bcodmo_dataset_997806_v1 eng UTF8 dataset service Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org pointOfContact 2026-07-24 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) 4 column row vertical temporal [Depth profiles of quantitative PCR of ammonia-oxidizing archaea and bacteria KM1314] - Depth profiles of quantitative PCR of ammonia-oxidizing archaea and bacteria from R/V Kilo Moana KM1314 in the North Pacific Ocean (Significance of nitrification in shaping planktonic biodiversity in the ocean) 2026-07-24 creation erddap.bco-dmo.org bcodmo_dataset_997806_v1 BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/997806 information web browser Background Information Background information from the source information originator This dataset reports depth-resolved abundances of archaeal and β-proteobacterial ammonia monooxygenase (amoA) genes collected at Stations 5, 8, and 13 in the northeastern Pacific Ocean during the R/V Kilo Moana cruise KM1314 in August 2013. One liter of seawater from each depth was filtered onto 0.22 μm Sterivex filters and stored at −80 °C prior to DNA extraction using a modified manual extraction protocol. Archaeal and bacterial amoA gene abundances were quantified by quantitative PCR using primer sets CrenAmoAQ-F/CrenAmoAModR and AmoA1-F/AmoA2-R on a LightCycler platform. Standard curves, amplification procedures, melting curve analyses, and gel electrophoresis verification followed established protocols. These measurements characterize vertical distributions of ammonia-oxidizing microorganisms in the upper 300 m of the water column and support analyses of nitrification activity and microbial community structure in the oligotrophic North Pacific. BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/997806 information web browser Background Information Background information from the source information pointOfContact earth science atmosphere ocean biosphere biology environment theme BCO-DMO project time latitude longitude depth theme CF The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO largerWorkCitation project eng geoscientificInformation 1 -150.0 -134.6803 30.0 50.0 seconds 2013-08-14T13:05:19Z 2013-08-29T14:50:20Z -300.0 -0.0 [Depth profiles of quantitative PCR of ammonia-oxidizing archaea and bacteria KM1314] - Depth profiles of quantitative PCR of ammonia-oxidizing archaea and bacteria from R/V Kilo Moana KM1314 in the North Pacific Ocean (Significance of nitrification in shaping planktonic biodiversity in the ocean) 2026-07-24 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/997806 information web browser Background Information Background information from the source information originator This dataset reports depth-resolved abundances of archaeal and β-proteobacterial ammonia monooxygenase (amoA) genes collected at Stations 5, 8, and 13 in the northeastern Pacific Ocean during the R/V Kilo Moana cruise KM1314 in August 2013. One liter of seawater from each depth was filtered onto 0.22 μm Sterivex filters and stored at −80 °C prior to DNA extraction using a modified manual extraction protocol. Archaeal and bacterial amoA gene abundances were quantified by quantitative PCR using primer sets CrenAmoAQ-F/CrenAmoAModR and AmoA1-F/AmoA2-R on a LightCycler platform. Standard curves, amplification procedures, melting curve analyses, and gel electrophoresis verification followed established protocols. These measurements characterize vertical distributions of ammonia-oxidizing microorganisms in the upper 300 m of the water column and support analyses of nitrification activity and microbial community structure in the oligotrophic North Pacific. ERDDAP tabledap 1 -150.0 -134.6803 30.0 50.0 seconds 2013-08-14T13:05:19Z 2013-08-29T14:50:20Z -300.0 -0.0 tight ERDDAPtabledapDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_997806_v1 ERDDAP:tabledap ERDDAP-tabledap ERDDAP's tabledap service (a flavor of OPeNDAP) for tabular (sequence) data. Add different extensions (e.g., .html, .graph, .das, .dds) to the base URL for different purposes. download [Depth profiles of quantitative PCR of ammonia-oxidizing archaea and bacteria KM1314] - Depth profiles of quantitative PCR of ammonia-oxidizing archaea and bacteria from R/V Kilo Moana KM1314 in the North Pacific Ocean (Significance of nitrification in shaping planktonic biodiversity in the ocean) 2026-07-24 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/997806 information web browser Background Information Background information from the source information originator This dataset reports depth-resolved abundances of archaeal and β-proteobacterial ammonia monooxygenase (amoA) genes collected at Stations 5, 8, and 13 in the northeastern Pacific Ocean during the R/V Kilo Moana cruise KM1314 in August 2013. One liter of seawater from each depth was filtered onto 0.22 μm Sterivex filters and stored at −80 °C prior to DNA extraction using a modified manual extraction protocol. Archaeal and bacterial amoA gene abundances were quantified by quantitative PCR using primer sets CrenAmoAQ-F/CrenAmoAModR and AmoA1-F/AmoA2-R on a LightCycler platform. Standard curves, amplification procedures, melting curve analyses, and gel electrophoresis verification followed established protocols. These measurements characterize vertical distributions of ammonia-oxidizing microorganisms in the upper 300 m of the water column and support analyses of nitrification activity and microbial community structure in the oligotrophic North Pacific. OPeNDAP 1 -150.0 -134.6803 30.0 50.0 seconds 2013-08-14T13:05:19Z 2013-08-29T14:50:20Z -300.0 -0.0 tight OPeNDAPDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_997806_v1 OPeNDAP:OPeNDAP OPeNDAP An OPeNDAP service for tabular (sequence) data. Add different extensions (e.g., .html, .das, .dds) to the base URL for different purposes. download physicalMeasurement time double Datetime_of_sampling Station_ID int Station_id AOA_amoA_Calculated_Abundance float Aoa_amoa_calculated_abundance stdev_AOA float Stdev_aoa AOB_amoA_Calculated_Abundance float Aob_amoa_calculated_abundance stdev_AOB float Stdev_aob Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org distributor OPeNDAP DAP/2.0 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_997806_v1.html order Data Subset Form ERDDAP's version of the OPeNDAP .html web page for this dataset. Specify a subset of the dataset and download the data via OPeNDAP or in many different file types. download https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_997806_v1.graph order Make-A-Graph Form ERDDAP's Make-A-Graph .html web page for this dataset. Create an image with a map or graph of a subset of the data. mapDigital This record was created from dataset metadata by ERDDAP Version 2.22