ERDDAP > info > bcodmo_dataset_3962

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Results of laboratory experiment examining growth, CO2- and N2-fixation of Crocosphaera watsonii isolates in differing light intensities; conducted in the Hutchins Laboratory, USC		I   M	background			BCO-DMO	bcodmo_dataset_3962

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Culturing and experimental conditions Stock cultures of the two Atlantic C. watsonii isolates were provided courtesy of Dr. Eric Webb. Both isolates were collected in March 2002, WH0401 from 6\u00ba 58.78' N, 49\u00ba 19.70' W and WH0402 from 11\u00ba 42.12S', 32\u00ba 00.64'W. Triplicate cultures were grown using a semi-continuous culturing technique (Garcia et al., 2011) at 28 degrees C in an artificial seawater medium (Chen et al., 1996). Nutrients were added to autoclaved seawater at the concentrations listed in the AQUIL recipe (Morel et al., 1979), except for nitrate, which was omitted. The growth rates of cultures were measured over 2\u20133 day intervals and were used to determine the dilution rate. Culture cell density was kept low (cells ml\u20131 = 50\u2013500 \u00d7 103 for experiments with WH0401 and 5.0\u201330 \u00d7 103 for WH0402) to prevent light limitation of photosynthesis and deviation from the expected pH values for respective pCO2 culture treatments. Light was supplied with cool-white fluorescent lamps on a 12:12 h light:dark cycle and measured with a LI-250A light meter (LiCor Biosciences, light sensor serial# SPQA 4020). Because of large differences in cell size between WH0401 and WH0402, WH0401 was cultured at higher cell densities to maintain relatively equivalent levels of total culture biomass (0.1\u20132.5 mM particulate C for cultures of WH0401; 0.1\u20131.3 mM particulate C for WH0402). Cells were considered fully acclimated to treatment conditions after cultures had remained at steady-state growth for seven generations or more (unless stated otherwise). Fast growing cultures (i.e. high light cultures) were acclimated for more than ten generations while slow growing cultures (i.e. low light and low pCO2 cultures) were acclimated over two months but for fewer generations. Cultures were sampled over the period between 24 and 48 h after the preceding dilution to measure growth rates, gross and net 15N2-fixation rates, CO2-fixation rates, and particulate elemental composition. Light experiments In order to quantify differences in growth and in the CO2- and N2-fixation rate capacities of these two isolates of C. watsonii, the investigators measured growth, CO2-fixation and gross and net N2-fixation rates, and particulate carbon and nitrogen composition in response to a range of light intensities. Growth rate and cell density estimates Growth rate was determined as an increase in culture cell density over time with the equation NT=N0e\u00b5T, where N0 and NT are the initial and final culture cell densities, respectively, T is the time in days between culture cell density estimates, and \u00b5 is the specific growth rate. Culture cell density was determined using a haemocytometer and an Olympus BX51 microscope. Cell diameter was measured using an ocular micrometer calibrated with the same microscope. Growth rates were fitted to a Monod linear hyperbolic function of light (Monod, 1949) using Sigma Plot 10 software program. The hyperbola was fit to the data without including the origin to yield the highest r2 value. N2 fixation The acetylene reduction assay described by Capone et al. (1993) was used to estimate the gross N2-fixation rate. Rate measurements were initiated at the beginning of the 12-h dark period, when C. watsonii is known to fix N2 (Mohr et al., 2010a; Saito et al., 2011). Gross N2-fixation rates were calculated in the same way as described in Garcia et al. (2011), using a Bunsen coefficient for ethylene of 0.082 (Breitbarth et al., 2004) and an ethylene production:N2-fixation ratio of 3:1. Net N2-fixation rates were measured using the 15N2 isotope tracer method (Mulholland & Bernhardt, 2005; Mulholland et al., 2004). Samples were prepared the same way as described in Garcia et al. (2011). Briefly, 169 ml of each experimental replicate was inoculated with 169 \u00b5l of 99% doubly labelled 15N2 gas and incubated at 28 degrees C in complete darkness for 12 h during the dark period. The incubation was then terminated by filtering the entire volume onto precombusted (450 degree C, 4 h) GF/F filters for the analysis of particulate 15N, total particulate N, and total particulate C. Filters were dried at 80\u201390 degrees C, pelleted, and combusted in a quartz column with chromium oxide and silver wool at 1000 degrees C. For this analysis, ammonium sulphate and sucrose were used as standards. At the time the experiments were conducted, the investigators were not aware of the criticisms of the 15N2 uptake method that have been discussed by Mohr et al. (2010b). Thus, for another independent estimate of net N2 fixation, the investigators calculated a particulate N (PN) accumulation rate in cultures over time (deltaPN = PNfinal - PNinitial). Particulate N was measured in subsamples of experimental replicates that were incubated with 15N2 at the end of the dark period and used as the end-period PN measurement (PNfinal). Because only one sample of PN was collected, the investigators back-calculated an estimate of PNinitial based on their measurements of cellular growth rate using the equation: growth rate (d\u20131) = [ln(PNfinal)\u2013ln(PNinitial)]/(t2\u2013t1), where t1 is the initial time and t2 is the final time. Based on their measurements of growth rates, the investigators assumed that PN per cell was in a daily steady state. The gross N2-fixation rate:PN-accumulation rate ratio (hereafter the gross:PN accumulation ratio) was then calculated and compared to the ratio of gross N2-fixation rate:net 15N2-fixation rate ratio (gross:net), which is a proxy for cellular N retention (Mulholland et al., 2004; Mulholland, 2007). CO2 fixation The rate of CO2 fixation was determined as described in Garcia et al. (2011) using the H14CO3- incorporation method. CO2-fixation rates were determined by first calculating the ratio of the radioactivity of 14C incorporated into cells during 24 hours to the total radioactivity of H14CO3\u2013. This ratio was then multiplied by the total CO2 concentration (TCO2). TCO2 concentrations were measured in the CO2-light experiments and were applied to all experiments to calculate CO2-fixation rates for corresponding CO2 treatments. For the light experiments, the investigators used a TCO2 value that was measured in the present-day pCO2 treatments of the CO2-light experiments (2053 \u00b5M TCO2). References: BREITBARTH, E., MILLS, M.M., FRIEDRICHS, G. & LAROCHE, J. (2004). The Bunsen gas solubility coefficient of ethylene as a function of temperature and salinity and its importance for nitrogen fixation assays. Limnology and Oceanography: Methods, 2: 282\u2013288. DOI: [10.4319/lom.2004.2.282](\\"https://dx.doi.org/10.4319/lom.2004.2.282\\") CHEN, Y.B., ZEHR, J.P. & MELLON, M. (1996). Growth and nitrogen fixation of the diazotrophic filamentous nonheterocystous cyanobacterium Trichodesmium sp. IMS101 in defined media: Evidence for a circadian rhythm. Journal of Phycology, 32: 916-923. DOI: [10.1111/j.0022-3646.1996.00916.x](\\"https://dx.doi.org/10.1111/j.0022-3646.1996.00916.x\\") Garcia, N. S., F.-X. Fu, , C. L. Breene, P. W. Bernhardt, M. R. Mulholland, J. A. Sohm, and D. A. Hutchins. 2011. Interactive effects of irradiance and CO2 on CO2- and N2 fixation in the diazotroph Trichodesmium erythraeum (Cyanobacteria). J. Phycol. 47: 1292-1303. DOI:\u00a0[10.1111/j.1529-8817.2011.01078.x](\\"https://dx.doi.org/10.1111/j.1529-8817.2011.01078.x\\") MONOD, J. (1949). The growth of bacterial cultures. Annual Review of Microbiology, 3: 371\u2013394. Morel, F. M. M., J. G. Rueter, D. M. Anderson, and Guillard, R. R. L. 1979. Aquil: Chemically defined phytoplankton culture medium for trace metal studies. J. Phycol. 15:135-141. MULHOLLAND, M.R. (2007). The fate of nitrogen fixed by diazotrophs in the ocean. Biogeosciences 4: 37\u201351. DOI: [10.5194/bg-4-37-2007](\\"https://dx.doi.org/10.5194/bg-4-37-2007\\") MULHOLLAND, M.R. & BERNHARDT, P.W. (2005). The effect of growth rate, phosphorus concentration and temperature on N2-fixation, carbon fixation, and nitrogen release in continuous cultures of Trichodesmium IMS101. Limnology and Oceanography, 50: 839\u2013849. DOI: [10.4319/lo.2005.50.3.0839](\\"https://dx.doi.org/10.4319/lo.2005.50.3.0839\\") MULHOLLAND, M.R., BRONK, D.A. & CAPONE, D.G. (2004). N2 fixation and regeneration of NH4+ and dissolved organic N by Trichodesmium IMS101. Aquatic Microbial Ecology, 37: 85\u201394. DOI: [10.3354/ame037085](\\"https://dx.doi.org/10.3354/ame037085\\")
attribute	NC_GLOBAL	awards_0_award_nid	String	55189
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-0722337
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0722337
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	C. watsonii light experiments (Data published in Fig. 1 of Garcia et al. 2013 Eur. J. Phycology) PI: David Hutchins (USC) Contact: Fei-Xue Fu (USC) Version: 11 June 2013
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2013-06-10T18:40:44Z
attribute	NC_GLOBAL	date_modified	String	2019-11-06T17:18:54Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.3962.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/3962
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Light Meter
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	During culturing, light was measured with a LI-250A light meter (LI-COR Biosciences, light sensor serial # SPQA 4020).
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	6180
attribute	NC_GLOBAL	instruments_0_description	String	Light meters are instruments that measure light intensity. Common units of measure for light intensity are umol/m2/s or uE/m2/s (micromoles per meter squared per second or microEinsteins per meter squared per second). (example: LI-COR 250A)
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Light Meter
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	703
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Light Meter
attribute	NC_GLOBAL	instruments_1_acronym	String	Hemocytometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Culture cell density was determined using a haemocytometer and an Olympus BX51 microscope.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	6181
attribute	NC_GLOBAL	instruments_1_description	String	A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Hemocytometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	704
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Hemocytometer
attribute	NC_GLOBAL	keywords	String	bco, bco-dmo, biological, C_specific_CO2_fix, C_specific_CO2_fix_sd, carbon, carbon dioxide, cell, cell_diameter, cell_diameter_sd, chemical, co2, data, dataset, diameter, dioxide, dmo, erddap, fix, gross, gross_to_net_N2fix, gross_to_net_N2fix_sd, growth, growth_rate, growth_rate_sd, isolate, light, management, n2fix, N_specific_gross_N2_fix, N_specific_gross_N2_fix_sd, N_specific_net_15N2_fix, N_specific_net_15N2_fix_sd, net, oceanography, office, preliminary, rate, specific
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/3962/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/3962
attribute	NC_GLOBAL	param_mapping	String	{'3962': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/3962/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of Southern California
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	USC
attribute	NC_GLOBAL	people_0_person_name	String	David A. Hutchins
attribute	NC_GLOBAL	people_0_person_nid	String	51048
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of Southern California
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	USC
attribute	NC_GLOBAL	people_1_person_name	String	Fei-Xue Fu
attribute	NC_GLOBAL	people_1_person_nid	String	51585
attribute	NC_GLOBAL	people_1_role	String	Contact
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	people_2_affiliation	String	University of Southern California
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	USC
attribute	NC_GLOBAL	people_2_person_name	String	David A. Hutchins
attribute	NC_GLOBAL	people_2_person_nid	String	51048
attribute	NC_GLOBAL	people_2_role	String	Contact
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	people_3_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_3_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_3_person_nid	String	51498
attribute	NC_GLOBAL	people_3_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	project	String	Diaz N2-Fix in High CO2
attribute	NC_GLOBAL	projects_0_acronym	String	Diaz N2-Fix in High CO2
attribute	NC_GLOBAL	projects_0_description	String	From NSF award abstract: The importance of marine N2 fixation to present ocean productivity and global nutrient and carbon biogeochemistry is now universally recognized. Marine N2 fixation rates and oceanic N inventories are also thought to have varied over geological time due to climate variability and change. However, almost nothing is known about the responses of dominant N2 fixers in the ocean such as Trichodesmium and unicellular N2 fixing cyanobacteria to past, present and future global atmospheric CO2 regimes. Our preliminary data demonstrate that N2 and CO2 fixation rates, growth rates, and elemental ratios of Atlantic and Pacific Trichodesmium isolates are controlled by the ambient CO2 concentration at which they are grown. At projected year 2100 pCO2 (750 ppm), N2 fixation rates of both strains increased 35-100%, with simultaneous increases in C fixation rates and cellular N:P and C:P ratios. Surprisingly, these increases in N2 and C fixation due to elevated CO2 were of similar relative magnitude regardless of the growth temperature or P availability. Thus, the influence of CO2 appears to be independent of other common growth-limiting factors. Equally important, Trichodesmium growth and N2 fixation were completely halted at low pCO2 levels (150 ppm), suggesting that diazotrophy by this genus may have been marginal at best at last glacial maximum pCO2 levels of ~190 ppm. Genetic evidence indicates that Trichodesmium diazotrophy is subject to CO2 control because this cyanobacterium lacks high-affinity dissolved inorganic carbon transport capabilities. These findings may force a re-evaluation of the hypothesized role of past marine N2 fixation in glacial/interglacial climate changes, as well as consideration of the potential for increased ocean diazotrophy and altered nutrient and carbon cycling in the future high-CO2 ocean. We propose an interdisciplinary project to examine the relationship between ocean N2 fixing cyanobacteria and changing pCO2. A combined field and laboratory approach will incorporate in situ measurements with experimental manipulations using natural and cultured populations of Trichodesmium and unicellular N2 fixers over range of pCO2 spanning glacial era to future concentrations (150-1500 ppm). We will also examine how effects of pCO2 on N2 and C fixation and elemental stoichiometry are moderated by the availability of other potentially growth-limiting variables such as Fe, P, temperature, and light. We plan to obtain a detailed picture of the full range of responses of important oceanic diazotrophs to changing pCO2, including growth rates, N2 and CO2 fixation, cellular elemental ratios, fixed N release, photosynthetic physiology, and expression of key genes involved in carbon and nitrogen acquisition at both the transcript and protein level. This research has the potential to evolutionize our understanding of controls on N2 fixation in the ocean. Many of our current ideas about the interactions between oceanic N2 fixation, atmospheric CO2, nutrient biogeochemistry, ocean productivity, and global climate change may need revision to take into account previously unrecognized feedback mechanisms between atmospheric composition and diazotrophs. Our findings could thus have major implications for human society, and its increasing dependence on ocean resources in an uncertain future. This project will take the first vital steps towards understanding how a biogeochemically-critical process, the fixation of N2 in the ocean, may respond to our rapidly changing world during the century to come.
attribute	NC_GLOBAL	projects_0_end_date	String	2012-12
attribute	NC_GLOBAL	projects_0_geolocation	String	Laboratory
attribute	NC_GLOBAL	projects_0_name	String	CO2 control of oceanic nitrogen fixation and carbon flow through diazotrophs
attribute	NC_GLOBAL	projects_0_project_nid	String	2172
attribute	NC_GLOBAL	projects_0_start_date	String	2007-05
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Results of laboratory experiment examining growth, CO2- and N2-fixation of Crocosphaera watsonii isolates in differing light intensities; conducted in the Hutchins Laboratory, USC.
attribute	NC_GLOBAL	title	String	Results of laboratory experiment examining growth, CO2- and N2-fixation of Crocosphaera watsonii isolates in differing light intensities; conducted in the Hutchins Laboratory, USC
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	isolate		String
attribute	isolate	bcodmo_name	String	taxon
attribute	isolate	description	String	Name of Crocosphaera watsonii isolate.
attribute	isolate	long_name	String	Isolate
attribute	isolate	units	String	text
variable	light		short
attribute	light	_FillValue	short	32767
attribute	light	actual_range	short	18, 300
attribute	light	bcodmo_name	String	irradiance
attribute	light	description	String	Light intensity. (For more about light measurement see: Australian National Algae Culture Collection and Plant Physiology Online.)
attribute	light	long_name	String	Light
attribute	light	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/VSRW/
attribute	light	units	String	micromoles quanta per square meter per second (umol quanta m-2 s-1)
variable	growth_rate		float
attribute	growth_rate	_FillValue	float	NaN
attribute	growth_rate	actual_range	float	0.0762, 0.8141
attribute	growth_rate	bcodmo_name	String	unknown
attribute	growth_rate	description	String	Growth rate.
attribute	growth_rate	long_name	String	Growth Rate
attribute	growth_rate	units	String	per day
variable	growth_rate_sd		float
attribute	growth_rate_sd	_FillValue	float	NaN
attribute	growth_rate_sd	actual_range	float	0.00301, 0.0379
attribute	growth_rate_sd	bcodmo_name	String	standard deviation
attribute	growth_rate_sd	colorBarMaximum	double	50.0
attribute	growth_rate_sd	colorBarMinimum	double	0.0
attribute	growth_rate_sd	description	String	Standard deviation of growth rate.
attribute	growth_rate_sd	long_name	String	Growth Rate Sd
attribute	growth_rate_sd	units	String	per day
variable	cell_diameter		float
attribute	cell_diameter	_FillValue	float	NaN
attribute	cell_diameter	actual_range	float	2.28, 5.7317
attribute	cell_diameter	bcodmo_name	String	unknown
attribute	cell_diameter	description	String	Cell diamter in micrometers.
attribute	cell_diameter	long_name	String	Cell Diameter
attribute	cell_diameter	units	String	micrometers (um)
variable	cell_diameter_sd		float
attribute	cell_diameter_sd	_FillValue	float	NaN
attribute	cell_diameter_sd	actual_range	float	0.0438, 0.1932
attribute	cell_diameter_sd	bcodmo_name	String	standard deviation
attribute	cell_diameter_sd	colorBarMaximum	double	50.0
attribute	cell_diameter_sd	colorBarMinimum	double	0.0
attribute	cell_diameter_sd	description	String	Standard deviation of cell diameter.
attribute	cell_diameter_sd	long_name	String	Cell Diameter Sd
attribute	cell_diameter_sd	units	String	micrometers (um)
variable	C_specific_CO2_fix		float
attribute	C_specific_CO2_fix	_FillValue	float	NaN
attribute	C_specific_CO2_fix	actual_range	float	8.03E-4, 0.0242
attribute	C_specific_CO2_fix	bcodmo_name	String	unknown
attribute	C_specific_CO2_fix	description	String	C-specific CO2 fixation.
attribute	C_specific_CO2_fix	long_name	String	C Specific CO2 Fix
attribute	C_specific_CO2_fix	units	String	per hour
variable	C_specific_CO2_fix_sd		float
attribute	C_specific_CO2_fix_sd	_FillValue	float	NaN
attribute	C_specific_CO2_fix_sd	actual_range	float	4.19E-5, 0.00344
attribute	C_specific_CO2_fix_sd	bcodmo_name	String	standard deviation
attribute	C_specific_CO2_fix_sd	colorBarMaximum	double	50.0
attribute	C_specific_CO2_fix_sd	colorBarMinimum	double	0.0
attribute	C_specific_CO2_fix_sd	description	String	Standard deviation of C-specific CO2 fixation.
attribute	C_specific_CO2_fix_sd	long_name	String	C Specific CO2 Fix Sd
attribute	C_specific_CO2_fix_sd	units	String	per hour
variable	N_specific_gross_N2_fix		float
attribute	N_specific_gross_N2_fix	_FillValue	float	NaN
attribute	N_specific_gross_N2_fix	actual_range	float	0.00973, 0.0451
attribute	N_specific_gross_N2_fix	bcodmo_name	String	unknown
attribute	N_specific_gross_N2_fix	description	String	N-specific gross N2 fixation.
attribute	N_specific_gross_N2_fix	long_name	String	N Specific Gross N2 Fix
attribute	N_specific_gross_N2_fix	units	String	per hour
variable	N_specific_gross_N2_fix_sd		float
attribute	N_specific_gross_N2_fix_sd	_FillValue	float	NaN
attribute	N_specific_gross_N2_fix_sd	actual_range	float	8.61E-5, 0.00527
attribute	N_specific_gross_N2_fix_sd	bcodmo_name	String	standard deviation
attribute	N_specific_gross_N2_fix_sd	colorBarMaximum	double	50.0
attribute	N_specific_gross_N2_fix_sd	colorBarMinimum	double	0.0
attribute	N_specific_gross_N2_fix_sd	description	String	Standard deviation of N-specific gross N2 fixation.
attribute	N_specific_gross_N2_fix_sd	long_name	String	N Specific Gross N2 Fix Sd
attribute	N_specific_gross_N2_fix_sd	units	String	per hour
variable	N_specific_net_15N2_fix		float
attribute	N_specific_net_15N2_fix	_FillValue	float	NaN
attribute	N_specific_net_15N2_fix	actual_range	float	8.84E-6, 0.0169
attribute	N_specific_net_15N2_fix	bcodmo_name	String	unknown
attribute	N_specific_net_15N2_fix	description	String	N-specific net 15N2 fixation.
attribute	N_specific_net_15N2_fix	long_name	String	N Specific Net 15 N2 Fix
attribute	N_specific_net_15N2_fix	units	String	per hour
variable	N_specific_net_15N2_fix_sd		float
attribute	N_specific_net_15N2_fix_sd	_FillValue	float	NaN
attribute	N_specific_net_15N2_fix_sd	actual_range	float	4.13E-6, 0.00219
attribute	N_specific_net_15N2_fix_sd	bcodmo_name	String	standard deviation
attribute	N_specific_net_15N2_fix_sd	colorBarMaximum	double	50.0
attribute	N_specific_net_15N2_fix_sd	colorBarMinimum	double	0.0
attribute	N_specific_net_15N2_fix_sd	description	String	Standard deviation of N-specific net N2 fixation.
attribute	N_specific_net_15N2_fix_sd	long_name	String	N Specific Net 15 N2 Fix Sd
attribute	N_specific_net_15N2_fix_sd	units	String	per hour
variable	gross_to_net_N2fix		float
attribute	gross_to_net_N2fix	_FillValue	float	NaN
attribute	gross_to_net_N2fix	actual_range	float	2.3955, 15.7753
attribute	gross_to_net_N2fix	bcodmo_name	String	unknown
attribute	gross_to_net_N2fix	description	String	Ratio of gross N2 fixation to net 15N2 fixation.
attribute	gross_to_net_N2fix	long_name	String	Gross To Net N2fix
attribute	gross_to_net_N2fix	units	String	ratio
variable	gross_to_net_N2fix_sd		float
attribute	gross_to_net_N2fix_sd	_FillValue	float	NaN
attribute	gross_to_net_N2fix_sd	actual_range	float	0.075, 2.1655
attribute	gross_to_net_N2fix_sd	bcodmo_name	String	standard deviation
attribute	gross_to_net_N2fix_sd	colorBarMaximum	double	50.0
attribute	gross_to_net_N2fix_sd	colorBarMinimum	double	0.0
attribute	gross_to_net_N2fix_sd	description	String	Standard deviation of the ratio of gross N2 fixation to net 15N2 fixation.
attribute	gross_to_net_N2fix_sd	long_name	String	Gross To Net N2fix Sd
attribute	gross_to_net_N2fix_sd	units	String	ratio

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.