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     data   graph     files  public [HPLC Pigments] - HPLC pigment analyses of CTD-collected samples from R/V Knorr cruise KN207-
03 in the North Atlantic (transect from Ponta Delgada, Azores to Reykjavik, Iceland) in
2012 (NA-VICE project) (Lipid lubrication of oceanic carbon and sulfur biogeochemistry via a
host-virus chemical arms race)
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The Dataset's Variables and Attributes

Row Type Variable Name Attribute Name Data Type Value
attribute NC_GLOBAL access_formats String .htmlTable,.csv,.json,.mat,.nc,.tsv,.esriCsv,.geoJson,.odvTxt
attribute NC_GLOBAL acquisition_description String Water samples were collected from CTD Niskin bottles. Water samples for HPLC
analyses were taken from one Niskin bottle per cast-depth combination included
in the dataset. Note that the niskin_sampled column indicates which Niskin
bottle the HPLC sample was taken from. The niskins_fired column indicates all
Niskin bottle numbers fired at the specified depth, though the HPLC sample was
taken from only one of those bottles.

Chlorophyll and accessory pigment composition was analyzed by high performance
liquid chromatography (HPLC; Agilent 1100). Culture aliquots were filtered on
Whatmann GF/F filters, flash frozen in liquid nitrogen, and stored at
-80\u00b0C until analysis. Just prior to analysis, pigments were extracted
overnight in acetone at -20\u00b0C. The following day extracted pigments were
centrifuged and measured using a gradient elution method (DiTullio and Geesey,
2003), a modification of the Zapata et al 2000 method. Chromatographic
separation was performed using a Waters C8 symmetry column, photodiode array
and fluorescence detectors. The internal standard, \u03b2-Apo-8-carotenal-
trans standard (Fluka Chemical Corp., USA) was added to extracted pigments as
a peak reference. Individual pigment peaks were quantified with Chemstation
software (revision B.03.01, Agilent) and our pigment action spectra library
calibrated using pigment standards from DHI LABS (Hoersholm, Denmark) and in-
house purifications of non-commercially available pigments. Coefficient of
variation among replicate HPLC injections is < 3% and our limit of detection
is approximately 1 ng L-1.
attribute NC_GLOBAL awards_0_award_nid String 54870
attribute NC_GLOBAL awards_0_award_number String OCE-1061876
attribute NC_GLOBAL awards_0_data_url String http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=1061876 (external link)
attribute NC_GLOBAL awards_0_funder_name String NSF Division of Ocean Sciences
attribute NC_GLOBAL awards_0_funding_acronym String NSF OCE
attribute NC_GLOBAL awards_0_funding_source_nid String 355
attribute NC_GLOBAL awards_0_program_manager String David L. Garrison
attribute NC_GLOBAL awards_0_program_manager_nid String 50534
attribute NC_GLOBAL cdm_data_type String Other
attribute NC_GLOBAL comment String HPLC Pigments
PI: Giacomo R. DiTullio
Contact: Emily Cooper
Version History:
Current version: 17 Sept 2014
Dataset revised: 12 Sept 2014
Original dataset: 26 June 2014
attribute NC_GLOBAL Conventions String COARDS, CF-1.6, ACDD-1.3
attribute NC_GLOBAL creator_email String info at bco-dmo.org
attribute NC_GLOBAL creator_name String BCO-DMO
attribute NC_GLOBAL creator_type String institution
attribute NC_GLOBAL creator_url String https://www.bco-dmo.org/ (external link)
attribute NC_GLOBAL data_source String extract_data_as_tsv version 2.3 19 Dec 2019
attribute NC_GLOBAL date_created String 2014-06-26T15:57:25Z
attribute NC_GLOBAL date_modified String 2019-12-30T15:40:59Z
attribute NC_GLOBAL defaultDataQuery String &amp;time&lt;now
attribute NC_GLOBAL doi String 10.1575/1912/bco-dmo.517634.1
attribute NC_GLOBAL Easternmost_Easting double -26.102033
attribute NC_GLOBAL geospatial_lat_max double 65.447967
attribute NC_GLOBAL geospatial_lat_min double 42.963
attribute NC_GLOBAL geospatial_lat_units String degrees_north
attribute NC_GLOBAL geospatial_lon_max double -26.102033
attribute NC_GLOBAL geospatial_lon_min double -35.0733
attribute NC_GLOBAL geospatial_lon_units String degrees_east
attribute NC_GLOBAL geospatial_vertical_max double 150.0
attribute NC_GLOBAL geospatial_vertical_min double 3.0
attribute NC_GLOBAL geospatial_vertical_positive String down
attribute NC_GLOBAL geospatial_vertical_units String m
attribute NC_GLOBAL infoUrl String https://www.bco-dmo.org/dataset/517634 (external link)
attribute NC_GLOBAL institution String BCO-DMO
attribute NC_GLOBAL instruments_0_acronym String Niskin bottle
attribute NC_GLOBAL instruments_0_dataset_instrument_nid String 517688
attribute NC_GLOBAL instruments_0_description String A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24 or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc.
attribute NC_GLOBAL instruments_0_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/ (external link)
attribute NC_GLOBAL instruments_0_instrument_name String Niskin bottle
attribute NC_GLOBAL instruments_0_instrument_nid String 413
attribute NC_GLOBAL instruments_0_supplied_name String Niskin bottle
attribute NC_GLOBAL instruments_1_acronym String HPLC
attribute NC_GLOBAL instruments_1_dataset_instrument_description String Chlorophyll and accessory pigment composition was analyzed by high performance liquid chromatography (HPLC; Agilent 1100).
attribute NC_GLOBAL instruments_1_dataset_instrument_nid String 517637
attribute NC_GLOBAL instruments_1_description String A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.
attribute NC_GLOBAL instruments_1_instrument_external_identifier String https://vocab.nerc.ac.uk/collection/L05/current/LAB11/ (external link)
attribute NC_GLOBAL instruments_1_instrument_name String High Performance Liquid Chromatograph
attribute NC_GLOBAL instruments_1_instrument_nid String 506
attribute NC_GLOBAL instruments_1_supplied_name String HPLC
attribute NC_GLOBAL keywords String amb, amb_bot, bco, bco-dmo, biological, bot, cast, chemical, concentration, data, dataset, date, date_gmt, date_local, depth, dmo, erddap, filt, filt_vol, fired, iso, ISO_DateTime_UTC, lat_deg, lat_min, latitude, local, lon_deg, lon_min, longitude, management, niskin, niskin_sampled, niskins, niskins_fired, oceanography, office, pigment, preliminary, sampled, station, time, time_gmt, time_local, vol
attribute NC_GLOBAL license String https://www.bco-dmo.org/dataset/517634/license (external link)
attribute NC_GLOBAL metadata_source String https://www.bco-dmo.org/api/dataset/517634 (external link)
attribute NC_GLOBAL Northernmost_Northing double 65.447967
attribute NC_GLOBAL param_mapping String {'517634': {'lat': 'flag - latitude', 'depth': 'flag - depth', 'lon': 'flag - longitude', 'ISO_DateTime_UTC': 'flag - time'}}
attribute NC_GLOBAL parameter_source String https://www.bco-dmo.org/mapserver/dataset/517634/parameters (external link)
attribute NC_GLOBAL people_0_affiliation String Grice Marine Laboratory - College of Charleston
attribute NC_GLOBAL people_0_affiliation_acronym String GML-CoC
attribute NC_GLOBAL people_0_person_name String Giacomo DiTullio
attribute NC_GLOBAL people_0_person_nid String 50689
attribute NC_GLOBAL people_0_role String Principal Investigator
attribute NC_GLOBAL people_0_role_type String originator
attribute NC_GLOBAL people_1_affiliation String Grice Marine Laboratory - College of Charleston
attribute NC_GLOBAL people_1_affiliation_acronym String GML-CoC
attribute NC_GLOBAL people_1_person_name String Emily Cooper
attribute NC_GLOBAL people_1_person_nid String 517640
attribute NC_GLOBAL people_1_role String Contact
attribute NC_GLOBAL people_1_role_type String related
attribute NC_GLOBAL people_2_affiliation String Woods Hole Oceanographic Institution
attribute NC_GLOBAL people_2_affiliation_acronym String WHOI BCO-DMO
attribute NC_GLOBAL people_2_person_name String Shannon Rauch
attribute NC_GLOBAL people_2_person_nid String 51498
attribute NC_GLOBAL people_2_role String BCO-DMO Data Manager
attribute NC_GLOBAL people_2_role_type String related
attribute NC_GLOBAL project String NA-VICE
attribute NC_GLOBAL projects_0_acronym String NA-VICE
attribute NC_GLOBAL projects_0_description String This project is also called "NA-VICE" (North Atlantic Virus Infection of Coccolithophores Expedition).
Project description from NSF award abstract:
Despite the critical importance of viruses in shaping marine microbial ecosystems, very little is known about the molecular mechanisms mediating phytoplankton-virus interactions. As a consequence, we currently lack biomarkers to quantify active viral infection in the oceans, significantly hindering our understanding of its ecological and biogeochemical impacts.
The coccolithophore Emiliania huxleyi (Prymnesiophyceae, Haptophyte) is a cosmopolitan unicellular photoautotroph whose calcite skeletons account for about a third of the total marine CaCO3 production. E. huxleyi forms massive annual spring blooms in the North Atlantic that are infected and terminated by lytic, giant double-stranded DNA containing coccolithoviruses. Findings that lytic viral infection of E. huxleyi recruits the hosts programmed cell death (PCD) machinery demonstrate that viruses employ a sophisticated, co-evolutionary “arms race” in mediating host-virus interactions. The investigators recently demonstrated that viral glycosphingolipids (vGSLs), derived from unexpected cluster of sphingolipid biosynthetic genes, a pathway never before described in a viral genome, play a crucial functional role in facilitating infection of E. huxleyi. The observations of vGSLs in the North Atlantic and Norwegian fjords further suggest that they may be novel, diagnostic biomarkers for viral infection of coccolithophore populations. At the same time, the discovery of vGSLs and a distinct, protective 802 lipid argues that a host-virus, co-evolutionary chemical arms race plays a pivotal role in regulating viral infection and in lubricating upper ocean biogeochemical fluxes of carbon and sulfur.
The focus of this collaborative research project is to elucidate the molecular, ecological, and biogeochemical links between vGSLs (and other polar lipids) and the global cycles of carbon and sulfur.
The team of investigators proposes a multi-pronged approach combing a suite of lab-based, mechanistic studies using several haptophyte-virus model systems along with observational studies and manipulative field-based experiments the Northeast Atlantic. Using these diagnostic markers, they will document active viral infection of natural coccolithophore populations and couple it with a suite of oceanographic measurements in order to quantify how viral infection (via vGSLs) influences cell fate, the dissolved organic carbon (DOC) pool, vertical export of particular organic (POC) and inorganic carbon (PIC; as calcium carbonate, CaCO3) (along with associated alkenone lipid biomarkers and genetic signatures of viruses and their hosts) and the upper ocean sulfur cycle (via the cycling of dimethylsulfide [DMS] and other biogenic sulfur compounds). Furthermore, given they are unique to viruses, the investigators propose that vGSLs can be used to trace the flow of virally-derived carbon and provide quantitative insights into a “viral shunt” that diverts fixed carbon from higher trophic levels and the deep sea.
The overarching hypothesis for this study is that vGSLs are cornerstone molecules in the upper ocean, which facilitate viral infection on massive scales and thereby mechanistically "lubricate" the biogeochemical fluxes of C and S in the ocean.
attribute NC_GLOBAL projects_0_end_date String 2014-02
attribute NC_GLOBAL projects_0_geolocation String North Atlantic; Azores to Iceland
attribute NC_GLOBAL projects_0_name String Lipid lubrication of oceanic carbon and sulfur biogeochemistry via a host-virus chemical arms race
attribute NC_GLOBAL projects_0_project_nid String 2136
attribute NC_GLOBAL projects_0_start_date String 2011-03
attribute NC_GLOBAL publisher_name String Biological and Chemical Oceanographic Data Management Office (BCO-DMO)
attribute NC_GLOBAL publisher_type String institution
attribute NC_GLOBAL sourceUrl String (local files)
attribute NC_GLOBAL Southernmost_Northing double 42.963
attribute NC_GLOBAL standard_name_vocabulary String CF Standard Name Table v55
attribute NC_GLOBAL summary String Dataset includes HPLC pigment analyses of CTD collected samples from the KN207-03 cruise (Northeast Atlantic Ocean transect from Azores to Iceland).
attribute NC_GLOBAL time_coverage_start String 2012-06-17T11:00:00Z
attribute NC_GLOBAL title String [HPLC Pigments] - HPLC pigment analyses of CTD-collected samples from R/V Knorr cruise KN207-03 in the North Atlantic (transect from Ponta Delgada, Azores to Reykjavik, Iceland) in 2012 (NA-VICE project) (Lipid lubrication of oceanic carbon and sulfur biogeochemistry via a host-virus chemical arms race)
attribute NC_GLOBAL version String 1
attribute NC_GLOBAL Westernmost_Easting double -35.0733
attribute NC_GLOBAL xml_source String osprey2erddap.update_xml() v1.3
variable cast   String  
attribute cast bcodmo_name String cast
attribute cast description String Cast number.
attribute cast long_name String Cast
attribute cast units String alphanumeric
variable station   String  
attribute station bcodmo_name String station
attribute station description String Station name.
attribute station long_name String Station
attribute station units String text
variable date_gmt   String  
attribute date_gmt bcodmo_name String date_gmt
attribute date_gmt description String Date of cast (GMT). in the format mmddyyyy
attribute date_gmt long_name String Date Gmt
attribute date_gmt units String unitless
variable time_gmt   String  
attribute time_gmt bcodmo_name String time_gmt
attribute time_gmt description String Time of cast (GMT). in the format hhmmss
attribute time_gmt long_name String Time Gmt
attribute time_gmt units String unitless
variable date_local   String  
attribute date_local bcodmo_name String date_local
attribute date_local description String Date of cast (local time zone). in the format mmddyyyy
attribute date_local long_name String Date Local
attribute date_local units String unitless
variable time_local   String  
attribute time_local bcodmo_name String time_local
attribute time_local description String Time of cast (local time zone). in the format hhmmss
attribute time_local long_name String Time Local
attribute time_local units String unitless
variable latitude   double  
attribute latitude _CoordinateAxisType String Lat
attribute latitude _FillValue double NaN
attribute latitude actual_range double 42.963, 65.447967
attribute latitude axis String Y
attribute latitude bcodmo_name String latitude
attribute latitude colorBarMaximum double 90.0
attribute latitude colorBarMinimum double -90.0
attribute latitude description String Latitude. Positive values = North.
attribute latitude ioos_category String Location
attribute latitude long_name String Latitude
attribute latitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LATX/ (external link)
attribute latitude standard_name String latitude
attribute latitude units String degrees_north
variable longitude   double  
attribute longitude _CoordinateAxisType String Lon
attribute longitude _FillValue double NaN
attribute longitude actual_range double -35.0733, -26.102033
attribute longitude axis String X
attribute longitude bcodmo_name String longitude
attribute longitude colorBarMaximum double 180.0
attribute longitude colorBarMinimum double -180.0
attribute longitude description String Longitude. Positive values = East.
attribute longitude ioos_category String Location
attribute longitude long_name String Longitude
attribute longitude nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/LONX/ (external link)
attribute longitude standard_name String longitude
attribute longitude units String degrees_east
variable lat_deg   byte  
attribute lat_deg _FillValue byte 127
attribute lat_deg actual_range byte 42, 65
attribute lat_deg bcodmo_name String lat_degs
attribute lat_deg colorBarMaximum double 90.0
attribute lat_deg colorBarMinimum double -90.0
attribute lat_deg description String Degrees latitude.
attribute lat_deg long_name String Latitude
attribute lat_deg standard_name String latitude
attribute lat_deg units String degrees North
variable lat_min   float  
attribute lat_min _FillValue float NaN
attribute lat_min actual_range float 0.0, 59.83
attribute lat_min bcodmo_name String lat_mins
attribute lat_min colorBarMaximum double 90.0
attribute lat_min colorBarMinimum double -90.0
attribute lat_min description String Minutes latitude.
attribute lat_min long_name String Latitude
attribute lat_min standard_name String latitude
attribute lat_min units String minutes North
variable lon_deg   byte  
attribute lon_deg _FillValue byte 127
attribute lon_deg actual_range byte 26, 35
attribute lon_deg bcodmo_name String lon_degs
attribute lon_deg colorBarMaximum double 180.0
attribute lon_deg colorBarMinimum double -180.0
attribute lon_deg description String Degrees longitude. Positive = West.
attribute lon_deg long_name String Longitude
attribute lon_deg standard_name String longitude
attribute lon_deg units String degrees West
variable lon_min   float  
attribute lon_min _FillValue float NaN
attribute lon_min actual_range float 0.0, 56.884
attribute lon_min bcodmo_name String lon_mins
attribute lon_min colorBarMaximum double 180.0
attribute lon_min colorBarMinimum double -180.0
attribute lon_min description String Minutes longitude. Positive = West.
attribute lon_min long_name String Longitude
attribute lon_min standard_name String longitude
attribute lon_min units String minutes West
variable time   double  
attribute time _CoordinateAxisType String Time
attribute time actual_range double 1.3399308E9, NaN
attribute time axis String T
attribute time bcodmo_name String ISO_DateTime_UTC
attribute time description String Date and time of cast formatted to ISO8601 standard.
attribute time ioos_category String Time
attribute time long_name String ISO Date Time UTC
attribute time nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/DTUT8601/ (external link)
attribute time standard_name String time
attribute time time_origin String 01-JAN-1970 00:00:00
attribute time units String seconds since 1970-01-01T00:00:00Z
variable depth   double  
attribute depth _CoordinateAxisType String Height
attribute depth _CoordinateZisPositive String down
attribute depth _FillValue double NaN
attribute depth actual_range double 3.0, 150.0
attribute depth axis String Z
attribute depth bcodmo_name String depth
attribute depth colorBarMaximum double 8000.0
attribute depth colorBarMinimum double -8000.0
attribute depth colorBarPalette String TopographyDepth
attribute depth description String Depth of Niskin bottle firing.
attribute depth ioos_category String Location
attribute depth long_name String Depth
attribute depth nerc_identifier String https://vocab.nerc.ac.uk/collection/P09/current/DEPH/ (external link)
attribute depth positive String down
attribute depth standard_name String depth
attribute depth units String m
variable amb_bot   String  
attribute amb_bot bcodmo_name String bottle
attribute amb_bot description String Amber bottle identification number.
attribute amb_bot long_name String Amb Bot
attribute amb_bot units String integer
variable niskin_sampled   byte  
attribute niskin_sampled _FillValue byte 127
attribute niskin_sampled actual_range byte 1, 24
attribute niskin_sampled bcodmo_name String bottle
attribute niskin_sampled description String Niskin bottle number from which HPLC sample was taken.
attribute niskin_sampled long_name String Niskin Sampled
attribute niskin_sampled units String integer
variable niskins_fired   String  
attribute niskins_fired bcodmo_name String bots
attribute niskins_fired description String Niskin bottle numbers fired at depth; HPLC sample was taken from one Niskin bottle.
attribute niskins_fired long_name String Niskins Fired
attribute niskins_fired units String range of integers
variable filt_vol   float  
attribute filt_vol _FillValue float NaN
attribute filt_vol actual_range float 0.96, 2.15
attribute filt_vol bcodmo_name String fluor_vol_filt
attribute filt_vol description String Volume of water filtered.
attribute filt_vol long_name String Filt Vol
attribute filt_vol nerc_identifier String https://vocab.nerc.ac.uk/collection/P01/current/VOLFMCXX/ (external link)
attribute filt_vol units String liters (L)
variable pigment   String  
attribute pigment bcodmo_name String pigment_concentration
attribute pigment description String Name of the pigment measured:
chl_c3 = Chlorophyll c3;
chl_ide = Chlorophyllide a;
mg_dvp = Magnesium-2;4-divinyl phaeoporphyrin a5 monomethyl ester;
chl_c2 = Chlorophyll c2;
chl_c1 = Chlorophyll c1;
peridinin = Peridinin;
but19 = 19-butanoyloxyfucoxanthin;
fucox = Fucoxanthin;
ph_ide = Pheophorbide a;
neox = Neoxanthin;
prasinox = Prasinoxanthin;
violax = Violaxanthin;
hex19 = 19'-hexanoyloxyfucoxanthin;
diadinox = Diadinoxanthin;
cis_fucox = cis-Fucoxanthin;
allox = Alloxanthin;
diatox = Diatoxanthin;
monad = Monadoxanthin;
zeax = Zeaxanthin;
lutein = lutein;
crocox = Crocoxanthin;
chl_b = Chlorophyll b;
dv_chl_a = Divinyl Chlorophyll a;
chl_a = Chlorophyll a;
p_phytin = Phaeophytin a;
carotene_a = alpha Carotene;
carotene_b = beta Carotene.
attribute pigment long_name String Pigment
attribute pigment nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/OPWC/ (external link)
attribute pigment units String text
variable concentration   short  
attribute concentration _FillValue short 32767
attribute concentration actual_range short 0, 2204
attribute concentration bcodmo_name String pigment_concentration
attribute concentration description String Concentration of the pigment as determined by HPLC.
attribute concentration long_name String Concentration
attribute concentration nerc_identifier String https://vocab.nerc.ac.uk/collection/P02/current/OPWC/ (external link)
attribute concentration units String nanograms per liter (ng/L)

The information in the table above is also available in other file formats (.csv, .htmlTable, .itx, .json, .jsonlCSV1, .jsonlCSV, .jsonlKVP, .mat, .nc, .nccsv, .tsv, .xhtml) via a RESTful web service.


 
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