[Water column methane, methane oxidation, and pmoA gene copies] - Water column methane, methane oxidation, and pmoA gene copies above southern California methane seeps determined from samples collected on R/V Atlantis cruise AT50-12 in July 2023 (Collaborative Research: Redefining the footprint of deep ocean methane seepage for benthic ecosystems)

Project: BCO-DMO Name: BCO-DMO Email: info@bco-dmo.org Institution: BCO-DMO InfoURL: https://osprey.bco-dmo.org/dataset/986866 Source URL: (local files) [Water column methane, methane oxidation, and pmoA gene copies] - Water column methane, methane oxidation, and pmoA gene copies above southern California methane seeps determined from samples collected on R/V Atlantis cruise AT50-12 in July 2023 (Collaborative Research: Redefining the footprint of deep ocean methane seepage for benthic ecosystems) Unknown tabular digital data Woods Hole, MA, USA ERDDAP, version 2.22, at BCO-DMO https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.graph BCO-DMO Marine methane (CH₄) seeps are dynamic biogeochemical systems that modulate carbon cycling and support high-biomass communities through microbial methane (CH₄) oxidation. While most CH₄ is consumed anaerobically in sediments, a fraction enters the water column, where aerobic methanotrophs form a biological filter limiting CH₄ flux to the atmosphere. However, the extent to which this microbial activity and CH₄ influence extend beyond visibly active seep zones remains poorly constrained, with implications for deep-sea food webs, biogeochemical gradients, and carbon cycling. We investigated CH₄ dynamics and methanotroph distribution across three seep sites on the Southern California margin (Del Mar, Santa Monica (800 meters) Mound, Lasuen Knoll; ~400–1200 meters depth). Using radiotracer (³H-CH₄) incubations, CH₄ concentration profiling, and particulate methane monooxygenase (pmoA) gene quantification, we sampled vertical and horizontal transects and near-bottom waters via the HOV Alvin. CH₄ oxidation was active not only within seep plumes but also in off-seep regions, with the highest rate (454 nanomoles per liter per day) observed within a CH₄-rich bubble plume. pmoA gene abundances remained relatively stable across both seep and off-seep waters, suggesting a persistent CH₄-oxidizing potential. These findings support an expanded "methanosphere," a CH₄-influenced microbial zone shaped by physical transport and environmental gradients. This dataset is part of a PhD thesis: Klonicki-Ference, Emily F. "Microbial Regulation of Methane and Redox Dynamics in the Water Column: From a Proterozoic Ocean Analog to Modern Marine Seeps." PhD diss., University of California, Los Angeles, 2025. Unknown https://osprey.bco-dmo.org/dataset/986866 20230717 20230726 ground condition Complete As needed -118.6488 -117.7765 33.8003 32.8339 Uncontrolled earth science atmosphere ocean biosphere biology environment Uncontrolled time latitude longitude None. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO BCO-DMO Unknown Mailing and Physical Address

Unknown

Unknown Unknown Unknown Unknown Unknown info@bco-dmo.org https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.graph Web page to make a customized map or graph of the data HTML Unknown Unknown Unknown Unknown Unknown Unknown Point Point BCO-DMO Adam Shepherd Technical Director Mailing and Physical Address

266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org ERDDAP, version 2.22: get metadata; download data; make graphs and maps. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. .asc 1 View OPeNDAP-style ISO-8859-1 comma-separated text. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#ASCII_Service https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .csv 1 Download a ISO-8859-1 comma-separated text table (line 1: names; line 2: units; ISO 8601 times). https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .csvp 1 Download a ISO-8859-1 .csv file with line 1: name (units). Times are ISO 8601 strings. https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .csv0 1 Download a ISO-8859-1 .csv file without column names or units. Times are ISO 8601 strings. https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .dataTable 1 A JSON file formatted for use with the Google Visualization client library (Google Charts). https://developers.google.com/chart/interactive/docs/reference#dataparam https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .das 1 View the dataset's metadata via an ISO-8859-1 OPeNDAP Dataset Attribute Structure (DAS). https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Dataset_Attribute_Structure https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .dds 1 View the dataset's structure via an ISO-8859-1 OPeNDAP Dataset Descriptor Structure (DDS). https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Dataset_Descriptor_Structure https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .dods 1 OPeNDAP clients use this to download the data in the DODS binary format. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Data_Transmission https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .esriCsv 1 Download a ISO_8859_1 .csv file for ESRI's ArcGIS 9.x and below (separate date and time columns). https://support.esri.com/technical-article/000012745 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .fgdc 1 View the dataset's UTF-8 FGDC .xml metadata. https://www.fgdc.gov/standards/projects/FGDC-standards-projects/metadata/base-metadata/index_html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .geoJson 1 Download longitude,latitude,otherColumns data as a UTF-8 GeoJSON .json file. http://wiki.geojson.org/Main_Page https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .graph 1 View a Make A Graph web page. https://coastwatch.pfeg.noaa.gov/erddap/tabledap/documentation.html#GraphicsCommands https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .help 1 View a web page with a description of tabledap. https://www.opendap.org/pdf/ESE-RFC-004v1.2.pdf https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .html 1 View an OPeNDAP-style HTML Data Access Form. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#WWW_Interface_Service https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .htmlTable 1 View a UTF-8 .html web page with the data in a table. Times are ISO 8601 strings. https://www.w3schools.com/html/html_tables.asp https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .iso19115 1 View the dataset's ISO 19115-2/19139 UTF-8 .xml metadata. https://en.wikipedia.org/wiki/Geospatial_metadata https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .itx 1 Download an ISO-8859-1 Igor Text File. Each response column becomes a wave. https://www.wavemetrics.net/doc/igorman/II-09%20Data%20Import%20Export.pdf https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .json 1 View a table-like UTF-8 JSON file (missing value = 'null'; times are ISO 8601 strings). https://www.json.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .jsonlCSV1 1 View a UTF-8 JSON Lines CSV file with column names on line 1 (mv = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .jsonlCSV 1 View a UTF-8 JSON Lines CSV file without column names (mv = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .jsonlKVP 1 View a UTF-8 JSON Lines file with Key:Value pairs (missing value = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .mat 1 Download a MATLAB binary file. https://www.mathworks.com/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .nc 1 Download a flat, table-like, NetCDF-3 binary file with COARDS/CF/ACDD metadata. https://www.unidata.ucar.edu/software/netcdf/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .ncHeader 1 View the UTF-8 header (the metadata) for the NetCDF-3 .nc file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .ncCF 1 Download a NetCDF-3 CF Discrete Sampling Geometries file (Contiguous Ragged Array). https://cfconventions.org/Data/cf-conventions/cf-conventions-1.8/cf-conventions.html#discrete-sampling-geometries https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .ncCFHeader 1 View the UTF-8 header (the metadata) for the .ncCF file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .ncCFMA 1 Download a NetCDF-3 CF Discrete Sampling Geometries file (Multidimensional Array). https://www.ncei.noaa.gov/netcdf-templates https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .ncCFMAHeader 1 View the UTF-8 header (the metadata) for the .ncCFMA file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .nccsv 1 Download a NetCDF-3-like 7-bit ASCII NCCSV .csv file with COARDS/CF/ACDD metadata. https://coastwatch.pfeg.noaa.gov/erddap/download/NCCSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .nccsvMetadata 1 View the dataset's metadata as the top half of a 7-bit ASCII NCCSV .csv file. https://coastwatch.pfeg.noaa.gov/erddap/download/NCCSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .ncoJson 1 Download a UTF-8 NCO lvl=2 JSON file with COARDS/CF/ACDD metadata. https://nco.sourceforge.net/nco.html#json https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .odvTxt 1 Download longitude,latitude,time,otherColumns as an ISO-8859-1 ODV Generic Spreadsheet File (.txt). https://odv.awi.de/en/documentation/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .subset 1 View an HTML form which uses faceted search to simplify picking subsets of the data. https://en.wikipedia.org/wiki/Faceted_search https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .tsv 1 Download a ISO-8859-1 tab-separated text table (line 1: names; line 2: units; ISO 8601 times). https://jkorpela.fi/TSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .tsvp 1 Download a ISO-8859-1 .tsv file with line 1: name (units). Times are ISO 8601 strings. https://jkorpela.fi/TSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .tsv0 1 Download a ISO-8859-1 .tsv file without column names or units. Times are ISO 8601 strings. https://jkorpela.fi/TSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .wav 1 Download a .wav audio file. All columns must be numeric and of the same type. https://en.wikipedia.org/wiki/WAV https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .xhtml 1 View a UTF-8 XHTML (XML) file with the data in a table. Times are ISO 8601 strings. https://www.w3schools.com/html/html_tables.asp https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.html .kml 1 View a .kml file, suitable for Google Earth. https://developers.google.com/kml/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.graph .smallPdf 1 View a small .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.graph .pdf 1 View a standard, medium-sized .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.graph .largePdf 1 View a large .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.graph .smallPng 1 View a small .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.graph .png 1 View a standard, medium-sized .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.graph .largePng 1 View a large .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.graph .transparentPng 1 View a .png image file (just the data, without axes, landmask, or legend). http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_986866_v1.graph None 20260408 BCO-DMO Adam Shepherd Technical Director Mailing and Physical Address

266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org Content Standard for Digital Geospatial Metadata FGDC-STD-012-2002 universal time None. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information.