ERDDAP > info > bcodmo_dataset_3719

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[sea_fan_parasite_growth] - Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient conducted at the Harvell lab at Cornell University (Influence of Temperature and Acidification on the Dynamics of Coral Co-Infection and Resistance)		I   M	background			BCO-DMO	bcodmo_dataset_3719

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Sampling and Analytical Methodology: In the first trial, a Labyrinthulomycota culture held at 22 degrees C was divided into 18 sub-cultures that were incubated at 15 degrees C, 20 degrees C, 25 degrees C, 30 degrees C, and 32 degrees C in triplicate for three days. Culture temperatures and incubation period were based on previous visual observations of Labyrinthulomycota growth, where over-growth of culture flasks occurs after three days at temperatures of 25 degrees C and higher. In the second trial, nine sub-cultures were incubated at 20 degrees C, 25 degrees C, and 30 degrees C in triplicate for three days. In the second trial, total protein was also assessed. After three days, the media was poured off, rinsed once with 3 mLs of 0.22 um-filtered artificial sea water, and replaced with 3 mLs of 0.22 um-filtered artificial sea water. With a sterile wooden dowel, the bottom of each culture was scraped until no Labyrinthulomycota growth was visible on the bottom of the culture. 700 uL of each culture was placed in a bead beater and mixed at 300 rpm for 30 sec; 400 uL was set aside for protein assays and 300 uL for cell counts and held on ice until use. Prior to counting using a hemocytometer, cells were vortexed for about 20 sec. In each culture, cells were counted in triplicate. Total protein was extracted from each sample by adding 400 uL of extraction buffer (0.15 ug mL-1 DTT in Tris-HCl) to each tube. The contents of the tube were mixed and lysed for 2 minutes with the Fisherbrand disposable pestle grinder system, and incubated for 45 minutes on ice for extractions. Protein was measured using the DC protein kit (Bio-Rad), and read in triplicate using the Synergy HT multi-Detection microplate reader with KC4 software (Biotek Instruments, Vermont) at 750 nm.
attribute	NC_GLOBAL	awards_0_award_nid	String	55012
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-0849776
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0849776
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Growth of Aplanochytrium cells over a temperature gradient PI: Drew Harvell (Cornell University) Co-PI: Laura Mydlarz (University of Texas Arlington) Version: 12 Sept 2012
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2012-09-12T18:15:39Z
attribute	NC_GLOBAL	date_modified	String	2019-03-01T21:25:08Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.3719.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/3719
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	keywords	String	average, bco, bco-dmo, biological, chemical, count, count_avg, count_avg_se, count_log10, count_log10_avg, count_log10_avg_se, data, dataset, dmo, erddap, log10, management, oceanography, office, preliminary, protein, protein_avg, protein_avg_se, protein_log10, protein_log10_avg, protein_log10_avg_se, rep, temperature, trial
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/3719/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/3719
attribute	NC_GLOBAL	param_mapping	String	{'3719': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/3719/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Cornell University
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	Cornell
attribute	NC_GLOBAL	people_0_person_name	String	Drew Harvell
attribute	NC_GLOBAL	people_0_person_nid	String	51556
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of Texas at Arlington
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	UT Arlington
attribute	NC_GLOBAL	people_1_person_name	String	Dr Laura Mydlarz
attribute	NC_GLOBAL	people_1_person_nid	String	51558
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	Cornell University
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	Cornell
attribute	NC_GLOBAL	people_2_person_name	String	Dr Colleen Burge
attribute	NC_GLOBAL	people_2_person_nid	String	51557
attribute	NC_GLOBAL	people_2_role	String	Contact
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_3_person_nid	String	51498
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	Climate_CoralDisease
attribute	NC_GLOBAL	projects_0_acronym	String	Climate_CoralDisease
attribute	NC_GLOBAL	projects_0_description	String	This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5). Coral reef ecosystems are highly endangered by recent increases in temperature and by projected increases in ocean acidification. Although temperature has been identified as a driver of some coral disease outbreaks, nothing is known about direct effects of acidification on host immunity and pathogen virulence, or the potential for synergism with temperature. Natural coral populations often suffer from simultaneous infection by multiple pathogens that can also influence host immune responses, but co-infection dynamics have not been investigated in invertebrate systems lacking classical adaptive immunity. Changing climate will very likely influence the outcome of single and co-infection. This project will investigate the influence of environmental stress on co-infection dynamics of the sea fan coral, Gorgonia ventalina, with a fungal pathogen, Aspergillus sydowii and a protist parasite, SPX. The goal is to identify the mechanisms through which multiple infections, temperature and acidification modify host resistance, leading to changes in within- and among-colony rates of disease spread. The objectives of this project are to: (1) Identify incidence and co-infection frequency of Aspergillus sydowii and SPX. Detailed field surveys of the two diseases will test the hypothesis that co-infection is significant, provide valuable information about drivers of aspergillosis, and will help to characterize an emerging new sea fan disease. (2) Investigate how co-infection influences sea fan susceptibility, resistance, and within host disease dynamics. Through manipulative lab inoculation experiments we will test the hypothesis that single infections increase susceptibility to a second pathogen. (3) Examine the effects of temperature increase and ocean acidification on pathogen virulence, on underlying host resistance, and on the dynamics of single and co-infections. The hypotheses that acidification will increase pathogen virulence and host susceptibility will be tested in a temperature and pH controlled experimental system. This system will also allow the potential synergistic effects of temperature and acidification on host immunity and co-infection dynamics to be explored. The primary intellectual merit of the proposed work will be a greater understanding of how changing climate mediates co-infection and immunity in a non-model invertebrate. While fungal pathogens are primarily opportunistic, labyrinthulid protozoans are recognized as primary pathogens in shellfish. Even in shellfish, little is known about co-infections involving labyrinthulids, and these protists are entirely unstudied in corals. Publications associated with this project: Burge CA, Douglas N, Conti-Jerpe I, Weil E, Roberts S, Friedman CS & CD Harvell. (May 2012) Friend or foe: the association of Labyrinthulomycetes with the Caribbean sea fan, Gorgonia ventalina. Dis Aquat Org. 101:1-12. doi: 10.3354/dao02487 Burge CA, Mouchka, ME, Harvell, CD & S Roberts. (In review) Immune response of the Caribbean sea fan, Gorgonia ventalina exposed to an Aplanochytrium parasite as revealed by transcriptome sequencing.
attribute	NC_GLOBAL	projects_0_end_date	String	2012-07
attribute	NC_GLOBAL	projects_0_geolocation	String	Florida Keys & Puerto Rico
attribute	NC_GLOBAL	projects_0_name	String	Influence of Temperature and Acidification on the Dynamics of Coral Co-Infection and Resistance
attribute	NC_GLOBAL	projects_0_project_nid	String	2232
attribute	NC_GLOBAL	projects_0_start_date	String	2009-08
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient. Two types of assays were used in two trials to quantify Labyrinthulomycota cultures: cell counts using a hemocytometer and total protein concentration.
attribute	NC_GLOBAL	title	String	[sea_fan_parasite_growth] - Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient conducted at the Harvell lab at Cornell University (Influence of Temperature and Acidification on the Dynamics of Coral Co-Infection and Resistance)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	trial		byte
attribute	trial	_FillValue	byte	127
attribute	trial	actual_range	byte	1, 2
attribute	trial	bcodmo_name	String	exp_id
attribute	trial	description	String	Experimental trial number (1 or 2; see Acquisition Description).
attribute	trial	long_name	String	Trial
attribute	trial	units	String	unitless
variable	temp		byte
attribute	temp	_FillValue	byte	127
attribute	temp	actual_range	byte	15, 32
attribute	temp	bcodmo_name	String	unknown
attribute	temp	description	String	Incubation temperature.
attribute	temp	long_name	String	Temperature
attribute	temp	units	String	degrees C
variable	count_avg		double
attribute	count_avg	_FillValue	double	NaN
attribute	count_avg	actual_range	double	0.0, 5263333.33
attribute	count_avg	bcodmo_name	String	count
attribute	count_avg	colorBarMaximum	double	100.0
attribute	count_avg	colorBarMinimum	double	0.0
attribute	count_avg	description	String	Average of total cells per temperature.
attribute	count_avg	long_name	String	Count Avg
attribute	count_avg	units	String	# cells (unitless)
variable	count_avg_se		double
attribute	count_avg_se	_FillValue	double	NaN
attribute	count_avg_se	actual_range	double	0.0, 663498.42
attribute	count_avg_se	bcodmo_name	String	unknown
attribute	count_avg_se	colorBarMaximum	double	100.0
attribute	count_avg_se	colorBarMinimum	double	0.0
attribute	count_avg_se	description	String	Standard error of count_avg.
attribute	count_avg_se	long_name	String	Count Avg Se
attribute	count_avg_se	units	String	# cells (unitless)
variable	count_log10_avg		double
attribute	count_log10_avg	_FillValue	double	NaN
attribute	count_log10_avg	actual_range	double	0.0, 6.707714392
attribute	count_log10_avg	bcodmo_name	String	count
attribute	count_log10_avg	colorBarMaximum	double	100.0
attribute	count_log10_avg	colorBarMinimum	double	0.0
attribute	count_log10_avg	description	String	Average of log 10 transformed cell counts per temperature.
attribute	count_log10_avg	long_name	String	Count Log10 Avg
attribute	count_log10_avg	units	String	# cells (unitless)
variable	count_log10_avg_se		double
attribute	count_log10_avg_se	_FillValue	double	NaN
attribute	count_log10_avg_se	actual_range	double	0.0, 0.14726115
attribute	count_log10_avg_se	bcodmo_name	String	unknown
attribute	count_log10_avg_se	colorBarMaximum	double	100.0
attribute	count_log10_avg_se	colorBarMinimum	double	0.0
attribute	count_log10_avg_se	description	String	Standard error of count_log10_avg.
attribute	count_log10_avg_se	long_name	String	Count Log10 Avg Se
attribute	count_log10_avg_se	units	String	# cells (unitless)
variable	protein_avg		double
attribute	protein_avg	_FillValue	double	NaN
attribute	protein_avg	actual_range	double	8.56, 11.09937689
attribute	protein_avg	bcodmo_name	String	unknown
attribute	protein_avg	description	String	Average protein concentration per temperature.
attribute	protein_avg	long_name	String	Protein Avg
attribute	protein_avg	units	String	mg protein per culture
variable	protein_avg_se		double
attribute	protein_avg_se	_FillValue	double	NaN
attribute	protein_avg_se	actual_range	double	0.50994737, 0.89
attribute	protein_avg_se	bcodmo_name	String	unknown
attribute	protein_avg_se	description	String	Standard error of protein_avg.
attribute	protein_avg_se	long_name	String	Protein Avg Se
attribute	protein_avg_se	units	String	mg protein per culture
variable	protein_log10_avg		double
attribute	protein_log10_avg	_FillValue	double	NaN
attribute	protein_log10_avg	actual_range	double	0.93, 1.040367223
attribute	protein_log10_avg	bcodmo_name	String	unknown
attribute	protein_log10_avg	description	String	Average of log 10 transformed protein concentration per temperature.
attribute	protein_log10_avg	long_name	String	Protein Log10 Avg
attribute	protein_log10_avg	units	String	mg protein per culture
variable	protein_log10_avg_se		double
attribute	protein_log10_avg_se	_FillValue	double	NaN
attribute	protein_log10_avg_se	actual_range	double	0.025478283, 0.05
attribute	protein_log10_avg_se	bcodmo_name	String	unknown
attribute	protein_log10_avg_se	description	String	Standard error of protein_log10_avg.
attribute	protein_log10_avg_se	long_name	String	Protein Log10 Avg Se
attribute	protein_log10_avg_se	units	String	mg protein per culture
variable	rep		byte
attribute	rep	_FillValue	byte	127
attribute	rep	actual_range	byte	1, 3
attribute	rep	bcodmo_name	String	unknown
attribute	rep	description	String	Replicate.
attribute	rep	long_name	String	Rep
attribute	rep	units	String	unitless
variable	count		int
attribute	count	_FillValue	int	2147483647
attribute	count	actual_range	int	0, 8670000
attribute	count	bcodmo_name	String	count
attribute	count	colorBarMaximum	double	100.0
attribute	count	colorBarMinimum	double	0.0
attribute	count	description	String	Total number of cells per culture.
attribute	count	long_name	String	Count
attribute	count	units	String	# cells (unitless)
variable	protein		double
attribute	protein	_FillValue	double	NaN
attribute	protein	actual_range	double	5.839496, 13.382512
attribute	protein	bcodmo_name	String	unknown
attribute	protein	description	String	Protein concentration measured in milligrams of protein per culture.
attribute	protein	long_name	String	Protein
attribute	protein	units	String	mg protein per culture
variable	count_log10		double
attribute	count_log10	_FillValue	double	NaN
attribute	count_log10	actual_range	double	0.0, 6.938019097
attribute	count_log10	bcodmo_name	String	count
attribute	count_log10	colorBarMaximum	double	100.0
attribute	count_log10	colorBarMinimum	double	0.0
attribute	count_log10	description	String	Total cells per culture, log 10 transformed.
attribute	count_log10	long_name	String	Count Log10
attribute	count_log10	units	String	# cells (unitless)
variable	protein_log10		double
attribute	protein_log10	_FillValue	double	NaN
attribute	protein_log10	actual_range	double	0.766375365, 1.126537641
attribute	protein_log10	bcodmo_name	String	unknown
attribute	protein_log10	description	String	Protein concentration, log 10 transformed.
attribute	protein_log10	long_name	String	Protein Log10
attribute	protein_log10	units	String	mg protein per culture

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.