bcodmo_dataset_986605_v1 eng UTF8 dataset service Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org pointOfContact 2026-08-24 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) 3 column row vertical [OC1704A metagenome accession numbers] - Metagenome accession numbers for samples collected in the eastern tropical North Pacific Ocean in 2017 on R/V Oceanus cruise 1704A (CAREER: Oxygen sensitivity of aerobic respiration and nitrification in oxygen minimum zones and biogeochemical feedbacks to deoxygenation) 2026-08-24 creation erddap.bco-dmo.org bcodmo_dataset_986605_v1 BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/986605 information web browser Background Information Background information from the source information originator Water samples were collected for DNA extraction using sampling bottles deployed on the CTD rosette. At each depth, 2L samples were filtered through 0.22 µm filters (Millipore, Darmstadt, Germany) using a peristaltic pump and frozen until extraction. DNA was extracted following Beman et al. (2012) and sent for metagenome sequencing in the Vincent J. Coates Genome Sequencing Laboratory (GSL) at the University of California, Berkeley. For each sample, 250 ng of genomic DNA was sheared and libraries were prepared using the KAPA HyperPrep Kit (Kapa Biosystems, Wilmington, MA, USA). Samples were pooled into a single lane and sequenced via 150- cycle paired-end sequencing on the Illumina HiSeq 4000 platform (Illumina, Inc., San Diego, CA, USA). BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/986605 information web browser Background Information Background information from the source information pointOfContact earth science atmosphere ocean biosphere biology environment theme BCO-DMO project latitude longitude depth theme CF The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO largerWorkCitation project eng geoscientificInformation 1 -110.0 -106.5 16.0 20.5 -180.0 -25.0 [OC1704A metagenome accession numbers] - Metagenome accession numbers for samples collected in the eastern tropical North Pacific Ocean in 2017 on R/V Oceanus cruise 1704A (CAREER: Oxygen sensitivity of aerobic respiration and nitrification in oxygen minimum zones and biogeochemical feedbacks to deoxygenation) 2026-08-24 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/986605 information web browser Background Information Background information from the source information originator Water samples were collected for DNA extraction using sampling bottles deployed on the CTD rosette. At each depth, 2L samples were filtered through 0.22 µm filters (Millipore, Darmstadt, Germany) using a peristaltic pump and frozen until extraction. DNA was extracted following Beman et al. (2012) and sent for metagenome sequencing in the Vincent J. Coates Genome Sequencing Laboratory (GSL) at the University of California, Berkeley. For each sample, 250 ng of genomic DNA was sheared and libraries were prepared using the KAPA HyperPrep Kit (Kapa Biosystems, Wilmington, MA, USA). Samples were pooled into a single lane and sequenced via 150- cycle paired-end sequencing on the Illumina HiSeq 4000 platform (Illumina, Inc., San Diego, CA, USA). ERDDAP tabledap 1 -110.0 -106.5 16.0 20.5 -180.0 -25.0 tight ERDDAPtabledapDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986605_v1 ERDDAP:tabledap ERDDAP-tabledap ERDDAP's tabledap service (a flavor of OPeNDAP) for tabular (sequence) data. Add different extensions (e.g., .html, .graph, .das, .dds) to the base URL for different purposes. download [OC1704A metagenome accession numbers] - Metagenome accession numbers for samples collected in the eastern tropical North Pacific Ocean in 2017 on R/V Oceanus cruise 1704A (CAREER: Oxygen sensitivity of aerobic respiration and nitrification in oxygen minimum zones and biogeochemical feedbacks to deoxygenation) 2026-08-24 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/986605 information web browser Background Information Background information from the source information originator Water samples were collected for DNA extraction using sampling bottles deployed on the CTD rosette. At each depth, 2L samples were filtered through 0.22 µm filters (Millipore, Darmstadt, Germany) using a peristaltic pump and frozen until extraction. DNA was extracted following Beman et al. (2012) and sent for metagenome sequencing in the Vincent J. Coates Genome Sequencing Laboratory (GSL) at the University of California, Berkeley. For each sample, 250 ng of genomic DNA was sheared and libraries were prepared using the KAPA HyperPrep Kit (Kapa Biosystems, Wilmington, MA, USA). Samples were pooled into a single lane and sequenced via 150- cycle paired-end sequencing on the Illumina HiSeq 4000 platform (Illumina, Inc., San Diego, CA, USA). OPeNDAP 1 -110.0 -106.5 16.0 20.5 -180.0 -25.0 tight OPeNDAPDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986605_v1 OPeNDAP:OPeNDAP OPeNDAP An OPeNDAP service for tabular (sequence) data. Add different extensions (e.g., .html, .das, .dds) to the base URL for different purposes. download physicalMeasurement Date String Date Station int Station Repository String Repository Biosample_accession String Biosample_accession Sequencing_technology String Sequencing_technology Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org distributor OPeNDAP DAP/2.0 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986605_v1.html order Data Subset Form ERDDAP's version of the OPeNDAP .html web page for this dataset. Specify a subset of the dataset and download the data via OPeNDAP or in many different file types. download https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986605_v1.graph order Make-A-Graph Form ERDDAP's Make-A-Graph .html web page for this dataset. Create an image with a map or graph of a subset of the data. mapDigital This record was created from dataset metadata by ERDDAP Version 2.22