bcodmo_dataset_988179_v1 eng UTF8 dataset service Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org pointOfContact 2026-10-05 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) 4 column row vertical temporal [EN638 cell count selfish] - Detection and Quantification of Cells Exhibiting 'Selfish' Uptake in samples taken during R/V Endeavor cruise EN638 in the Western North Atlantic in May 2019 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation) 2026-10-05 creation erddap.bco-dmo.org bcodmo_dataset_988179_v1 BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/988179 information web browser Background Information Background information from the source information originator Marine dissolved organic carbon (DOC) is one of the largest actively-cycling carbon reservoirs on earth—comparable in magnitude to atmospheric CO2 (Hansell 2013)—and thus is an essential component of the global carbon cycle. DOC has a multitude of sources, including phytoplankton productivity, grazing, excretion, solubilization from particulate organic carbon (POC), viral lysis, and riverine input; the major DOC sink in the ocean is consumption by heterotrophic microbial communities (Carlson & Hansell 2015). Measurement of bulk DOC characteristics such as 14C age and molecular size have demonstrated that the high molecular weight (HMW) DOC fraction is generally younger and more biologically reactive than the low molecular weight (LMW) fraction (e.g. Guo et al. 1996; Walker et al. 2016). We know that a substantial fraction of HMW DOC consists of carbohydrates, including neutral sugars, and that its concentration is lower in the deep ocean than in the upper mesopelagic/surface ocean (Benner & Amon 2015). Characterization of the HMW DOC fraction has primarily used chemical measurements that provide information about monomeric constituents (Benner et al. 1992; Kaiser & Benner 2009), but yield no information on the order in which such constituents are linked together, or about the 3D structure of the intact HMW DOC. Beyond these observations and measurements, however, the specific factors controlling the rate, location, and extent to which DOC is transformed and remineralized by heterotrophic microbial communities in the ocean are still not well understood.   A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). To better contextualize this hydrolysis, bulk water characterization of the deep chlorophyll maximum (DCM), oxygen minimum zone (OMZ), and bottom waters used in our mesocosm incubation experiments was performed.   Here we present, in collaboration with colleagues from the Max Planck Institute for Marine Microbiology, the detection and quantification of microbial cells exhibiting selfish uptake behavior of fluorescently-labeled HMW polysaccharides. This dataset includes sample collection metadata, environmental variables, experimental variables, the number of cells detected exhibiting "selfish" uptake, and total cellular abundance. BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/988179 information web browser Background Information Background information from the source information pointOfContact earth science atmosphere ocean biosphere biology environment theme BCO-DMO project latitude longitude time depth theme CF The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO largerWorkCitation project eng geoscientificInformation 1 -72.0021 -53.3949 34.6369 42.83954 seconds 2019-05-17T00:20:00Z 2019-05-25T07:30:00Z -5580.0 -2.5 [EN638 cell count selfish] - Detection and Quantification of Cells Exhibiting 'Selfish' Uptake in samples taken during R/V Endeavor cruise EN638 in the Western North Atlantic in May 2019 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation) 2026-10-05 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/988179 information web browser Background Information Background information from the source information originator Marine dissolved organic carbon (DOC) is one of the largest actively-cycling carbon reservoirs on earth—comparable in magnitude to atmospheric CO2 (Hansell 2013)—and thus is an essential component of the global carbon cycle. DOC has a multitude of sources, including phytoplankton productivity, grazing, excretion, solubilization from particulate organic carbon (POC), viral lysis, and riverine input; the major DOC sink in the ocean is consumption by heterotrophic microbial communities (Carlson & Hansell 2015). Measurement of bulk DOC characteristics such as 14C age and molecular size have demonstrated that the high molecular weight (HMW) DOC fraction is generally younger and more biologically reactive than the low molecular weight (LMW) fraction (e.g. Guo et al. 1996; Walker et al. 2016). We know that a substantial fraction of HMW DOC consists of carbohydrates, including neutral sugars, and that its concentration is lower in the deep ocean than in the upper mesopelagic/surface ocean (Benner & Amon 2015). Characterization of the HMW DOC fraction has primarily used chemical measurements that provide information about monomeric constituents (Benner et al. 1992; Kaiser & Benner 2009), but yield no information on the order in which such constituents are linked together, or about the 3D structure of the intact HMW DOC. Beyond these observations and measurements, however, the specific factors controlling the rate, location, and extent to which DOC is transformed and remineralized by heterotrophic microbial communities in the ocean are still not well understood.   A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). To better contextualize this hydrolysis, bulk water characterization of the deep chlorophyll maximum (DCM), oxygen minimum zone (OMZ), and bottom waters used in our mesocosm incubation experiments was performed.   Here we present, in collaboration with colleagues from the Max Planck Institute for Marine Microbiology, the detection and quantification of microbial cells exhibiting selfish uptake behavior of fluorescently-labeled HMW polysaccharides. This dataset includes sample collection metadata, environmental variables, experimental variables, the number of cells detected exhibiting "selfish" uptake, and total cellular abundance. ERDDAP tabledap 1 -72.0021 -53.3949 34.6369 42.83954 seconds 2019-05-17T00:20:00Z 2019-05-25T07:30:00Z -5580.0 -2.5 tight ERDDAPtabledapDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988179_v1 ERDDAP:tabledap ERDDAP-tabledap ERDDAP's tabledap service (a flavor of OPeNDAP) for tabular (sequence) data. Add different extensions (e.g., .html, .graph, .das, .dds) to the base URL for different purposes. download [EN638 cell count selfish] - Detection and Quantification of Cells Exhibiting 'Selfish' Uptake in samples taken during R/V Endeavor cruise EN638 in the Western North Atlantic in May 2019 (A mechanistic microbial underpinning for the size-reactivity continuum of dissolved organic carbon degradation) 2026-10-05 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/988179 information web browser Background Information Background information from the source information originator Marine dissolved organic carbon (DOC) is one of the largest actively-cycling carbon reservoirs on earth—comparable in magnitude to atmospheric CO2 (Hansell 2013)—and thus is an essential component of the global carbon cycle. DOC has a multitude of sources, including phytoplankton productivity, grazing, excretion, solubilization from particulate organic carbon (POC), viral lysis, and riverine input; the major DOC sink in the ocean is consumption by heterotrophic microbial communities (Carlson & Hansell 2015). Measurement of bulk DOC characteristics such as 14C age and molecular size have demonstrated that the high molecular weight (HMW) DOC fraction is generally younger and more biologically reactive than the low molecular weight (LMW) fraction (e.g. Guo et al. 1996; Walker et al. 2016). We know that a substantial fraction of HMW DOC consists of carbohydrates, including neutral sugars, and that its concentration is lower in the deep ocean than in the upper mesopelagic/surface ocean (Benner & Amon 2015). Characterization of the HMW DOC fraction has primarily used chemical measurements that provide information about monomeric constituents (Benner et al. 1992; Kaiser & Benner 2009), but yield no information on the order in which such constituents are linked together, or about the 3D structure of the intact HMW DOC. Beyond these observations and measurements, however, the specific factors controlling the rate, location, and extent to which DOC is transformed and remineralized by heterotrophic microbial communities in the ocean are still not well understood.   A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). To better contextualize this hydrolysis, bulk water characterization of the deep chlorophyll maximum (DCM), oxygen minimum zone (OMZ), and bottom waters used in our mesocosm incubation experiments was performed.   Here we present, in collaboration with colleagues from the Max Planck Institute for Marine Microbiology, the detection and quantification of microbial cells exhibiting selfish uptake behavior of fluorescently-labeled HMW polysaccharides. This dataset includes sample collection metadata, environmental variables, experimental variables, the number of cells detected exhibiting "selfish" uptake, and total cellular abundance. OPeNDAP 1 -72.0021 -53.3949 34.6369 42.83954 seconds 2019-05-17T00:20:00Z 2019-05-25T07:30:00Z -5580.0 -2.5 tight OPeNDAPDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988179_v1 OPeNDAP:OPeNDAP OPeNDAP An OPeNDAP service for tabular (sequence) data. Add different extensions (e.g., .html, .das, .dds) to the base URL for different purposes. download physicalMeasurement deployment String Deployment station String Station time double Iso_datetime_utc cast_number String Cast_number depth_name String Depth_name sample_type String Sample_type unamended_amended String Unamended_amended substrate String Substrate timepoint_days float Timepoint_days timepoint_hours float Timepoint_hours replicate_1_selfish_percent float Replicate_1_selfish_percent replicate_2_selfish_percent float Replicate_2_selfish_percent replicate_3_selfish_percent float Replicate_3_selfish_percent average_selfish_percent float Average_selfish_percent standard_deviation_selfish_percent float Standard_deviation_selfish_percent replicate_1_substratecells float Replicate_1_substratecells replicate_2_substratecells float Replicate_2_substratecells replicate_3_substratecells float Replicate_3_substratecells average_substratecells float Average_substratecells standard_deviation_substratecells float Standard_deviation_substratecells replicate_1_cells float Replicate_1_cells replicate_2_cells float Replicate_2_cells replicate_3_cells float Replicate_3_cells average_cells float Average_cells standard_deviation_cells float Standard_deviation_cells Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org distributor OPeNDAP DAP/2.0 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988179_v1.html order Data Subset Form ERDDAP's version of the OPeNDAP .html web page for this dataset. Specify a subset of the dataset and download the data via OPeNDAP or in many different file types. download https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988179_v1.graph order Make-A-Graph Form ERDDAP's Make-A-Graph .html web page for this dataset. Create an image with a map or graph of a subset of the data. mapDigital This record was created from dataset metadata by ERDDAP Version 2.22