Project: BCO-DMO Name: BCO-DMO Email: info@bco-dmo.org Institution: BCO-DMO InfoURL: https://osprey.bco-dmo.org/dataset/988643 Source URL: (local files) Unknown tabular digital data Woods Hole, MA, USA ERDDAP, version 2.22, at BCO-DMO https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph BCO-DMO A key focus of this project's field work is investigating the potential of marine heterotrophic microbial communities from different water masses and under differing conditions of organic matter availability to hydrolyze six well-characterized high-molecular-weight (HMW) polysaccharides (arabinogalactan, chondroitin sulfate, fucoidan, laminarin, pullulan, and xylan). However, the current method is extremely work-intensive, and results are usually acquired months after initial sampling. Having a proxy to determine real-time enzymatic activity would be useful for scientists doing field work because it would provide a guide as to when and where optimal conditions are located to conduct water column sampling. In an effort to improve field detection techniques, a novel and relatively rapid detection method was developed to detect the hydrolysis of fluorescently-labeled laminarin that is linked to epoxy-activated-agarose beads. Laminarin was used because it is commonly found throughout the surface water of the ocean (Becker et al. 2020). Our goal is to detect potential enzymatic activity within 48 hours using minimal lab equipment while maintaining an accuracy similar to the current standard method used in previous research. Furthermore, we hope the method will provide real-time information for scientists in the field.    This dataset includes the detection of polysaccharide enzymatic hydrolysis using a novel and relatively rapid detection method from waters taken aboard the R/V Endeavor in the Western North Atlantic during the research cruise EN683 in May and June, 2022, and from a local freshwater lake. Unknown https://osprey.bco-dmo.org/dataset/988643 Unknown Unknown ground condition Complete As needed -79.0935 -53.8678 42.8701 33.74342 Uncontrolled earth science atmosphere ocean biosphere biology environment Uncontrolled latitude longitude depth None. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO BCO-DMO Unknown Mailing and Physical Address

Unknown

Unknown Unknown Unknown Unknown Unknown info@bco-dmo.org https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph Web page to make a customized map or graph of the data HTML Unknown Unknown Unknown Unknown Unknown Unknown Point Point BCO-DMO Adam Shepherd Technical Director Mailing and Physical Address

266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org ERDDAP, version 2.22: get metadata; download data; make graphs and maps. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. .asc 1 View OPeNDAP-style ISO-8859-1 comma-separated text. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#ASCII_Service https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .csv 1 Download a ISO-8859-1 comma-separated text table (line 1: names; line 2: units; ISO 8601 times). https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .csvp 1 Download a ISO-8859-1 .csv file with line 1: name (units). Times are ISO 8601 strings. https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .csv0 1 Download a ISO-8859-1 .csv file without column names or units. Times are ISO 8601 strings. https://en.wikipedia.org/wiki/Comma-separated_values https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .dataTable 1 A JSON file formatted for use with the Google Visualization client library (Google Charts). https://developers.google.com/chart/interactive/docs/reference#dataparam https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .das 1 View the dataset's metadata via an ISO-8859-1 OPeNDAP Dataset Attribute Structure (DAS). https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Dataset_Attribute_Structure https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .dds 1 View the dataset's structure via an ISO-8859-1 OPeNDAP Dataset Descriptor Structure (DDS). https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Dataset_Descriptor_Structure https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .dods 1 OPeNDAP clients use this to download the data in the DODS binary format. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#Data_Transmission https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .esriCsv 1 Download a ISO_8859_1 .csv file for ESRI's ArcGIS 9.x and below (separate date and time columns). https://support.esri.com/technical-article/000012745 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .fgdc 1 View the dataset's UTF-8 FGDC .xml metadata. https://www.fgdc.gov/standards/projects/FGDC-standards-projects/metadata/base-metadata/index_html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .geoJson 1 Download longitude,latitude,otherColumns data as a UTF-8 GeoJSON .json file. http://wiki.geojson.org/Main_Page https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .graph 1 View a Make A Graph web page. https://coastwatch.pfeg.noaa.gov/erddap/tabledap/documentation.html#GraphicsCommands https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .help 1 View a web page with a description of tabledap. https://www.opendap.org/pdf/ESE-RFC-004v1.2.pdf https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .html 1 View an OPeNDAP-style HTML Data Access Form. https://docs.opendap.org/index.php/UserGuideOPeNDAPMessages#WWW_Interface_Service https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .htmlTable 1 View a UTF-8 .html web page with the data in a table. Times are ISO 8601 strings. https://www.w3schools.com/html/html_tables.asp https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .iso19115 1 View the dataset's ISO 19115-2/19139 UTF-8 .xml metadata. https://en.wikipedia.org/wiki/Geospatial_metadata https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .itx 1 Download an ISO-8859-1 Igor Text File. Each response column becomes a wave. https://www.wavemetrics.net/doc/igorman/II-09%20Data%20Import%20Export.pdf https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .json 1 View a table-like UTF-8 JSON file (missing value = 'null'; times are ISO 8601 strings). https://www.json.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .jsonlCSV1 1 View a UTF-8 JSON Lines CSV file with column names on line 1 (mv = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .jsonlCSV 1 View a UTF-8 JSON Lines CSV file without column names (mv = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .jsonlKVP 1 View a UTF-8 JSON Lines file with Key:Value pairs (missing value = 'null'; times are ISO 8601 strings). https://jsonlines.org/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .mat 1 Download a MATLAB binary file. https://www.mathworks.com/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .nc 1 Download a flat, table-like, NetCDF-3 binary file with COARDS/CF/ACDD metadata. https://www.unidata.ucar.edu/software/netcdf/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .ncHeader 1 View the UTF-8 header (the metadata) for the NetCDF-3 .nc file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .ncCF 1 Download a NetCDF-3 CF Discrete Sampling Geometries file (Contiguous Ragged Array). https://cfconventions.org/Data/cf-conventions/cf-conventions-1.8/cf-conventions.html#discrete-sampling-geometries https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .ncCFHeader 1 View the UTF-8 header (the metadata) for the .ncCF file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .ncCFMA 1 Download a NetCDF-3 CF Discrete Sampling Geometries file (Multidimensional Array). https://www.ncei.noaa.gov/netcdf-templates https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .ncCFMAHeader 1 View the UTF-8 header (the metadata) for the .ncCFMA file. https://linux.die.net/man/1/ncdump https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .nccsv 1 Download a NetCDF-3-like 7-bit ASCII NCCSV .csv file with COARDS/CF/ACDD metadata. https://coastwatch.pfeg.noaa.gov/erddap/download/NCCSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .nccsvMetadata 1 View the dataset's metadata as the top half of a 7-bit ASCII NCCSV .csv file. https://coastwatch.pfeg.noaa.gov/erddap/download/NCCSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .ncoJson 1 Download a UTF-8 NCO lvl=2 JSON file with COARDS/CF/ACDD metadata. https://nco.sourceforge.net/nco.html#json https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .odvTxt 1 Download longitude,latitude,time,otherColumns as an ISO-8859-1 ODV Generic Spreadsheet File (.txt). https://odv.awi.de/en/documentation/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .subset 1 View an HTML form which uses faceted search to simplify picking subsets of the data. https://en.wikipedia.org/wiki/Faceted_search https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .tsv 1 Download a ISO-8859-1 tab-separated text table (line 1: names; line 2: units; ISO 8601 times). https://jkorpela.fi/TSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .tsvp 1 Download a ISO-8859-1 .tsv file with line 1: name (units). Times are ISO 8601 strings. https://jkorpela.fi/TSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .tsv0 1 Download a ISO-8859-1 .tsv file without column names or units. Times are ISO 8601 strings. https://jkorpela.fi/TSV.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .wav 1 Download a .wav audio file. All columns must be numeric and of the same type. https://en.wikipedia.org/wiki/WAV https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .xhtml 1 View a UTF-8 XHTML (XML) file with the data in a table. Times are ISO 8601 strings. https://www.w3schools.com/html/html_tables.asp https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.html .kml 1 View a .kml file, suitable for Google Earth. https://developers.google.com/kml/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph .smallPdf 1 View a small .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph .pdf 1 View a standard, medium-sized .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph .largePdf 1 View a large .pdf image file with a graph or map. https://www.adobe.com/acrobat/about-adobe-pdf.html https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph .smallPng 1 View a small .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph .png 1 View a standard, medium-sized .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph .largePng 1 View a large .png image file with a graph or map. http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph .transparentPng 1 View a .png image file (just the data, without axes, landmask, or legend). http://www.libpng.org/pub/png/ https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_988643_v1.graph None 20260902 BCO-DMO Adam Shepherd Technical Director Mailing and Physical Address

266 Woods Hole Rd. MS#36

Woods Hole MA 02543 USA 508-289-2772 erddap@bco-dmo.org Content Standard for Digital Geospatial Metadata FGDC-STD-012-2002 universal time None. The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information.