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Title Sum-
Institution Dataset ID
     data   graph     files  public Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis)
from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD
FLUZiE project)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson
attribute NC_GLOBAL acquisition_description String Samples were collected from the CTD-Rosette PVC Niskin bottles, then preserved
with paraformaldehyde (0.5% final concentration). Preserved samples were then
flash frozen in liquid nitrogen, and subsequently stored at -80\u00b0C until
analysis on-shore in batches. Upon thawing, samples were stained with Hoechst
33342 DNA stain (1 ug/mL final concentration), incubated at room temperature
in the dark for 1 h, then analyzed using a Beckman-Coulter Altra flow
cytometer, equipped with one 488 nm 1W laser and one UV 200 mM laser for
excitation. Samples (100 ul) were delivered quantitatively to the instrument
using a Harvard Apparatus syringe pump, at a rate of 50 ul per minute. Signals
from the DNA (450\u00b140 nm), Chlorophyll (680\u00b120 nm), Phycoerythrin
(575\u00b120 nm), and the forward and side scatter detectors were used to
delineate Prochlorococcus, Synechococcus, and Picophytoeukaryotes. Calibration
beads (0.5 um and 1.0 um yellow-green and 0.5 um UV) were used to normalize
cellular fluorescence values and assure optimal instrument settings. Raw data
files (listmode) were processed in FlowJo software (Treestar Inc.).
attribute NC_GLOBAL awards_0_award_nid String 515386
attribute NC_GLOBAL awards_0_award_number String OCE-0826626
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward?AWD_ID=0826626 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String Picophytoplankton abundance (on-shore analysis)
PI: Michael Landry (UCSD-SIO)
Contact: Karen Selph (UH)
Version: 02 June 2014
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2014-06-02T14:47:32Z
attribute NC_GLOBAL date_modified String 2019-12-27T19:02:15Z
attribute NC_GLOBAL defaultDataQuery String &time<now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.516296.1
attribute NC_GLOBAL Easternmost_Easting double -86.735
attribute NC_GLOBAL geospatial_lat_max double 10.2998
attribute NC_GLOBAL geospatial_lat_min double 6.6219
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -86.735
attribute NC_GLOBAL geospatial_lon_min double -92.9871
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 120.0
attribute NC_GLOBAL geospatial_vertical_min double 2.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/516296 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Niskin bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_description String Samples were collected from the CTD-Rosette PVC Niskin bottles.
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 516304
attribute NC_GLOBAL instruments_0_description String A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24 or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Niskin bottle
attribute NC_GLOBAL instruments_0_instrument_nid String 413
attribute NC_GLOBAL instruments_0_supplied_name String Niskin bottle
attribute NC_GLOBAL instruments_1_acronym String Flow Cytometer
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Samples were run on a Beckman-Coulter Altra flow cytometer, equipped with one 488 nm 1W laser and one UV 200 mM laser for excitation.
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 516303
attribute NC_GLOBAL instruments_1_description String Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells.
(from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm)
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB37/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String Flow Cytometer
attribute NC_GLOBAL instruments_1_instrument_nid String 660
attribute NC_GLOBAL instruments_1_supplied_name String Beckman-Coulter Altra flow cytometer
attribute NC_GLOBAL keywords String bco, bco-dmo, biological, cast, chemical, coccus, coccus_p, coccus_s, cycle, data, dataset, date, date_local, depth, dmo, erddap, event, latitude, local, longitude, management, niskin, oceanography, office, peuk, preliminary
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/516296/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/516296 (external link)
attribute NC_GLOBAL Northernmost_Northing double 10.2998
attribute NC_GLOBAL param_mapping String {'516296': {'lat': 'master - latitude', 'depth': 'flag - depth', 'lon': 'master - longitude'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/516296/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String University of California-San Diego
attribute NC_GLOBAL people_0_affiliation_acronym String UCSD-SIO
attribute NC_GLOBAL people_0_person_name String Michael R. Landry
attribute NC_GLOBAL people_0_person_nid String 50545
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String University of Hawaii at Manoa
attribute NC_GLOBAL people_1_affiliation_acronym String SOEST
attribute NC_GLOBAL people_1_person_name String Karen Selph
attribute NC_GLOBAL people_1_person_nid String 50848
attribute NC_GLOBAL people_1_role String Contact
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Shannon Rauch
attribute NC_GLOBAL people_2_person_nid String 51498
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String CRD FLUZiE
attribute NC_GLOBAL projects_0_acronym String CRD FLUZiE
attribute NC_GLOBAL projects_0_description String Research was aimed at improved understanding of plankton dynamics, carbon and nutrient fluxes, and potential trace element limitation in the Costa Rica Dome region of the eastern tropical Pacific. The specific science objectives of the 2010 R/V Melville cruise (MV1008) were:
1) to assess grazing and trace metal/nutrient controls on primary production and phytoplankton standing stocks;
2) to quantify carbon and elemental fluxes and export rates from the euphotic zone; and
3) to measure microbial population, processes, stable isotope abundances associated with the OMZ and nitrite maxima.
Additional information about MV1008 can be found in the cruise report (PDF).
NOTE: The original proposal and award abstract are not relevant. The project was originally funded by NSF as experimental tests of phytoplankton controls in the Arabian Sea. Piracy concerns in the region led to the cancellation of the research cruise in 2009, and a Change of Scope request was approved to focus the project on related issues in the Costa Rica Dome (CRD).
Though this project is not formally affiliated with any large program, it aligns with IMBER's emphasis on community ecology and biogeochemistry, and the OCB focus on carbon-based measurements of production, grazing and export processes.
attribute NC_GLOBAL projects_0_end_date String 2013-12
attribute NC_GLOBAL projects_0_geolocation String Costa Rica Dome, Eastern Tropical Pacific Ocean
attribute NC_GLOBAL projects_0_name String Costa Rica Dome FLUx and Zinc Experiments
attribute NC_GLOBAL projects_0_project_nid String 515387
attribute NC_GLOBAL projects_0_start_date String 2009-01
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 6.6219
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Picophytoplankton abundances from preserved (0.5% paraformaldehyde) frozen samples run on a Beckman-Coulter Altra flow cytometer after staining with Hoechst 33342 DNA stain and excitation with 200 mW UV and 1W 488 nm laser light. Cell populations include Prochlorococcus, Synechococcus, and Picophytoeukaryotes. Samples were collected on the MV1008 cruise in the Costa Rica Dome (CRD) region of the Eastern Tropical Pacific Ocean.
attribute NC_GLOBAL title String Picophytoplankton abundances determined by flow cytometry (post-cruise/on-shore analysis) from samples collected on R/V Melville cruise MV1008 in the Costa Rica Dome in 2010 (CRD FLUZiE project)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -92.9871
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable event   short  
attribute event _FillValue short 32767
attribute event actual_range short 17, 1111
attribute event bcodmo_name String event
attribute event description String Number referring to the particular activity (event) on the FluZiE cruise.
attribute event long_name String Event
attribute event nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/EVTAGFL/ (external link)
attribute event units String integer
variable cast   byte  
attribute cast _FillValue byte 127
attribute cast actual_range byte 3, 85
attribute cast bcodmo_name String cast
attribute cast description String CTD Cast number from the FluZiE cruise.
attribute cast long_name String Cast
attribute cast units String integer
variable cycle   String  
attribute cycle bcodmo_name String unknown
attribute cycle description String Type and number of cruise sampling event. Either "Stn_n" or "Cycle_n". A transect of stations was sampled from 29 June to 03 July. Five quasi-Lagrangian experiments called "cycles" were conducted during the remainder of the cruise.
attribute cycle long_name String Cycle
attribute cycle units String text
variable date_local   String  
attribute date_local bcodmo_name String date
attribute date_local description String Date of CTD cast (local time zone of UTC -6). in the format mmddyyyy
attribute date_local long_name String Date Local
attribute date_local nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/ADATAA01/ (external link)
attribute date_local units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 6.6219, 10.2998
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude in degrees North.
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -92.9871, -86.735
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude in degrees East.
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 2.0, 120.0
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String Sample depth.
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable niskin   byte  
attribute niskin _FillValue byte 127
attribute niskin actual_range byte 1, 22
attribute niskin bcodmo_name String bot_Nis
attribute niskin description String Niskin bottle that the sample was taken from.
attribute niskin long_name String Niskin
attribute niskin units String integer
variable coccus_p   int  
attribute coccus_p _FillValue int 2147483647
attribute coccus_p actual_range int 1324, 559704
attribute coccus_p bcodmo_name String coccus_p
attribute coccus_p description String Abundance of Prochlorococcus abundance.
attribute coccus_p long_name String Coccus P
attribute coccus_p nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/P701A90Z/ (external link)
attribute coccus_p units String cells per milliliter (cells/mL)
variable coccus_s   int  
attribute coccus_s _FillValue int 2147483647
attribute coccus_s actual_range int 75, 308163
attribute coccus_s bcodmo_name String coccus_s
attribute coccus_s description String Abundance of Synechococcus abundance.
attribute coccus_s long_name String Coccus S
attribute coccus_s nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/PATX/ (external link)
attribute coccus_s units String cells per milliliter (cells/mL)
variable peuk   int  
attribute peuk _FillValue int 2147483647
attribute peuk actual_range int 118, 70301
attribute peuk bcodmo_name String pico_euks
attribute peuk description String Abundance of Picophytoeukaryotes.
attribute peuk long_name String Peuk
attribute peuk nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/PNTX (external link)
attribute peuk units String cells per milliliter (cells/mL)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.

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