ERDDAP > info > bcodmo_dataset_489382

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Results from experiment examining effects of 4 different dyes on growth rates of scleractinian corals; from the Cohen lab at WHOI in Woods Hole, MA (OA Nutrition and Coral Calcification project)		I   M	background			BCO-DMO	bcodmo_dataset_489382

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Methodology as described in Holcomb et al. (2013): Colonies of the temperate scleractinian coral Astrangia poculata were collected and processed as previously described. Newly settled polyps and their associated substratum were attached to slides. The slides with corals were suspended vertically in a flow-through aquarium receiving 20 micrometers filtered Vineyard Sound seawater. Corals experienced a temperature range of 14 to 30 degrees C. Aquaria were aerated, and corals were maintained under these conditions for at least one month prior to use in experiments. A mixture of brown and white colonies (zooxanthellate and azooxanthellate colonies) was used for all treatments. For the marking experiments, corals were placed in pre-washed containers with lids containing ~800 ml of water from the source aquarium. Airstones were added to each container and each container bubbled continuously. Containers were held within a water bath with a temperature similar to that of the source aquarium. One of four dyes was used to mark the coral skeleton: alizarin red S (sodium salt \u2013 Alfa Aesar 42040 lot E22R017 \u2013 referred to as alizarin), alizarin complexone (Alfa Aesar A16699 lot E8180A), calcein (Alfa Aesar L10255 lot USLF006789), and oxytetracycline HCl (USB 23659 lot 113648). The dye experiments took place from March to October 2009. Growth rates were estimated via alkalinity depletion measurements the day before (pre- treatment), the day of (treatment), and the day after (post-treatment) dye exposure. The alkalinity incubations were about 24 hours in duration (one full light-dark cycle). Temperatures ranged from 25 to 26 degrees C. Four to seven corals were used in each treatment, each in a separate incubation container. Irradiance in each container, measured using a diving-PAM underwater quantum sensor (WALZ), ranged from 10 to 40 micromoles photons/m^2/sec. Samples for alkalinity were taken from each container about 1 hour after the corals were added, and once again at the end of the incubation. Salinity and pH were also measured at the end of each incubation for each container (and at the start for a sub-set of the containers). Aragonite deposition was assumed to be the only process affecting alkalinity, with 2 mol alkalinity consumed per mol of CaCO3 deposited. Alkalinity depletion rates were corrected for evaporation and background rates measured in the control containers (the control containers contained no slides). Alkalinity was measured via titration with 0.01 N HCl containing 40.7 g NaCl/l using a Metrohm Titrando 808 Dosimat and a 730 Sample Changer controlled by Tiamo software to perform automated normalized Gran titrations of 1 ml samples.
attribute	NC_GLOBAL	awards_0_award_nid	String	54896
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-1041106
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1041106
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Coral growth (species <i>Astrangia poculata</i>) dye experiments PI: Anne Cohen (WHOI) Contact: Michael Holcomb Version: 31 Jan 2014
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2014-01-31T20:25:18Z
attribute	NC_GLOBAL	date_modified	String	2019-11-14T19:52:03Z
attribute	NC_GLOBAL	defaultDataQuery	String	&amp;time&lt;now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.489382.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/489382
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Benchtop pH Meter
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	A Thermo-Orion ROSS 8165BNWP electrode, read to 0.1 mV, was used to measure the pH of each experimental container.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	489403
attribute	NC_GLOBAL	instruments_0_description	String	An instrument consisting of an electronic voltmeter and pH-responsive electrode that gives a direct conversion of voltage differences to differences of pH at the measurement temperature. (McGraw-Hill Dictionary of Scientific and Technical Terms) This instrument does not map to the NERC instrument vocabulary term for 'pH Sensor' which measures values in the water column. Benchtop models are typically employed for stationary lab applications.
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Benchtop pH Meter
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	681
attribute	NC_GLOBAL	instruments_0_supplied_name	String	pH Probe
attribute	NC_GLOBAL	instruments_1_acronym	String	Automatic titrator
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Alkalinities were measured via titration with 0.01 N HCl containing 40.7 g NaCl/l using a Metrohm Titrando 808 Dosimat and a 730 Sample Changer controlled by Tiamo software to performautomated normalized Gran titrations of 1 ml samples.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	489405
attribute	NC_GLOBAL	instruments_1_description	String	Instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB12/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Automatic titrator
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	682
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Titrator
attribute	NC_GLOBAL	instruments_2_acronym	String	Aquarium
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	489463
attribute	NC_GLOBAL	instruments_2_description	String	Aquarium - a vivarium consisting of at least one transparent side in which water-dwelling plants or animals are kept
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Aquarium
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	711
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Aquarium
attribute	NC_GLOBAL	instruments_3_acronym	String	Conductivity Meter
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	A Hach conductivity probe (read to 0.1, accurate to ~1) was used to determine the salinity of each experimental container.
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	489402
attribute	NC_GLOBAL	instruments_3_description	String	Conductivity Meter - An electrical conductivity meter (EC meter) measures the electrical conductivity in a solution. Commonly used in hydroponics, aquaculture and freshwater systems to monitor the amount of nutrients, salts or impurities in the water.
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Conductivity Meter
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	719
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Conductivity Probe
attribute	NC_GLOBAL	keywords	String	abbrev, bco, bco-dmo, biological, chemical, data, dataset, dmo, during, erddap, growth, management, oceanography, office, post, preliminary, rel, rel_growth_during_treatment, rel_growth_post_treatment, treatment, treatment_abbrev
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/489382/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/489382
attribute	NC_GLOBAL	param_mapping	String	{'489382': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/489382/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	WHOI
attribute	NC_GLOBAL	people_0_person_name	String	Anne L Cohen
attribute	NC_GLOBAL	people_0_person_nid	String	51428
attribute	NC_GLOBAL	people_0_role	String	Lead Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI
attribute	NC_GLOBAL	people_1_person_name	String	Michael Holcomb
attribute	NC_GLOBAL	people_1_person_nid	String	489381
attribute	NC_GLOBAL	people_1_role	String	Contact
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	people_2_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_2_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_2_person_nid	String	51498
attribute	NC_GLOBAL	people_2_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	project	String	OA Nutrition and Coral Calcification
attribute	NC_GLOBAL	projects_0_acronym	String	OA Nutrition and Coral Calcification
attribute	NC_GLOBAL	projects_0_description	String	The project description is a modification of the original NSF award abstract. This research project is part of the larger NSF funded CRI-OA collaborative research initiative and was funded as an Ocean Acidification-Category 1, 2010 award. Over the course of this century, all tropical coral reef ecosystems, whether fringing heavily populated coastlines or lining remote islands and atolls, face unprecedented threat from ocean acidification caused by rising levels of atmospheric CO2. In many laboratory experiments conducted to date, calcium carbonate production (calcification) by scleractinian (stony) corals showed an inverse correlation to seawater saturation state OMEGAar), whether OMEGAar was manipulated by acid or CO2 addition. Based on these data, it is predicted that coral calcification rates could decline by up to 80% of modern values by the end of this century. A growing body of new experimental data however, suggests that the coral calcification response to ocean acidification may be less straightforward and a lot more variable than previously recognized. In at least 10 recent experiments including our own, 8 different tropical and temperate species reared under nutritionally-replete but significantly elevated CO2 conditions (780-1200 ppm, OMEAGar ~1.5-2), continued to calcify at rates comparable to conspecifics reared under ambient CO2. These experimental results are consistent with initial field data collected on reefs in the eastern Pacific and southern Oman, where corals today live and accrete their skeletons under conditions equivalent to 2X and 3X pre-industrial CO2. On these high CO2, high nutrient reefs (where nitrate concentrations typically exceed 2.5 micro-molar), coral growth rates rival, and sometimes even exceed, those of conspecifics in low CO2, oligotrophic reef environments. The investigators propose that a coral's energetic status, tightly coupled to the availability of inorganic nutrients and/or food, is a key factor in the calcification response to CO2-induced ocean acidification. Their hypothesis, if confirmed by the proposed laboratory investigations, implies that predicted changes in coastal and open ocean nutrient concentrations over the course of this century, driven by both climate impacts on ocean stratification and by increased human activity in coastal regions, could play a critical role in exacerbating and in some areas, modulating the coral reef response to ocean acidification. This research program builds on the investigators initial results and observations. The planned laboratory experiments will test the hypothesis that: (1) The coral calcification response to ocean acidification is linked to the energetic status of the coral host. The relative contribution of symbiont photosynthesis and heterotrophic feeding to a coral's energetic status varies amongst species. Enhancing the energetic status of corals reared under high CO2, either by stimulating photosynthesis with inorganic nutrients or by direct heterotrophic feeding of the host lowers the sensitivity of calcification to decreased seawater OMEGAar; (2) A species-specific threshold CO2 level exists over which enhanced energetic status can no longer compensate for decreased OMEGAar of the external seawater. Similarly, we will test the hypothesis that a nutrient threshold exists over which nutrients become detrimental for calcification even under high CO2 conditions; and (3) Temperature-induced reduction of algal symbionts is one stressor that can reduce the energetic reserve of the coral host and exacerbate the calcification response to ocean acidification. The investigator's initial findings highlight the critical importance of energetic status in the coral calcification response to ocean acidification. Verification of these findings in the laboratory, and identification of nutrient and CO2 thresholds for a range of species will have immediate, direct impact on predictions of reef resilience in a high CO2 world. The research project brings together a diverse group of expertise in coral biogeochemistry, chemical oceanography, molecular biology and coral reproductive ecology to focus on a problem that has enormous societal, economic and conservation relevance.
attribute	NC_GLOBAL	projects_0_end_date	String	2013-09
attribute	NC_GLOBAL	projects_0_geolocation	String	global; experimental
attribute	NC_GLOBAL	projects_0_name	String	An Investigation of the Role of Nutrition in the Coral Calcification Response to Ocean Acidification
attribute	NC_GLOBAL	projects_0_project_nid	String	2183
attribute	NC_GLOBAL	projects_0_start_date	String	2010-10
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Results from experiment examining effects of 4 different dyes on growth rates of scleractinian corals; from the Cohen lab at WHOI in Woods Hole, MA.
attribute	NC_GLOBAL	title	String	Results from experiment examining effects of 4 different dyes on growth rates of scleractinian corals; from the Cohen lab at WHOI in Woods Hole, MA (OA Nutrition and Coral Calcification project)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	treatment		String
attribute	treatment	bcodmo_name	String	treatment
attribute	treatment	description	String	Name of the dye used to mark the coral.
attribute	treatment	long_name	String	Treatment
attribute	treatment	units	String	dimensionless
variable	treatment_abbrev		String
attribute	treatment_abbrev	bcodmo_name	String	treatment
attribute	treatment_abbrev	description	String	Abbreviation used to identify the treatment type in Holcomb et al. 2013.
attribute	treatment_abbrev	long_name	String	Treatment Abbrev
attribute	treatment_abbrev	units	String	dimensionless
variable	rel_growth_during_treatment		double
attribute	rel_growth_during_treatment	_FillValue	double	NaN
attribute	rel_growth_during_treatment	actual_range	double	0.517823968, 1.381243035
attribute	rel_growth_during_treatment	bcodmo_name	String	unknown
attribute	rel_growth_during_treatment	description	String	Relative coral growth rate estimated via alkalinity depletion measurements the day of dye exposure.
attribute	rel_growth_during_treatment	long_name	String	Rel Growth During Treatment
attribute	rel_growth_during_treatment	units	String	dimensionless
variable	rel_growth_post_treatment		double
attribute	rel_growth_post_treatment	_FillValue	double	NaN
attribute	rel_growth_post_treatment	actual_range	double	0.737756892, 1.79697093
attribute	rel_growth_post_treatment	bcodmo_name	String	unknown
attribute	rel_growth_post_treatment	description	String	Relative coral growth rate estimated via alkalinity depletion measurements the day after dye exposure.
attribute	rel_growth_post_treatment	long_name	String	Rel Growth Post Treatment
attribute	rel_growth_post_treatment	units	String	dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.