ERDDAP > info > bcodmo_dataset_516204

Grid DAP Data	Sub- set	Table DAP Data	Make A Graph	W M S	Source Data Files	Acces- sible	Title	Sum- mary	FGDC, ISO, Metadata	Back- ground Info	RSS	E mail	Institution	Dataset ID
		data	graph		files	public	[bacteria_FCM_lab] - Heterotrophic (non-pigmented) bacteria abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (Costa Rica Dome FLUx and Zinc Experiments)		F   I   M	background			BCO-DMO	bcodmo_dataset_516204

The Dataset's Variables and Attributes

Row Type	Variable Name	Attribute Name	Data Type	Value
attribute	NC_GLOBAL	access_formats	String	.htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute	NC_GLOBAL	acquisition_description	String	Samples were collected from the CTD-Rosette PVC Niskin bottles, then preserved with paraformaldehyde (0.5% final concentration). Preserved samples were then flash frozen in liquid nitrogen, and subsequently stored at -80\u00b0C until analysis on-shore in batches. Upon thawing, samples were stained with Hoechst 33342 DNA stain (1 ug/mL final concentration), incubated at room temperature in the dark for 1 h, then analyzed using a Beckman-Coulter Altra flow cytometer, equipped with one 488 nm 1W laser and one UV 200 mM laser for excitation. Samples (100 ul) were delivered quantitatively to the instrument using a Harvard Apparatus syringe pump, at a rate of 50 ul per minute. Signals from the DNA detector (450\u00b140 nm) and the forward and side scatter detectors were used to delineate non-pigmented bacteria, in conjunction with the Chlorophyll detector (680\u00b120 nm) and Phycoerythrin detector (575\u00b120 nm) to eliminate chlorophyll and/or phycoerythrin-bearing cells that overlapped (e.g., Prochlorococcus and Synechoccoccus). Calibration beads (0.5 u\u00b5m and 1.0 um yellow-green and 0.5 um UV) were used to normalize cellular fluorescence values and assure optimal instrument settings. Raw data files (listmode) were processed in FlowJo software (Treestar Inc.).
attribute	NC_GLOBAL	awards_0_award_nid	String	515386
attribute	NC_GLOBAL	awards_0_award_number	String	OCE-0826626
attribute	NC_GLOBAL	awards_0_data_url	String	http://www.nsf.gov/awardsearch/showAward?AWD_ID=0826626
attribute	NC_GLOBAL	awards_0_funder_name	String	NSF Division of Ocean Sciences
attribute	NC_GLOBAL	awards_0_funding_acronym	String	NSF OCE
attribute	NC_GLOBAL	awards_0_funding_source_nid	String	355
attribute	NC_GLOBAL	awards_0_program_manager	String	David L. Garrison
attribute	NC_GLOBAL	awards_0_program_manager_nid	String	50534
attribute	NC_GLOBAL	cdm_data_type	String	Other
attribute	NC_GLOBAL	comment	String	Heterotrophic (non-pigmented) bacteria abundance (on-shore analysis) PI: Michael Landry (UCSD-SIO) Contact: Karen Selph (UH) Version: 30 May 2014
attribute	NC_GLOBAL	Conventions	String	COARDS, CF-1.6, ACDD-1.3
attribute	NC_GLOBAL	creator_email	String	info at bco-dmo.org
attribute	NC_GLOBAL	creator_name	String	BCO-DMO
attribute	NC_GLOBAL	creator_type	String	institution
attribute	NC_GLOBAL	creator_url	String	https://www.bco-dmo.org/
attribute	NC_GLOBAL	data_source	String	extract_data_as_tsv version 2.3 19 Dec 2019
attribute	NC_GLOBAL	date_created	String	2014-05-30T17:19:48Z
attribute	NC_GLOBAL	date_modified	String	2019-12-11T19:57:15Z
attribute	NC_GLOBAL	defaultDataQuery	String	&time<now
attribute	NC_GLOBAL	doi	String	10.1575/1912/bco-dmo.516204.1
attribute	NC_GLOBAL	Easternmost_Easting	double	-86.735
attribute	NC_GLOBAL	geospatial_lat_max	double	10.2998
attribute	NC_GLOBAL	geospatial_lat_min	double	6.6219
attribute	NC_GLOBAL	geospatial_lat_units	String	degrees_north
attribute	NC_GLOBAL	geospatial_lon_max	double	-86.735
attribute	NC_GLOBAL	geospatial_lon_min	double	-92.9871
attribute	NC_GLOBAL	geospatial_lon_units	String	degrees_east
attribute	NC_GLOBAL	geospatial_vertical_max	double	120.0
attribute	NC_GLOBAL	geospatial_vertical_min	double	2.0
attribute	NC_GLOBAL	geospatial_vertical_positive	String	down
attribute	NC_GLOBAL	geospatial_vertical_units	String	m
attribute	NC_GLOBAL	infoUrl	String	https://www.bco-dmo.org/dataset/516204
attribute	NC_GLOBAL	institution	String	BCO-DMO
attribute	NC_GLOBAL	instruments_0_acronym	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_dataset_instrument_description	String	Samples were collected from the CTD-Rosette PVC Niskin bottles.
attribute	NC_GLOBAL	instruments_0_dataset_instrument_nid	String	516233
attribute	NC_GLOBAL	instruments_0_description	String	A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24 or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute	NC_GLOBAL	instruments_0_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
attribute	NC_GLOBAL	instruments_0_instrument_name	String	Niskin bottle
attribute	NC_GLOBAL	instruments_0_instrument_nid	String	413
attribute	NC_GLOBAL	instruments_0_supplied_name	String	Niskin bottle
attribute	NC_GLOBAL	instruments_1_acronym	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_1_dataset_instrument_description	String	Samples were run on a Beckman-Coulter Altra flow cytometer, equipped with one 488 nm 1W laser and one UV 200 mM laser for excitation.
attribute	NC_GLOBAL	instruments_1_dataset_instrument_nid	String	516232
attribute	NC_GLOBAL	instruments_1_description	String	Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. (from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute	NC_GLOBAL	instruments_1_instrument_external_identifier	String	https://vocab.nerc.ac.uk/collection/L05/current/LAB37/
attribute	NC_GLOBAL	instruments_1_instrument_name	String	Flow Cytometer
attribute	NC_GLOBAL	instruments_1_instrument_nid	String	660
attribute	NC_GLOBAL	instruments_1_supplied_name	String	Beckman-Coulter Altra flow cytometer
attribute	NC_GLOBAL	keywords	String	bact, bact_het_cyt, bco, bco-dmo, biological, cast, chemical, cycle, cyt, data, dataset, date, date_local, depth, dmo, erddap, event, het, latitude, local, longitude, management, niskin, oceanography, office, preliminary
attribute	NC_GLOBAL	license	String	https://www.bco-dmo.org/dataset/516204/license
attribute	NC_GLOBAL	metadata_source	String	https://www.bco-dmo.org/api/dataset/516204
attribute	NC_GLOBAL	Northernmost_Northing	double	10.2998
attribute	NC_GLOBAL	param_mapping	String	{'516204': {'lat': 'master - latitude', 'depth': 'flag - depth', 'lon': 'master - longitude'}}
attribute	NC_GLOBAL	parameter_source	String	https://www.bco-dmo.org/mapserver/dataset/516204/parameters
attribute	NC_GLOBAL	people_0_affiliation	String	University of California-San Diego
attribute	NC_GLOBAL	people_0_affiliation_acronym	String	UCSD-SIO
attribute	NC_GLOBAL	people_0_person_name	String	Michael R. Landry
attribute	NC_GLOBAL	people_0_person_nid	String	50545
attribute	NC_GLOBAL	people_0_role	String	Principal Investigator
attribute	NC_GLOBAL	people_0_role_type	String	originator
attribute	NC_GLOBAL	people_1_affiliation	String	University of Hawaii at Manoa
attribute	NC_GLOBAL	people_1_affiliation_acronym	String	SOEST
attribute	NC_GLOBAL	people_1_person_name	String	Karen Selph
attribute	NC_GLOBAL	people_1_person_nid	String	50848
attribute	NC_GLOBAL	people_1_role	String	Contact
attribute	NC_GLOBAL	people_1_role_type	String	related
attribute	NC_GLOBAL	people_2_affiliation	String	Woods Hole Oceanographic Institution
attribute	NC_GLOBAL	people_2_affiliation_acronym	String	WHOI BCO-DMO
attribute	NC_GLOBAL	people_2_person_name	String	Shannon Rauch
attribute	NC_GLOBAL	people_2_person_nid	String	51498
attribute	NC_GLOBAL	people_2_role	String	BCO-DMO Data Manager
attribute	NC_GLOBAL	people_2_role_type	String	related
attribute	NC_GLOBAL	project	String	CRD FLUZiE
attribute	NC_GLOBAL	projects_0_acronym	String	CRD FLUZiE
attribute	NC_GLOBAL	projects_0_description	String	Research was aimed at improved understanding of plankton dynamics, carbon and nutrient fluxes, and potential trace element limitation in the Costa Rica Dome region of the eastern tropical Pacific. The specific science objectives of the 2010 R/V Melville cruise (MV1008) were: 1) to assess grazing and trace metal/nutrient controls on primary production and phytoplankton standing stocks; 2) to quantify carbon and elemental fluxes and export rates from the euphotic zone; and 3) to measure microbial population, processes, stable isotope abundances associated with the OMZ and nitrite maxima. Additional information about MV1008 can be found in the cruise report (PDF). NOTE: The original proposal and award abstract are not relevant. The project was originally funded by NSF as experimental tests of phytoplankton controls in the Arabian Sea. Piracy concerns in the region led to the cancellation of the research cruise in 2009, and a Change of Scope request was approved to focus the project on related issues in the Costa Rica Dome (CRD). Though this project is not formally affiliated with any large program, it aligns with IMBER's emphasis on community ecology and biogeochemistry, and the OCB focus on carbon-based measurements of production, grazing and export processes.
attribute	NC_GLOBAL	projects_0_end_date	String	2013-12
attribute	NC_GLOBAL	projects_0_geolocation	String	Costa Rica Dome, Eastern Tropical Pacific Ocean
attribute	NC_GLOBAL	projects_0_name	String	Costa Rica Dome FLUx and Zinc Experiments
attribute	NC_GLOBAL	projects_0_project_nid	String	515387
attribute	NC_GLOBAL	projects_0_start_date	String	2009-01
attribute	NC_GLOBAL	publisher_name	String	Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute	NC_GLOBAL	publisher_type	String	institution
attribute	NC_GLOBAL	sourceUrl	String	(local files)
attribute	NC_GLOBAL	Southernmost_Northing	double	6.6219
attribute	NC_GLOBAL	standard_name_vocabulary	String	CF Standard Name Table v55
attribute	NC_GLOBAL	summary	String	Non-pigmented bacteria abundances from preserved (0.5% paraformaldehyde) frozen samples run on a Beckman-Coulter Altra flow cytometer after staining with Hoechst 33342 DNA stain and excitation with 200 mW UV and 1W 488 nm laser light. Bacteria discriminated based on DNA content (450\u00b140 nm), as well as forward and 90\u00b0 side light scatter parameters. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean.
attribute	NC_GLOBAL	title	String	[bacteria_FCM_lab] - Heterotrophic (non-pigmented) bacteria abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project) (Costa Rica Dome FLUx and Zinc Experiments)
attribute	NC_GLOBAL	version	String	1
attribute	NC_GLOBAL	Westernmost_Easting	double	-92.9871
attribute	NC_GLOBAL	xml_source	String	osprey2erddap.update_xml() v1.3
variable	event		short
attribute	event	_FillValue	short	32767
attribute	event	actual_range	short	17, 1111
attribute	event	bcodmo_name	String	event
attribute	event	description	String	Number referring to the particular activity (event) on the FluZiE cruise.
attribute	event	long_name	String	Event
attribute	event	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/EVTAGFL/
attribute	event	units	String	integer
variable	cast		byte
attribute	cast	_FillValue	byte	127
attribute	cast	actual_range	byte	3, 85
attribute	cast	bcodmo_name	String	cast
attribute	cast	description	String	CTD Cast number from the FluZiE cruise.
attribute	cast	long_name	String	Cast
attribute	cast	units	String	integer
variable	cycle		String
attribute	cycle	bcodmo_name	String	unknown
attribute	cycle	description	String	Type and number of cruise sampling event. Either "Stn_n" or "Cycle_n". A transect of stations was sampled from 29 June to 03 July. Five quasi-Lagrangian experiments called "cycles" were conducted during the remainder of the cruise.
attribute	cycle	long_name	String	Cycle
attribute	cycle	units	String	text
variable	date_local		String
attribute	date_local	bcodmo_name	String	date
attribute	date_local	description	String	Date of CTD cast (local time zone of UTC -6). format: mmddyyyy
attribute	date_local	long_name	String	Date Local
attribute	date_local	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/
attribute	date_local	units	String	unitless
variable	latitude		double
attribute	latitude	_CoordinateAxisType	String	Lat
attribute	latitude	_FillValue	double	NaN
attribute	latitude	actual_range	double	6.6219, 10.2998
attribute	latitude	axis	String	Y
attribute	latitude	bcodmo_name	String	latitude
attribute	latitude	colorBarMaximum	double	90.0
attribute	latitude	colorBarMinimum	double	-90.0
attribute	latitude	description	String	Latitude in degrees North.
attribute	latitude	ioos_category	String	Location
attribute	latitude	long_name	String	Latitude
attribute	latitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LATX/
attribute	latitude	standard_name	String	latitude
attribute	latitude	units	String	degrees_north
variable	longitude		double
attribute	longitude	_CoordinateAxisType	String	Lon
attribute	longitude	_FillValue	double	NaN
attribute	longitude	actual_range	double	-92.9871, -86.735
attribute	longitude	axis	String	X
attribute	longitude	bcodmo_name	String	longitude
attribute	longitude	colorBarMaximum	double	180.0
attribute	longitude	colorBarMinimum	double	-180.0
attribute	longitude	description	String	Longitude in degrees East.
attribute	longitude	ioos_category	String	Location
attribute	longitude	long_name	String	Longitude
attribute	longitude	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/LONX/
attribute	longitude	standard_name	String	longitude
attribute	longitude	units	String	degrees_east
variable	depth		double
attribute	depth	_CoordinateAxisType	String	Height
attribute	depth	_CoordinateZisPositive	String	down
attribute	depth	_FillValue	double	NaN
attribute	depth	actual_range	double	2.0, 120.0
attribute	depth	axis	String	Z
attribute	depth	bcodmo_name	String	depth
attribute	depth	colorBarMaximum	double	8000.0
attribute	depth	colorBarMinimum	double	-8000.0
attribute	depth	colorBarPalette	String	TopographyDepth
attribute	depth	description	String	Sample depth.
attribute	depth	ioos_category	String	Location
attribute	depth	long_name	String	Depth
attribute	depth	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P09/current/DEPH/
attribute	depth	positive	String	down
attribute	depth	standard_name	String	depth
attribute	depth	units	String	m
variable	niskin		byte
attribute	niskin	_FillValue	byte	127
attribute	niskin	actual_range	byte	1, 22
attribute	niskin	bcodmo_name	String	bot_Nis
attribute	niskin	description	String	Niskin bottle that the sample was taken from.
attribute	niskin	long_name	String	Niskin
attribute	niskin	units	String	integer
variable	bact_het_cyt		int
attribute	bact_het_cyt	_FillValue	int	2147483647
attribute	bact_het_cyt	actual_range	int	22569, 1706696
attribute	bact_het_cyt	bcodmo_name	String	bact_het_cyt
attribute	bact_het_cyt	description	String	Heterotrophic bacteria (non-pigmented bacteria) abundance.
attribute	bact_het_cyt	long_name	String	Bact Het Cyt
attribute	bact_het_cyt	nerc_identifier	String	https://vocab.nerc.ac.uk/collection/P01/current/HBCCXXXX/
attribute	bact_het_cyt	units	String	cells per milliliter (cells/mL)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.