ERDDAP > info > bcodmo_dataset_511217

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[oxidative stress TEP] - Experimental results: Exopolymer production by phytoplankton under oxidative stress; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project) (Effect of Temperature on Extracellular Polymeric Substance Production (EPS) by Diatoms)		I   M	background			BCO-DMO	bcodmo_dataset_511217

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Growth of the phytoplankton The diatom Thalassiosira wessiflogii (CCMP 1051) and the cyanobacterium Synechococcus elongates_cf (CCMP 1379) were obtained from the National Center for Culture of Marine Algae and Microbiota (NCMA). Replicated (n = 3) Batch cultures were grown in artificial seawater (Berges et al. 2001) containing nitrogen, phosphorus and silicon at 400 \u00b5M (as NaNO3), 25 \u00b5M (NaH2PO4), and 400 \u00b5M (Na2SiO3), respectively. Culture temperatures were maintained at 20 \u00b1 1 \u00b0C. Photon flux density on the surface of the culture bottles was 40 to 45 \u00b5mol m-2 s-1 on a 14 hour light: 10 hour dark cycle. During exponential growth, each culture was split into three treatments in which oxidative stress was induced by the addition of hydrogen peroxide at final concentrations of 0 (control), 10 and 100 \u00b5M H2O2. The treatments were sampled once a day over the next three days. Measures of phytoplankton abundance and biomass Counts of 400 cells from each culture were made using hemocytometers (Guillard and Sieracki 2005) from samples preserved in Lugol\u2019s iodine (Parsons et al. 1984) using a light microscope (Axioplan 2, Carl Zeiss MicroImaging). Turbidity of the cultures, used as an indicator of growth, was measured by absorbance at 750 nm in a 1 cm path cuvette using a UV-Mini 1240 spectrophotometer (Shimadzu Corporation). Chlorophyll a concentration 90% acetone extractions from biomass retained on GF/C (Whatman) were measured using a Turner Designs 700 fluorometer, which was calibrated using chlorophyll a standards (Sigma) (Arar and Collins 1997). The extract was diluted with 90% acetone if the chl a concentration were too high. Cell permeability Uptake and staining with the membrane-impermeable SYTOX Green (Invitrogen) was used to determine what proportion of the diatom population had permeable cell membranes (Veldhuis et al. 2001, Franklin et al. 2012). Four hundred cells were examined using an epifluorescence microscope (Axioplan 2, Carl Zeiss MicroImaging) and the number of cells that stained with SYTOX Green was enumerated. TEP staining and analysis Transparent exopolymer particles (TEP) were sampled according to Alldredge et al. (1993) and TEP abundance was enumerated by image analysis (Logan et al. 1994, Engel 2009). Ten photomicrographs were taken of each slide using a light microscope (Axioplan 2, Carl Zeiss MicroImaging). Images were analyzed using ImageJ software (National Institutes of Health) based on the method of Engel (2009). Thresholding during image processing was done using the triangle method (Zack et al. 1977). CSP staining and analysis Coomassie staining particles (CSP) were sampled according to Long and Azam et al. (1996) and CSP abundance was enumerated by image analysis (Logan et al. 1994, Engel 2009). Ten photomicrographs were taken of each slide using a light microscope (Axioplan 2, Carl Zeiss MicroImaging). Images were analyzed using ImageJ software (National Institutes of Health) based on the method of Engel (2009). Thresholding during image processing was done using the triangle method (Zack et al. 1977). Quantum yield of photosystem II The quantum yield of photosystem II was used as an indicator of phytoplankton health and measured using the saturating pulse method (Genty et al. 1989) using a pulse amplitude modulated fluorometer (PAM-210, Heinz Walz GmbH) folowing a protocol based on Marwood et al. (1999). Caspase-like activity Caspase-like activity was measured based on the method of Bouchard & Purdie (2011). Phytoplankton were collected by centrifugation, then lysed in a buffer, and the caspase-3 like activity was measured in the extracted proteins using a Enzcheck Caspase-3 Assay Kit #1 (Invitrogen inc.). The fluorescent product was measured by fluorescence using a microplate reader (SPECTRAmax GeminiEM, Molecular Devices). References cited Alldredge, A. L., Passow, U. & Logan B. E. 1993. The abundance and significance of a class of large, transparent organic particles in the ocean. Deep-Sea Res. Oceanogr., I. 40: 1131-1140. doi:[10.1016/0967-0637(93)90129-Q](\\"https://dx.doi.org/10.1016/0967-0637%2893%2990129-Q\\") Arar, E. J. & Collins, G. B. 1997. Method 445.0. In Vitro Determination of Chlorophyll a and Pheophytin a in Marine and Freshwater Algae by Fluorescence U.S. Environmental Protection Agency, Cincinnati, Ohio. Berges, J. A., Franklin D. J. & Harrison, P. J. 2001. Evolution of an artificial seawater medium: Improvements in enriched seawater, artificial water over the last two decades. J. Phycol. 37:1138-1145. doi:[10.1046/j.1529-8817.2001.01052.x](\\"https://dx.doi.org/10.1046/j.1529-8817.2001.01052.x\\") Bouchard, J. N., Purdie, D. A. 2011. Effect of elevated temperature, darkness, and hydrogen peroxide treatment on oxidative stress and cell death in the bloom-forming toxic cyanobacterium Microcystis aeruginosa. J. Phycol., 47(6), 1316-1325. doi:[10.1111/j.1529-8817.2011.01074.x](\\"https://dx.doi.org/10.1111/j.1529-8817.2011.01074.x\\") Engel, A. 2009. Determination of Marine Gel Particles. In Wurl, O. [Ed.] Practical Guidelines for the Analysis of Seawater. CRC Press, Taylor & Francis Group, Boca Raton, Florida, pp.125-142. Franklin, D. J., Airs, R. L., Fernandes, M., Bell, T. G., Bongaerts, R. J., Berges, J. A. & Malin, G. 2012. Identification of senescence and death in Emiliania huxleyi and Thalassiosira pseudonana: Cell staining, chlorophyll alterations, and dimethylsulfoniopropionate (DMSP) metabolism. Limnol. Oceanogr. 57: 305\u2013317. doi:10.4319/lo.2012.57.1.0305 Genty, B., Briantais, J. M., Baker N. R. 1989. The relationship between the quantum yield of photosynthetic electron-transport and quenching of chlorophyll fluorescence, Biochimica et Biophysica Acta, 990(1), 87-92. doi:[10.1016/S0304-4165(89)80016-9](\\"https://dx.doi.org/10.1016/S0304-4165\(89\)80016-9\\") Guillard, R. R. L. & Sieracki, M. S. 2005. Counting cells in cultures with the light microscope. In Andersen R. A. [Ed.] Algal Culturing Techniques. Elsevier Academic Press, Burlington, MA, pp. 239-252. Logan, B. E., Grossart, H. P. & Simon, M. 1994. Direct observation of phytoplankton, TEP and aggregates on polycarbonate filters using brightfield microscopy. J. Plankton Res.16: 1811-1815.doi:[10.1093/plankt/16.12.1811](\\"https://dx.doi.org/10.1093/plankt/16.12.1811\\") Marwood, C. A., Smith, R. E. H., Soloman, K. R., Charlton, M. N., Greenberg, B. M. 1999. Intact and photomodified polycyclic aromatic hydrocarbons inhibit photosynthesis in natural assemblages of Lake Erie phytoplankton exposed to solar radiation. Ecotox Environ Safe 44:322-327. doi:[10.1006/eesa.1999.1840](\\"https://dx.doi.org/10.1006/eesa.1999.1840\\") Parsons, T. R., Maita, Y. & Lalli, C. M. 1984. A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, Oxford, UK. Passow, U. & Alldredge, A. L. 1995. A dye-binding assay for the spectrophotometric measurement of transparent exopolymer particles (TEP). Limnol. Oceanogr. 40: 1326-1335. doi:[10.4319/lo.1995.40.7.1326](\\"https://dx.doi.org/10.4319/lo.1995.40.7.1326\\") Veldhuis, M. J. W., Kraay, G. W. & Timmermans, K. R. 2001. Cell death in phytoplankton: correlation between changes in membrane permeability, photosynthetic activity, pigmentation and growth. Eur. J. Phycol. 36: 167\u2013177. doi:[10.1080/09670260110001735318](\\"https://dx.doi.org/10.1080/09670260110001735318\\") Zack, G. W., Rogers, W.E., Latt S. A. 1977. Automatic-measurement of sister chromatid exchange frequency, J. Histochem. Cytochem., 25(7), 741-753. doi:[10.1177/25.7.70454](\\"https://dx.doi.org/10.1177/25.7.70454\\")
attribute	NC_GLOBAL	awards_0_award_nid	String	55158
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-0726369
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0726369
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	TEP production under oxidative stress PI: Daniel C.O. Thornton (Texas A&M) Version: 15 April 2014
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2014-04-15T14:57:24Z
attribute	NC_GLOBAL	date_modified	String	2019-11-21T17:52:15Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.511217.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/511217
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Fluorometer
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	The quantum yield of photosystem II was measured using the saturating pulse method (Genty et al. 1989) using a pulse amplitude modulated fluorometer (PAM-210, Heinz Walz GmbH).
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	511359
attribute	NC_GLOBAL	instruments_0_description	String	A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/113/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Fluorometer
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	484
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Pulse Amplitude Modulated Fluorometer
attribute	NC_GLOBAL	instruments_1_acronym	String	UV Spectrophotometer-Shimadzu
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Turbidity of the cultures was measured by absorbance at 750 nm in a 1 cm path cuvette using a UV-Mini 1240 Spectrophotometer (Shimadzu Corporation).
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	511356
attribute	NC_GLOBAL	instruments_1_description	String	The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB20/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	UV Spectrophotometer-Shimadzu
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	595
attribute	NC_GLOBAL	instruments_1_supplied_name	String	UV-Mini 1240 Spectrophotometer
attribute	NC_GLOBAL	instruments_2_acronym	String	TD-700
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Chlorophyll a concentration 90% acetone extractions from biomass retained on GF/C (Whatman) were measured using a Turner Designs 700 fluorometer, which was calibrated using chlorophyll a standards (Sigma) (Arar and Collins 1997).
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	511357
attribute	NC_GLOBAL	instruments_2_description	String	The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements.
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0510/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Turner Designs 700 Laboratory Fluorometer
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	694
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Turner Designs 700 Fluorometer
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Cell permeability was determined using an epifluorescence microscope (Axioplan 2, Carl Zeiss MicroImaging).
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	511358
attribute	NC_GLOBAL	instruments_3_description	String	Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute	NC_GLOBAL	instruments_3_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB06/
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Microscope-Fluorescence
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	695
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Epifluorescence Microscope
attribute	NC_GLOBAL	instruments_4_acronym	String	Hemocytometer
attribute	NC_GLOBAL	instruments_4_dataset_instrument_description	String	Counts of 400 cells from each culture were made using hemocytometers (Guillard and Sieracki 2005) from samples preserved in Lugol’s iodine (Parsons et al. 1984) using a light microscope.
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	511354
attribute	NC_GLOBAL	instruments_4_description	String	A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
attribute	NC_GLOBAL	instruments_4_instrument_name	String	Hemocytometer
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	704
attribute	NC_GLOBAL	instruments_4_supplied_name	String	Hemocytometer
attribute	NC_GLOBAL	instruments_5_dataset_instrument_description	String	Counts of 400 cells from each culture were made using hemocytometers (Guillard and Sieracki 2005) from samples preserved in Lugol’s iodine (Parsons et al. 1984) using a light microscope (Axioplan 2, Carl Zeiss MicroImaging). A light microscope (Axioplan 2, Carl Zeiss MicroImaging) was also used to enumerate TEP and CSP by image analysis.
attribute	NC_GLOBAL	instruments_5_dataset_instrument_nid	String	511355
attribute	NC_GLOBAL	instruments_5_description	String	Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute	NC_GLOBAL	instruments_5_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB05/
attribute	NC_GLOBAL	instruments_5_instrument_name	String	Microscope-Optical
attribute	NC_GLOBAL	instruments_5_instrument_nid	String	708
attribute	NC_GLOBAL	instruments_5_supplied_name	String	Light Microscope
attribute	NC_GLOBAL	keywords	String	abund, activity, bco, bco-dmo, biological, caspase, caspase_like_activity, cell, cell_conc, cells, chemical, chemistry, chla, chlorophyll, chlorophyll-a, conc, concentration, concentration_of_chlorophyll_in_sea_water, csp, CSP_abund, CSP_conc, CSP_per_chla, data, dataset, day, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Chlorophyll, erddap, H2O2, like, management, O2, ocean, oceanography, oceans, office, oxygen, pcnt, preliminary, psii, science, sea, seawater, species, stained, stained_cells_pcnt, tep, TEP_abund, TEP_conc, TEP_per_chla, turbidity, water
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/511217/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/511217
attribute	NC_GLOBAL	param_mapping	String	{'511217': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/511217/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Texas A&M University
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	TAMU
attribute	NC_GLOBAL	people_0_person_name	String	Daniel C.O. Thornton
attribute	NC_GLOBAL	people_0_person_nid	String	51644
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_1_person_nid	String	51498
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Diatom EPS Production
attribute	NC_GLOBAL	projects_0_acronym	String	Diatom EPS Production
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF Propsoal: It is necessary to determine the fate of organic matter in the ocean to understand marine food webs, biogeochemical cycles, and climate change. Diatoms fix approximately a quarter of the net global primary production each year, and a significant proportion of this production is excreted as extracellular polymeric substances (EPS). EPS have a profound impact on pelagic ecosystems by affecting the formation of aggregates. Diatoms and other particulate organic carbon (POC) sink rapidly as aggregates, affecting the biological carbon pump, which plays a pivotal role in the sequestration of carbon in the ocean. The proposed research will test the central hypothesis: Temperature increase affects diatom release of EPS, which act as a glue, increasing aggregation. Previous work by the investigator showed that increased temperatures affected the aggregation of Skeletonema costatum. Four specific hypotheses will be tested: H1: Diatoms produce more EPS with increasing temperature. H2: Diatoms produce more transparent exopolymer particles (TEP) with increasing temperature. H3: The quantity or composition of cell-surface carbohydrates in diatoms changes with temperature. H4: Aggregation of diatom cultures and natural plankton increases with temperature. Laboratory experiments (years 1 - 2) will be conducted with three model diatom species grown at controlled growth rates and defined limitation (nitrogen or light) in continuous culture. Culture temperature will be stepped up or down in small increments to determine the effect of the temperature change on EPS production, aggregation, and partitioning of carbon in intra- and extracellular pools. Similar experiments in year 3 will be carried out using natural plankton populations from a coastal site where diatoms contribute a significant proportion to the biomass. The proposed research will increase our understanding of the ecology and physiology of one of the dominant groups of primary producers on Earth. EPS are a central aspect of diatom biology, though the physiology, function and broader ecosystem impacts of EPS production remain unknown. This research will determine how temperature, light limitation, and nutrient limitation affect the partitioning of production between dissolved, gel, and particulate phases in the ocean. Measurements of plankton stickiness (alpha) under different conditions will be important to model aggregation processes in the ocean as alpha is an important (and variable) term in coagulation models. Determining how carbon is cycled between the ocean, atmosphere and lithosphere is key to understanding climate change on both geological and human time scales. This is a major societal issue as atmospheric CO2 concentrations are steadily increasing, correlating with a 0.6 C rise in global average temperature during the last century. This research will address potential feedbacks between warming of the surface ocean, diatom ecophysiology and the biological carbon pump. Related Publications: Rzadkowolski, Charles E. and Thornton, Daniel C. O. (2012) Using laser scattering to identify diatoms and conduct aggregation experiments. Eur. J. Phycol., 47(1): 30-41. DOI: 10.1080/09670262.2011.646314 Thornton, Daniel C. O. (2009) Effect of Low pH on Carbohydrate Production by a Marine Planktonic Diatom (Chaetoceros muelleri). Research Letters in Ecology, vol. 2009, Article ID 105901, 4 pages. DOI: 10.1155/2009/105901 Thornton, D.C.O. (2014) Dissolved organic matter (DOM) release by phytoplankton in the contemporary and future ocean. European Journal of Phycology 49: 20-46. DOI: 10.1080/09670262.2013.875596 Thornton, D.C.O., Visser, L.A. (2009) Measurement of acid polysaccharides (APS) associated with microphytobenthos in salt marsh sediments. Aquat Microb Ecol 54:185-198. DOI: 10.3354/ame01265
attribute	NC_GLOBAL	projects_0_end_date	String	2012-08
attribute	NC_GLOBAL	projects_0_geolocation	String	O&M Building, Texas A&M University, College Station, TX 77840
attribute	NC_GLOBAL	projects_0_name	String	Effect of Temperature on Extracellular Polymeric Substance Production (EPS) by Diatoms
attribute	NC_GLOBAL	projects_0_project_nid	String	2255
attribute	NC_GLOBAL	projects_0_start_date	String	2007-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Data from laboratory experiment on exopolymer production by the diatom Thalassiosira wessiflogii (CCMP 1051) and the cyanobacterium Synechococcus elongates_cf (CCMP 1379) under conditions of oxidative stress.
attribute	NC_GLOBAL	title	String	[oxidative stress TEP] - Experimental results: Exopolymer production by phytoplankton under oxidative stress; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project) (Effect of Temperature on Extracellular Polymeric Substance Production (EPS) by Diatoms)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	species		String
attribute	species	bcodmo_name	String	species
attribute	species	description	String	Species name.
attribute	species	long_name	String	Species
attribute	species	units	String	dimensionless
variable	day		byte
attribute	day	_FillValue	byte	127
attribute	day	actual_range	byte	0, 3
attribute	day	bcodmo_name	String	day
attribute	day	description	String	Day of the experiment.
attribute	day	long_name	String	Day
attribute	day	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/DAYXXXXX/
attribute	day	units	String	dimensionless
variable	H2O2		byte
attribute	H2O2	_FillValue	byte	127
attribute	H2O2	actual_range	byte	0, 100
attribute	H2O2	bcodmo_name	String	Hydrogen Peroxide
attribute	H2O2	description	String	Hydrogen peroxide concentration.
attribute	H2O2	long_name	String	H2 O2
attribute	H2O2	units	String	micromolar (uM)
variable	turbidity		float
attribute	turbidity	_FillValue	float	NaN
attribute	turbidity	actual_range	float	0.0302, 0.1368
attribute	turbidity	bcodmo_name	String	turbidity
attribute	turbidity	description	String	Turbidity of the cultures measured by absorbance at 750 nm in a 1 cm path cuvette using a spectrophotometer.
attribute	turbidity	long_name	String	Turbidity
attribute	turbidity	units	String	NTU
variable	cell_conc		int
attribute	cell_conc	_FillValue	int	2147483647
attribute	cell_conc	actual_range	int	68800, 7510000
attribute	cell_conc	bcodmo_name	String	unknown
attribute	cell_conc	description	String	Cell concentration. Counts of 400 cells were made by transmitted light microscopy using a hemacytometer (Fuchs-Rosenthal ruling Hauser Scientific) (Guillard & Sieracki 2005).
attribute	cell_conc	long_name	String	Cell Conc
attribute	cell_conc	units	String	cells per milliliter (cells mL-1)
variable	chla		float
attribute	chla	_FillValue	float	NaN
attribute	chla	actual_range	float	144.0, 491.67
attribute	chla	bcodmo_name	String	chlorophyll a
attribute	chla	colorBarMaximum	double	30.0
attribute	chla	colorBarMinimum	double	0.03
attribute	chla	colorBarScale	String	Log
attribute	chla	description	String	Chlorophyll a measured by fluorescence (Arar & Collins 1997; Method 445.0. EPA).
attribute	chla	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	chla	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/
attribute	chla	units	String	micrograms per liter (ug L-1)
variable	PSII		float
attribute	PSII	_FillValue	float	NaN
attribute	PSII	actual_range	float	0.2, 0.7
attribute	PSII	bcodmo_name	String	unknown
attribute	PSII	description	String	Quantum yield of photosystem II measured after Marwood et al. (1999) using pulse amplitude modulated chlorophyll fluorometer.
attribute	PSII	long_name	String	PSII
attribute	PSII	units	String	quantum yield of photosystem II
variable	caspase_like_activity		float
attribute	caspase_like_activity	_FillValue	float	NaN
attribute	caspase_like_activity	actual_range	float	79.445, 1787.544
attribute	caspase_like_activity	bcodmo_name	String	unknown
attribute	caspase_like_activity	description	String	Caspase-like activity was measured after Bouchard & Purdie (2011).
attribute	caspase_like_activity	long_name	String	Caspase Like Activity
attribute	caspase_like_activity	units	String	relative fluorescence units per milligrams protein per hour (RFU mg protein-1 h-1)
variable	stained_cells_pcnt		float
attribute	stained_cells_pcnt	_FillValue	float	NaN
attribute	stained_cells_pcnt	actual_range	float	1.0, 80.25
attribute	stained_cells_pcnt	bcodmo_name	String	unknown
attribute	stained_cells_pcnt	description	String	% of SYTOX Green stained cells. Uptake and staining with the membrane-impermeable SYTOX Green (Invitrogen) was used to determine what proportion of the diatom population had permeable cell membranes (Veldhuis et al. 2001; Franklin et al. 2012). Four hundred cells were examined using an epifluorescence microscope and the number of cells that stained with SYTOX Green was enumerated.
attribute	stained_cells_pcnt	long_name	String	Stained Cells Pcnt
attribute	stained_cells_pcnt	units	String	percent (%)
variable	TEP_conc		int
attribute	TEP_conc	_FillValue	int	2147483647
attribute	TEP_conc	actual_range	int	124295402, 769625835
attribute	TEP_conc	bcodmo_name	String	unknown
attribute	TEP_conc	description	String	Transparent exopolymer particles (TEP) concentration. TEP retained on 0.4 polycarbonate filters and stained with Alcian blue (Alldredge et al. 1993).
attribute	TEP_conc	long_name	String	TEP Conc
attribute	TEP_conc	units	String	micrometers TEP per milliliter (um2 mL-1)
variable	TEP_abund		int
attribute	TEP_abund	_FillValue	int	2147483647
attribute	TEP_abund	actual_range	int	485361, 1827594
attribute	TEP_abund	bcodmo_name	String	unknown
attribute	TEP_abund	description	String	Transparent exopolymer particles (TEP) abundance. TEP retained on 0.4 polycarbonate filters and stained with Alcian blue (Alldredge et al. 1993).
attribute	TEP_abund	long_name	String	TEP Abund
attribute	TEP_abund	units	String	TEP per milliliter (mL-1)
variable	TEP_per_chla		float
attribute	TEP_per_chla	_FillValue	float	NaN
attribute	TEP_per_chla	actual_range	float	4.85361E-7, 2.39
attribute	TEP_per_chla	bcodmo_name	String	unknown
attribute	TEP_per_chla	colorBarMaximum	double	30.0
attribute	TEP_per_chla	colorBarMinimum	double	0.03
attribute	TEP_per_chla	colorBarScale	String	Log
attribute	TEP_per_chla	description	String	Transparent exopolymer particles (TEP) per chlorophyll a.
attribute	TEP_per_chla	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	TEP_per_chla	units	String	square millimeters of TEP per nanogram of chla (mm2 (ng chl. a)-1)
variable	CSP_conc		double
attribute	CSP_conc	_FillValue	double	NaN
attribute	CSP_conc	actual_range	double	3717141.325, 3.728874422E8
attribute	CSP_conc	bcodmo_name	String	unknown
attribute	CSP_conc	description	String	Coomassie staining particles (CSP) concentration. CSP retained on 0.4 polycarbonate filters and stained with Coomassie briliant blue blue (Long & Azam 1996).
attribute	CSP_conc	long_name	String	CSP Conc
attribute	CSP_conc	units	String	micrometers CSP per milliliter (um2 mL-1)
variable	CSP_abund		int
attribute	CSP_abund	_FillValue	int	2147483647
attribute	CSP_abund	actual_range	int	44941, 826912
attribute	CSP_abund	bcodmo_name	String	unknown
attribute	CSP_abund	description	String	Coomassie staining particles (CSP) abundance. CSP retained on 0.4 polycarbonate filters and stained with Coomassie briliant blue blue (Long & Azam 1996).
attribute	CSP_abund	long_name	String	CSP Abund
attribute	CSP_abund	units	String	CSP per milliliter (mL-1)
variable	CSP_per_chla		float
attribute	CSP_per_chla	_FillValue	float	NaN
attribute	CSP_per_chla	actual_range	float	0.0156, 1.045
attribute	CSP_per_chla	bcodmo_name	String	unknown
attribute	CSP_per_chla	colorBarMaximum	double	30.0
attribute	CSP_per_chla	colorBarMinimum	double	0.03
attribute	CSP_per_chla	colorBarScale	String	Log
attribute	CSP_per_chla	description	String	Coomassie staining particles (CSP) per chlorophyll a.
attribute	CSP_per_chla	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	CSP_per_chla	units	String	square millimeters of CSP per nanogram of chlorophyll a (mm2 (ng chl. a)-1)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.