ERDDAP > info > bcodmo_dataset_511526

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	Experimental results: Exopolymer and carbohydrate production by diatoms with growth; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project)		I   M	background			BCO-DMO	bcodmo_dataset_511526

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv
attribute	NC_GLOBAL	acquisition_description	String	Growth of the diatoms The diatoms Thalassiosira weissflogii (CCMP 1051), Skeletonema marinoi (CCMP 1332), and Cylindrotheca closterium (CCMP 339) were grown in artificial seawater (Berges et al. 2001) in batch culture at 20 \u00b0C with 100 \u00b5M\u00a0 NaNO3, 200 \u00b5M of NaH2PO4\u00b7H2O, and 200 \u00b5M of Na2SiO3\u00b79H2O. Illumination was on a 14 h:10 h light:dark cycle at a photon flux density of 160 \u00b5mol m-2 s-1. There were three replicate cultures. Cultures were sampled during both the growth and death of the cultures over several weeks. Measures of diatom abundance and biomass Counts of 400 cells from each culture were made using a hemacytometer (Fuchs-Rosenthal ruling, Hauser Scientific) (Guillard and Sieracki 2005) from samples preserved in Lugol\u2019s iodine (Parsons et al. 1984) using a light microscope (Axioplan 2, Carl Zeiss MicroImaging). Turbidity of the cultures, used as an indicator of growth, was measured by absorbance at 750 nm in a 1 cm path cuvette using a UV-Mini 1240 spectrophotometer (Shimadzu Corporation). Cell volume was determined using live cells (Menden-Deuer and Lessard 2000). The volume of 25 diatoms from each replicate culture was determined by measuring cell length (pervalver length) and width (valver length) at 400x magnification using a light microscope (Axioplan 2, Carl Zeiss MicroImaging). Cell volume was calculated based on the assumption that both T. wessiflogii and S. marinoi were cylinders. The volume of Cylindrotheca closterium was estimated assuming that its shape was equivalent to two cones. Chlorophyll a concentration 90% acetone extractions from biomass retained on GF/C (Whatman) were measured using a Turner Designs 700 fluorometer, which was calibrated using chlorophyll a standards (Sigma) (Arar and Collins 1997). The extract was diluted with 90% acetone if the chl a concentration were too high. Bacteria abundance Bacteria (400 cells) were counted using an epifluorescence microscope (Axioplan 2, Carl Zeiss MicroImaging) after staining with 4'6-diamidino-2-phenylindole dihydrochloride (DAPI) (Porter and Feig 1980) at a final concentration of 0.25 \u00b5g ml-1. Carbohydrate analysis Two spectrophotometric methods were used to measure carbohydrates, the phenol sulfuric acid (PSA) method (Dubois et al. 1956) and the 2, 4, 6-tripyridyl-s-triazine (TPTZ) method (Myklestad et al. 1997). The color produced by both methods was measured in 1 cm path length cuvette using UV- Mini 1240 spectrophotometer (Shimadzu Corporation). Both methods were calibrated using D-glucose and the results are expressed as D-glucose equivalents. Different fractions of carbohydrate were extracted from the cultures using methods described in Underwood et al. (1995) and Underwood et al. (2004): total, colloidal, exopolymers (EPS), intracellular carbohydrate (hot water (HW) extraction), cell-wall associated carbohydrates (hot bicarbonate (HB) extraction), and residual. These carbohydrate fractions were measured using the PSA method. The TPTZ method was used to measure the intracellular and extracellular monosaccharide pools and the intracellular and extracellular polysaccharide pools after acid hydrolysis of the sample. Cell permeability Uptake and staining with the membrane-impermeable SYTOX Green (Invitrogen) was used to determine what proportion of the diatom population had permeable cell membranes (Veldhuis et al. 2001, Franklin et al. 2012). Four hundred cells were examined using an epifluorescence microscope (Axioplan 2, Carl Zeiss MicroImaging) and the number of cells that stained with SYTOX Green was enumerated. TEP staining and analysis Transparent exopolymer particles (TEP) were sampled according to Alldredge et al. (1993) and TEP abundance was enumerated by image analysis (Logan et al. 1994, Engel 2009). Ten photomicrographs were taken of each slide using a light microscope (Axioplan 2, Carl Zeiss MicroImaging). Images were analyzed using ImageJ software (National Institutes of Health) based on the method of Engel (2009). Thresholding during image processing was done using the triangle method (Zack et al. 1977). CSP staining and analysis Coomassie staining particles (CSP) were sampled according to Long and Azam et al. (1996) and CSP abundance was enumerated by image analysis (Logan et al. 1994, Engel 2009). Ten photomicrographs were taken of each slide using a light microscope (Axioplan 2, Carl Zeiss MicroImaging). Images were analyzed using ImageJ software (National Institutes of Health) based on the method of Engel (2009). Thresholding during image processing was done using the triangle method (Zack et al. 1977). References cited Alldredge, A. L., Passow, U. & Logan B. E. 1993. The abundance and significance of a class of large, transparent organic particles in the ocean. Deep-Sea Res. Oceanogr., I. 40: 1131-1140. doi:[10.1016/0967-0637(93)90129-Q](\\"https://dx.doi.org/10.1016/0967-0637%2893%2990129-Q\\") Arar, E. J. & Collins, G. B. 1997. Method 445.0. In Vitro Determination of Chlorophyll a and Pheophytin a in Marine and Freshwater Algae by Fluorescence U.S. Environmental Protection Agency, Cincinnati, Ohio. Berges, J. A., Franklin D. J. & Harrison, P. J. 2001. Evolution of an artificial seawater medium: Improvements in enriched seawater, artificial water over the last two decades. J. Phycol. 37:1138-1145. doi:[10.1046/j.1529-8817.2001.01052.x](\\"https://dx.doi.org/10.1046/j.1529-8817.2001.01052.x\\") Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350\u2013356. doi:[10.1021/ac60111a017](\\"https://dx.doi.org/10.1021/ac60111a017\\") Engel, A. 2009. Determination of Marine Gel Particles. In Wurl, O. [Ed.] Practical Guidelines for the Analysis of Seawater. CRC Press, Taylor & Francis Group, Boca Raton, Florida, pp.125-142. Franklin, D. J., Airs, R. L., Fernandes, M., Bell, T. G., Bongaerts, R. J., Berges, J. A. & Malin, G. 2012. Identification of senescence and death in Emiliania huxleyi and Thalassiosira pseudonana: Cell staining, chlorophyll alterations, and dimethylsulfoniopropionate (DMSP) metabolism. Limnol. Oceanogr. 57: 305\u2013317. doi:10.4319/lo.2012.57.1.0305 Guillard, R. R. L. & Sieracki, M. S. 2005. Counting cells in cultures with the light microscope. In Andersen R. A. [Ed.] Algal Culturing Techniques. Elsevier Academic Press, Burlington, MA, pp. 239-252. Logan, B. E., Grossart, H. P. & Simon, M. 1994. Direct observation of phytoplankton, TEP and aggregates on polycarbonate filters using brightfield microscopy. J. Plankton Res.16: 1811-1815.doi:[10.1093/plankt/16.12.1811](\\"https://dx.doi.org/10.1093/plankt/16.12.1811\\") Menden-Deuer S. & Lessard, E. J. 2000. Carbon to volume relationships for dinoflagellates, diatoms, and other protists plankton. Limnol. Oceanogr. 45: 569- 579. doi:[10.4319/lo.2000.45.3.0569](\\"https://dx.doi.org/10.4319/lo.2000.45.3.0569\\") Myklestad, S. M., Skanoy, E., Hestmann S. 1997. A sensitive and rapid method for analysis of dissolved mono- and polysaccharides in seawater. Marine Chemistry 56: 279-286. doi:[10.1016/S0304-4203(96)00074-6](\\"https://dx.doi.org/10.1016/S0304-4203\(96\)00074-6\\") Parsons, T. R., Maita, Y. & Lalli, C. M. 1984. A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, Oxford, UK. Passow, U. & Alldredge, A. L. 1995. A dye-binding assay for the spectrophotometric measurement of transparent exopolymer particles (TEP). Limnol. Oceanogr. 40: 1326-1335. doi:[10.4319/lo.1995.40.7.1326](\\"https://dx.doi.org/10.4319/lo.1995.40.7.1326\\") Porter, K. G. & Feig, Y. S. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25:943\u2013948. doi:[10.4319/lo.1980.25.5.0943](\\"https://dx.doi.org/10.4319/lo.1980.25.5.0943\\") Underwood, G. J. C., Paterson D. M., Parkes R. J. 1995. The measurement of microbial carbohydrate exopolymers from intertidal sediments. Limnol. Oceanogr. 40: 1243-1253. doi:[10.4319/lo.1995.40.7.1243](\\"https://dx.doi.org/10.4319/lo.1995.40.7.1243\\") Underwood, G. J. C., Boulcott, M., Raines, C. A., Waldron K. 2004. Environmental effects on exopolymer production by marine benthic diatoms: Dynamics, changes in composition, and pathways of production. J. Phycol. 40: 293-304. doi:[10.1111/j.1529-8817.2004.03076.x](\\"https://dx.doi.org/10.1111/j.1529-8817.2004.03076.x\\") Veldhuis, M. J. W., Kraay, G. W. & Timmermans, K. R. 2001. Cell death in phytoplankton: correlation between changes in membrane permeability, photosynthetic activity, pigmentation and growth. Eur. J. Phycol. 36: 167\u2013177. doi:[10.1080/09670260110001735318](\\"https://dx.doi.org/10.1080/09670260110001735318\\") Zack, G. W., Rogers, W.E., Latt S. A. 1977. Automatic-measurement of sister chromatid exchange frequency, J. Histochem. Cytochem., 25(7), 741-753. doi:[10.1177/25.7.70454](\\"https://dx.doi.org/10.1177/25.7.70454\\")
attribute	NC_GLOBAL	awards_0_award_nid	String	55158
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-0726369
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=0726369
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Growth phase exopolymer and carbohydrate production by diatoms PI: Daniel C.O. Thornton (Texas A&M) Version: 16 April 2014
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2014-04-16T15:19:09Z
attribute	NC_GLOBAL	date_modified	String	2019-11-21T18:09:10Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.511526.1
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/511526
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	UV Spectrophotometer-Shimadzu
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Turbidity of the cultures was measured by absorbance at 750 nm in a 1 cm path cuvette using a UV-Mini 1240 spectrophotometer (Shimadzu Corporation). Two spectrophotometric methods were used to measure carbohydrates, the phenol sulfuric acid (PSA) method (Dubois et al. 1956) and the 2, 4, 6-tripyridyl-s-triazine (TPTZ) method (Myklestad et al. 1997). The color produced by both methods was measured in 1 cm path length cuvette using UV-Mini 1240 spectrophotometer (Shimadzu Corporation).
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	511581
attribute	NC_GLOBAL	instruments_0_description	String	The Shimadzu UV Spectrophotometer is manufactured by Shimadzu Scientific Instruments (ssi.shimadzu.com). Shimadzu manufacturers several models of spectrophotometer; refer to dataset for make/model information.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB20/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	UV Spectrophotometer-Shimadzu
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	595
attribute	NC_GLOBAL	instruments_0_supplied_name	String	UV-Mini 1240 Spectrophotometer
attribute	NC_GLOBAL	instruments_1_acronym	String	TD-700
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Chlorophyll a concentration 90% acetone extractions from biomass retained on GF/C (Whatman) were measured using a Turner Designs 700 fluorometer, which was calibrated using chlorophyll a standards (Sigma) (Arar and Collins 1997).
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	511582
attribute	NC_GLOBAL	instruments_1_description	String	The TD-700 Laboratory Fluorometer is a benchtop fluorometer designed to detect fluorescence over the UV to red range. The instrument can measure concentrations of a variety of compounds, including chlorophyll-a and fluorescent dyes, and is thus suitable for a range of applications, including chlorophyll, water quality monitoring and fluorescent tracer studies. Data can be output as concentrations or raw fluorescence measurements.
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0510/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Turner Designs 700 Laboratory Fluorometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	694
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Turner Designs 700 Fluorometer
attribute	NC_GLOBAL	instruments_2_dataset_instrument_description	String	Bacteria were counted and cell permeability was determined using an epifluorescence microscope (Axioplan 2, Carl Zeiss MicroImaging).
attribute	NC_GLOBAL	instruments_2_dataset_instrument_nid	String	511583
attribute	NC_GLOBAL	instruments_2_description	String	Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.
attribute	NC_GLOBAL	instruments_2_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB06/
attribute	NC_GLOBAL	instruments_2_instrument_name	String	Microscope-Fluorescence
attribute	NC_GLOBAL	instruments_2_instrument_nid	String	695
attribute	NC_GLOBAL	instruments_2_supplied_name	String	Epifluorescence Microscope
attribute	NC_GLOBAL	instruments_3_acronym	String	Hemocytometer
attribute	NC_GLOBAL	instruments_3_dataset_instrument_description	String	Counts of 400 cells from each culture were made using a hemocytometer (Fuchs-Rosenthal ruling, Hauser Scientific) (Guillard and Sieracki 2005) from samples preserved in Lugol’s iodine (Parsons et al. 1984) using a light microscope.
attribute	NC_GLOBAL	instruments_3_dataset_instrument_nid	String	511579
attribute	NC_GLOBAL	instruments_3_description	String	A hemocytometer is a small glass chamber, resembling a thick microscope slide, used for determining the number of cells per unit volume of a suspension. Originally used for performing blood cell counts, a hemocytometer can be used to count a variety of cell types in the laboratory. Also spelled as "haemocytometer". Description from: http://hlsweb.dmu.ac.uk/ahs/elearning/RITA/Haem1/Haem1.html.
attribute	NC_GLOBAL	instruments_3_instrument_name	String	Hemocytometer
attribute	NC_GLOBAL	instruments_3_instrument_nid	String	704
attribute	NC_GLOBAL	instruments_3_supplied_name	String	Hemocytometer
attribute	NC_GLOBAL	instruments_4_dataset_instrument_description	String	Counts of 400 cells from each culture were made using a hemacytometer (Fuchs-Rosenthal ruling, Hauser Scientific) (Guillard and Sieracki 2005) from samples preserved in Lugol’s iodine (Parsons et al. 1984) using a light microscope (Axioplan 2, Carl Zeiss MicroImaging). A light microscope was also used to determine cell volume and to enumerate TEP and CSP by image analysis.
attribute	NC_GLOBAL	instruments_4_dataset_instrument_nid	String	511580
attribute	NC_GLOBAL	instruments_4_description	String	Instruments that generate enlarged images of samples using the phenomena of reflection and absorption of visible light. Includes conventional and inverted instruments. Also called a "light microscope".
attribute	NC_GLOBAL	instruments_4_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB05/
attribute	NC_GLOBAL	instruments_4_instrument_name	String	Microscope-Optical
attribute	NC_GLOBAL	instruments_4_instrument_nid	String	708
attribute	NC_GLOBAL	instruments_4_supplied_name	String	Light Microscope
attribute	NC_GLOBAL	keywords	String	abundance, area, bact, bact_per_diatom, bacteria, bco, bco-dmo, biological, carb, cell, cell_abundance, cell_vol_mean, cells, chemical, chemistry, chla, chla_per_cell, chla_per_cell_vol, chlorophyll, chlorophyll-a, collodial, collodial_per_cell, colloidal, colloidal_carb, colloidal_per_cell_vol, conc, concentration, concentration_of_chlorophyll_in_sea_water, csp, CSP_conc_mean, CSP_mean_size, culture, data, dataset, day, diatom, dmo, earth, Earth Science > Oceans > Ocean Chemistry > Chlorophyll, eps, EPS_carb, EPS_carb_per_cell, EPS_carb_per_cell_vol, erddap, extracell, growth, growth_phase, HB_carb, HB_carb_per_cell, HB_carb_per_cell_vol, HW_carb, HW_carb_per_cell, HW_carb_per_cell_vol, intracell, management, mean, mono, ocean, oceanography, oceans, office, pcnt, per, phase, polysacc, preliminary, residual, residual_carb, residual_carb_per_cell, residual_carb_per_cell_vol, science, sea, seawater, size, species, stained, stained_cells_pcnt, tep, TEP_conc_mean, TEP_mean_size, tot, tot_carb, tot_carb_per_cell, tot_carb_per_cell_vol, tot_CSP_area, tot_TEP_area, tptz, TPTZ_extracell_mono, TPTZ_extracell_polysacc, TPTZ_intracell_mono, TPTZ_intracell_polysacc, vol, water
attribute	NC_GLOBAL	keywords_vocabulary	String	GCMD Science Keywords
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/511526/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/511526
attribute	NC_GLOBAL	param_mapping	String	{'511526': {}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/511526/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	Texas A&M University
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	TAMU
attribute	NC_GLOBAL	people_0_person_name	String	Daniel C.O. Thornton
attribute	NC_GLOBAL	people_0_person_nid	String	51644
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_1_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_1_person_nid	String	51498
attribute	NC_GLOBAL	people_1_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	project	String	Diatom EPS Production
attribute	NC_GLOBAL	projects_0_acronym	String	Diatom EPS Production
attribute	NC_GLOBAL	projects_0_description	String	Description from NSF Propsoal: It is necessary to determine the fate of organic matter in the ocean to understand marine food webs, biogeochemical cycles, and climate change. Diatoms fix approximately a quarter of the net global primary production each year, and a significant proportion of this production is excreted as extracellular polymeric substances (EPS). EPS have a profound impact on pelagic ecosystems by affecting the formation of aggregates. Diatoms and other particulate organic carbon (POC) sink rapidly as aggregates, affecting the biological carbon pump, which plays a pivotal role in the sequestration of carbon in the ocean. The proposed research will test the central hypothesis: Temperature increase affects diatom release of EPS, which act as a glue, increasing aggregation. Previous work by the investigator showed that increased temperatures affected the aggregation of Skeletonema costatum. Four specific hypotheses will be tested: H1: Diatoms produce more EPS with increasing temperature. H2: Diatoms produce more transparent exopolymer particles (TEP) with increasing temperature. H3: The quantity or composition of cell-surface carbohydrates in diatoms changes with temperature. H4: Aggregation of diatom cultures and natural plankton increases with temperature. Laboratory experiments (years 1 - 2) will be conducted with three model diatom species grown at controlled growth rates and defined limitation (nitrogen or light) in continuous culture. Culture temperature will be stepped up or down in small increments to determine the effect of the temperature change on EPS production, aggregation, and partitioning of carbon in intra- and extracellular pools. Similar experiments in year 3 will be carried out using natural plankton populations from a coastal site where diatoms contribute a significant proportion to the biomass. The proposed research will increase our understanding of the ecology and physiology of one of the dominant groups of primary producers on Earth. EPS are a central aspect of diatom biology, though the physiology, function and broader ecosystem impacts of EPS production remain unknown. This research will determine how temperature, light limitation, and nutrient limitation affect the partitioning of production between dissolved, gel, and particulate phases in the ocean. Measurements of plankton stickiness (alpha) under different conditions will be important to model aggregation processes in the ocean as alpha is an important (and variable) term in coagulation models. Determining how carbon is cycled between the ocean, atmosphere and lithosphere is key to understanding climate change on both geological and human time scales. This is a major societal issue as atmospheric CO2 concentrations are steadily increasing, correlating with a 0.6 C rise in global average temperature during the last century. This research will address potential feedbacks between warming of the surface ocean, diatom ecophysiology and the biological carbon pump. Related Publications: Rzadkowolski, Charles E. and Thornton, Daniel C. O. (2012) Using laser scattering to identify diatoms and conduct aggregation experiments. Eur. J. Phycol., 47(1): 30-41. DOI: 10.1080/09670262.2011.646314 Thornton, Daniel C. O. (2009) Effect of Low pH on Carbohydrate Production by a Marine Planktonic Diatom (Chaetoceros muelleri). Research Letters in Ecology, vol. 2009, Article ID 105901, 4 pages. DOI: 10.1155/2009/105901 Thornton, D.C.O. (2014) Dissolved organic matter (DOM) release by phytoplankton in the contemporary and future ocean. European Journal of Phycology 49: 20-46. DOI: 10.1080/09670262.2013.875596 Thornton, D.C.O., Visser, L.A. (2009) Measurement of acid polysaccharides (APS) associated with microphytobenthos in salt marsh sediments. Aquat Microb Ecol 54:185-198. DOI: 10.3354/ame01265
attribute	NC_GLOBAL	projects_0_end_date	String	2012-08
attribute	NC_GLOBAL	projects_0_geolocation	String	O&M Building, Texas A&M University, College Station, TX 77840
attribute	NC_GLOBAL	projects_0_name	String	Effect of Temperature on Extracellular Polymeric Substance Production (EPS) by Diatoms
attribute	NC_GLOBAL	projects_0_project_nid	String	2255
attribute	NC_GLOBAL	projects_0_start_date	String	2007-09
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Data from laboratory experiment on exopolymer and carbohydrate production by the diatoms Thalassiosira weissflogii (CCMP 1051), Skeletonema marinoi (CCMP 1332), and Cylindrotheca closterium (CCMP 339) during the growth to death phases of the cultures. Related references: Chen, J. 2014. Factors affecting carbohydrate production and the formation of transparent exopolymer particles (TEP) by diatoms. Ph.D. dissertation, Texas A&M University, College Station, TX.
attribute	NC_GLOBAL	title	String	Experimental results: Exopolymer and carbohydrate production by diatoms with growth; conducted at the Thornton lab, TAMU from 2007-2012 (Diatom EPS Production project)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	species		String
attribute	species	bcodmo_name	String	species
attribute	species	description	String	Species name.
attribute	species	long_name	String	Species
attribute	species	units	String	dimensionless
variable	growth_phase		String
attribute	growth_phase	bcodmo_name	String	unknown
attribute	growth_phase	description	String	Growth phase of the diatom (exponential, stationary, declining, death).
attribute	growth_phase	long_name	String	Growth Phase
attribute	growth_phase	units	String	dimensionless
variable	day		byte
attribute	day	_FillValue	byte	127
attribute	day	actual_range	byte	6, 43
attribute	day	bcodmo_name	String	unknown
attribute	day	description	String	Day of the experiment.
attribute	day	long_name	String	Day
attribute	day	units	String	dimensionless
variable	culture		byte
attribute	culture	_FillValue	byte	127
attribute	culture	actual_range	byte	1, 3
attribute	culture	bcodmo_name	String	replicate
attribute	culture	description	String	Identifier of the culture replicate.
attribute	culture	long_name	String	Culture
attribute	culture	units	String	dimensionless
variable	cell_abundance		float
attribute	cell_abundance	_FillValue	float	NaN
attribute	cell_abundance	actual_range	float	22700.0, 560970.0
attribute	cell_abundance	bcodmo_name	String	diatom
attribute	cell_abundance	description	String	Cell count. Counts of 400 cells were made by transmitted light microscopy using a hemacytometer (Fuchs-Rosenthal ruling Hauser Scientific) (Guillard & Sieracki 2005).
attribute	cell_abundance	long_name	String	Cell Abundance
attribute	cell_abundance	units	String	cells per milliliter (mL-1)
variable	cell_vol_mean		float
attribute	cell_vol_mean	_FillValue	float	NaN
attribute	cell_vol_mean	actual_range	float	21.73, 567.15
attribute	cell_vol_mean	bcodmo_name	String	unknown
attribute	cell_vol_mean	description	String	Mean cell volume calculated assuming that both T. wessiflogii and S. marinoi were cylinders. The volume of Cylindrotheca closterium was estimated assuming that its shape was equivalent to two cones.
attribute	cell_vol_mean	long_name	String	Cell Vol Mean
attribute	cell_vol_mean	units	String	cubic micrometers (um^3)
variable	chla		float
attribute	chla	_FillValue	float	NaN
attribute	chla	actual_range	float	0.02, 366.79
attribute	chla	bcodmo_name	String	chlorophyll a
attribute	chla	colorBarMaximum	double	30.0
attribute	chla	colorBarMinimum	double	0.03
attribute	chla	colorBarScale	String	Log
attribute	chla	description	String	Concentration of chlorophyll a measured by fluorescence (Arar & Collins 1997; Method 445.0. EPA).
attribute	chla	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	chla	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/CPHLHPP1/
attribute	chla	units	String	micrograms per liter (ug L-1)
variable	chla_per_cell		float
attribute	chla_per_cell	_FillValue	float	NaN
attribute	chla_per_cell	actual_range	float	0.0, 5.14
attribute	chla_per_cell	bcodmo_name	String	unknown
attribute	chla_per_cell	colorBarMaximum	double	30.0
attribute	chla_per_cell	colorBarMinimum	double	0.03
attribute	chla_per_cell	colorBarScale	String	Log
attribute	chla_per_cell	description	String	Concentration of chlorophyll a per cell.
attribute	chla_per_cell	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	chla_per_cell	units	String	picograms per cell (pg cell-1)
variable	chla_per_cell_vol		float
attribute	chla_per_cell_vol	_FillValue	float	NaN
attribute	chla_per_cell_vol	actual_range	float	0.0, 94.0
attribute	chla_per_cell_vol	bcodmo_name	String	unknown
attribute	chla_per_cell_vol	colorBarMaximum	double	30.0
attribute	chla_per_cell_vol	colorBarMinimum	double	0.03
attribute	chla_per_cell_vol	colorBarScale	String	Log
attribute	chla_per_cell_vol	description	String	Concentration of chlorophyll a per cell volume.
attribute	chla_per_cell_vol	long_name	String	Concentration Of Chlorophyll In Sea Water
attribute	chla_per_cell_vol	units	String	femtograms per cubic micrometer (fg um-3)
variable	tot_carb		float
attribute	tot_carb	_FillValue	float	NaN
attribute	tot_carb	actual_range	float	1.95, 52.48
attribute	tot_carb	bcodmo_name	String	unknown
attribute	tot_carb	description	String	Total carbohydrate concentration measured using the PSA method (Dubois et al. 1956).
attribute	tot_carb	long_name	String	Tot Carb
attribute	tot_carb	units	String	micrograms per milliliter (ug mL-1)
variable	tot_carb_per_cell		float
attribute	tot_carb_per_cell	_FillValue	float	NaN
attribute	tot_carb_per_cell	actual_range	float	8.52, 496.09
attribute	tot_carb_per_cell	bcodmo_name	String	unknown
attribute	tot_carb_per_cell	description	String	Total carbohydrate concentration per cell.
attribute	tot_carb_per_cell	long_name	String	Tot Carb Per Cell
attribute	tot_carb_per_cell	units	String	picograms per cell (pg cell-1)
variable	tot_carb_per_cell_vol		float
attribute	tot_carb_per_cell_vol	_FillValue	float	NaN
attribute	tot_carb_per_cell_vol	actual_range	float	62.44, 16122.66
attribute	tot_carb_per_cell_vol	bcodmo_name	String	unknown
attribute	tot_carb_per_cell_vol	description	String	Total carbohydrate concentration per cell volume.
attribute	tot_carb_per_cell_vol	long_name	String	Tot Carb Per Cell Vol
attribute	tot_carb_per_cell_vol	units	String	femtograms per cubic micrometer (fg um-3)
variable	colloidal_carb		double
attribute	colloidal_carb	_FillValue	double	NaN
attribute	colloidal_carb	actual_range	double	0.01, 26.31
attribute	colloidal_carb	bcodmo_name	String	unknown
attribute	colloidal_carb	description	String	Colloidal carbohydrate concentration. Different fractions of carbohydrate were extracted from the cultures using methods described in Underwood et al. (1995) and Underwood et al. (2004). The colloidal carbohydrate fractions were measured using the PSA method (Dubois et al. 1956).
attribute	colloidal_carb	long_name	String	Colloidal Carb
attribute	colloidal_carb	units	String	micrograms per milliliter (ug mL-1)
variable	collodial_per_cell		float
attribute	collodial_per_cell	_FillValue	float	NaN
attribute	collodial_per_cell	actual_range	float	0.05, 684.56
attribute	collodial_per_cell	bcodmo_name	String	unknown
attribute	collodial_per_cell	description	String	Colloidal carbohydrate concentration per cell.
attribute	collodial_per_cell	long_name	String	Collodial Per Cell
attribute	collodial_per_cell	units	String	picograms per cell (pg cell-1)
variable	colloidal_per_cell_vol		float
attribute	colloidal_per_cell_vol	_FillValue	float	NaN
attribute	colloidal_per_cell_vol	actual_range	float	0.2, 18219.25
attribute	colloidal_per_cell_vol	bcodmo_name	String	unknown
attribute	colloidal_per_cell_vol	description	String	Colloidal carbohydrate concentration per cell volume.
attribute	colloidal_per_cell_vol	long_name	String	Colloidal Per Cell Vol
attribute	colloidal_per_cell_vol	units	String	femtograms per cubic micrometer (fg um-3)
variable	EPS_carb		float
attribute	EPS_carb	_FillValue	float	NaN
attribute	EPS_carb	actual_range	float	0.17, 4.14
attribute	EPS_carb	bcodmo_name	String	unknown
attribute	EPS_carb	description	String	Exopolymer (EPS) carbohydrate concentration. Different fractions of carbohydrate were extracted from the cultures using methods described in Underwood et al. (1995) and Underwood et al. (2004). The EPS carbohydrate fractions were measured using the PSA method (Dubois et al. 1956).
attribute	EPS_carb	long_name	String	EPS Carb
attribute	EPS_carb	units	String	micrograms per milliliter (ug mL-1)
variable	EPS_carb_per_cell		float
attribute	EPS_carb_per_cell	_FillValue	float	NaN
attribute	EPS_carb_per_cell	actual_range	float	0.61, 49.57
attribute	EPS_carb_per_cell	bcodmo_name	String	unknown
attribute	EPS_carb_per_cell	description	String	Exopolymer (EPS) carbohydrate concentration per cell.
attribute	EPS_carb_per_cell	long_name	String	EPS Carb Per Cell
attribute	EPS_carb_per_cell	units	String	picograms per cell (pg cell-1)
variable	EPS_carb_per_cell_vol		float
attribute	EPS_carb_per_cell_vol	_FillValue	float	NaN
attribute	EPS_carb_per_cell_vol	actual_range	float	2.07, 1043.18
attribute	EPS_carb_per_cell_vol	bcodmo_name	String	unknown
attribute	EPS_carb_per_cell_vol	description	String	Exopolymer (EPS) carbohydrate concentration per cell volume.
attribute	EPS_carb_per_cell_vol	long_name	String	EPS Carb Per Cell Vol
attribute	EPS_carb_per_cell_vol	units	String	femtograms per cubic micrometer (fg um-3)
variable	HW_carb		float
attribute	HW_carb	_FillValue	float	NaN
attribute	HW_carb	actual_range	float	0.12, 29.05
attribute	HW_carb	bcodmo_name	String	unknown
attribute	HW_carb	description	String	Intracellular carbohydrate (hot water (HW) extraction) concentration. Different fractions of carbohydrate were extracted from the cultures using methods described in Underwood et al. (1995) and Underwood et al. (2004). The HW carbohydrate fractions were measured using the PSA method (Dubois et al. 1956).
attribute	HW_carb	long_name	String	HW Carb
attribute	HW_carb	units	String	micrograms per milliliter (ug mL-1)
variable	HW_carb_per_cell		float
attribute	HW_carb_per_cell	_FillValue	float	NaN
attribute	HW_carb_per_cell	actual_range	float	1.88, 170.88
attribute	HW_carb_per_cell	bcodmo_name	String	unknown
attribute	HW_carb_per_cell	description	String	Intracellular carbohydrate (hot water (HW) extraction) concentration per cell.
attribute	HW_carb_per_cell	long_name	String	HW Carb Per Cell
attribute	HW_carb_per_cell	units	String	picograms per cell (pg cell-1)
variable	HW_carb_per_cell_vol		float
attribute	HW_carb_per_cell_vol	_FillValue	float	NaN
attribute	HW_carb_per_cell_vol	actual_range	float	7.93, 1752.36
attribute	HW_carb_per_cell_vol	bcodmo_name	String	unknown
attribute	HW_carb_per_cell_vol	description	String	Intracellular carbohydrate (hot water (HW) extraction) concentration per cell volume.
attribute	HW_carb_per_cell_vol	long_name	String	HW Carb Per Cell Vol
attribute	HW_carb_per_cell_vol	units	String	femtograms per cubic micrometer (fg um-3)
variable	HB_carb		float
attribute	HB_carb	_FillValue	float	NaN
attribute	HB_carb	actual_range	float	0.24, 9.08
attribute	HB_carb	bcodmo_name	String	unknown
attribute	HB_carb	description	String	Cell-wall associated carbohydrate (hot bicarbonate (HB) extraction) concentration. Different fractions of carbohydrate were extracted from the cultures using methods described in Underwood et al. (1995) and Underwood et al. (2004). The HB carbohydrate fractions were measured using the PSA method (Dubois et al. 1956).
attribute	HB_carb	long_name	String	HB Carb
attribute	HB_carb	units	String	micrograms per milliliter (ug mL-1)
variable	HB_carb_per_cell		float
attribute	HB_carb_per_cell	_FillValue	float	NaN
attribute	HB_carb_per_cell	actual_range	float	0.93, 135.08
attribute	HB_carb_per_cell	bcodmo_name	String	unknown
attribute	HB_carb_per_cell	description	String	Cell-wall associated carbohydrate (hot bicarbonate (HB) extraction) concentration per cell.
attribute	HB_carb_per_cell	long_name	String	HB Carb Per Cell
attribute	HB_carb_per_cell	units	String	picograms per cell (pg cell-1)
variable	HB_carb_per_cell_vol		float
attribute	HB_carb_per_cell_vol	_FillValue	float	NaN
attribute	HB_carb_per_cell_vol	actual_range	float	10.0, 3951.35
attribute	HB_carb_per_cell_vol	bcodmo_name	String	unknown
attribute	HB_carb_per_cell_vol	description	String	Cell-wall associated carbohydrate (hot bicarbonate (HB) extraction) concentration per cell volume.
attribute	HB_carb_per_cell_vol	long_name	String	HB Carb Per Cell Vol
attribute	HB_carb_per_cell_vol	units	String	femtograms per cubic micrometer (fg um-3)
variable	residual_carb		float
attribute	residual_carb	_FillValue	float	NaN
attribute	residual_carb	actual_range	float	0.11, 5.07
attribute	residual_carb	bcodmo_name	String	unknown
attribute	residual_carb	description	String	Residual carbohydrate concentration. Different fractions of carbohydrate were extracted from the cultures using methods described in Underwood et al. (1995) and Underwood et al. (2004). The residual carbohydrate fractions were measured using the PSA method (Dubois et al. 1956).
attribute	residual_carb	long_name	String	Residual Carb
attribute	residual_carb	units	String	micrograms per milliliter (ug mL-1)
variable	residual_carb_per_cell		float
attribute	residual_carb_per_cell	_FillValue	float	NaN
attribute	residual_carb_per_cell	actual_range	float	0.33, 50.91
attribute	residual_carb_per_cell	bcodmo_name	String	unknown
attribute	residual_carb_per_cell	description	String	Residual carbohydrate concentration per cell.
attribute	residual_carb_per_cell	long_name	String	Residual Carb Per Cell
attribute	residual_carb_per_cell	units	String	picograms per cell (pg cell-1)
variable	residual_carb_per_cell_vol		float
attribute	residual_carb_per_cell_vol	_FillValue	float	NaN
attribute	residual_carb_per_cell_vol	actual_range	float	1.36, 738.83
attribute	residual_carb_per_cell_vol	bcodmo_name	String	unknown
attribute	residual_carb_per_cell_vol	description	String	Residual carbohydrate concentration per cell volume.
attribute	residual_carb_per_cell_vol	long_name	String	Residual Carb Per Cell Vol
attribute	residual_carb_per_cell_vol	units	String	femtograms per cubic micrometer (fg um-3)
variable	TPTZ_intracell_mono		float
attribute	TPTZ_intracell_mono	_FillValue	float	NaN
attribute	TPTZ_intracell_mono	actual_range	float	0.02, 1.82
attribute	TPTZ_intracell_mono	bcodmo_name	String	unknown
attribute	TPTZ_intracell_mono	description	String	Intracellular monosaccharide concentration determined using the TPTZ method (Myklestad et al. 1997).
attribute	TPTZ_intracell_mono	long_name	String	TPTZ Intracell Mono
attribute	TPTZ_intracell_mono	units	String	micrograms per milliliter (ug mL-1)
variable	TPTZ_extracell_mono		float
attribute	TPTZ_extracell_mono	_FillValue	float	NaN
attribute	TPTZ_extracell_mono	actual_range	float	0.09, 0.97
attribute	TPTZ_extracell_mono	bcodmo_name	String	unknown
attribute	TPTZ_extracell_mono	description	String	Extracellular monosaccharide concentration determined using the TPTZ method (Myklestad et al. 1997).
attribute	TPTZ_extracell_mono	long_name	String	TPTZ Extracell Mono
attribute	TPTZ_extracell_mono	units	String	micrograms per milliliter (ug mL-1)
variable	TPTZ_intracell_polysacc		float
attribute	TPTZ_intracell_polysacc	_FillValue	float	NaN
attribute	TPTZ_intracell_polysacc	actual_range	float	0.2, 7.75
attribute	TPTZ_intracell_polysacc	bcodmo_name	String	unknown
attribute	TPTZ_intracell_polysacc	description	String	Intracellular polysaccharide concentration determined using the TPTZ method (Myklestad et al. 1997).
attribute	TPTZ_intracell_polysacc	long_name	String	TPTZ Intracell Polysacc
attribute	TPTZ_intracell_polysacc	units	String	micrograms per milliliter (ug mL-1)
variable	TPTZ_extracell_polysacc		float
attribute	TPTZ_extracell_polysacc	_FillValue	float	NaN
attribute	TPTZ_extracell_polysacc	actual_range	float	0.04, 2.31
attribute	TPTZ_extracell_polysacc	bcodmo_name	String	unknown
attribute	TPTZ_extracell_polysacc	description	String	Extracellular polysaccharide concentration determined using the TPTZ method (Myklestad et al. 1997).
attribute	TPTZ_extracell_polysacc	long_name	String	TPTZ Extracell Polysacc
attribute	TPTZ_extracell_polysacc	units	String	micrograms per milliliter (ug mL-1)
variable	TEP_conc_mean		double
attribute	TEP_conc_mean	_FillValue	double	NaN
attribute	TEP_conc_mean	actual_range	double	754623.41, 5.429096222E7
attribute	TEP_conc_mean	bcodmo_name	String	unknown
attribute	TEP_conc_mean	description	String	Mean transparent exopolymer particle (TEP) concentration. TEP retained on 0.4 polycarbonate filters and stained with Alcian blue (Alldredge et al. 1993).
attribute	TEP_conc_mean	long_name	String	TEP Conc Mean
attribute	TEP_conc_mean	units	String	TEP per milliliter (TEP mL-1)
variable	TEP_mean_size		float
attribute	TEP_mean_size	_FillValue	float	NaN
attribute	TEP_mean_size	actual_range	float	62.13, 1059.08
attribute	TEP_mean_size	bcodmo_name	String	unknown
attribute	TEP_mean_size	description	String	Mean size of Transparent exopolymer particles (TEP).
attribute	TEP_mean_size	long_name	String	TEP Mean Size
attribute	TEP_mean_size	units	String	square micrometers (um^2)
variable	tot_TEP_area		float
attribute	tot_TEP_area	_FillValue	float	NaN
attribute	tot_TEP_area	actual_range	float	89.68, 8358.71
attribute	tot_TEP_area	bcodmo_name	String	unknown
attribute	tot_TEP_area	description	String	Total TEP area.
attribute	tot_TEP_area	long_name	String	Tot TEP Area
attribute	tot_TEP_area	units	String	square millimeters per milliliter (mm^2 mL-1)
variable	CSP_conc_mean		double
attribute	CSP_conc_mean	_FillValue	double	NaN
attribute	CSP_conc_mean	actual_range	double	227584.84, 1.766777039E7
attribute	CSP_conc_mean	bcodmo_name	String	unknown
attribute	CSP_conc_mean	description	String	Mean coomassie staining particle (CSP) concentration. CSP retained on 0.4 polycarbonate filters and stained with Coomassie briliant blue blue (Long & Azam 1996).
attribute	CSP_conc_mean	long_name	String	CSP Conc Mean
attribute	CSP_conc_mean	units	String	CSP per milliliter (mL-1)
variable	CSP_mean_size		float
attribute	CSP_mean_size	_FillValue	float	NaN
attribute	CSP_mean_size	actual_range	float	30.41, 411.4
attribute	CSP_mean_size	bcodmo_name	String	unknown
attribute	CSP_mean_size	description	String	Mean size of coomassie staining particle (CSP).
attribute	CSP_mean_size	long_name	String	CSP Mean Size
attribute	CSP_mean_size	units	String	square micrometers (um^2)
variable	tot_CSP_area		float
attribute	tot_CSP_area	_FillValue	float	NaN
attribute	tot_CSP_area	actual_range	float	12.69, 5529.74
attribute	tot_CSP_area	bcodmo_name	String	unknown
attribute	tot_CSP_area	description	String	Total CSP area.
attribute	tot_CSP_area	long_name	String	Tot CSP Area
attribute	tot_CSP_area	units	String	square millimeters per milliliter (mm^2 mL-1)
variable	stained_cells_pcnt		float
attribute	stained_cells_pcnt	_FillValue	float	NaN
attribute	stained_cells_pcnt	actual_range	float	2.5, 95.5
attribute	stained_cells_pcnt	bcodmo_name	String	unknown
attribute	stained_cells_pcnt	description	String	% of SYTOX Green stained cells. Cell permeability was determined by SYTOX Green staining (Veldhuis et al. 1997). Four hundred cells were examined using an epifluorescence microscope and the number of cells that stained with SYTOX Green was enumerated.
attribute	stained_cells_pcnt	long_name	String	Stained Cells Pcnt
attribute	stained_cells_pcnt	units	String	percent (%)
variable	bacteria		double
attribute	bacteria	_FillValue	double	NaN
attribute	bacteria	actual_range	double	5356.54, 648811.25
attribute	bacteria	bcodmo_name	String	bact_abundance
attribute	bacteria	description	String	Bacteria abundance determined by DAPI staining and counts using an epifluorescence microscope (Porter & Feig 1980).
attribute	bacteria	long_name	String	Bacteria
attribute	bacteria	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P02/current/BNTX
attribute	bacteria	units	String	cells per milliliter (mL-1)
variable	bact_per_diatom		float
attribute	bact_per_diatom	_FillValue	float	NaN
attribute	bact_per_diatom	actual_range	float	0.03, 25.04
attribute	bact_per_diatom	bcodmo_name	String	unknown
attribute	bact_per_diatom	description	String	Bacteria abundance per diatom.
attribute	bact_per_diatom	long_name	String	Bact Per Diatom
attribute	bact_per_diatom	units	String	dimensionless

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.