrates used in most prior studies of microbial activity in the deep ocean (Nagata et al. 2010). Through our collaboration with the Danish Center for Hadal Research, we were able to use pressurization systems and in situ specialized equipment to investigate the effects of pressures characteristic of bathy- and abyssopelagic depths on microbial communities and their extracellular enzymes in the open North Atlantic Ocean. Here we present the measurement of polysaccharide hydrolase activities above that measured for killed controls from various sites under varying hydrostatic pressures in the Western North Atlantic aboard R/V Atlantic Explorer, cruise AE2413 in May 2024. Unknown https://osprey.bco-dmo.org/dataset/968956 20240510 20240519 ground condition Complete As needed -73.03333 -60.0479 42.17828 34.99333 Uncontrolled earth science atmosphere ocean biosphere biology environment Uncontrolled latitude longitude time depth None. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO BCO-DMO Unknown Mailing and Physical Address

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Unknown Unknown Unknown Unknown Unknown info@bco-dmo.org https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph Web page to make a customized map or graph of the data HTML Unknown Unknown Unknown Unknown Unknown Unknown Point Point BCO-DMO Adam Shepherd Technical Director Mailing and Physical Address

266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org ERDDAP, version 2.22: get metadata; download data; make graphs and maps. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. .asc 1 View OPeNDAP-style ISO-8859-1 comma-separated text. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#ASCII_Service https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .csv 1 Download a ISO-8859-1 comma-separated text table (line 1: names; line 2: units; ISO 8601 times). https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .csvp 1 Download a ISO-8859-1 .csv file with line 1: name (units). 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Times are ISO 8601 strings. https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .dataTable 1 A JSON file formatted for use with the Google Visualization client library (Google Charts). https://developers.google.com/chart/interactive/docs/reference#dataparam https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .das 1 View the dataset's metadata via an ISO-8859-1 OPeNDAP Dataset Attribute Structure (DAS). https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Dataset_Attribute_Structure https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .dds 1 View the dataset's structure via an ISO-8859-1 OPeNDAP Dataset Descriptor Structure (DDS). https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Dataset_Descriptor_Structure https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .dods 1 OPeNDAP clients use this to download the data in the DODS binary format. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Data_Transmission https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .esriCsv 1 Download a ISO_8859_1 .csv file for ESRI's ArcGIS 9.x and below (separate date and time columns). https://support.esri.com/technical-article/000012745 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .fgdc 1 View the dataset's UTF-8 FGDC .xml metadata. https://www.fgdc.gov/standards/projects/FGDC-standards-projects/metadata/base-metadata/index_html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .geoJson 1 Download longitude,latitude,otherColumns data as a UTF-8 GeoJSON .json file. http://wiki.geojson.org/Main_Page https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .graph 1 View a Make A Graph web page. https://coastwatch.pfeg.noaa.gov/erddap/tabledap/documentation.html#GraphicsCommands https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .help 1 View a web page with a description of tabledap. https://www.opendap.org/pdf/ESE-RFC-004v1.2.pdf https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .html 1 View an OPeNDAP-style HTML Data Access Form. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#WWW_Interface_Service https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .htmlTable 1 View a UTF-8 .html web page with the data in a table. Times are ISO 8601 strings. https://www.w3schools.com/html/html_tables.asp https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .iso19115 1 View the dataset's ISO 19115-2/19139 UTF-8 .xml metadata. https://en.wikipedia.org/wiki/Geospatial_metadata https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .itx 1 Download an ISO-8859-1 Igor Text File. Each response column becomes a wave. https://www.wavemetrics.net/doc/igorman/II-09%20Data%20Import%20Export.pdf https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .json 1 View a table-like UTF-8 JSON file (missing value = 'null'; times are ISO 8601 strings). https://www.json.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .jsonlCSV1 1 View a UTF-8 JSON Lines CSV file with column names on line 1 (mv = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .jsonlCSV 1 View a UTF-8 JSON Lines CSV file without column names (mv = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .jsonlKVP 1 View a UTF-8 JSON Lines file with Key:Value pairs (missing value = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .mat 1 Download a MATLAB binary file. https://www.mathworks.com/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .nc 1 Download a flat, table-like, NetCDF-3 binary file with COARDS/CF/ACDD metadata. https://www.unidata.ucar.edu/software/netcdf/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .ncHeader 1 View the UTF-8 header (the metadata) for the NetCDF-3 .nc file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .ncCF 1 Download a NetCDF-3 CF Discrete Sampling Geometries file (Contiguous Ragged Array). https://cfconventions.org/Data/cf-conventions/cf-conventions-1.8/cf-conventions.html#discrete-sampling-geometries https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .ncCFHeader 1 View the UTF-8 header (the metadata) for the .ncCF file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .ncCFMA 1 Download a NetCDF-3 CF Discrete Sampling Geometries file (Multidimensional Array). https://www.ncei.noaa.gov/netcdf-templates https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .ncCFMAHeader 1 View the UTF-8 header (the metadata) for the .ncCFMA file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .nccsv 1 Download a NetCDF-3-like 7-bit ASCII NCCSV .csv file with COARDS/CF/ACDD metadata. https://coastwatch.pfeg.noaa.gov/erddap/download/NCCSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .nccsvMetadata 1 View the dataset's metadata as the top half of a 7-bit ASCII NCCSV .csv file. https://coastwatch.pfeg.noaa.gov/erddap/download/NCCSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .ncoJson 1 Download a UTF-8 NCO lvl=2 JSON file with COARDS/CF/ACDD metadata. https://nco.sourceforge.net/nco.html#json https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .odvTxt 1 Download longitude,latitude,time,otherColumns as an ISO-8859-1 ODV Generic Spreadsheet File (.txt). https://odv.awi.de/en/documentation/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .subset 1 View an HTML form which uses faceted search to simplify picking subsets of the data. https://en.wikipedia.org/wiki/Faceted_search https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .tsv 1 Download a ISO-8859-1 tab-separated text table (line 1: names; line 2: units; ISO 8601 times). https://jkorpela.fi/TSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .tsvp 1 Download a ISO-8859-1 .tsv file with line 1: name (units). 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Times are ISO 8601 strings. https://www.w3schools.com/html/html_tables.asp https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.html .kml 1 View a .kml file, suitable for Google Earth. https://developers.google.com/kml/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph .smallPdf 1 View a small .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph .pdf 1 View a standard, medium-sized .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph .largePdf 1 View a large .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph .smallPng 1 View a small .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph .png 1 View a standard, medium-sized .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph .largePng 1 View a large .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph .transparentPng 1 View a .png image file (just the data, without axes, landmask, or legend). http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_968956_v1.graph None 20260811 BCO-DMO Adam Shepherd Technical Director Mailing and Physical Address

266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org Content Standard for Digital Geospatial Metadata FGDC-STD-012-2002 universal time None. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information.