ERDDAP > info > bcodmo_dataset_506135

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
	set	data	graph		files	public	[Thalassiosira wessiflogii growth rate and TEP] - Results from growth rate experiment with the diatom Thalassiosira wessiflogii in semi-continuous culture; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project) (Effect of Temperature on Extracellular Polymeric Substance Production (EPS) by Diatoms)		I   M	background			BCO-DMO	bcodmo_dataset_506135

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Growth of the diatom Thalassiosira wessiflogii (CCMP 1051) was obtained from the National Center for Culture of Marine Algae and Microbiota (NCMA). The diatom was grown in artificial seawater (Berges et al. 2001) in nitrogen-limited 1000 ml semi- continuous cultures at a sequence of dilution rates. The macronutrient concentrations in the artificial seawater recipe were modified from Berges et al. (2001) to affect nitrogen limitation; concentrations of nitrogen, phosphorus and silicon were 60 \u00b5M (as NaNO3), 100 \u00b5M (NaH2PO4), and 100 \u00b5M (Na2SiO3), respectively. Culture temperature was maintained at 20 \u00b1 0.1 \u00b0C throughout the experiment. Photon flux density on the surface of the culture bottles was 150 \u00b5mol m-2 s-1. The cultures were stirred with 2.5 cm long stir bars using magnetic stirrers at 120 revolutions per minute. The cultures were grown at a sequence of dilution rates (0.3, 0.5, 0.7, 0.9 and 0.3 day-1) affected by daily dilution at 10:00 am every day. To induce a dilution rate of 0.3 day-1, 0.3 of the culture volume (300 ml) was removed and replaced with 300 ml of fresh medium to maintain a constant total culture volume (1000 ml). Measures of phytoplankton abundance and biomass Counts of 400 cells from each replicate culture were made by light microscopy using a hemocytometer (Fuchs-Rosenthal ruling, Hauser Scientific) (Guillard and Sieracki 2005) from samples preserved in Lugol\u2019s iodine (Parsons et al. 1984). Cell volume was determined using live cells (Menden- Deuer and Lessard 2000). The volume of 100 diatoms from each replicate culture was determined by measuring cell length (pervalver length) and width (valver length) at 400x magnification using a light microscope (Axioplan 2, Carl Zeiss MicroImaging). Cell volume was calculated based on the assumption that T. wessiflogii is a cylinder. Chlorophyll a concentrations in the cultures was determined by fluorescence (Arar and Collins 1997). Chlorophyll a concentration 90% acetone extractions from biomass retained on GF/C (Whatman) were measured using a Turner Designs 700 fluorometer, which was calibrated using chlorophyll a standards (Sigma) (Arar and Collins 1997). The extract was diluted with 90% acetone if the chl. a concentration were too high. The carbon and nitrogen content of particulate organic matter in the cultures was determined by elemental analysis using a Carlo Erba NA1500 Elemental Analyzer. Standards were acetanilide, methionine, graphite (USGS 24, USGS 40, and USGS 41) (Verardo et al. 1990).\u00a0 Bacteria abundance Bacteria (400 cells) were counted using an epifluorescence microscope (Axioplan 2, Carl Zeiss MicroImaging) after staining with 4'6-diamidino-2-phenylindole dihydrochloride (DAPI) (Porter and Feig 1980) at a final concentration of 0.25 \u00b5g ml-1. Cell permeability Uptake and staining with the membrane-impermeable SYTOX Green (Invitrogen) was used to determine what proportion of the diatom population had permeable cell membranes (Veldhuis et al. 2001, Franklin et al. 2012). Four hundred cells were examined using an epifluorescence microscope and the number of cells that stained with SYTOX Green was enumerated. Total carbohydrate Total carbohydrate concentrations were determined in unfiltered liquid samples from the cultures using the phenol-sulfuric acid (PSA) method (Dubois et al. 1956) calibrated with d-glucose. The concentration of total carbohydrate was expressed as glucose equivalents. TEP staining and analysis Transparent exopolymer particles (TEP) were sampled according to Alldredge et al. (1993) and TEP abundance was enumerated by image analysis (Logan et al. 1994, Engel 2009). Ten photomicrographs were taken of each slide and the area of 100 TEP particles from each replicate culture was determined after manually drawing around each particle using Axio Vision 4.8 (Carl Zeiss MicroImaging ) image analysis software.\u00a0 Particle size distribution and aggregation The particle size distribution (PSD) and volume concentration of particles in the T. weissiflogii cultures was measured using laser scattering following the method of Rzadkowolski and Thornton (2012) using a Laser In Situ Scattering and Transmissometry instrument (LISST-100X, Type C; Sequoia Scientific). Sample (150 ml) from each replicate culture was placed into a chamber attached to the LISST and the PSD was measured 100 times at a rate of 1 Hz. The PSD of the culture was blank corrected by subtracting the PSD of 0.2 \u00b5m filtered artificial seawater. References cited Alldredge, A. L., Passow, U. & Logan B. E. 1993. The abundance and significance of a class of large, transparent organic particles in the ocean. Deep-Sea Res. Oceanogr., I. 40: 1131-1140. doi:[10.1016/0967-0637(93)90129-Q](\\"https://dx.doi.org/10.1016/0967-0637\(93\)90129-Q\\") Arar, E. J. & Collins, G. B. 1997. Method 445.0. In Vitro Determination of Chlorophyll a and Pheophytin a in Marine and Freshwater Algae by Fluorescence U.S. Environmental Protection Agency, Cincinnati, Ohio. Berges, J. A., Franklin D. J. & Harrison, P. J. 2001. Evolution of an artificial seawater medium: Improvements in enriched seawater, artificial water over the last two decades. J. Phycol. 37:1138-1145. doi:[10.1046/j.1529-8817.2001.01052.x](\\"https://dx.doi.org/10.1046/j.1529-8817.2001.01052.x\\") Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350\u2013356. doi:[10.1021/ac60111a017](\\"https://dx.doi.org/10.1021/ac60111a017\\") Franklin, D. J., Airs, R. L., Fernandes, M., Bell, T. G., Bongaerts, R. J., Berges, J. A. & Malin, G. 2012. Identification of senescence and death in Emiliania huxleyi and Thalassiosira pseudonana: Cell staining, chlorophyll alterations, and dimethylsulfoniopropionate (DMSP) metabolism. Limnol. Oceanogr. 57: 305\u2013317. doi:10.4319/lo.2012.57.1.0305 Guillard, R. R. L. & Sieracki, M. S. 2005. Counting cells in cultures with the light microscope. In Andersen R. A. [Ed.] Algal Culturing Techniques. Elsevier Academic Press, Burlington, MA, pp. 239-252. Logan, B. E., Grossart, H. P. & Simon, M. 1994. Direct observation of phytoplankton, TEP and aggregates on polycarbonate filters using brightfield microscopy. J. Plankton Res.16: 1811-1815.doi:[10.1093/plankt/16.12.1811](\\"https://dx.doi.org/10.1093/plankt/16.12.1811\\") Menden-Deuer S. & Lessard, E. J. 2000. Carbon to volume relationships for dinoflagellates, diatoms, and other protists plankton. Limnol. Oceanogr. 45: 569- 579. doi:[10.4319/lo.2000.45.3.0569](\\"https://dx.doi.org/10.4319/lo.2000.45.3.0569\\") Parsons, T. R., Maita, Y. & Lalli, C. M. 1984. A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, Oxford, UK. Passow, U. & Alldredge, A. L. 1995. A dye-binding assay for the spectrophotometric measurement of transparent exopolymer particles (TEP). Limnol. Oceanogr. 40: 1326-1335. doi:[10.4319/lo.1995.40.7.1326](\\"https://dx.doi.org/10.4319/lo.1995.40.7.1326\\") Porter, K. G. & Feig, Y. S. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25:943\u2013948. doi:[10.4319/lo.1980.25.5.0943](\\"https://dx.doi.org/10.4319/lo.1980.25.5.0943\\") Rzadkowlski, C. E. & Thornton, D. C. O. 2012. Using laser scattering to identify diatoms and conduct aggregation experiments. Eur. J. Phycol.47:30-41. doi:[10.1080/09670262.2011.646314](\\"https://dx.doi.org/10.1080/09670262.2011.646314\\") Veldhuis, M. J. W., Kraay, G. W. & Timmermans, K. R. 2001. Cell death in phytoplankton: correlation between changes in membrane permeability, photosynthetic activity, pigmentation and growth. Eur. J. Phycol. 36: 167\u2013177. doi:[10.1080/09670260110001735318](\\"https://dx.doi.org/10.1080/09670260110001735318\\") Verardo, D. J., Froelich, P. N. & McIntyre, A. 1990. Determination of organic carbon and nitrogen in marine sediments using the Carlo Erba NA-1500 analyzer. Deep-Sea Res.A 37:157-165. doi:[10.1016/0198-0149(90)90034-S](\\"https://dx.doi.org/10.1016/0198-0149\(90\)90034-S\\")
attribute	NC_GLOBAL	awards_0_award_nid	String	55158
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-0726369
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0726369
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Thalassiosira wessiflogii growth rate and TEP PI: Daniel C.O. Thornton (Texas A&M) Version: 07 April 2014
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2014-04-07T16:01:42Z
attribute	NC_GLOBAL	date_modified	String	2019-11-21T17:48:58Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.506135.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/506135
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	TD-700
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Chlorophyll a concentration 90% acetone extractions from biomass retained on GF/C (Whatman) were measured using a Turner Designs 700 fluorometer, which was calibrated using chlorophyll a standards (Sigma) (Arar and Collins 1997).
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	506238
attribute	NC_GLOBAL	instruments_0_description	String	The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0510/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Turner Designs 700 Laboratory Fluorometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	694
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Turner Designs 700 Fluorometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Bacterial abunance and cell permeability were determined using an epifluorescence microscope (Axioplan 2, Carl Zeiss MicroImaging).
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	506241
attribute	NC_GLOBAL	instruments_1_description	String	Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB06/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Microscope-Fluorescence
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	695
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Epifluorescence Microscope
attribute	NC_GLOBAL	instruments_2_acronym	String	Hemocytometer
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Counts of 400 cells from each replicate culture were made by light microscopy using a hemocytometer (Fuchs-Rosenthal ruling, Hauser Scientific).
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	506236
attribute	NC_GLOBAL	instruments_2_description	String	A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Hemocytometer
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	704
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Hemocytometer
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	The volume of 100 diatoms from each replicate culture was determined by measuring cell length (pervalver length) and width (valver length) at 400x magnification using a light microscope (Axioplan 2, Carl Zeiss MicroImaging).
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	506237
attribute	NC_GLOBAL	instruments_3_description	String	Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute	NC_GLOBAL	instruments_3_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB05/
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Microscope-Optical
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	708
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Light microscope
attribute	NC_GLOBAL	instruments_4_acronym	String	Carlo-Erba NA-1500
attribute	NC_GLOBAL	instruments_4_dataset_instrument_description	String	The carbon and nitrogen content of particulate organic matter in the cultures was determined by elemental analysis using a Carlo Erba NA1500 Elemental Analyzer.
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	506240
attribute	NC_GLOBAL	instruments_4_description	String	A laboratory instrument that simultaneously determines total nitrogen and total carbon from a wide range of organic and inorganic sediment samples. The sample is completely and instantaneously oxidised by flash combustion, which converts all organic and inorganic substances into combustion products. The resulting combustion gases pass through a reduction furnace and are swept into the chromatographic column by the carrier gas which is helium. The gases are separated in the column and detected by the thermal conductivity detector which gives an output signal proportional to the concentration of the individual components of the mixture. The instrument was originally manufactured by Carlo-Erba, which has since been replaced by Thermo Scientific (part of Thermo Fisher Scientific). This model is no longer in production.
attribute	NC_GLOBAL	instruments_4_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0470/
attribute	NC_GLOBAL	instruments_4_instrument_name	String	Carlo-Erba NA-1500 Elemental Analyzer
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	506239
attribute	NC_GLOBAL	instruments_4_supplied_name	String	Carlo Erba NA1500 Elemental Analyzer
attribute	NC_GLOBAL	instruments_5_acronym	String	Sequoia LISST
attribute	NC_GLOBAL	instruments_5_dataset_instrument_description	String	The particle size distribution (PSD) and volume concentration of particles in the T. weissiflogii cultures was measured using laser scattering following the method of Rzadkowolski and Thornton (2012) using a Laser In Situ Scattering and Transmissometry instrument (LISST-100X, Type C; Sequoia Scientific).
attribute	NC_GLOBAL	instruments_5_dataset_instrument_nid	String	506244
attribute	NC_GLOBAL	instruments_5_description	String	A self-contained unit which measures the scattering of LASER light at a number of angles. This primary measurement is mathematically inverted to give a grain size distribution, and also scaled to obtain the volume scattering function. The size distribution is presented as concentration in each of the grain-size class bins. Optical transmission, water depth and temperature are recorded as supporting measurements. The Sequoia LISST 100-X series instruments are available in two range sizes: 1.25-250 microns (Type B) and 2.5-500 microns (Type C).
attribute	NC_GLOBAL	instruments_5_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0044/
attribute	NC_GLOBAL	instruments_5_instrument_name	String	Sequoia Scientific Laser In-Situ Sediment Size Transmissometer
attribute	NC_GLOBAL	instruments_5_instrument_nid	String	506243
attribute	NC_GLOBAL	instruments_5_supplied_name	String	LISST-100X Type C Sequoia Scientific
attribute	NC_GLOBAL	keywords	String	abundance, agg, agg_vol_conc, agg_vol_conc_n, agg_vol_conc_sd, area, bact, bact_per_diatom, bacteria, bco, bco-dmo, biological, C_to_N, carb, cell, cell_abundance, cell_vol_mean, cell_vol_n, cell_vol_sd, cells, chemical, chemistry, chla, chla_per_cell, chla_per_cell_vol, chlorophyll, chlorophyll-a, conc, concentration, concentration_of_chlorophyll_in_sea_water, culture, data, dataset, day, diatom, dilution, dilution_rate, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Chlorophyll, erddap, management, mean, ocean, oceanography, oceans, office, pcnt, per, preliminary, prod, rate, science, sea, seawater, size, stained, stained_cells, stained_cells_pcnt, tep, TEP_mean_size, TEP_n, TEP_prod_rate, TEP_sd, tot, tot_carb, tot_carb_per_cell, tot_carb_per_cell_vol, tot_TEP_area, vol, vol_conc, vol_conc_n, vol_conc_sd, water
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/506135/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/506135
attribute	NC_GLOBAL	param_mapping	String	{'506135': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/506135/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Texas A&M University
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	TAMU
attribute	NC_GLOBAL	people_0_person_name	String	Daniel C.O. Thornton
attribute	NC_GLOBAL	people_0_person_nid	String	51644
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_1_person_nid	String	51498
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Diatom EPS Production
attribute	NC_GLOBAL	projects_0_acronym	String	Diatom EPS Production
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF Propsoal: It is necessary to determine the fate of organic matter in the ocean to understand marine food webs, biogeochemical cycles, and climate change. Diatoms fix approximately a quarter of the net global primary production each year, and a significant proportion of this production is excreted as extracellular polymeric substances (EPS). EPS have a profound impact on pelagic ecosystems by affecting the formation of aggregates. Diatoms and other particulate organic carbon (POC) sink rapidly as aggregates, affecting the biological carbon pump, which plays a pivotal role in the sequestration of carbon in the ocean. The proposed research will test the central hypothesis: Temperature increase affects diatom release of EPS, which act as a glue, increasing aggregation. Previous work by the investigator showed that increased temperatures affected the aggregation of Skeletonema costatum. Four specific hypotheses will be tested: H1: Diatoms produce more EPS with increasing temperature. H2: Diatoms produce more transparent exopolymer particles (TEP) with increasing temperature. H3: The quantity or composition of cell-surface carbohydrates in diatoms changes with temperature. H4: Aggregation of diatom cultures and natural plankton increases with temperature. Laboratory experiments (years 1 - 2) will be conducted with three model diatom species grown at controlled growth rates and defined limitation (nitrogen or light) in continuous culture. Culture temperature will be stepped up or down in small increments to determine the effect of the temperature change on EPS production, aggregation, and partitioning of carbon in intra- and extracellular pools. Similar experiments in year 3 will be carried out using natural plankton populations from a coastal site where diatoms contribute a significant proportion to the biomass. The proposed research will increase our understanding of the ecology and physiology of one of the dominant groups of primary producers on Earth. EPS are a central aspect of diatom biology, though the physiology, function and broader ecosystem impacts of EPS production remain unknown. This research will determine how temperature, light limitation, and nutrient limitation affect the partitioning of production between dissolved, gel, and particulate phases in the ocean. Measurements of plankton stickiness (alpha) under different conditions will be important to model aggregation processes in the ocean as alpha is an important (and variable) term in coagulation models. Determining how carbon is cycled between the ocean, atmosphere and lithosphere is key to understanding climate change on both geological and human time scales. This is a major societal issue as atmospheric CO2 concentrations are steadily increasing, correlating with a 0.6 C rise in global average temperature during the last century. This research will address potential feedbacks between warming of the surface ocean, diatom ecophysiology and the biological carbon pump. Related Publications: Rzadkowolski, Charles E. and Thornton, Daniel C. O. (2012) Using laser scattering to identify diatoms and conduct aggregation experiments. Eur. J. Phycol., 47(1): 30-41. DOI: 10.1080/09670262.2011.646314 Thornton, Daniel C. O. (2009) Effect of Low pH on Carbohydrate Production by a Marine Planktonic Diatom (Chaetoceros muelleri). Research Letters in Ecology, vol. 2009, Article ID 105901, 4 pages. DOI: 10.1155/2009/105901 Thornton, D.C.O. (2014) Dissolved organic matter (DOM) release by phytoplankton in the contemporary and future ocean. European Journal of Phycology 49: 20-46. DOI: 10.1080/09670262.2013.875596 Thornton, D.C.O., Visser, L.A. (2009) Measurement of acid polysaccharides (APS) associated with microphytobenthos in salt marsh sediments. Aquat Microb Ecol 54:185-198. DOI: 10.3354/ame01265
attribute	NC_GLOBAL	projects_0_end_date	String	2012-08
attribute	NC_GLOBAL	projects_0_geolocation	String	O&M Building, Texas A&M University, College Station, TX 77840
attribute	NC_GLOBAL	projects_0_name	String	Effect of Temperature on Extracellular Polymeric Substance Production (EPS) by Diatoms
attribute	NC_GLOBAL	projects_0_project_nid	String	2255
attribute	NC_GLOBAL	projects_0_start_date	String	2007-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	subsetVariables	String	cell_vol_n,TEP_n,vol_conc_n,agg_vol_conc_n
attribute	NC_GLOBAL	summary	String	Data from laboratory experiment on growth rate and transparent exopolymer particles (TEP) in the diatom Thalassiosira wessiflogii (CCMP 1051) in a semi-continuous culture (four replicate cultures).
attribute	NC_GLOBAL	title	String	[Thalassiosira wessiflogii growth rate and TEP] - Results from growth rate experiment with the diatom Thalassiosira wessiflogii in semi-continuous culture; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project) (Effect of Temperature on Extracellular Polymeric Substance Production (EPS) by Diatoms)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	day		byte
attribute	day	_FillValue	byte	127
attribute	day	actual_range	byte	15, 123
attribute	day	bcodmo_name	String	unknown
attribute	day	description	String	Day of the experiment.
attribute	day	long_name	String	Day
attribute	day	units	String	dimensionless
variable	dilution_rate		String
attribute	dilution_rate	bcodmo_name	String	unknown
attribute	dilution_rate	description	String	Dilution rate.
attribute	dilution_rate	long_name	String	Dilution Rate
attribute	dilution_rate	units	String	per day (day-1)
variable	culture		String
attribute	culture	bcodmo_name	String	replicate
attribute	culture	description	String	Identifier of the culture replicate.
attribute	culture	long_name	String	Culture
attribute	culture	units	String	dimensionless
variable	cell_abundance		int
attribute	cell_abundance	_FillValue	int	2147483647
attribute	cell_abundance	actual_range	int	20100, 146000
attribute	cell_abundance	bcodmo_name	String	diatom
attribute	cell_abundance	description	String	Cell count. Counts of 400 cells were made by transmitted light microscopy using a hemacytometer (Fuchs-Rosenthal ruling Hauser Scientific) (Guillard & Sieracki 2005).
attribute	cell_abundance	long_name	String	Cell Abundance
attribute	cell_abundance	units	String	cells per milliliter
variable	cell_vol_mean		short
attribute	cell_vol_mean	_FillValue	short	32767
attribute	cell_vol_mean	actual_range	short	388, 1348
attribute	cell_vol_mean	bcodmo_name	String	unknown
attribute	cell_vol_mean	description	String	Mean cell volume estimated assuming T. weissflogii (CCMP 1051) was a cyclinder using the method of Menden-Deuer & Lessard (2000).
attribute	cell_vol_mean	long_name	String	Cell Vol Mean
attribute	cell_vol_mean	units	String	cubic micrometers (um^3)
variable	cell_vol_sd		short
attribute	cell_vol_sd	_FillValue	short	32767
attribute	cell_vol_sd	actual_range	short	81, 1176
attribute	cell_vol_sd	bcodmo_name	String	standard deviation
attribute	cell_vol_sd	colorBarMaximum	double	50.0
attribute	cell_vol_sd	colorBarMinimum	double	0.0
attribute	cell_vol_sd	description	String	Standard deviation of cell_vol_mean.
attribute	cell_vol_sd	long_name	String	Cell Vol Sd
attribute	cell_vol_sd	units	String	cubic micrometers (um^3)
variable	cell_vol_n		byte
attribute	cell_vol_n	_FillValue	byte	127
attribute	cell_vol_n	actual_range	byte	25, 25
attribute	cell_vol_n	bcodmo_name	String	number
attribute	cell_vol_n	description	String	n (number of cells) used in determination of cell_vol_mean.
attribute	cell_vol_n	long_name	String	Cell Vol N
attribute	cell_vol_n	units	String	dimensionless
variable	chla		float
attribute	chla	_FillValue	float	NaN
attribute	chla	actual_range	float	12.0, 126.6
attribute	chla	bcodmo_name	String	chlorophyll a
attribute	chla	colorBarMaximum	double	30.0
attribute	chla	colorBarMinimum	double	0.03
attribute	chla	colorBarScale	String	Log
attribute	chla	description	String	Concentration of chlorophyll a measured by fluorescence (Arar & Collins 1997; Method 445.0. EPA).
attribute	chla	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	chla	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/
attribute	chla	units	String	micrograms per liter (ug L-1)
variable	chla_per_cell		float
attribute	chla_per_cell	_FillValue	float	NaN
attribute	chla_per_cell	actual_range	float	0.51, 1.89
attribute	chla_per_cell	bcodmo_name	String	unknown
attribute	chla_per_cell	colorBarMaximum	double	30.0
attribute	chla_per_cell	colorBarMinimum	double	0.03
attribute	chla_per_cell	colorBarScale	String	Log
attribute	chla_per_cell	description	String	Concentration of chlorophyll a per cell.
attribute	chla_per_cell	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	chla_per_cell	units	String	picograms per cell (pg cell-1)
variable	chla_per_cell_vol		float
attribute	chla_per_cell_vol	_FillValue	float	NaN
attribute	chla_per_cell_vol	actual_range	float	0.5, 2.65
attribute	chla_per_cell_vol	bcodmo_name	String	unknown
attribute	chla_per_cell_vol	colorBarMaximum	double	30.0
attribute	chla_per_cell_vol	colorBarMinimum	double	0.03
attribute	chla_per_cell_vol	colorBarScale	String	Log
attribute	chla_per_cell_vol	description	String	Concentration of chlorophyll a per cell volume.
attribute	chla_per_cell_vol	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	chla_per_cell_vol	units	String	femtograms per cubic micrometer (fg um-3)
variable	tot_carb		float
attribute	tot_carb	_FillValue	float	NaN
attribute	tot_carb	actual_range	float	2.47, 13.83
attribute	tot_carb	bcodmo_name	String	unknown
attribute	tot_carb	description	String	Total carbohydrate concentration measured using the PSA method (Dubois et al. 1956).
attribute	tot_carb	long_name	String	Tot Carb
attribute	tot_carb	units	String	micrograms per milliliter (ug mL-1)
variable	tot_carb_per_cell		short
attribute	tot_carb_per_cell	_FillValue	short	32767
attribute	tot_carb_per_cell	actual_range	short	49, 197
attribute	tot_carb_per_cell	bcodmo_name	String	unknown
attribute	tot_carb_per_cell	description	String	Total carbohydrate concentration per cell.
attribute	tot_carb_per_cell	long_name	String	Tot Carb Per Cell
attribute	tot_carb_per_cell	units	String	picograms per cell (pg cell-1)
variable	tot_carb_per_cell_vol		short
attribute	tot_carb_per_cell_vol	_FillValue	short	32767
attribute	tot_carb_per_cell_vol	actual_range	short	57, 237
attribute	tot_carb_per_cell_vol	bcodmo_name	String	unknown
attribute	tot_carb_per_cell_vol	description	String	Total carbohydrate concentration per cell volume.
attribute	tot_carb_per_cell_vol	long_name	String	Tot Carb Per Cell Vol
attribute	tot_carb_per_cell_vol	units	String	femtograms per cubic micrometer (fg um-3)
variable	TEP		int
attribute	TEP	_FillValue	int	2147483647
attribute	TEP	actual_range	int	152800, 504800
attribute	TEP	bcodmo_name	String	unknown
attribute	TEP	description	String	Transparent exopolymer particles (TEP) retained on 0.4 polycarbonate filters and stained with Alcian blue (Alldredge et al. 1993).
attribute	TEP	long_name	String	TEP
attribute	TEP	units	String	TEP per milliliter (TEP mL-1)
variable	TEP_mean_size		short
attribute	TEP_mean_size	_FillValue	short	32767
attribute	TEP_mean_size	actual_range	short	701, 2465
attribute	TEP_mean_size	bcodmo_name	String	unknown
attribute	TEP_mean_size	description	String	Mean size of Transparent exopolymer particles (TEP).
attribute	TEP_mean_size	long_name	String	TEP Mean Size
attribute	TEP_mean_size	units	String	square micrometers (um^2)
variable	TEP_sd		short
attribute	TEP_sd	_FillValue	short	32767
attribute	TEP_sd	actual_range	short	286, 1443
attribute	TEP_sd	bcodmo_name	String	standard deviation
attribute	TEP_sd	colorBarMaximum	double	50.0
attribute	TEP_sd	colorBarMinimum	double	0.0
attribute	TEP_sd	description	String	Standard deviation of TEP_mean_size.
attribute	TEP_sd	long_name	String	TEP Sd
attribute	TEP_sd	units	String	square micrometers (um^2)
variable	TEP_n		byte
attribute	TEP_n	_FillValue	byte	127
attribute	TEP_n	actual_range	byte	25, 25
attribute	TEP_n	bcodmo_name	String	number
attribute	TEP_n	description	String	n used in determination of TEP_mean_size.
attribute	TEP_n	long_name	String	TEP N
attribute	TEP_n	units	String	dimensionless
variable	tot_TEP_area		short
attribute	tot_TEP_area	_FillValue	short	32767
attribute	tot_TEP_area	actual_range	short	231, 604
attribute	tot_TEP_area	bcodmo_name	String	unknown
attribute	tot_TEP_area	description	String	Total TEP area.
attribute	tot_TEP_area	long_name	String	Tot TEP Area
attribute	tot_TEP_area	units	String	square millimeters per milliliter (mm^2 mL-1)
variable	TEP_prod_rate		short
attribute	TEP_prod_rate	_FillValue	short	32767
attribute	TEP_prod_rate	actual_range	short	64, 330
attribute	TEP_prod_rate	bcodmo_name	String	unknown
attribute	TEP_prod_rate	description	String	TEP production rate.
attribute	TEP_prod_rate	long_name	String	TEP Prod Rate
attribute	TEP_prod_rate	units	String	square millimeters per milliliter per day (mm^2 mL-1 day-1)
variable	vol_conc		short
attribute	vol_conc	_FillValue	short	32767
attribute	vol_conc	actual_range	short	49, 393
attribute	vol_conc	bcodmo_name	String	unknown
attribute	vol_conc	description	String	Particulate volume concentration. Volume concentration and aggegation were measured using Laser in situ sacattering and transmissometry (LISST) (Rzadkowolski & Thornton 2012).
attribute	vol_conc	long_name	String	Vol Conc
attribute	vol_conc	units	String	microliters per liter (uL L-1)
variable	vol_conc_sd		short
attribute	vol_conc_sd	_FillValue	short	32767
attribute	vol_conc_sd	actual_range	short	3, 193
attribute	vol_conc_sd	bcodmo_name	String	standard deviation
attribute	vol_conc_sd	colorBarMaximum	double	50.0
attribute	vol_conc_sd	colorBarMinimum	double	0.0
attribute	vol_conc_sd	description	String	Standard deviation of vol_conc.
attribute	vol_conc_sd	long_name	String	Vol Conc Sd
attribute	vol_conc_sd	units	String	microliters per liter (uL L-1)
variable	vol_conc_n		byte
attribute	vol_conc_n	_FillValue	byte	127
attribute	vol_conc_n	actual_range	byte	100, 100
attribute	vol_conc_n	bcodmo_name	String	number
attribute	vol_conc_n	description	String	n used in determination of vol_conc.
attribute	vol_conc_n	long_name	String	Vol Conc N
attribute	vol_conc_n	units	String	dimensionless
variable	agg_vol_conc		short
attribute	agg_vol_conc	_FillValue	short	32767
attribute	agg_vol_conc	actual_range	short	15, 225
attribute	agg_vol_conc	bcodmo_name	String	unknown
attribute	agg_vol_conc	description	String	Aggregated volume concentration (particles > 63 um ESD). Particulate volume concentration and aggegation were measured using Laser in situ sacattering and transmissometry (LISST) (Rzadkowolski & Thornton 2012).
attribute	agg_vol_conc	long_name	String	Agg Vol Conc
attribute	agg_vol_conc	units	String	microliters per liter (uL L-1)
variable	agg_vol_conc_sd		byte
attribute	agg_vol_conc_sd	_FillValue	byte	127
attribute	agg_vol_conc_sd	actual_range	byte	3, 48
attribute	agg_vol_conc_sd	bcodmo_name	String	standard deviation
attribute	agg_vol_conc_sd	colorBarMaximum	double	50.0
attribute	agg_vol_conc_sd	colorBarMinimum	double	0.0
attribute	agg_vol_conc_sd	description	String	Standard deviation of agg_vol_conc.
attribute	agg_vol_conc_sd	long_name	String	Agg Vol Conc Sd
attribute	agg_vol_conc_sd	units	String	microliters per liter (uL L-1)
variable	agg_vol_conc_n		byte
attribute	agg_vol_conc_n	_FillValue	byte	127
attribute	agg_vol_conc_n	actual_range	byte	100, 100
attribute	agg_vol_conc_n	bcodmo_name	String	number
attribute	agg_vol_conc_n	description	String	n used in determination of agg_vol_conc.
attribute	agg_vol_conc_n	long_name	String	Agg Vol Conc N
attribute	agg_vol_conc_n	units	String	dimensionless
variable	stained_cells		short
attribute	stained_cells	_FillValue	short	32767
attribute	stained_cells	actual_range	short	64, 4660
attribute	stained_cells	bcodmo_name	String	unknown
attribute	stained_cells	description	String	Number of SYTOX Green stained cells. Cell permeability was determined by SYTOX Green staining (Veldhuis et al. 1997). Four hundred cells were examined using an epifluorescence microscope and the number of cells that stained with SYTOX Green was enumerated.
attribute	stained_cells	long_name	String	Stained Cells
attribute	stained_cells	units	String	cells per milliliter (cells mL-1)
variable	stained_cells_pcnt		float
attribute	stained_cells_pcnt	_FillValue	float	NaN
attribute	stained_cells_pcnt	actual_range	float	0.2, 3.6
attribute	stained_cells_pcnt	bcodmo_name	String	unknown
attribute	stained_cells_pcnt	description	String	% of SYTOX Green stained cells. Cell permeability was determined by SYTOX Green staining (Veldhuis et al. 1997). Four hundred cells were examined using an epifluorescence microscope and the number of cells that stained with SYTOX Green was enumerated.
attribute	stained_cells_pcnt	long_name	String	Stained Cells Pcnt
attribute	stained_cells_pcnt	units	String	percent (%)
variable	bacteria		int
attribute	bacteria	_FillValue	int	2147483647
attribute	bacteria	actual_range	int	9284, 2668932
attribute	bacteria	bcodmo_name	String	bact_abundance
attribute	bacteria	description	String	Bacteria abundance determined by DAPI staining and counts using an epifluorescence microscope (Porter & Feig 1980).
attribute	bacteria	long_name	String	Bacteria
attribute	bacteria	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/BNTX
attribute	bacteria	units	String	cells per milliliter (cells mL-1)
variable	bact_per_diatom		float
attribute	bact_per_diatom	_FillValue	float	NaN
attribute	bact_per_diatom	actual_range	float	0.3, 21.1
attribute	bact_per_diatom	bcodmo_name	String	unknown
attribute	bact_per_diatom	description	String	Bacteria abundance per diatom.
attribute	bact_per_diatom	long_name	String	Bact Per Diatom
attribute	bact_per_diatom	units	String	dimensionless
variable	C_to_N		float
attribute	C_to_N	_FillValue	float	NaN
attribute	C_to_N	actual_range	float	8.1, 18.8
attribute	C_to_N	bcodmo_name	String	C_to_N
attribute	C_to_N	description	String	Ratio of carbon to nitrogen. C:N ratio was measured using a Carlo Erba NA1500 Elemental Analyzer. Standards were acetanilide, methionine, graphite (USGS 24, USGS 40, and USGS 41) (Verardo, Froelich, & McIntyre 1990).
attribute	C_to_N	long_name	String	C To N
attribute	C_to_N	units	String	dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.