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Title Sum-
Institution Dataset ID
     data   graph     files  public Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a
temperature gradient conducted at the Harvell lab at Cornell University
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv
attribute NC_GLOBAL acquisition_description String Sampling and Analytical Methodology:
In the first trial, a Labyrinthulomycota culture held at 22 degrees C was
divided into 18 sub-cultures that were incubated at 15 degrees C, 20 degrees
C, 25 degrees C, 30 degrees C, and 32 degrees C in triplicate for three days.
Culture temperatures and incubation period were based on previous visual
observations of Labyrinthulomycota growth, where over-growth of culture flasks
occurs after three days at temperatures of 25 degrees C and higher. In the
second trial, nine sub-cultures were incubated at 20 degrees C, 25 degrees C,
and 30 degrees C in triplicate for three days.

In the second trial, total protein was also assessed. After three days, the
media was poured off, rinsed once with 3 mLs of 0.22 um-filtered artificial
sea water, and replaced with 3 mLs of 0.22 um-filtered artificial sea water.
With a sterile wooden dowel, the bottom of each culture was scraped until no
Labyrinthulomycota growth was visible on the bottom of the culture. 700 uL of
each culture was placed in a bead beater and mixed at 300 rpm for 30 sec; 400
uL was set aside for protein assays and 300 uL for cell counts and held on ice
until use.

Prior to counting using a hemocytometer, cells were vortexed for about 20 sec.
In each culture, cells were counted in triplicate.

Total protein was extracted from each sample by adding 400 uL of extraction
buffer (0.15 ug mL-1 DTT in Tris-HCl) to each tube. The contents of the tube
were mixed and lysed for 2 minutes with the Fisherbrand disposable pestle
grinder system, and incubated for 45 minutes on ice for extractions. Protein
was measured using the DC protein kit (Bio-Rad), and read in triplicate using
the Synergy HT multi-Detection microplate reader with KC4 software (Biotek
Instruments, Vermont) at 750 nm.
attribute NC_GLOBAL awards_0_award_nid String 55012
attribute NC_GLOBAL awards_0_award_number String OCE-0849776
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0849776 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Growth of Aplanochytrium cells over a temperature gradient
PI: Drew Harvell (Cornell University)
Co-PI: Laura Mydlarz (University of Texas Arlington)
Version: 12 Sept 2012
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2012-09-12T18:15:39Z
attribute NC_GLOBAL date_modified String 2019-03-01T21:25:08Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.3719.1
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/3719 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL keywords String average, bco, bco-dmo, biological, chemical, count, count_avg, count_avg_se, count_log10, count_log10_avg, count_log10_avg_se, data, dataset, dmo, erddap, log10, management, oceanography, office, preliminary, protein, protein_avg, protein_avg_se, protein_log10, protein_log10_avg, protein_log10_avg_se, rep, temperature, trial
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/3719/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/3719 (external link)
attribute NC_GLOBAL param_mapping String {'3719': {}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/3719/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Cornell University
attribute NC_GLOBAL people_0_affiliation_acronym String Cornell
attribute NC_GLOBAL people_0_person_name String Drew Harvell
attribute NC_GLOBAL people_0_person_nid String 51556
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Texas at Arlington
attribute NC_GLOBAL people_1_affiliation_acronym String UT Arlington
attribute NC_GLOBAL people_1_person_name String Dr Laura Mydlarz
attribute NC_GLOBAL people_1_person_nid String 51558
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String Cornell University
attribute NC_GLOBAL people_2_affiliation_acronym String Cornell
attribute NC_GLOBAL people_2_person_name String Dr Colleen Burge
attribute NC_GLOBAL people_2_person_nid String 51557
attribute NC_GLOBAL people_2_role String Contact
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Shannon Rauch
attribute NC_GLOBAL people_3_person_nid String 51498
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String Climate_CoralDisease
attribute NC_GLOBAL projects_0_acronym String Climate_CoralDisease
attribute NC_GLOBAL projects_0_description String This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).
Coral reef ecosystems are highly endangered by recent increases in temperature and by projected increases in ocean acidification. Although temperature has been identified as a driver of some coral disease outbreaks, nothing is known about direct effects of acidification on host immunity and pathogen virulence, or the potential for synergism with temperature. Natural coral populations often suffer from simultaneous infection by multiple pathogens that can also influence host immune responses, but co-infection dynamics have not been investigated in invertebrate systems lacking classical adaptive immunity. Changing climate will very likely influence the outcome of single and co-infection.
This project will investigate the influence of environmental stress on co-infection dynamics of the sea fan coral, Gorgonia ventalina, with a fungal pathogen, Aspergillus sydowii and a protist parasite, SPX. The goal is to identify the mechanisms through which multiple infections, temperature and acidification modify host resistance, leading to changes in within- and among-colony rates of disease spread.
The objectives of this project are to:
(1) Identify incidence and co-infection frequency of Aspergillus sydowii and SPX. Detailed field surveys of the two diseases will test the hypothesis that co-infection is significant, provide valuable information about drivers of aspergillosis, and will help to characterize an emerging new sea fan disease.
(2) Investigate how co-infection influences sea fan susceptibility, resistance, and within host disease dynamics. Through manipulative lab inoculation experiments we will test the hypothesis that single infections increase susceptibility to a second pathogen.
(3) Examine the effects of temperature increase and ocean acidification on pathogen virulence, on underlying host resistance, and on the dynamics of single and co-infections.
The hypotheses that acidification will increase pathogen virulence and host susceptibility will be tested in a temperature and pH controlled experimental system. This system will also allow the potential synergistic effects of temperature and acidification on host immunity and co-infection dynamics to be explored. The primary intellectual merit of the proposed work will be a greater understanding of how changing climate mediates co-infection and immunity in a non-model invertebrate. While fungal pathogens are primarily opportunistic, labyrinthulid protozoans are recognized as primary pathogens in shellfish. Even in shellfish, little is known about co-infections involving labyrinthulids, and these protists are entirely unstudied in corals.
Publications associated with this project:
Burge CA, Douglas N, Conti-Jerpe I, Weil E, Roberts S, Friedman CS & CD Harvell. (May 2012) Friend or foe: the association of Labyrinthulomycetes with the Caribbean sea fan, Gorgonia ventalina. Dis Aquat Org. 101:1-12. doi: 10.3354/dao02487
Burge CA, Mouchka, ME, Harvell, CD & S Roberts. (In review) Immune response of the Caribbean sea fan, Gorgonia ventalina exposed to an Aplanochytrium parasite as revealed by transcriptome sequencing.
attribute NC_GLOBAL projects_0_end_date String 2012-07
attribute NC_GLOBAL projects_0_geolocation String Florida Keys & Puerto Rico
attribute NC_GLOBAL projects_0_name String Influence of Temperature and Acidification on the Dynamics of Coral Co-Infection and Resistance
attribute NC_GLOBAL projects_0_project_nid String 2232
attribute NC_GLOBAL projects_0_start_date String 2009-08
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient. Two types of assays were used in two trials to quantify Labyrinthulomycota cultures: cell counts using a hemocytometer and total protein concentration.
attribute NC_GLOBAL title String Experimental results on the growth of Aplanochytrium (a sea fan parasite) cells over a temperature gradient conducted at the Harvell lab at Cornell University
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable trial   byte  
attribute trial _FillValue byte 127
attribute trial actual_range byte 1, 2
attribute trial bcodmo_name String exp_id
attribute trial description String Experimental trial number (1 or 2; see Acquisition Description).
attribute trial long_name String Trial
attribute trial units String unitless
variable temp   byte  
attribute temp _FillValue byte 127
attribute temp actual_range byte 15, 32
attribute temp bcodmo_name String unknown
attribute temp description String Incubation temperature.
attribute temp long_name String Temperature
attribute temp units String degrees C
variable count_avg   double  
attribute count_avg _FillValue double NaN
attribute count_avg actual_range double 0.0, 5263333.33
attribute count_avg bcodmo_name String count
attribute count_avg colorBarMaximum double 100.0
attribute count_avg colorBarMinimum double 0.0
attribute count_avg description String Average of total cells per temperature.
attribute count_avg long_name String Count Avg
attribute count_avg units String # cells (unitless)
variable count_avg_se   double  
attribute count_avg_se _FillValue double NaN
attribute count_avg_se actual_range double 0.0, 663498.42
attribute count_avg_se bcodmo_name String unknown
attribute count_avg_se colorBarMaximum double 100.0
attribute count_avg_se colorBarMinimum double 0.0
attribute count_avg_se description String Standard error of count_avg.
attribute count_avg_se long_name String Count Avg Se
attribute count_avg_se units String # cells (unitless)
variable count_log10_avg   double  
attribute count_log10_avg _FillValue double NaN
attribute count_log10_avg actual_range double 0.0, 6.707714392
attribute count_log10_avg bcodmo_name String count
attribute count_log10_avg colorBarMaximum double 100.0
attribute count_log10_avg colorBarMinimum double 0.0
attribute count_log10_avg description String Average of log 10 transformed cell counts per temperature.
attribute count_log10_avg long_name String Count Log10 Avg
attribute count_log10_avg units String # cells (unitless)
variable count_log10_avg_se   double  
attribute count_log10_avg_se _FillValue double NaN
attribute count_log10_avg_se actual_range double 0.0, 0.14726115
attribute count_log10_avg_se bcodmo_name String unknown
attribute count_log10_avg_se colorBarMaximum double 100.0
attribute count_log10_avg_se colorBarMinimum double 0.0
attribute count_log10_avg_se description String Standard error of count_log10_avg.
attribute count_log10_avg_se long_name String Count Log10 Avg Se
attribute count_log10_avg_se units String # cells (unitless)
variable protein_avg   double  
attribute protein_avg _FillValue double NaN
attribute protein_avg actual_range double 8.56, 11.09937689
attribute protein_avg bcodmo_name String unknown
attribute protein_avg description String Average protein concentration per temperature.
attribute protein_avg long_name String Protein Avg
attribute protein_avg units String mg protein per culture
variable protein_avg_se   double  
attribute protein_avg_se _FillValue double NaN
attribute protein_avg_se actual_range double 0.50994737, 0.89
attribute protein_avg_se bcodmo_name String unknown
attribute protein_avg_se description String Standard error of protein_avg.
attribute protein_avg_se long_name String Protein Avg Se
attribute protein_avg_se units String mg protein per culture
variable protein_log10_avg   double  
attribute protein_log10_avg _FillValue double NaN
attribute protein_log10_avg actual_range double 0.93, 1.040367223
attribute protein_log10_avg bcodmo_name String unknown
attribute protein_log10_avg description String Average of log 10 transformed protein concentration per temperature.
attribute protein_log10_avg long_name String Protein Log10 Avg
attribute protein_log10_avg units String mg protein per culture
variable protein_log10_avg_se   double  
attribute protein_log10_avg_se _FillValue double NaN
attribute protein_log10_avg_se actual_range double 0.025478283, 0.05
attribute protein_log10_avg_se bcodmo_name String unknown
attribute protein_log10_avg_se description String Standard error of protein_log10_avg.
attribute protein_log10_avg_se long_name String Protein Log10 Avg Se
attribute protein_log10_avg_se units String mg protein per culture
variable rep   byte  
attribute rep _FillValue byte 127
attribute rep actual_range byte 1, 3
attribute rep bcodmo_name String unknown
attribute rep description String Replicate.
attribute rep long_name String Rep
attribute rep units String unitless
variable count   int  
attribute count _FillValue int 2147483647
attribute count actual_range int 0, 8670000
attribute count bcodmo_name String count
attribute count colorBarMaximum double 100.0
attribute count colorBarMinimum double 0.0
attribute count description String Total number of cells per culture.
attribute count long_name String Count
attribute count units String # cells (unitless)
variable protein   double  
attribute protein _FillValue double NaN
attribute protein actual_range double 5.839496, 13.382512
attribute protein bcodmo_name String unknown
attribute protein description String Protein concentration measured in milligrams of protein per culture.
attribute protein long_name String Protein
attribute protein units String mg protein per culture
variable count_log10   double  
attribute count_log10 _FillValue double NaN
attribute count_log10 actual_range double 0.0, 6.938019097
attribute count_log10 bcodmo_name String count
attribute count_log10 colorBarMaximum double 100.0
attribute count_log10 colorBarMinimum double 0.0
attribute count_log10 description String Total cells per culture, log 10 transformed.
attribute count_log10 long_name String Count Log10
attribute count_log10 units String # cells (unitless)
variable protein_log10   double  
attribute protein_log10 _FillValue double NaN
attribute protein_log10 actual_range double 0.766375365, 1.126537641
attribute protein_log10 bcodmo_name String unknown
attribute protein_log10 description String Protein concentration, log 10 transformed.
attribute protein_log10 long_name String Protein Log10
attribute protein_log10 units String mg protein per culture

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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