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   set  data   graph     files  public [fluor_PAM] - Pulse Amplitude Modulation (PAM) fluorometry readings from microcosm
experiments from samples collected by R/V E.O. Wilson in the Gulf of Mexico, Alabama (En-Gen
DMSP Cycling project) (En-Gen: A Functional Genomics Approach to Organic Sulfur Cycling in the
Ocean)
   ?   F   I   M   background (external link) RSS Subscribe BCO-DMO bcodmo_dataset_3873

The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String See Howard et al. 2010 and Rinta-Kanto et al. 2011 for detailed methods,
summarized below:

\"In October 2006, seawater was collected from surface waters (<1 m deep) in
the Gulf of Mexico off the coast of Dauphin Island, AL (lat: 30 03.041N; lon:
87 59.708W). Water was filtered through a 200-um mesh into six 20-liter
polyethylene Cubitainers with minimal headspace.

Three microcosms were amended with 10 um sodium nitrate (NaNO3) and 0.6 um
potassium phosphate (K2HPO4) to serve as the experimental microcosms. Three
microcosms were left untreated to serve as the control. The Cubitainers were
maintained at 27 degrees C on a 12-hour light/dark cycle for the duration of
the experiment.

Fv:Fm ratios were measured using a Walz Water PAM (pulse amplitude modulation)
fluorometer.\"

Experimental design is further described in:
E. C. Howard, S. Sun, C. R. Reisch, D. A. del Valle, R. P. Kiene, and M. A.
Moran (2010). Changes in DMSP Demethylase Gene Assemblages in Response to an
Induced Phytoplankton Bloom. Applied and Environmental Microbiology, vol. 77,
p. 524. DOI:
[10.1128/AEM.01457-10](\\"https://dx.doi.org/10.1128/AEM.01457-10\\")
Rinta-Kanto, H. Burgmann, S. M. Gifford, S. Sun, S. Sharma, R. P. Kiene, and
M. A. Moran (2011). Analysis of Sulfur-Related Gene Expression by Roseobacter
Communities Using a Taxon-Specific Functional Gene Microarray. Environmental
Microbiology, vol. 13, p. 453. DOI:
[10.1111/j.1462-2920.2010.02350.x](\\"https://dx.doi.org/10.1111/j.1462-2920.2010.02350.x\\")
attribute NC_GLOBAL awards_0_award_nid String 55114
attribute NC_GLOBAL awards_0_award_number String OCE-0724017
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0724017 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Pulse Amplitude Modulation (PAM) fluorometer readings from microcosm experiments
PI: Mary Ann Moran (UGA); Co-PIs: Ronald Kiene (DISL) and William Whitman (UGA)
Version: 19 Nov 2012
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2013-02-05T15:12:29Z
attribute NC_GLOBAL date_modified String 2019-11-01T15:25:52Z
attribute NC_GLOBAL defaultDataQuery String &amp;time&lt;now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.3873.1
attribute NC_GLOBAL Easternmost_Easting double -87.99513
attribute NC_GLOBAL geospatial_lat_max double 30.05068
attribute NC_GLOBAL geospatial_lat_min double 30.05068
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -87.99513
attribute NC_GLOBAL geospatial_lon_min double -87.99513
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/3873 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Fluorometer
attribute NC_GLOBAL instruments_0_dataset_instrument_description String A Walz Water PAM (pulse amplitude modulation) fluorometer was used to determine Fv:Fm. PAM fluorometers emit a range of pulsed lights to measure photosynthesis. The pulses of light are modulated, and the amplitude of the pulse of light governs its intensity. See more information from the manufacturer.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 6091
attribute NC_GLOBAL instruments_0_description String A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/113/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Fluorometer
attribute NC_GLOBAL instruments_0_instrument_nid String 484
attribute NC_GLOBAL instruments_0_supplied_name String Fluorometer
attribute NC_GLOBAL instruments_1_acronym String bucket
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Water was collected in the field using a clean bucket.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 6092
attribute NC_GLOBAL instruments_1_description String A bucket used to collect surface sea water samples.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0536/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String bucket
attribute NC_GLOBAL instruments_1_instrument_nid String 569
attribute NC_GLOBAL instruments_1_supplied_name String bucket
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, chemical, comments, data, dataset, day, desc, dmo, erddap, exp, exp_day, exp_id, Fv_to_Fm, Fv_to_Fm_mean, Fv_to_Fm_sd, latitude, longitude, management, mean, microcosm, microcosm_type, month, oceanography, office, preliminary, site, site_desc, type, year
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/3873/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/3873 (external link)
attribute NC_GLOBAL Northernmost_Northing double 30.05068
attribute NC_GLOBAL param_mapping String {'3873': {'lat': 'master - latitude', 'lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/3873/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of Georgia
attribute NC_GLOBAL people_0_affiliation_acronym String UGA
attribute NC_GLOBAL people_0_person_name String Mary Ann Moran
attribute NC_GLOBAL people_0_person_nid String 51592
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Dauphin Island Sea Lab
attribute NC_GLOBAL people_1_affiliation_acronym String DISL
attribute NC_GLOBAL people_1_person_name String Ronald Kiene
attribute NC_GLOBAL people_1_person_nid String 51594
attribute NC_GLOBAL people_1_role String Co-Principal Investigator
attribute NC_GLOBAL people_1_role_type String originator
attribute NC_GLOBAL people_2_affiliation String University of Georgia
attribute NC_GLOBAL people_2_affiliation_acronym String UGA
attribute NC_GLOBAL people_2_person_name String William Whitman
attribute NC_GLOBAL people_2_person_nid String 51593
attribute NC_GLOBAL people_2_role String Co-Principal Investigator
attribute NC_GLOBAL people_2_role_type String originator
attribute NC_GLOBAL people_3_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_3_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_3_person_name String Shannon Rauch
attribute NC_GLOBAL people_3_person_nid String 51498
attribute NC_GLOBAL people_3_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_3_role_type String related
attribute NC_GLOBAL project String En-Gen DMSP Cycling
attribute NC_GLOBAL projects_0_acronym String En-Gen DMSP Cycling
attribute NC_GLOBAL projects_0_description String The recent discovery of key genes that mediate competing pathways at a critical juncture in the marine sulfur cycle has allowed biogeochemists to make rapid advances in understanding where and when sulfur transformations occur in the ocean, and most importantly, what factors regulate them. This project describes an environmental functional genomics project that will rapidly increase our knowledge of the role that bacterioplankton play in dimethylsulfoniopropionate (DMSP) cycling in ocean surface waters, focusing particularly on biological controls of volatile sulfur exchange across the ocean/atmosphere boundary.
The investigators have asked three critical hypotheses to explain the regulation of bacterial DMSP degradation: that involve investigations on the energy constraints of DMSP cycling, the role that DMSP concentration in the oceans plays, and the sulfur requirements for bacterial growth. These research areas serve as the focus for hypothesis-driven laboratory and field studies using functional genomics approaches that will track patterns in gene expression in relation to sulfur metabolism. The hypotheses will be tested with:
1) chemostat systems with a model marine bacterium Silicibacter pomeroyi;
2) microcosm experiments with Gulf of Mexico seawater; and
3) field studies at various sites in the Gulf of Mexico. Marine bacterioplankton play a key role in regulating the flux of DMSP-derived sulfur to the atmosphere, a process of great importance for global climate regulation and marine productivity.
The investigators will also be involved in graduate and undergraduate student education, and two post-doctoral associates will be trained to address multidisciplinary challenges in environmental microbiology. High school biology students in Athens, GA will participate in marine microbial biology research that includes bacterial diversity and discovery studies in coastal Georgia, follow-up training in molecular tools and bioinformatics in their own classroom, and summer internships at the University of Georgia and Dauphin Island Sea Laboratory.
(The description above is from the NSF Award Abstract).
attribute NC_GLOBAL projects_0_end_date String 2012-09
attribute NC_GLOBAL projects_0_geolocation String Sapelo Island, GA, USA, 31.4° N Lat, 81.3° W Lon / Dauphin Island, AL, USA, 30.3 ° N Lat, 88.1° W Lon
attribute NC_GLOBAL projects_0_name String En-Gen: A Functional Genomics Approach to Organic Sulfur Cycling in the Ocean
attribute NC_GLOBAL projects_0_project_nid String 2244
attribute NC_GLOBAL projects_0_start_date String 2007-10
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 30.05068
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL subsetVariables String exp_id,latitude,longitude,site_desc,month,year
attribute NC_GLOBAL summary String Fv:Fm ratios from control and experimental microcosms from the Dauphin Island Cubitainer Experiment (DICE) measured using a Pulse Amplitude Modulation (PAM) fluorometer.
attribute NC_GLOBAL title String [fluor_PAM] - Pulse Amplitude Modulation (PAM) fluorometry readings from microcosm experiments from samples collected by R/V E.O. Wilson in the Gulf of Mexico, Alabama (En-Gen DMSP Cycling project) (En-Gen: A Functional Genomics Approach to Organic Sulfur Cycling in the Ocean)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -87.99513
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable exp_id   String  
attribute exp_id bcodmo_name String exp_id
attribute exp_id description String Name of the experiment. DICE = Dauphin Island Cubitainer Experiment.
attribute exp_id long_name String Exp Id
attribute exp_id units String text
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 30.05068, 30.05068
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude of the collection site. North = Positive.
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -87.99513, -87.99513
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude of the collection site. West = Negative.
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable site_desc   String  
attribute site_desc bcodmo_name String site_descrip
attribute site_desc description String Description of the sample collection site.
attribute site_desc long_name String Site Desc
attribute site_desc units String text
variable exp_day   String  
attribute exp_day bcodmo_name String unknown
attribute exp_day description String Day of the experiment. First experimental day = T0.
attribute exp_day long_name String Exp Day
attribute exp_day units String unitless
variable month   byte  
attribute month _FillValue byte 127
attribute month actual_range byte 10, 10
attribute month bcodmo_name String month
attribute month description String 2-digit month of year.
attribute month long_name String Month
attribute month nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/MNTHXXXX/ (external link)
attribute month units String mm (01 to 12)
variable day   byte  
attribute day _FillValue byte 127
attribute day actual_range byte 10, 16
attribute day bcodmo_name String day
attribute day description String 2-digit day of month.
attribute day long_name String Day
attribute day nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/DAYXXXXX/ (external link)
attribute day units String dd (01 to 31)
variable year   short  
attribute year _FillValue short 32767
attribute year actual_range short 2006, 2006
attribute year bcodmo_name String year
attribute year description String 4-digit year in YYYY format.
attribute year long_name String Year
attribute year nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/YEARXXXX/ (external link)
attribute year units String unitless
variable microcosm   String  
attribute microcosm bcodmo_name String sample
attribute microcosm description String Identifier of the microcosm. C0 and E0 are the time zero (initial) samples that represent the starting chl-a concentrations in the seawater.
attribute microcosm long_name String Microcosm
attribute microcosm nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/ACYC/ (external link)
attribute microcosm units String unitless
variable microcosm_type   String  
attribute microcosm_type bcodmo_name String sample_descrip
attribute microcosm_type description String Type of microcosm: experimental or control.
attribute microcosm_type long_name String Microcosm Type
attribute microcosm_type units String text
variable Fv_to_Fm_mean   float  
attribute Fv_to_Fm_mean _FillValue float NaN
attribute Fv_to_Fm_mean actual_range float 0.238, 0.617
attribute Fv_to_Fm_mean bcodmo_name String unknown
attribute Fv_to_Fm_mean description String Mean of Fv_to_Fm measured in each microcosm each day.
attribute Fv_to_Fm_mean long_name String Fv To Fm Mean
attribute Fv_to_Fm_mean units String dimensionless
variable Fv_to_Fm_sd   float  
attribute Fv_to_Fm_sd _FillValue float NaN
attribute Fv_to_Fm_sd actual_range float 0.018, 0.16
attribute Fv_to_Fm_sd bcodmo_name String unknown
attribute Fv_to_Fm_sd colorBarMaximum double 50.0
attribute Fv_to_Fm_sd colorBarMinimum double 0.0
attribute Fv_to_Fm_sd description String Standard deviation of Fv_to_Fm_mean.
attribute Fv_to_Fm_sd long_name String Fv To Fm Sd
attribute Fv_to_Fm_sd units String dimensionless
variable comments   String  
attribute comments bcodmo_name String comment
attribute comments description String Free-text notes/comments.
attribute comments long_name String Comments
attribute comments units String text
variable Fv_to_Fm   float  
attribute Fv_to_Fm _FillValue float NaN
attribute Fv_to_Fm actual_range float 0.15, 0.637
attribute Fv_to_Fm bcodmo_name String unknown
attribute Fv_to_Fm description String Ratio of Fv to Fm; indicates the proportion of the maximum possible chlorophyll fluorescence.
attribute Fv_to_Fm long_name String Fv To Fm
attribute Fv_to_Fm units String dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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