bcodmo_dataset_998887_v1 eng UTF8 dataset service Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org pointOfContact 2026-06-13 ISO 19115-2 Geographic Information - Metadata Part 2 Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) 3 column row vertical [SAGs from the Pacific Ocean OMZ] - Single-cell amplified genomes (SAGs) collected during CTD casts in the Eastern Tropical Pacific Ocean on R/V Atlantis cruise AT50-08 during February-March 2023 (Collaborative Research: Microdiversity drives ecosystem function: SAR11 bacteria as models for oceanic nitrogen loss) 2026-06-13 creation erddap.bco-dmo.org bcodmo_dataset_998887_v1 BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/998887 information web browser Background Information Background information from the source information originator This dataset describes assembled genomes of single-cell amplified genomes (SAGs) used in Zhao et al., ISME 2025. Water samples were collected during conductivity–temperature–depth (CTD) casts from an oxygen minimum zone in the Eastern Tropical Pacific Ocean, where the SAR11 make up ~20% of the total microbial community, during the R/V Atlantis cruise AT50-08. Sorting was performed on 4 April 2023 (within 2 months from the date the first sample was collected) and SAGs were generated with the modified genomic DNA amplification technique, WGA-Y, which enables a substantially improved average genome recovery from single cells (serviceS-202). In total, 105 SAGs with Cp values<3h were randomly selected for sequencing. Genome assembly and draft annotation were performed by Single Cell Genomics Facility (SCGC), Maine, USA as described in the center's webpage https://scgc.bigelow.org/capabilities/service-description/ and in the Zhao et al., paper. The single-cell amplified genomes are available in the National Center for Biotechnology Information (NCBI) under BioProject number PRJNA1124867. BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/998887 information web browser Background Information Background information from the source information pointOfContact earth science atmosphere ocean biosphere biology environment theme BCO-DMO project latitude longitude depth theme CF The data may be used and redistributed for free but is not intended for legal use, since it may contain inaccuracies. Neither the data Contributor, ERD, NOAA, nor the United States Government, nor any of their employees or contractors, makes any warranty, express or implied, including warranties of merchantability and fitness for a particular purpose, or assumes any legal liability for the accuracy, completeness, or usefulness, of this information. BCO-DMO largerWorkCitation project eng geoscientificInformation 1 -106.2833 -104.9 18.1667 18.8833 -1000.0 -40.0 [SAGs from the Pacific Ocean OMZ] - Single-cell amplified genomes (SAGs) collected during CTD casts in the Eastern Tropical Pacific Ocean on R/V Atlantis cruise AT50-08 during February-March 2023 (Collaborative Research: Microdiversity drives ecosystem function: SAR11 bacteria as models for oceanic nitrogen loss) 2026-06-13 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/998887 information web browser Background Information Background information from the source information originator This dataset describes assembled genomes of single-cell amplified genomes (SAGs) used in Zhao et al., ISME 2025. Water samples were collected during conductivity–temperature–depth (CTD) casts from an oxygen minimum zone in the Eastern Tropical Pacific Ocean, where the SAR11 make up ~20% of the total microbial community, during the R/V Atlantis cruise AT50-08. Sorting was performed on 4 April 2023 (within 2 months from the date the first sample was collected) and SAGs were generated with the modified genomic DNA amplification technique, WGA-Y, which enables a substantially improved average genome recovery from single cells (serviceS-202). In total, 105 SAGs with Cp values<3h were randomly selected for sequencing. Genome assembly and draft annotation were performed by Single Cell Genomics Facility (SCGC), Maine, USA as described in the center's webpage https://scgc.bigelow.org/capabilities/service-description/ and in the Zhao et al., paper. The single-cell amplified genomes are available in the National Center for Biotechnology Information (NCBI) under BioProject number PRJNA1124867. ERDDAP tabledap 1 -106.2833 -104.9 18.1667 18.8833 -1000.0 -40.0 tight ERDDAPtabledapDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_998887_v1 ERDDAP:tabledap ERDDAP-tabledap ERDDAP's tabledap service (a flavor of OPeNDAP) for tabular (sequence) data. Add different extensions (e.g., .html, .graph, .das, .dds) to the base URL for different purposes. download [SAGs from the Pacific Ocean OMZ] - Single-cell amplified genomes (SAGs) collected during CTD casts in the Eastern Tropical Pacific Ocean on R/V Atlantis cruise AT50-08 during February-March 2023 (Collaborative Research: Microdiversity drives ecosystem function: SAR11 bacteria as models for oceanic nitrogen loss) 2026-06-13 creation BCO-DMO info@bco-dmo.org https://osprey.bco-dmo.org/dataset/998887 information web browser Background Information Background information from the source information originator This dataset describes assembled genomes of single-cell amplified genomes (SAGs) used in Zhao et al., ISME 2025. Water samples were collected during conductivity–temperature–depth (CTD) casts from an oxygen minimum zone in the Eastern Tropical Pacific Ocean, where the SAR11 make up ~20% of the total microbial community, during the R/V Atlantis cruise AT50-08. Sorting was performed on 4 April 2023 (within 2 months from the date the first sample was collected) and SAGs were generated with the modified genomic DNA amplification technique, WGA-Y, which enables a substantially improved average genome recovery from single cells (serviceS-202). In total, 105 SAGs with Cp values<3h were randomly selected for sequencing. Genome assembly and draft annotation were performed by Single Cell Genomics Facility (SCGC), Maine, USA as described in the center's webpage https://scgc.bigelow.org/capabilities/service-description/ and in the Zhao et al., paper. The single-cell amplified genomes are available in the National Center for Biotechnology Information (NCBI) under BioProject number PRJNA1124867. OPeNDAP 1 -106.2833 -104.9 18.1667 18.8833 -1000.0 -40.0 tight OPeNDAPDatasetQueryAndAccess https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_998887_v1 OPeNDAP:OPeNDAP OPeNDAP An OPeNDAP service for tabular (sequence) data. Add different extensions (e.g., .html, .das, .dds) to the base URL for different purposes. download physicalMeasurement SAG_identifier String Sag_identifier Station String Station Collection_date String Collection_date Genome String Genome Clade String Clade Completeness float Completeness Contamination float Contamination Strain_heterogeneity int Strain_heterogeneity Experiment_Accession String Experiment_accession Study_Accession String Study_accession Sample_Accession String Sample_accession SAG_ID String Sag_id Adam Shepherd BCO-DMO 508-289-2772 266 Woods Hole Rd. MS#36 Woods Hole MA 02543 USA erddap@bco-dmo.org distributor OPeNDAP DAP/2.0 https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_998887_v1.html order Data Subset Form ERDDAP's version of the OPeNDAP .html web page for this dataset. Specify a subset of the dataset and download the data via OPeNDAP or in many different file types. download https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_998887_v1.graph order Make-A-Graph Form ERDDAP's Make-A-Graph .html web page for this dataset. Create an image with a map or graph of a subset of the data. mapDigital This record was created from dataset metadata by ERDDAP Version 2.22