bcodmo_dataset_998771_v1 eng UTF8 dataset service Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org pointOfContact 2026-07-04 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) 3 column row vertical [Metagenome from the Pacific Ocean OMZ] - Sample information and accession numbers for metagenome samples collected in the Eastern Tropical Pacific Oxygen Minimum Zone on R/V Sally Ride cruise SR2114 during December 2021 to January 2022 (Collaborative Research: Microdiversity drives ecosystem function: SAR11 bacteria as models for oceanic nitrogen loss) 2026-07-04 creation erddap.bco-dmo.org bcodmo_dataset_998771_v1 BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/998771 information web browser Background Information Background information from the source information originator This dataset represents the shotgun metagenome samples used in used in Zhao et al., ISME J 2025. Samples for metagenomic sequencing were collected from the oxygen minimum zone in the Eastern Tropical Pacific Ocean during the R/V Sally Ride (SR2114) cruise, December 2021 – January 2022. Sea water was collected from all nine depths for all five sampling stations reported in the Zhao et al., paper (See Table for details). Collections were made using Niskin bottles on a rosette containing a conductivity–temperature–depth profiler (Sea-Bird SBE 911plus). DNA was extracted from biomass on collecting filters using the MoBio Power Soil kit (MoBio Inc. Carlsbad, CA, USA) and libraries were prepared for metagenomic sequencing using the Illumina DNA library prep kit with unique dual indexing according to manufacturer's instructions, except that the protocol was terminated after isolation of cleaned double stranded libraries. An equimolar mixture of the libraries was sequenced on an Illumina NovaSeq 6000 instrument at the Molecular Evolution Core, Georgia Institute of Technology. The shotgun metagenomes are available in the National Center for Biotechnology Information (NCBI) under BioProject number PRJNA1124864. BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/998771 information web browser Background Information Background information from the source information pointOfContact earth science atmosphere ocean biosphere biology environment theme BCO-DMO project latitude longitude depth theme CF The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO largerWorkCitation project eng geoscientificInformation 1 -107.7 -90.4497 11.8122 16.9798 -601.1 -112.0 [Metagenome from the Pacific Ocean OMZ] - Sample information and accession numbers for metagenome samples collected in the Eastern Tropical Pacific Oxygen Minimum Zone on R/V Sally Ride cruise SR2114 during December 2021 to January 2022 (Collaborative Research: Microdiversity drives ecosystem function: SAR11 bacteria as models for oceanic nitrogen loss) 2026-07-04 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/998771 information web browser Background Information Background information from the source information originator This dataset represents the shotgun metagenome samples used in used in Zhao et al., ISME J 2025. Samples for metagenomic sequencing were collected from the oxygen minimum zone in the Eastern Tropical Pacific Ocean during the R/V Sally Ride (SR2114) cruise, December 2021 – January 2022. Sea water was collected from all nine depths for all five sampling stations reported in the Zhao et al., paper (See Table for details). Collections were made using Niskin bottles on a rosette containing a conductivity–temperature–depth profiler (Sea-Bird SBE 911plus). DNA was extracted from biomass on collecting filters using the MoBio Power Soil kit (MoBio Inc. Carlsbad, CA, USA) and libraries were prepared for metagenomic sequencing using the Illumina DNA library prep kit with unique dual indexing according to manufacturer's instructions, except that the protocol was terminated after isolation of cleaned double stranded libraries. An equimolar mixture of the libraries was sequenced on an Illumina NovaSeq 6000 instrument at the Molecular Evolution Core, Georgia Institute of Technology. The shotgun metagenomes are available in the National Center for Biotechnology Information (NCBI) under BioProject number PRJNA1124864. ERDDAP tabledap 1 -107.7 -90.4497 11.8122 16.9798 -601.1 -112.0 tight ERDDAPtabledapDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_998771_v1 ERDDAP:tabledap ERDDAP-tabledap ERDDAP's tabledap service (a flavor of OPeNDAP) for tabular (sequence) data. Add different extensions (e.g., .html, .graph, .das, .dds) to the base URL for different purposes. download [Metagenome from the Pacific Ocean OMZ] - Sample information and accession numbers for metagenome samples collected in the Eastern Tropical Pacific Oxygen Minimum Zone on R/V Sally Ride cruise SR2114 during December 2021 to January 2022 (Collaborative Research: Microdiversity drives ecosystem function: SAR11 bacteria as models for oceanic nitrogen loss) 2026-07-04 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/998771 information web browser Background Information Background information from the source information originator This dataset represents the shotgun metagenome samples used in used in Zhao et al., ISME J 2025. Samples for metagenomic sequencing were collected from the oxygen minimum zone in the Eastern Tropical Pacific Ocean during the R/V Sally Ride (SR2114) cruise, December 2021 – January 2022. Sea water was collected from all nine depths for all five sampling stations reported in the Zhao et al., paper (See Table for details). Collections were made using Niskin bottles on a rosette containing a conductivity–temperature–depth profiler (Sea-Bird SBE 911plus). DNA was extracted from biomass on collecting filters using the MoBio Power Soil kit (MoBio Inc. Carlsbad, CA, USA) and libraries were prepared for metagenomic sequencing using the Illumina DNA library prep kit with unique dual indexing according to manufacturer's instructions, except that the protocol was terminated after isolation of cleaned double stranded libraries. An equimolar mixture of the libraries was sequenced on an Illumina NovaSeq 6000 instrument at the Molecular Evolution Core, Georgia Institute of Technology. The shotgun metagenomes are available in the National Center for Biotechnology Information (NCBI) under BioProject number PRJNA1124864. OPeNDAP 1 -107.7 -90.4497 11.8122 16.9798 -601.1 -112.0 tight OPeNDAPDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_998771_v1 OPeNDAP:OPeNDAP OPeNDAP An OPeNDAP service for tabular (sequence) data. Add different extensions (e.g., .html, .das, .dds) to the base URL for different purposes. download physicalMeasurement Sample_ID String Sample_id DNA_Concentration float Dna_concentration Date_Sampled String Date_sampled Date_Extracted String Date_extracted Volume_Filtered int Volume_filtered Station int Station Oxygen_Concentration float Oxygen_concentration Experiment_Accession String Experiment_accession Instrument String Instrument Study_Accession String Study_accession Sample_Accession String Sample_accession Bioproejct_number String Bioproejct_number Total_Bases int Total_bases Library_Name String Library_name Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org distributor OPeNDAP DAP/2.0 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_998771_v1.html order Data Subset Form ERDDAP's version of the OPeNDAP .html web page for this dataset. Specify a subset of the dataset and download the data via OPeNDAP or in many different file types. download https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_998771_v1.graph order Make-A-Graph Form ERDDAP's Make-A-Graph .html web page for this dataset. Create an image with a map or graph of a subset of the data. mapDigital This record was created from dataset metadata by ERDDAP Version 2.22