ERDDAP > info > bcodmo_dataset_472032

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	3A: Removal of organic carbon by natural bacterioplankton communities as a function of pCO2 from laboratory experiments between 2012 and 2016		I   M	background			BCO-DMO	bcodmo_dataset_472032

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute	NC_GLOBAL	acquisition_description	String	TOC measurements: The procedures used to set up each experiment (inoculum filtration and dilution with 0.2 um filtrate) removed the majority of particulate organic carbon such that changes in bacterioplankton carbon production and DOC removal were mainly a function of the growth of the inoculum. Ideally, samples collected for organic carbon would be filtered in order to directly assess DOC removal separate from bacterioplankton carbon production over the course of the incubations. However, sample handling during filtration can result in contamination that obscures changes in DOC on the scale of a few micro-molar C. To avoid contamination, seawater samples from the incubation experiments were not filtered. Therefore, measured values of organic carbon include both DOC and bacterioplankton carbon and are considered total organic carbon (TOC). TOC samples were collected into 60 mL high-density polyethylene bottles (Sargasso Sea and South Pacific Subtropical Gyre) or in combusted 40 mL glass EPA vials with Teflon coated silicone septa (Santa Barbara Channel). All TOC samples were frozen at -20 C until analysis. Samples were analyzed via high temperature combustion method on a modified Shimadzu TOC-V or Shimadzu TOC-L using the standardization and referencing approaches described in Carlson et al. 2010. Bacterioplankton abundance measurement \u2013 Samples for bacterioplankton abundance were analyzed by epifluorescence microscopy with 0, 6-diamidino -2-phenyl dihydrochloride (5ug/mL, DAPI, SIGMA-Aldrich, St. Louis, MO, USA) according to Porter and Feig 1980, or by Flow Cytometry (FCM) on an LSR II with SYBR Green I according to Nelson et al. 2011. See Parsons et al. 2014 and Nelson et al. 2011 regarding sample preparation and instrument settings for epifluorescence microscopy and FCM analyses, respectively. DAPI direct counts and FCM analysis enumerate total prokaryotic abundance. We were not able to differentiate between bacterial and archaeal domains and refer to the combined cell densities as bacterioplankton abundance (Glockner et al. 1999). Water sources: Experiment OA11 was conducted on board a research cruise R/V Kilo Moana KM1416. The Sargasso Sea experiments were conducted at the Bermuda Institute for Ocean Sciences (BIOS) with water was collected via the R/V Atlantic Explorer. The Santa Barbara Channel experiments were conducted with water collected near-shore via a pier near the UCSB campus. Experimental design: At all three study sites, experiments consisted of 0.2 um-filtered (0.2 um GSWP, Millipore, Billerica, MA) seawater or 0.2 um-filtered phytoplankton exudate that was inoculated with natural bacterial communities. The inoculum of natural bacterial communities consisted of either unfiltered whole seawater (Sargasso Sea and South Pacific Subtropical Gyre experiments) or 1.2 um filtrate (Santa Barbara Channel experiments; 1.2 um RAWP, Millipore, Billerica, MA). Particulate organic carbon concentration in oligotrophic gyres is low (1-3 umol L-1) so to avoid filtration artifacts such as reduced bacterial production (unpublished data) and contamination of DOC due to handling, the inoculum was not pre-filtered for the experiments conducted in oligotrophic waters. Because particulate organic carbon concentration can be much greater in coastal upwelling systems it was necessary to remove large particles and organisms from the inoculum. Inoculum was added at 25 \u2013 30% of final volume, effectively diluting grazer concentrations and grazing pressure. All filters were pre-rinsed with ~2 L of deionized distilled water and sample water prior to use in order to remove organic contaminants from the filters. Four types of DOC treatments were used and are described in the data as \"doc_additions\": 1\. None: unamended seawater, which provided naturally occurring DOC. 2. CNP: Naturally occurring DOC amended with glucose (~10 uM C) plus NH4 Cl (1uM) and K2HPO4 (0.1uM) (CNP) 3. Species name + \" exudate\": phytoplankton exudate 4. Species name + \" lysate\": naturally occurring DOC amended with phytoplankton lysate (~10 uM C L-1; labeled by phytoplankton species used). The various treatments were generated by inoculating the 0.2 um pre-filtered seawater or exudate with the microbial community; this solution was then divided into two polycarbonate (PC) containers to adjust\u00a0pCO2.\u00a0pCO2 levels were adjusted via chemical additions (Sargasso Sea experiment) or by bubbling with CO2-mixed air (Santa Barbara Channel and South Pacific Subtropical Gyre experiments). Adjusted seawater incubations were then transferred into new PC carboys and CNP or lysate was added, if appropriate. A very small volume of lysate (1.2 mL to 11.5 L of experimental volume) or CNP (12 mL to 10 L of experimental water for the Sargasso Sea experiment; 0.28 mL to 10 L of experimental volume for the Santa Barbara Channel experiment) was added to minimize perturbing the carbonate chemistry. All experiments were conducted in duplicate, at in situ temperatures, and in the dark to eliminate photoautotrophic production. All PC bottles had been acid-washed (5 % or 10 % HCL) and rinsed with deionized distilled water and sample water before use. \u00a0
attribute	NC_GLOBAL	awards_0_award_nid	String	55209
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1041038
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward?AWD_ID=1041038&HistoricalAwards=false
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	Donald L. Rice
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	51467
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Bacterial use of DOC as a function of pCO2 Uta Passow, PI version 28 Nov 2016
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2013-11-25T16:22:02Z
attribute	NC_GLOBAL	date_modified	String	2019-09-04T18:22:42Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.665253
attribute	NC_GLOBAL	Easternmost_Easting	double	-64.6353
attribute	NC_GLOBAL	geospatial_lat_max	double	34.4216
attribute	NC_GLOBAL	geospatial_lat_min	double	-17.4502
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-64.6353
attribute	NC_GLOBAL	geospatial_lon_min	double	-149.8727
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/472032
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Shimadzu TOC-V
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Samples were analyzed via high-temperature combustion method on a modified Shimadzu TOC-V or Shimadzu TOC-L using the standardization and referencing approaches described in Carlson et al. 2010.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	665756
attribute	NC_GLOBAL	instruments_0_description	String	A Shimadzu TOC-V Analyzer measures DOC by high temperature combustion method.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	http://onto.nerc.ac.uk/CAST/124
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Shimadzu TOC-V Analyzer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	603
attribute	NC_GLOBAL	instruments_0_supplied_name	String	modified Shimadzu TOC-L
attribute	NC_GLOBAL	instruments_1_acronym	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Flow Cytometry (FCM) on an LSR II with SYBR Green I according to Nelson et al. 2011.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	665758
attribute	NC_GLOBAL	instruments_1_description	String	Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB37/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	660
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Flow Cytometry (FCM)
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Samples for bacterioplankton abundance were analyzed by epifluorescence microscopy with 0, 6-diamidino -2-phenyl dihydrochloride (5µg mL-1, DAPI, SIGMA-Aldrich, St. Louis, MO, USA) according to Porter and Feig 1980.
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	665757
attribute	NC_GLOBAL	instruments_2_description	String	Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB06/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Microscope-Fluorescence
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	695
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Epifluorescence microscopy
attribute	NC_GLOBAL	instruments_3_acronym	String	Shimadzu TOC-L
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Samples were analyzed via high-temperature combustion method on a modified Shimadzu TOC-V or Shimadzu TOC-L using the standardization and referencing approaches described in Carlson et al. 2010.
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	665755
attribute	NC_GLOBAL	instruments_3_description	String	A Shimadzu TOC-L Analyzer measures DOC by high temperature combustion method. Developed by Shimadzu, the 680 degree C combustion catalytic oxidation method is now used worldwide. One of its most important features is the capacity to efficiently oxidize hard-to-decompose organic compounds, including insoluble and macromolecular organic compounds. The 680 degree C combustion catalytic oxidation method has been adopted for the TOC-L series. http://www.shimadzu.com/an/toc/lab/toc-l2.html
attribute	NC_GLOBAL	instruments_3_instrument_external_identifier	String	http://onto.nerc.ac.uk/CAST/124.html
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Shimadzu TOC-L Analyzer
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	527277
attribute	NC_GLOBAL	instruments_3_supplied_name	String	modified Shimazdu TOC-L
attribute	NC_GLOBAL	keywords	String	abun, addition, average, bact, bact_abun_x10e6_avg, bact_abun_x10e6_stderr, bact_abun_x10e6_stdev, bco, bco-dmo, biological, bottle, bottle_number, carbon, carbon dioxide, chemical, co2, commerce, data, dataset, date, days, department, deviation, dioxide, dmo, doc, doc_addition, erddap, experiment, latitude, longitude, management, number, oceanography, office, point, preliminary, site, standard, standard deviation, stderr, stdev, target, target_pCO2, time, time_days, time_point, toc, toc_avg, toc_stderr, toc_stdev, x10e6
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/472032/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/472032
attribute	NC_GLOBAL	Northernmost_Northing	double	34.4216
attribute	NC_GLOBAL	param_mapping	String	{'472032': {'latitude': 'flag - latitude', 'longitude': 'flag - longitude'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/472032/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of California-Santa Barbara
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UCSB-MSI
attribute	NC_GLOBAL	people_0_person_name	String	Dr Uta Passow
attribute	NC_GLOBAL	people_0_person_nid	String	51317
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of California-Santa Barbara
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	UCSB-MSI
attribute	NC_GLOBAL	people_1_person_name	String	Mark A. Brzezinski
attribute	NC_GLOBAL	people_1_person_nid	String	50663
attribute	NC_GLOBAL	people_1_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_1_role_type	String	originator
attribute	NC_GLOBAL	people_2_affiliation	String	University of California-Santa Barbara
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	UCSB-MSI
attribute	NC_GLOBAL	people_2_person_name	String	Craig Carlson
attribute	NC_GLOBAL	people_2_person_nid	String	50575
attribute	NC_GLOBAL	people_2_role	String	Co-Principal Investigator
attribute	NC_GLOBAL	people_2_role_type	String	originator
attribute	NC_GLOBAL	people_3_affiliation	String	University of California-Santa Barbara
attribute	NC_GLOBAL	people_3_affiliation_acronym	String	UCSB-MSI
attribute	NC_GLOBAL	people_3_person_name	String	Ms Anna James
attribute	NC_GLOBAL	people_3_person_nid	String	471722
attribute	NC_GLOBAL	people_3_role	String	Student
attribute	NC_GLOBAL	people_3_role_type	String	related
attribute	NC_GLOBAL	people_4_affiliation	String	University of California-Santa Barbara
attribute	NC_GLOBAL	people_4_affiliation_acronym	String	UCSB-MSI
attribute	NC_GLOBAL	people_4_person_name	String	Dr Uta Passow
attribute	NC_GLOBAL	people_4_person_nid	String	51317
attribute	NC_GLOBAL	people_4_role	String	Contact
attribute	NC_GLOBAL	people_4_role_type	String	related
attribute	NC_GLOBAL	people_5_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_5_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_5_person_name	String	Stephen R. Gegg
attribute	NC_GLOBAL	people_5_person_nid	String	50910
attribute	NC_GLOBAL	people_5_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_5_role_type	String	related
attribute	NC_GLOBAL	people_6_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_6_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_6_person_name	String	Amber York
attribute	NC_GLOBAL	people_6_person_nid	String	643627
attribute	NC_GLOBAL	people_6_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_6_role_type	String	related
attribute	NC_GLOBAL	project	String	OA - Effects of High CO2
attribute	NC_GLOBAL	projects_0_acronym	String	OA - Effects of High CO2
attribute	NC_GLOBAL	projects_0_description	String	From the NSF Award Abstract Coastal waters are already experiencing episodic exposure to carbonate conditions that were not expected until the end of the century making understanding the response to these episodic events as important as understanding the long-term mean response. Among the most striking examples are those associated with coastal upwelling along the west coast of the US, where the pH of surface waters may drop to 7.6 and pCO2 can reach 1100 uatm. Upwelling systems are responsible for a significant fraction of global carbon export making them prime targets for investigations on how ocean acidification is already affecting the biological pump today. In this study, researchers at the University of California at Santa Barbara will investigate the potential effects of ocean acidification on the strength of the biological pump under the transient increases in CO2 experienced due to upwelling. Increases in CO2 are expected to alter the path and processing of carbon through marine food webs thereby strengthening the biological pump. Increases in inorganic carbon without proportional increases in nutrients result in carbon over-consumption by phytoplankton. How carbon over-consumption affects the strength of the biological pump will depend on the fate of the extra carbon that is either incorporated into phytoplankton cells forming particulate organic matter (POM), or is excreted as dissolved organic matter (DOM). Results from mesocosm experiments demonstrate that the mechanisms controlling the partitioning of fixed carbon between the particulate and dissolved phases, and the processing of those materials, are obscured when both processes operate simultaneously under natural or semi-natural conditions. Here, POM and DOM production and the heterotrophic processing of these materials will be separated experimentally across a range of CO2 concentrations by conducting basic laboratory culture experiments. In this way the mechanisms whereby elevated CO2 alters the flow of carbon along these paths can be elucidated and better understood for use in mechanistic forecasting models. Broader Impacts- The need to understand the effects of ocean acidification for the future of society is clear. In addition to research education, both formal and informal, will be important for informing the public. Within this project 1-2 graduate students and 2-3 minority students will be recruited as interns from the CAMP program (California Alliance for Minority Participation). Within the 'Ocean to Classrooms' program run by outreach personnel from UCSB's Marine Science Institute an educational unit for K-12 students will be developed. Advice and support is also given to the Education Coordinator of NOAA, Channel Islands National Marine Sanctuary for the development of an education unit on ocean acidification. PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH Arnosti C, Grossart H-P, Muehling M, Joint I, Passow U. "Dynamics of extracellular enzyme activities in seawater under changed atmsopheric pCO2: A mesocosm investigation.," Aquatic Microbial Ecology, v.64, 2011, p. 285. Passow U. "The Abiotic Formation of TEP under Ocean Acidification Scenarios.," Marine Chemistry, v.128-129, 2011, p. 72. Passow, Uta; Carlson, Craig A.. "The biological pump in a high CO2 world," MARINE ECOLOGY PROGRESS SERIES, v.470, 2012, p. 249-271. Gaerdes, Astrid; Ramaye, Yannic; Grossart, Hans-Peter; Passow, Uta; Ullrich, Matthias S.. "Effects of Marinobacter adhaerens HP15 on polymer exudation by Thalassiosira weissflogii at different N:P ratios," MARINE ECOLOGY PROGRESS SERIES, v.461, 2012, p. 1-14. Philip Boyd, Tatiana Rynearson, Evelyn Armstrong, Feixue Fu, Kendra Hayashi, Zhangi Hu, David Hutchins, Raphe Kudela, Elena Litchman, Margaret Mulholland, Uta Passow, Robert Strzepek, Kerry Whittaker, Elizabeth Yu, Mridul Thomas. "Marine Phytoplankton Temperature versus Growth Responses from Polar to Tropical Waters - Outcome of a Scientific Community-Wide Study," PLOS One 8, v.8, 2013, p. e63091. Arnosti, C., B. M. Fuchs, R. Amann, and U. Passow. "Contrasting extracellular enzyme activities of particle-associated bacteria from distinct provinces of the North Atlantic Ocean," Frontiers in Microbiology, v.3, 2012, p. 1. Koch, B.P., Kattner, G., Witt, M., Passow, U., 2014. Molecular insights into the microbial formation of marine dissolved organic matter: recalcitrant or labile? Biogeosciences Discuss. 11 (2), 3065-3111. Taucher, J., Brzezinski, M., Carlson, C., James, A., Jones, J., Passow, U., Riebesell, U., submitted. Effects of warming and elevated pCO2 on carbon uptake and partitioning of the marine diatoms Thalassiosira weissflogii and Dactyliosolen fragilissimus. Limnology and Oceanography
attribute	NC_GLOBAL	projects_0_end_date	String	2014-09
attribute	NC_GLOBAL	projects_0_geolocation	String	Passow Lab, Marine Science Institute, University of California Santa Barbara
attribute	NC_GLOBAL	projects_0_name	String	Will high CO2 conditions affect production, partitioning and fate of organic matter?
attribute	NC_GLOBAL	projects_0_project_nid	String	2284
attribute	NC_GLOBAL	projects_0_project_website	String	http://www.msi.ucsb.edu/people/research-scientists/uta-passow
attribute	NC_GLOBAL	projects_0_start_date	String	2010-10
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	-17.4502
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Factors that affect the removal of organic carbon by heterotrophic bacterioplankton can impact the rate and magnitude of organic carbon loss in the ocean through the conversion of a portion of consumed organic carbon to CO2. Through enhanced rates of consumption, surface bacterioplankton communities can also reduce the amount of dissolved organic carbon (DOC) available for export from the surface ocean. The present study investigated the direct effects of elevated pCO2 on bacterioplankton removal of several forms of DOC ranging from glucose to complex phytoplankton exudate and lysate, and naturally occurring DOC. Elevated pCO2 (1000 \u2013 1500 ppm) enhanced both the rate and magnitude of organic carbon removal by bacterioplankton communities compared to low (pre-industrial and ambient) pCO2 (250 \u2013 ~400 ppm). The increased removal was largely due to enhanced respiration, rather than enhanced production of bacterioplankton biomass.
attribute	NC_GLOBAL	title	String	3A: Removal of organic carbon by natural bacterioplankton communities as a function of pCO2 from laboratory experiments between 2012 and 2016
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-149.8727
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	experiment		String
attribute	experiment	bcodmo_name	String	exp_id
attribute	experiment	description	String	Experiment identifier
attribute	experiment	long_name	String	Experiment
attribute	experiment	units	String	unitless
variable	site		String
attribute	site	bcodmo_name	String	site
attribute	site	description	String	Site the water for the experiment came from
attribute	site	long_name	String	Site
attribute	site	units	String	unitless
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	-17.4502, 34.4216
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	Latitude where water samples were collected; north is positive.
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-149.8727, -64.6353
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	Longitude where water samples were collected; west is negative.
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	bottle_number		String
attribute	bottle_number	bcodmo_name	String	bottle
attribute	bottle_number	description	String	Bottle identifier
attribute	bottle_number	long_name	String	Bottle Number
attribute	bottle_number	units	String	unitless
variable	doc_addition		String
attribute	doc_addition	bcodmo_name	String	treatment
attribute	doc_addition	description	String	Dissolved organic carbon additions. See Aquisition Description section for an explaination of values.
attribute	doc_addition	long_name	String	Doc Addition
attribute	doc_addition	units	String	unitless
variable	target_pCO2		short
attribute	target_pCO2	_FillValue	short	32767
attribute	target_pCO2	actual_range	short	250, 1500
attribute	target_pCO2	bcodmo_name	String	pCO2
attribute	target_pCO2	description	String	Target pCO2 level
attribute	target_pCO2	long_name	String	Target P CO2
attribute	target_pCO2	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/PCO2C101/
attribute	target_pCO2	units	String	parts per million (ppm)
variable	time_point		String
attribute	time_point	bcodmo_name	String	time_point
attribute	time_point	description	String	Time point identifier in experiment
attribute	time_point	long_name	String	Time Point
attribute	time_point	units	String	unitless
variable	time_days		float
attribute	time_days	_FillValue	float	NaN
attribute	time_days	actual_range	float	0.0, 17.05
attribute	time_days	bcodmo_name	String	time_elapsed
attribute	time_days	description	String	Elapsed time since start of experiment in days
attribute	time_days	long_name	String	Time Days
attribute	time_days	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ELTMZZZZ/
attribute	time_days	units	String	unitless
variable	date		String
attribute	date	bcodmo_name	String	date
attribute	date	description	String	Date of experiment in format YYYY-MM-DD
attribute	date	long_name	String	Date
attribute	date	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	date	units	String	unitless
variable	bact_abun_x10e6_avg		float
attribute	bact_abun_x10e6_avg	_FillValue	float	NaN
attribute	bact_abun_x10e6_avg	actual_range	float	0.0, 4.86
attribute	bact_abun_x10e6_avg	bcodmo_name	String	bact_abundance
attribute	bact_abun_x10e6_avg	description	String	Bacterial abundance multiplied by 10^6
attribute	bact_abun_x10e6_avg	long_name	String	Bact Abun X10e6 Avg
attribute	bact_abun_x10e6_avg	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/BNTX
attribute	bact_abun_x10e6_avg	units	String	cells per milliliter
variable	bact_abun_x10e6_stderr		float
attribute	bact_abun_x10e6_stderr	_FillValue	float	NaN
attribute	bact_abun_x10e6_stderr	actual_range	float	0.0, 1.16
attribute	bact_abun_x10e6_stderr	bcodmo_name	String	bact_abundance
attribute	bact_abun_x10e6_stderr	colorBarMaximum	double	50.0
attribute	bact_abun_x10e6_stderr	colorBarMinimum	double	0.0
attribute	bact_abun_x10e6_stderr	description	String	Standard error of bacterial abundance multiplied by 10^6
attribute	bact_abun_x10e6_stderr	long_name	String	Bact Abun X10e6 Stderr
attribute	bact_abun_x10e6_stderr	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/BNTX
attribute	bact_abun_x10e6_stderr	units	String	cells per milliliter
variable	bact_abun_x10e6_stdev		float
attribute	bact_abun_x10e6_stdev	_FillValue	float	NaN
attribute	bact_abun_x10e6_stdev	actual_range	float	0.0, 1.64
attribute	bact_abun_x10e6_stdev	bcodmo_name	String	bact_abundance
attribute	bact_abun_x10e6_stdev	description	String	Standard deviation Bacterial abundance multiplied by 10^6
attribute	bact_abun_x10e6_stdev	long_name	String	Bact Abun X10e6 Stdev
attribute	bact_abun_x10e6_stdev	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/BNTX
attribute	bact_abun_x10e6_stdev	units	String	cells per milliliter
variable	toc_avg		float
attribute	toc_avg	_FillValue	float	NaN
attribute	toc_avg	actual_range	float	63.84, 398.62
attribute	toc_avg	bcodmo_name	String	TOC
attribute	toc_avg	description	String	Total organic carbon
attribute	toc_avg	long_name	String	Toc Avg
attribute	toc_avg	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CORGCOTX/
attribute	toc_avg	units	String	micromoles per liter (uM)
variable	toc_stderr		float
attribute	toc_stderr	_FillValue	float	NaN
attribute	toc_stderr	actual_range	float	0.0, 14.2
attribute	toc_stderr	bcodmo_name	String	TOC
attribute	toc_stderr	colorBarMaximum	double	50.0
attribute	toc_stderr	colorBarMinimum	double	0.0
attribute	toc_stderr	description	String	Standard error of total organic carbon
attribute	toc_stderr	long_name	String	Toc Stderr
attribute	toc_stderr	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CORGCOTX/
attribute	toc_stderr	units	String	micromoles per liter (uM)
variable	toc_stdev		float
attribute	toc_stdev	_FillValue	float	NaN
attribute	toc_stdev	actual_range	float	0.0, 20.08
attribute	toc_stdev	bcodmo_name	String	TOC
attribute	toc_stdev	description	String	Standard deviation of total organic carbon
attribute	toc_stdev	long_name	String	Toc Stdev
attribute	toc_stdev	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CORGCOTX/
attribute	toc_stdev	units	String	micromoles per liter (uM)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.